CF811031 | GDV

EST Overview


Name	CF811031
Unique Name	CF811031
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	643

Libraries


Library Name	Type
LIBEST_014530 cDNA cold acclimated Bluecrop library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of CF811031 vs. DB:swissprot:display
Match: RH7_ARATH (DEAD-box ATP-dependent RNA helicase 7 OS=Arabidopsis thaliana GN=RH7 PE=1 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 9.538e-11
Identity = 33/53 (62.26%), Postives = 44/53 (83.02%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAG-QKNAGGVSLEVVKELPQLQERE 637
            E+++G++LTAD  GAVFDV   +LD F+AG QK+AG +SLEVVK +P+LQERE
Sbjct:  577 EMIEGLSLTADKTGAVFDVKQSDLDLFIAGAQKSAGSMSLEVVKVMPKLQERE 629

[Close]

BLAST of CF811031 vs. DB:swissprot:display
Match: RH7_SPIOL (DEAD-box ATP-dependent RNA helicase 7 OS=Spinacia oleracea GN=RH7 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.246e-10
Identity = 30/49 (61.22%), Postives = 41/49 (83.67%), Query Frame = -1

Query:  485 VKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQER 631
            + G+ALTAD +GAVFDVP ++L+TFL G +NA GV+L+VVK LP L+E+
Sbjct:  590 IMGVALTADKSGAVFDVPVDDLETFLVGAENAAGVNLDVVKALPPLEEK 638

[Close]

BLAST of CF811031 vs. DB:swissprot:display
Match: RH7_ORYSJ (DEAD-box ATP-dependent RNA helicase 7 OS=Oryza sativa subsp. japonica GN=Os09g0520700 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 8.074e-10
Identity = 29/50 (58.00%), Postives = 40/50 (80.00%), Query Frame = -1

Query:  482 VKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 631
            VKG+ +TADG GAVFDVP+ E++ ++ G +NA  V++E VK+LP LQERE
Sbjct:  599 VKGITITADGTGAVFDVPSAEVEDYIQGAQNAAMVTVEEVKQLPPLQERE 648

[Close]

BLAST of CF811031 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0012s14190.1 ()

HSP 1 Score: 76.6406 bits (187), Expect = 1.402e-14
Identity = 37/52 (71.15%), Postives = 44/52 (84.62%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 637
            E V GM+LTADGNGAVFDV  E++DTFLA Q+NA GV++EVVK LP LQER+
Sbjct:  579 ESVTGMSLTADGNGAVFDVKKEDVDTFLAAQENAAGVNIEVVKVLPSLQERD 630

[Close]

BLAST of CF811031 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0015s14150.1 ()

HSP 1 Score: 72.7886 bits (177), Expect = 2.024e-13
Identity = 35/52 (67.31%), Postives = 40/52 (76.92%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 637
            E V GM LT DGNGAVFDV  E++D FLA Q+N  GV++EVVK LP LQERE
Sbjct:  583 ESVTGMTLTTDGNGAVFDVKKEDVDAFLAAQENGAGVNIEVVKTLPSLQERE 634

[Close]

BLAST of CF811031 vs. P. persica 1.0 peptide
Match: ppa002304m ()

HSP 1 Score: 79.337 bits (194), Expect = 1.529e-15
Identity = 39/53 (73.58%), Postives = 45/53 (84.91%), Query Frame = -1

Query:  479 ELVKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQEREA 637
            E VKGMALTADG GAVFDV AE+LD FLAGQ+NA  VS+EV++ LP LQE+EA
Sbjct:  592 ESVKGMALTADGKGAVFDVAAEDLDMFLAGQENAASVSIEVLESLPNLQEKEA 644

[Close]

BLAST of CF811031 vs. TrEMBL
Match: Q9M6R6|Q9M6R6_PHAAU (RNA helicase OS=Phaseolus aureus GN=VRH1 PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 1.205e-13
Identity = 37/52 (71.15%), Postives = 48/52 (92.31%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 637
            E VKG++LTADGNGAVFDVPAE+L+T+L+GQ+NA  VSLEV+K LP+LQ+R+
Sbjct:  610 EAVKGLSLTADGNGAVFDVPAEDLNTYLSGQENAANVSLEVLKALPRLQQRD 661

[Close]

BLAST of CF811031 vs. TrEMBL
Match: D7T1V8|D7T1V8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_22.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018476001 PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.579e-13
Identity = 37/50 (74.00%), Postives = 44/50 (88.00%), Query Frame = -1

Query:  482 VKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 631
            +KG+ALTADGNGAVFDV  E+LD FLAGQ+NA  VSLEV+K LP+LQER+
Sbjct:  455 IKGLALTADGNGAVFDVATEDLDAFLAGQENAANVSLEVLKALPRLQERD 504

[Close]

BLAST of CF811031 vs. TrEMBL
Match: B9I4I4|B9I4I4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_806661 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.332e-12
Identity = 37/52 (71.15%), Postives = 44/52 (84.62%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 637
            E V GM+LTADGNGAVFDV  E++DTFLA Q+NA GV++EVVK LP LQER+
Sbjct:  544 ESVTGMSLTADGNGAVFDVKKEDVDTFLAAQENAAGVNIEVVKALPSLQERD 595

[Close]

BLAST of CF811031 vs. TrEMBL
Match: B9R9L5|B9R9L5_RICCO (Dead box ATP-dependent RNA helicase, putative OS=Ricinus communis GN=RCOM_1498590 PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 6.612e-12
Identity = 36/52 (69.23%), Postives = 42/52 (80.77%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 637
            E VKGM LTADG GAVFDV A ++DTFLAGQ NA  VSLE++K LP LQE++
Sbjct:  586 ESVKGMTLTADGKGAVFDVAAADIDTFLAGQDNAANVSLEILKALPPLQEKD 637

[Close]

BLAST of CF811031 vs. TrEMBL
Match: B9ICY7|B9ICY7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_824889 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.513e-11
Identity = 35/52 (67.31%), Postives = 40/52 (76.92%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 637
            E V GM LT DGNGAVFDV  E++D FLA Q+N  GV++EVVK LP LQERE
Sbjct:  560 ESVTGMTLTTDGNGAVFDVKKEDVDAFLAAQENGAGVNIEVVKTLPSLQERE 611

[Close]

BLAST of CF811031 vs. TrEMBL
Match: Q6RFT2|Q6RFT2_CAPAN (RNA helicase PRH75 OS=Capsicum annuum PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.282e-11
Identity = 36/52 (69.23%), Postives = 40/52 (76.92%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 637
            E + G+ LTADG GAVFDV A+ LD FLAGQK A GV LEVV+ LP LQERE
Sbjct:  130 ESINGLTLTADGKGAVFDVSADHLDEFLAGQKTAHGVKLEVVEALPPLQERE 181

[Close]

BLAST of CF811031 vs. TrEMBL
Match: D7MLB4|D7MLB4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_496396 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.352e-9
Identity = 34/53 (64.15%), Postives = 44/53 (83.02%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLA-GQKNAGGVSLEVVKELPQLQERE 637
            E ++G++LTAD +GAVFDV   +L  FLA GQK+AG +SLEVVKE+P+LQERE
Sbjct:  577 EQIEGLSLTADKSGAVFDVKQSDLALFLAAGQKSAGSMSLEVVKEMPKLQERE 629

[Close]

BLAST of CF811031 vs. TrEMBL
Match: A6MCY9|A6MCY9_9ORYZ (DEAD/DEAH box helicase family protein OS=Oryza brachyantha PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 8.937e-9
Identity = 29/50 (58.00%), Postives = 40/50 (80.00%), Query Frame = -1

Query:  482 VKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 631
            VKG+ LTADG GAVFDVP+ E++ ++ G +NA  V++E VK+LP LQER+
Sbjct:  589 VKGITLTADGTGAVFDVPSAEVEDYIQGSENAAMVTIEEVKQLPPLQERD 638

[Close]

BLAST of CF811031 vs. TrEMBL
Match: A3C0Q9|A3C0Q9_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_30047 PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.167e-8
Identity = 29/50 (58.00%), Postives = 40/50 (80.00%), Query Frame = -1

Query:  482 VKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 631
            VKG+ +TADG GAVFDVP+ E++ ++ G +NA  V++E VK+LP LQERE
Sbjct:  588 VKGITITADGTGAVFDVPSAEVEDYIQGAQNAAMVTVEEVKQLPPLQERE 637

[Close]

BLAST of CF811031 vs. TrEMBL
Match: A2Z376|A2Z376_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_32075 PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.167e-8
Identity = 29/50 (58.00%), Postives = 40/50 (80.00%), Query Frame = -1

Query:  482 VKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 631
            VKG+ +TADG GAVFDVP+ E++ ++ G +NA  V++E VK+LP LQERE
Sbjct:  588 VKGITITADGTGAVFDVPSAEVEDYIQGAQNAAMVTVEEVKQLPPLQERE 637

[Close]

BLAST of CF811031 vs. Vitis vinifera peptide
Match: GSVIVT01018476001 (assembled CDS)

HSP 1 Score: 78.5666 bits (192), Expect = 2.256e-15
Identity = 37/50 (74.00%), Postives = 44/50 (88.00%), Query Frame = -1

Query:  482 VKGMALTADGNGAVFDVPAEELDTFLAGQKNAGGVSLEVVKELPQLQERE 631
            +KG+ALTADGNGAVFDV  E+LD FLAGQ+NA  VSLEV+K LP+LQER+
Sbjct:  455 IKGLALTADGNGAVFDVATEDLDAFLAGQENAANVSLEVLKALPRLQERD 504

[Close]

BLAST of CF811031 vs. TAIR peptide
Match: AT5G62190.1 (| Symbols: PRH75 | DEAD box RNA helicase (PRH75) | chr5:24980542-24983879 REVERSE LENGTH=671)

HSP 1 Score: 67.0106 bits (162), Expect = 9.777e-12
Identity = 33/53 (62.26%), Postives = 44/53 (83.02%), Query Frame = -1

Query:  482 ELVKGMALTADGNGAVFDVPAEELDTFLAG-QKNAGGVSLEVVKELPQLQERE 637
            E+++G++LTAD  GAVFDV   +LD F+AG QK+AG +SLEVVK +P+LQERE
Sbjct:  577 EMIEGLSLTADKTGAVFDVKQSDLDLFIAGAQKSAGSMSLEVVKVMPKLQERE 629

The following BLAST results are available for this feature:

BLAST of CF811031 vs. DB:swissprot:display
BLAST of CF811031 vs. Populus trichocarpa peptide v2.0
BLAST of CF811031 vs. P. persica 1.0 peptide
BLAST of CF811031 vs. TrEMBL
BLAST of CF811031 vs. Vitis vinifera peptide
BLAST of CF811031 vs. TAIR peptide

BLAST of CF811031 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
RH7_ARATH	9.538e-11	62.26	DEAD-box ATP-dependent RNA helicase 7 OS=Arabidops...	[more]
RH7_SPIOL	1.246e-10	61.22	DEAD-box ATP-dependent RNA helicase 7 OS=Spinacia ...	[more]
RH7_ORYSJ	8.074e-10	58.00	DEAD-box ATP-dependent RNA helicase 7 OS=Oryza sat...	[more]

BLAST of CF811031 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
POPTR_0012s14190.1	1.402e-14	71.15		[more]
POPTR_0015s14150.1	2.024e-13	67.31		[more]

BLAST of CF811031 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ppa002304m	1.529e-15	73.58		[more]

BLAST of CF811031 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
Q9M6R6\|Q9M6R6_PHAAU	1.205e-13	71.15	RNA helicase OS=Phaseolus aureus GN=VRH1 PE=2 SV=1	[more]
D7T1V8\|D7T1V8_VITVI	4.579e-13	74.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9I4I4\|B9I4I4_POPTR	1.332e-12	71.15	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9R9L5\|B9R9L5_RICCO	6.612e-12	69.23	Dead box ATP-dependent RNA helicase, putative OS=R...	[more]
B9ICY7\|B9ICY7_POPTR	2.513e-11	67.31	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q6RFT2\|Q6RFT2_CAPAN	3.282e-11	69.23	RNA helicase PRH75 OS=Capsicum annuum PE=2 SV=1	[more]
D7MLB4\|D7MLB4_ARALY	2.352e-9	64.15	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
A6MCY9\|A6MCY9_9ORYZ	8.937e-9	58.00	DEAD/DEAH box helicase family protein OS=Oryza bra...	[more]
A3C0Q9\|A3C0Q9_ORYSJ	1.167e-8	58.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A2Z376\|A2Z376_ORYSI	1.167e-8	58.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of CF811031 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1


Match Name	E-value	Identity	Description
GSVIVT01018476001	2.256e-15	74.00	assembled CDS	[more]

BLAST of CF811031 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G62190.1	9.777e-12	62.26	\| Symbols: PRH75 \| DEAD box RNA helicase (PRH75) \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 56..69 score
None	No IPR available	SEG	seg	seg	coord: 26..61 score
None	No IPR available	SEG	seg	seg	coord: 58..108 score
None	No IPR available	SEG	seg	seg	coord: 16..46 score
None	No IPR available	SEG	seg	seg	coord: 55..102 score
IPR012562	GUCT	PFAM	PF08152	GUCT	coord: 4..51 score: 2.9

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF811031 ID=CF811031; Name=CF811031; organism=Vaccinium corymbosum; type=EST; length=643bp
GCATATAAAATCAATCTATTGATAAATTTGAATTCGGTACATTTATCGCA
TAAATAAGAGTACATTCTGCTCTGTAATCGTTTCCTGATTTGCAAAACTT
TAAGTGGAGAGGCAGGCCAAAAACCCCTCCAAAAAATGAGCAATGATGGA
CATAAATAATAGTGGCTACATTCCAATATTCAGCTTTTCTATTGTACGAC
AAATTAGCATCCGAATTACTACATCCCGATTTTATTTGTTGGTCACAAAA
ACTCTTCGTCGCAACAGGCAACCAAATGCTGTTTCAGTTAGCTGAATGTT
TTCTGAAAATCTCAGCCGCTCACCATTTGTTGTTACCGCGACCCCTCCCT
CCACCGAAGCCTCTGGAAAATCCACCACTTCTTCGGTCAGAGAAACCACC
ACCTCTTCCTCCAGAAAACCTACCACCACCGCCGCCTCTCCTATCACCAA
AACCACCACTGCCAAATCTTCCTCCTCTTGCTTCTCTCTCTTGCAACTGA
GGCAACTCTTTCACCACCTCTAAACTCACACCACCAGCATTTTTCTGGCC
AGCAAGAAATGTGTCCAACTCTTCAGCAGGCACATCAAATACTGCCCCAT
TTCCATCAGCTGTTAGAGCCATACCCTTAACCAGCTCCTCTTR

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0004386	helicase activity
GO:0005524	ATP binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **INTERPRO**
Term	Definition
noIPR	unintegrated
IPR012562	GUCT