Development and validation of 697 novel polymorphic genomic and EST-SSR markers in the American cranberry (Vaccinium macrocarpon Ait.)

Publication Overview
TitleDevelopment and validation of 697 novel polymorphic genomic and EST-SSR markers in the American cranberry (Vaccinium macrocarpon Ait.)
AuthorsSchlautman B, Fajardo D, Bougie T, Wiesman E, Polashock J, Vorsa N, Steffan S, Zalapa J
TypeJournal Article
Journal NameMolecules (Basel, Switzerland)
Volume20
Issue2
Year2015
Page(s)2001-13
CitationSchlautman B, Fajardo D, Bougie T, Wiesman E, Polashock J, Vorsa N, Steffan S, Zalapa J. Development and validation of 697 novel polymorphic genomic and EST-SSR markers in the American cranberry (Vaccinium macrocarpon Ait.). Molecules (Basel, Switzerland). 2015; 20(2):2001-13.

Abstract

The American cranberry, Vaccinium macrocarpon Ait., is an economically important North American fruit crop that is consumed because of its unique flavor and potential health benefits. However, a lack of abundant, genome-wide molecular markers has limited the adoption of modern molecular assisted selection approaches in cranberry breeding programs. To increase the number of available markers in the species, this study identified, tested, and validated microsatellite markers from existing nuclear and transcriptome sequencing data. In total, new primers were designed, synthesized, and tested for 979 SSR loci; 697 of the markers amplified allele patterns consistent with single locus segregation in a diploid organism and were considered polymorphic. Of the 697 polymorphic loci, 507 were selected for additional genetic diversity and segregation analyses in 29 cranberry genotypes. More than 95% of the 507 loci did not display segregation distortion at the p < 0.05 level, and contained moderate to high levels of polymorphism with a polymorphic information content >0.25. This comprehensive collection of developed and validated microsatellite loci represents a substantial addition to the molecular tools available for geneticists, genomicists, and breeders in cranberry and Vaccinium.

Stocks
This publication contains information about 18 stocks:
Stock NameUniquenameType
BGBGcultivar
CNJ02-1-159CNJ02-1-159breeding_research_material
CNJ02-1-160CNJ02-1-160breeding_research_material
CNJ02-1-161CNJ02-1-161breeding_research_material
CNJ02-1-162CNJ02-1-162breeding_research_material
CNJ02-1-163CNJ02-1-163breeding_research_material
CNJ02-1-164CNJ02-1-164breeding_research_material
CNJ02-1-165CNJ02-1-165breeding_research_material
CNJ02-1-166CNJ02-1-166breeding_research_material
CNJ02-1-168CNJ02-1-168breeding_research_material
CNJ02-1-169CNJ02-1-169breeding_research_material
CNJ02-1-170CNJ02-1-170breeding_research_material
CNJ02-1-173CNJ02-1-173breeding_research_material
CNJ02-1-174CNJ02-1-174breeding_research_material
CNJ02-1-175CNJ02-1-175breeding_research_material
CNJ02-1-176CNJ02-1-176breeding_research_material
CNJ02-1-178CNJ02-1-178breeding_research_material
LoRedLoRedcultivar
Features
This publication contains information about 1,394 features:
Feature NameUniquenameType
ct89379ct89379genetic_marker
ct89569ct89569genetic_marker
ct89711ct89711genetic_marker
ct92708ct92708genetic_marker
ct93137ct93137genetic_marker
ct94504ct94504genetic_marker
ct95345ct95345genetic_marker
ct95842ct95842genetic_marker
ct97791ct97791genetic_marker
ct98042ct98042genetic_marker
SCF1001SCF1001genetic_marker
SCF100820SCF100820genetic_marker
SCF101064SCF101064genetic_marker
SCF101363SCF101363genetic_marker
SCF101878SCF101878genetic_marker
SCF101914SCF101914genetic_marker
SCF102190SCF102190genetic_marker
SCF102347SCF102347genetic_marker
SCF102509SCF102509genetic_marker
SCF102538SCF102538genetic_marker
SCF10459SCF10459genetic_marker
SCF104688SCF104688genetic_marker
SCF1047SCF1047genetic_marker
SCF105092SCF105092genetic_marker
SCF10514SCF10514genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic
ISSN1420-3049
eISSN1420-3049
Publication Date2015
Journal AbbreviationMolecules
DOI10.3390/molecules20022001
Elocation10.3390/molecules20022001
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland
Publication TypeResearch Support, Non-U.S. Gov't
Publication TypeResearch Support, U.S. Gov't, Non-P.H.S.
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:25633331
AGL: USDA National Agricultural LibraryAGL:ns7432