CV190401

EST Overview
NameCV190401
Unique NameCV190401
TypeEST
OrganismVaccinium corymbosum (northern highbush blueberry)
Sequence length183
Libraries
Library NameType
LIBEST_016374 Vco01cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Vaccinium corymbosum unigene v1EST
Homology
[Close]
BLAST of CV190401 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0004s13920.1 ()

HSP 1 Score: 96.6709 bits (239), Expect = 3.717e-21
Identity = 44/53 (83.02%), Postives = 51/53 (96.23%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDP+YS+E+MKVL H+K LPKVEGLVPIYISP+SGEFSGENIRLGSRGDSY
Sbjct:  315 VSGDPRYSIEAMKVLEHIKDLPKVEGLVPIYISPDSGEFSGENIRLGSRGDSY 367
[Close]
BLAST of CV190401 vs. P. persica 1.0 peptide
Match: ppa002795m ()

HSP 1 Score: 96.6709 bits (239), Expect = 2.523e-21
Identity = 44/53 (83.02%), Postives = 52/53 (98.11%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKYSVE+MKVL H+KTLPKVEGLVPI+I+P++GEFSGENIRLGSRGDSY
Sbjct:  315 VSGDPKYSVEAMKVLEHMKTLPKVEGLVPIFINPSTGEFSGENIRLGSRGDSY 367
[Close]
BLAST of CV190401 vs. TrEMBL
Match: D7TK48|D7TK48_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_3.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021446001 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.190e-19
Identity = 46/53 (86.79%), Postives = 51/53 (96.23%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKY VE+MKVL HLKTLPKVEGLVPIYISP+SGEF+GENIRLGSRGDSY
Sbjct:  128 VSGDPKYGVEAMKVLEHLKTLPKVEGLVPIYISPHSGEFNGENIRLGSRGDSY 180
[Close]
BLAST of CV190401 vs. TrEMBL
Match: B9S125|B9S125_RICCO (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative OS=Ricinus communis GN=RCOM_0632950 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.373e-19
Identity = 44/53 (83.02%), Postives = 51/53 (96.23%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKYS+E+MKVL H+K LPKVEGLVPIYISP++GEFSGENIRLGSRGDSY
Sbjct:  306 VSGDPKYSMEAMKVLAHMKNLPKVEGLVPIYISPDTGEFSGENIRLGSRGDSY 358
[Close]
BLAST of CV190401 vs. TrEMBL
Match: B9H405|B9H405_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831216 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.323e-19
Identity = 44/53 (83.02%), Postives = 51/53 (96.23%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDP+YS+E+MKVL H+K LPKVEGLVPIYISP+SGEFSGENIRLGSRGDSY
Sbjct:  315 VSGDPRYSIEAMKVLEHIKDLPKVEGLVPIYISPDSGEFSGENIRLGSRGDSY 367
[Close]
BLAST of CV190401 vs. TrEMBL
Match: Q9C8R9|Q9C8R9_ARATH (Endoplasmic reticulum alpha-mannosidase, putative; 33510-31408 OS=Arabidopsis thaliana GN=T1P2.10 PE=4 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 7.046e-18
Identity = 42/53 (79.25%), Postives = 48/53 (90.57%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKYS E+MKVL H+KTLPK EGLVPIYISP +G+F GENIRLGSRGDSY
Sbjct:  244 ISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGENIRLGSRGDSY 296
[Close]
BLAST of CV190401 vs. TrEMBL
Match: Q93Y37|Q93Y37_ARATH (Endoplasmic reticulum alpha-mannosidase, putative OS=Arabidopsis thaliana GN=At1g30000/ T1P2.10 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 7.046e-18
Identity = 42/53 (79.25%), Postives = 48/53 (90.57%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKYS E+MKVL H+KTLPK EGLVPIYISP +G+F GENIRLGSRGDSY
Sbjct:  307 ISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGENIRLGSRGDSY 359
[Close]
BLAST of CV190401 vs. TrEMBL
Match: Q75IK7|Q75IK7_ORYSJ (Putative alpha-mannosidase OS=Oryza sativa subsp. japonica GN=OSJNBb0016G07.5 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.570e-17
Identity = 41/53 (77.36%), Postives = 50/53 (94.34%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKY +E+MKVL H++TLP VEGLVPIYI+P+SG+FSGENIRLGSRGDSY
Sbjct:  377 ISGDPKYDLEAMKVLEHMRTLPTVEGLVPIYINPSSGQFSGENIRLGSRGDSY 429
[Close]
BLAST of CV190401 vs. TrEMBL
Match: Q0DK00|Q0DK00_ORYSJ (Os05g0209100 protein OS=Oryza sativa subsp. japonica GN=Os05g0209100 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.570e-17
Identity = 41/53 (77.36%), Postives = 50/53 (94.34%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKY +E+MKVL H++TLP VEGLVPIYI+P+SG+FSGENIRLGSRGDSY
Sbjct:  321 ISGDPKYDLEAMKVLEHMRTLPTVEGLVPIYINPSSGQFSGENIRLGSRGDSY 373
[Close]
BLAST of CV190401 vs. TrEMBL
Match: A2Y1I7|A2Y1I7_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_18866 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.570e-17
Identity = 41/53 (77.36%), Postives = 50/53 (94.34%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKY +E+MKVL H++TLP VEGLVPIYI+P+SG+FSGENIRLGSRGDSY
Sbjct:  377 ISGDPKYDLEAMKVLEHMRTLPTVEGLVPIYINPSSGQFSGENIRLGSRGDSY 429
[Close]
BLAST of CV190401 vs. TrEMBL
Match: D7KEP5|D7KEP5_ARALY (Glycoside hydrolase family 47 protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_890537 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.677e-17
Identity = 41/53 (77.36%), Postives = 47/53 (88.68%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKYS E+MKVL H+K LPK EGLVPIYISP +G+F GENIRLGSRGDSY
Sbjct:  307 ISGDPKYSTEAMKVLAHIKNLPKTEGLVPIYISPQTGDFVGENIRLGSRGDSY 359
[Close]
BLAST of CV190401 vs. TrEMBL
Match: C5YUN6|C5YUN6_SORBI (Putative uncharacterized protein Sb09g006500 OS=Sorghum bicolor GN=Sb09g006500 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.677e-17
Identity = 42/53 (79.25%), Postives = 49/53 (92.45%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKY  E+MKVL H+ TLPKVEGLVPIYI+P+SG+FSGENIRLGSRGDSY
Sbjct:  318 VSGDPKYDREAMKVLEHMHTLPKVEGLVPIYINPSSGQFSGENIRLGSRGDSY 370
[Close]
BLAST of CV190401 vs. Vitis vinifera peptide
Match: GSVIVT01021446001 (assembled CDS)

HSP 1 Score: 98.5969 bits (244), Expect = 5.690e-22
Identity = 46/53 (86.79%), Postives = 51/53 (96.23%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKY VE+MKVL HLKTLPKVEGLVPIYISP+SGEF+GENIRLGSRGDSY
Sbjct:  128 VSGDPKYGVEAMKVLEHLKTLPKVEGLVPIYISPHSGEFNGENIRLGSRGDSY 180
[Close]
BLAST of CV190401 vs. TAIR peptide
Match: AT1G30000.1 (| Symbols: MNS3 | alpha-mannosidase 3 | chr1:10509532-10512320 REVERSE LENGTH=624)

HSP 1 Score: 93.5893 bits (231), Expect = 2.681e-20
Identity = 42/53 (79.25%), Postives = 48/53 (90.57%), Query Frame = 2 
Query:   23 LTGDPKYSVESMKVLGHLKTLPKVEGLVPIYISPNSGEFSGENIRLGSRGDSY 181
            ++GDPKYS E+MKVL H+KTLPK EGLVPIYISP +G+F GENIRLGSRGDSY
Sbjct:  307 ISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGENIRLGSRGDSY 359
The following BLAST results are available for this feature:
BLAST of CV190401 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
POPTR_0004s13920.13.717e-2183.02[more]
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BLAST of CV190401 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
ppa002795m2.523e-2183.02[more]
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BLAST of CV190401 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10
Match NameE-valueIdentityDescription
D7TK48|D7TK48_VITVI2.190e-1986.79Whole genome shotgun sequence of line PN40024, sca... [more]
B9S125|B9S125_RICCO6.373e-1983.02Endoplasmic reticulum mannosyl-oligosaccharide 1,2... [more]
B9H405|B9H405_POPTR8.323e-1983.02Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
Q9C8R9|Q9C8R9_ARATH7.046e-1879.25Endoplasmic reticulum alpha-mannosidase, putative;... [more]
Q93Y37|Q93Y37_ARATH7.046e-1879.25Endoplasmic reticulum alpha-mannosidase, putative ... [more]
Q75IK7|Q75IK7_ORYSJ1.570e-1777.36Putative alpha-mannosidase OS=Oryza sativa subsp. ... [more]
Q0DK00|Q0DK00_ORYSJ1.570e-1777.36Os05g0209100 protein OS=Oryza sativa subsp. japoni... [more]
A2Y1I7|A2Y1I7_ORYSI1.570e-1777.36Putative uncharacterized protein OS=Oryza sativa s... [more]
D7KEP5|D7KEP5_ARALY2.677e-1777.36Glycoside hydrolase family 47 protein OS=Arabidops... [more]
C5YUN6|C5YUN6_SORBI2.677e-1779.25Putative uncharacterized protein Sb09g006500 OS=So... [more]
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BLAST of CV190401 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1
Match NameE-valueIdentityDescription
GSVIVT010214460015.690e-2286.79assembled CDS[more]
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BLAST of CV190401 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT1G30000.12.681e-2079.25| Symbols: MNS3 | alpha-mannosidase 3 | chr1:10509... [more]
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InterPro
Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001382Glycoside hydrolase, family 47GENE3D1.50.10.50no descriptioncoord: 3..55
score: 3.9
IPR001382Glycoside hydrolase, family 47PANTHERPTHR11742MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATEDcoord: 3..55
score: 1.5
IPR001382Glycoside hydrolase, family 47PFAMPF01532Glyco_hydro_47coord: 2..55
score: 6.7
IPR001382Glycoside hydrolase, family 47SUPERFAMILY48225Seven-hairpin glycosidasescoord: 3..55
score: 1.9
NoneNo IPR availablePANTHERPTHR11742:SF7ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASEcoord: 3..55
score: 1.5

Properties
Property NameValue
Singlet in analysisVaccinium corymbosum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>CV190401 ID=CV190401; Name=CV190401; organism=Vaccinium corymbosum; type=EST; length=183bp
GGGTTCAATTATCTAAGCTCCNCTCACTGGTGATCCAAAGTATAGTGTGG
AATCCATGAAGGTCTTGGGACATTTGAAGACTCTCCCAAAAGTGGAAGGG
CTGGTTCCTATTTACATAAGCCCTAACAGTGGTGAATTTAGTGGTGAGAA
CATTCGACTGGGATCTCGTGGTGACAGCTACTA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: KEGG ORTHOLOGY
TermDefinition
K01230MAN1; mannosyl-oligosaccharide alpha-1,2-mannosidase [EC:3.2.1.113]
Vocabulary: INTERPRO
TermDefinition
IPR001382Glyco_hydro_47
noIPRunintegrated