Haplotype-phased genome and evolution of phytonutrient pathways of tetraploid blueberry

Publication Overview
TitleHaplotype-phased genome and evolution of phytonutrient pathways of tetraploid blueberry
AuthorsColle M, Leisner CP, Wai CM, Ou S, Bird KA, Wang J, Wisecaver JH, Yocca AE, Alger EI, Tang H, Xiong Z, Callow P, Ben-Zvi G, Brodt A, Baruch K, Swale T, Shiue L, Song GQ, Childs KL, Schilmiller A, Vorsa N, Buell CR, VanBuren R, Jiang N, Edger PP
TypeJournal Article
Journal NameGigaScience
Year2019
CitationColle M, Leisner CP, Wai CM, Ou S, Bird KA, Wang J, Wisecaver JH, Yocca AE, Alger EI, Tang H, Xiong Z, Callow P, Ben-Zvi G, Brodt A, Baruch K, Swale T, Shiue L, Song GQ, Childs KL, Schilmiller A, Vorsa N, Buell CR, VanBuren R, Jiang N, Edger PP. Haplotype-phased genome and evolution of phytonutrient pathways of tetraploid blueberry. GigaScience. 2019 Jan 31.

Abstract

Background
Highbush blueberry (Vaccinium corymbosum) has long been consumed for its unique flavor and composition of health-promoting phytonutrients. However, breeding efforts to improve fruit quality in blueberry have been greatly hampered by the lack of adequate genomic resources and a limited understanding of the underlying genetics encoding key traits. The genome of highbush blueberry has been particularly challenging to assemble in large part to its polyploid nature and genome size.

Findings
Here, we present a chromosome-scale and haplotype-phased genome assembly of the cultivar "Draper," which has the highest antioxidant levels among a diversity panel of seventy-one cultivars and thirteen wild Vaccinium species. We leveraged this genome, combined with gene expression and metabolite data measured across fruit development, to identify candidate genes involved in the biosynthesis of important phytonutrients among other metabolites associated with superior fruit quality. Genome-wide analyses revealed that both polyploidy and tandem gene duplications modified various pathways involved in the biosynthesis of key phytonutrients. Furthermore, gene expression analyses hint at the presence of a spatial-temporal specific dominantly expressed subgenome including during fruit development.

Conclusions
These findings and the reference genome will serve as a valuable resource to guide future genome-enabled breeding of important agronomic traits in highbush blueberry.

Properties
Additional details for this publication include:
Property NameValue
URLhttps://academic.oup.com/gigascience/article-lookup/doi/10.1093/gigascience/giz012
Publication TypeJournal Article
Publication ModelPrint-Electronic
Publication Date2019 Jan 31
Language Abbreng
LanguageEnglish
Journal CountryUnited States
Journal AbbreviationGigascience
ISSN2047-217X
Elocation10.1093/gigascience/giz012
eISSN2047-217X
DOI10.1093/gigascience/giz012
Cross References
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DatabaseAccession
PMID: PMID:30715294