High-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population.

Publication Overview
TitleHigh-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population.
AuthorsQi X, Ogden EL, Bostan H, Sargent DJ, Ward J, Gilbert J, Iorizzo M, Rowland LJ
TypeJournal Article
Journal NameFrontiers in plant science
Volume12
Year2021
Page(s)692628
CitationQi X, Ogden EL, Bostan H, Sargent DJ, Ward J, Gilbert J, Iorizzo M, Rowland LJ. High-Density Linkage Map Construction and QTL Identification in a Diploid Blueberry Mapping Population.. Frontiers in plant science. 2021; 12:692628.

Abstract

Genotyping by sequencing approaches have been widely applied in major crops and are now being used in horticultural crops like berries and fruit trees. As the original and largest producer of cultivated blueberry, the United States maintains the most diverse blueberry germplasm resources comprised of many species of different ploidy levels. We previously constructed an interspecific mapping population of diploid blueberry by crossing the parent F1#10 (Vaccinium darrowii Fla4B × diploid V. corymbosum W85-20) with the parent W85-23 (diploid V. corymbosum). Employing the Capture-Seq technology developed by RAPiD Genomics, with an emphasis on probes designed in predicted gene regions, 117 F1 progeny, the two parents, and two grandparents of this population were sequenced, yielding 131.7 Gbp clean sequenced reads. A total of 160,535 single nucleotide polymorphisms (SNPs), referenced to 4,522 blueberry genome sequence scaffolds, were identified and subjected to a parent-dependent sliding window approach to further genotype the population. Recombination breakpoints were determined and marker bins were deduced to construct a high density linkage map. Twelve blueberry linkage groups (LGs) consisting of 17,486 SNP markers were obtained, spanning a total genetic distance of 1,539.4 cM. Among 18 horticultural traits phenotyped in this population, quantitative trait loci (QTLs) that were significant over at least 2 years were identified for chilling requirement, cold hardiness, and fruit quality traits of color, scar size, and firmness. Interestingly, in 1 year, a QTL associated with timing of early bloom, full bloom, petal fall, and early green fruit was identified in the same region harboring the major QTL for chilling requirement. In summary, we report here the first high density bin map of a diploid blueberry mapping population and the identification of several horticulturally important QTLs.

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Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Blueberry-Fruit_quality_chilling-Qi-2021
Featuremaps
This publication contains information about 1 maps:
Map Name
Blueberry-F1#10_x_W85-23-F1-2021
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
ISSN1664-462X
pISSN1664-462X
Publication Date2021
Journal AbbreviationFront Plant Sci
DOI10.3389/fpls.2021.692628
Elocation10.3389/fpls.2021.692628
CopyrightCopyright © 2021 Qi, Ogden, Bostan, Sargent, Ward, Gilbert, Iorizzo and Rowland.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland