Development and validation of 697 novel polymorphic genomic and EST-SSR markers in the American cranberry (Vaccinium macrocarpon Ait.)

Publication Overview
TitleDevelopment and validation of 697 novel polymorphic genomic and EST-SSR markers in the American cranberry (Vaccinium macrocarpon Ait.)
AuthorsSchlautman B, Fajardo D, Bougie T, Wiesman E, Polashock J, Vorsa N, Steffan S, Zalapa J
TypeJournal Article
Journal NameMolecules (Basel, Switzerland)
Volume20
Issue2
Year2015
Page(s)2001-13
CitationSchlautman B, Fajardo D, Bougie T, Wiesman E, Polashock J, Vorsa N, Steffan S, Zalapa J. Development and validation of 697 novel polymorphic genomic and EST-SSR markers in the American cranberry (Vaccinium macrocarpon Ait.). Molecules (Basel, Switzerland). 2015; 20(2):2001-13.

Abstract

The American cranberry, Vaccinium macrocarpon Ait., is an economically important North American fruit crop that is consumed because of its unique flavor and potential health benefits. However, a lack of abundant, genome-wide molecular markers has limited the adoption of modern molecular assisted selection approaches in cranberry breeding programs. To increase the number of available markers in the species, this study identified, tested, and validated microsatellite markers from existing nuclear and transcriptome sequencing data. In total, new primers were designed, synthesized, and tested for 979 SSR loci; 697 of the markers amplified allele patterns consistent with single locus segregation in a diploid organism and were considered polymorphic. Of the 697 polymorphic loci, 507 were selected for additional genetic diversity and segregation analyses in 29 cranberry genotypes. More than 95% of the 507 loci did not display segregation distortion at the p < 0.05 level, and contained moderate to high levels of polymorphism with a polymorphic information content >0.25. This comprehensive collection of developed and validated microsatellite loci represents a substantial addition to the molecular tools available for geneticists, genomicists, and breeders in cranberry and Vaccinium.

Stocks
This publication contains information about 18 stocks:
Stock NameUniquenameType
BGBGcultivar
CNJ02-1-159CNJ02-1-159breeding_research_material
CNJ02-1-160CNJ02-1-160breeding_research_material
CNJ02-1-161CNJ02-1-161breeding_research_material
CNJ02-1-162CNJ02-1-162breeding_research_material
CNJ02-1-163CNJ02-1-163breeding_research_material
CNJ02-1-164CNJ02-1-164breeding_research_material
CNJ02-1-165CNJ02-1-165breeding_research_material
CNJ02-1-166CNJ02-1-166breeding_research_material
CNJ02-1-168CNJ02-1-168breeding_research_material
CNJ02-1-169CNJ02-1-169breeding_research_material
CNJ02-1-170CNJ02-1-170breeding_research_material
CNJ02-1-173CNJ02-1-173breeding_research_material
CNJ02-1-174CNJ02-1-174breeding_research_material
CNJ02-1-175CNJ02-1-175breeding_research_material
CNJ02-1-176CNJ02-1-176breeding_research_material
CNJ02-1-178CNJ02-1-178breeding_research_material
LoRedLoRedcultivar
Features
This publication contains information about 1,394 features:
Feature NameUniquenameType
KP278687KP278687.1region
KP278686KP278686.1region
KP278685KP278685.1region
KP278684KP278684.1region
KP278683KP278683.1region
KP278682KP278682.1region
KP278681KP278681.1region
KP278680KP278680.1region
KP278679KP278679.1region
KP278678KP278678.1region
KP278677KP278677.1region
KP278676KP278676.1region
KP278675KP278675.1region
KP278674KP278674.1region
KP278673KP278673.1region
KP278672KP278672.1region
KP278671KP278671.1region
KP278670KP278670.1region
KP278669KP278669.1region
KP278668KP278668.1region
KP278667KP278667.1region
KP278666KP278666.1region
KP278665KP278665.1region
KP278664KP278664.1region
KP278663KP278663.1region

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic
ISSN1420-3049
eISSN1420-3049
Publication Date2015
Journal AbbreviationMolecules
DOI10.3390/molecules20022001
Elocation10.3390/molecules20022001
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland
Publication TypeResearch Support, Non-U.S. Gov't
Publication TypeResearch Support, U.S. Gov't, Non-P.H.S.
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:25633331
AGL: USDA National Agricultural LibraryAGL:ns7432