Cranberry microsatellite marker development from assembled next-generation genomic sequence

Publication Overview
TitleCranberry microsatellite marker development from assembled next-generation genomic sequence
AuthorsGeorgi L, Herai RH, Vidal R, Falsarella Carazzolle M, Guimarães Pereira G, Polashock J, Vorsa N
TypeJournal Article
Journal NameMolecular breeding
Year2011
CitationGeorgi L, Herai RH, Vidal R, Falsarella Carazzolle M, Guimarães Pereira G, Polashock J, Vorsa N. Cranberry microsatellite marker development from assembled next-generation genomic sequence. Molecular breeding. 2011.

Abstract

The large-fruited cranberry (Vaccinium macrocarpon Ait.) is a native North American fruit that is a rich source of dietary phytochemicals with demonstrated and potential benefits for human health. Cranberry is a perennial, self-fertile 2n=2x=24 diploid, with a haploid genome size about 570 Mbp. Present commercial cultivars are only a few breeding and selection cycles removed from their wild progenitors. Resources, such as transcript or genomic sequences, molecular genetic markers, and genetic linkage maps, are needed to facilitate genetic enhancement. We have begun to generate these resources, starting with next-generation (SOLiD mate-paired) sequencing of an inbred cranberry clone, assembling the reads, and developing microsatellite markers from the assembled sequence. Development and testing of cranberry genomic microsatellite primers allows 1) testing the accuracy of the sequence assembly, 2) acquiring much-needed molecular markers for a genetic linkage map of cranberry and 3) permitting sequence scaffolds to be anchored on the genetic map.
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
CNJ99-125-1_inbred_F5CNJ99-125-1_inbred_F5accession
Features
This publication contains information about 48 features:
Feature NameUniquenameType
scf10kscf10kgenetic_marker
scf11iscf11igenetic_marker
scf12iscf12igenetic_marker
scf13ascf13agenetic_marker
scf14jscf14jgenetic_marker
scf15903cscf15903cgenetic_marker
scf15ascf15agenetic_marker
scf16iscf16igenetic_marker
scf17kscf17kgenetic_marker
scf18escf18egenetic_marker
scf19xscf19xgenetic_marker
scf1hscf1hgenetic_marker
scf20oscf20ogenetic_marker
scf21gscf21ggenetic_marker
scf22mscf22mgenetic_marker
scf23dscf23dgenetic_marker
scf24kscf24kgenetic_marker
scf252gscf252ggenetic_marker
scf25mscf25mgenetic_marker
scf26rscf26rgenetic_marker
scf27lscf27lgenetic_marker
scf28bscf28bgenetic_marker
scf29jscf29jgenetic_marker
scf2sscf2sgenetic_marker
scf30gscf30ggenetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication Date2011
Published Location|||
Language Abbreng
Publication Model[electronic resource].
Publication TypeJournal Article
URLhttp://dx.doi.org/10.1007/s11032-011-9613-7
KeywordsDNA primers, Vaccinium macrocarpon, breeding, chromosome mapping, cultivars, genetic markers, genomics, inbred lines, messenger RNA, microsatellite repeats
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:ns4131