GDV marker alignment to genome using primer sequences

Markers with primers in GDV were aligned to whole genome sequences using blastn-short. One primer set per marker was used in the blast and the best hits (over e-value 0.05) of each primer was used to calculate the aligned location for the markers. The product size cutoff was 100-500 bp for SSRs and 100-1500 bp for other markers such as EST, EST-SSR, gene marker, SCAR.