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Protein to Nucleotide BLAST (tblastn)
Request a New BLAST
Enter
Protein
Query Sequence
Enter one or more queries in the top text box or use the browse button to upload a file from your local disk. The file may contain a single sequence or a list of sequences. In both cases, the data must be in
FASTA format
.
Show an Example Sequence
Enter FASTA sequence(s)
Or upload your own query FASTA:
Upload
The file should be a plain-text FASTA (.fasta, .fna, .fa, .fas) file. In other words, it cannot have formatting as is the case with MS Word (.doc, .docx) or Rich Text Format (.rtf). It cannot be greater than
2048MB
in size.
Don't forget to press the Upload button before attempting to submit your BLAST.
Choose Search Target
Choose from one of the
nucleotide
BLAST databases listed below.
Nucleotide
BLAST Databases:
V. corymbosum NCBI ESTs
V. corymbosum genome (Aug 2015) scaffolds
V. corymbosum unigene v1.0 contigs
V. corymbosum GDV RefTrans v1
V. corymbosum cv. Draper v1.0 genome scaffolds
V. corymbosum cv. Draper v1.0 genome transcripts
V. corymbosum genome (May 2013) cDNA
V. corymbosum genome (May 2013) scaffolds
V. macrocarpon cv. Stevens v1.0 genome scaffolds
V. macrocarpon genome WGS scaffolds (NCBI)
V. macrocarpon transcriptome (NCBI)
V. microcarpum v1.0 genome scaffolds
V. virgatum transcriptome
Select a Dataset
Advanced Options
General parameters
Max target sequences:
10
50
100
250
500
1000
5000
10000
20000
e-Value (Expected Threshold)
Expected number of chance matches in a random model. This number should be give in a decimal format.
More Information
|
Expect value vedio tutorial
Word size:
3
6
Max matches in a query range
Scoring parameters
Matrix
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Gap Costs:
Existence: 7 Extension: 2
Existence: 6 Extension: 2
Existence: 5 Extension: 2
Existence: 10 Extension: 1
Existence: 9 Extension: 1
Existence: 8 Extension: 1
BLAST