HO054862

EST Overview
NameHO054862
Unique NameHO054862
TypeEST
OrganismVaccinium corymbosum (northern highbush blueberry)
Sequence length442
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Vaccinium corymbosum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Vaccinium corymbosum unigene v12011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL2011-01-18
InterProScan analysis for Vaccinium corymbosum unigene v12011-01-18
Homology
[Close]
BLAST of HO054862 vs. P. persica 1.0 peptide
Match: ppa000037m ()

HSP 1 Score: 50.8322 bits (120), Expect = 2.844e-7
Identity = 33/75 (44.00%), Postives = 42/75 (56.00%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G + +        S AG+GLISPPP  DI S+EDL Q  H LK  N  A  S+K++S+V    V  G
Sbjct: 1090 EGGELP--GHKVIGDIAVTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPTARISVKLVSEVGVGVVASG 1162
[Close]
BLAST of HO054862 vs. TrEMBL
Match: Q3JMX1|Q3JMX1_BURP1 (Glutamate synthase, large subunit OS=Burkholderia pseudomallei (strain 1710b) GN=gltB PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.606e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1089 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1161

HSP 2 Score: 20.7866 bits (42), Expect = 2.606e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct: 1066 VTAEYLASADQI 1077
[Close]
BLAST of HO054862 vs. TrEMBL
Match: Q2SU81|Q2SU81_BURTA (Glutamate synthase, large subunit OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=BTH_I3014 PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.607e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1065 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1137

HSP 2 Score: 20.7866 bits (42), Expect = 2.607e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct: 1042 VTAEYLASADQI 1053
[Close]
BLAST of HO054862 vs. TrEMBL
Match: Q62GB8|Q62GB8_BURMA (Glutamate synthase, large subunit OS=Burkholderia mallei GN=gltB PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.609e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1022 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1094

HSP 2 Score: 20.7866 bits (42), Expect = 2.609e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct:  999 VTAEYLASADQI 1010
[Close]
BLAST of HO054862 vs. TrEMBL
Match: Q63Q66|Q63Q66_BURPS (Glutamate synthase large subunit OS=Burkholderia pseudomallei GN=glt1 PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.610e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1008 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1080

HSP 2 Score: 20.7866 bits (42), Expect = 2.610e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct:  985 VTAEYLASADQI 996
[Close]
BLAST of HO054862 vs. TrEMBL
Match: A3P056|A3P056_BURP0 (Glutamate synthase, large subunit OS=Burkholderia pseudomallei (strain 1106a) GN=gltB PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.610e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1008 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1080

HSP 2 Score: 20.7866 bits (42), Expect = 2.610e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct:  985 VTAEYLASADQI 996
[Close]
BLAST of HO054862 vs. TrEMBL
Match: A3NEC3|A3NEC3_BURP6 (Glutamate synthase, large subunit OS=Burkholderia pseudomallei (strain 668) GN=gltB PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.610e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1008 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1080

HSP 2 Score: 20.7866 bits (42), Expect = 2.610e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct:  985 VTAEYLASADQI 996
[Close]
BLAST of HO054862 vs. TrEMBL
Match: A3MPX2|A3MPX2_BURM7 (Glutamate synthase, large subunit OS=Burkholderia mallei (strain NCTC 10247) GN=gltB PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.610e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1008 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1080

HSP 2 Score: 20.7866 bits (42), Expect = 2.610e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct:  985 VTAEYLASADQI 996
[Close]
BLAST of HO054862 vs. TrEMBL
Match: A2S727|A2S727_BURM9 (Glutamate synthase, large subunit OS=Burkholderia mallei (strain NCTC 10229) GN=gltB PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.610e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1008 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1080

HSP 2 Score: 20.7866 bits (42), Expect = 2.610e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct:  985 VTAEYLASADQI 996
[Close]
BLAST of HO054862 vs. TrEMBL
Match: A1V8E6|A1V8E6_BURMS (Glutamate synthase, large subunit OS=Burkholderia mallei (strain SAVP1) GN=gltB PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.610e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1008 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1080

HSP 2 Score: 20.7866 bits (42), Expect = 2.610e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct:  985 VTAEYLASADQI 996
[Close]
BLAST of HO054862 vs. TrEMBL
Match: C6TZW5|C6TZW5_BURPS (Glutamate synthase, large subunit OS=Burkholderia pseudomallei 1710a GN=gltB PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.610e-7
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 3 
Query:   93 DGGMLPDDGPQGVEMQKEAARSVAGIGLISPPPLLDINSMEDLKQENHYLKIVNVIAMNSLKVMSDVSDDTVGRG 317
            +GG LP  G +  E   +   SV G+GLISPPP  DI S+EDL Q  H LK VN +A  S+K++S+V   TV  G
Sbjct: 1008 EGGQLP--GHKVSEYIGKLRYSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPVASISVKLVSEVGVGTVAAG 1080

HSP 2 Score: 20.7866 bits (42), Expect = 2.610e-7
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   32 VTAQYLAQADLI 67
            VTA+YLA AD I
Sbjct:  985 VTAEYLASADQI 996
The following BLAST results are available for this feature:
BLAST of HO054862 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
ppa000037m2.844e-744.00[more]
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BLAST of HO054862 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10
Match NameE-valueIdentityDescription
Q3JMX1|Q3JMX1_BURP12.606e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
Q2SU81|Q2SU81_BURTA2.607e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
Q62GB8|Q62GB8_BURMA2.609e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
Q63Q66|Q63Q66_BURPS2.610e-748.00Glutamate synthase large subunit OS=Burkholderia p... [more]
A3P056|A3P056_BURP02.610e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
A3NEC3|A3NEC3_BURP62.610e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
A3MPX2|A3MPX2_BURM72.610e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
A2S727|A2S727_BURM92.610e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
A1V8E6|A1V8E6_BURMS2.610e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
C6TZW5|C6TZW5_BURPS2.610e-748.00Glutamate synthase, large subunit OS=Burkholderia ... [more]
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InterPro
Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 26..61
score
NoneNo IPR availablePANTHERPTHR11938FAD NADPH DEHYDROGENASE/OXIDOREDUCTASEcoord: 40..77
score: 0.0
NoneNo IPR availablePANTHERPTHR11938:SF1GLUTAMATE SYNTHASEcoord: 40..77
score: 0.0
NoneNo IPR availableSEGsegsegcoord: 35..54
score
NoneNo IPR availableSEGsegsegcoord: 63..82
score
IPR002932Glutamate synthase, central-CPFAMPF01645Glu_synthasecoord: 40..94
score: 4.6
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70no descriptioncoord: 21..88
score: 6.6

Properties
Property NameValue
Singlet in analysisVaccinium corymbosum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO054862 ID=HO054862; Name=HO054862; organism=Vaccinium corymbosum; type=EST; length=442bp
AGATCATGATGGTCAGGTCGGGCCGTGCGAAGTGACGGCCCAGTATCTCG
CGCAAGCCGACCTTATCCTTGAGGGATGGGCTGTGCGGCTGCGACGGCGG
AATGCTACCAGATGACGGACCCCAAGGTGTCGAAATGCAAAAGGAAGCTG
CGCGCTCGGTGGCGGGGATCGGCCTCATCTCGCCGCCGCCGCTCCTCGAC
ATCAATTCTATGGAGGATCTCAAGCAGGAGAACCACTATCTTAAGATCGT
CAACGTGATCGCGATGAACTCGCTGAAGGTCATGTCGGATGTGAGCGACG
ACACGGTGGGGCGCGGGCGGACTTGCTCGAATCGCTGATTTCCTAGGTGA
TCGCCGTCGAAAAGAGAGCCCGATGCGCTTCTCCGCTGTCTCCGGTTAAA
TTCGCGGTTCCACAACTGGGAGCTCGGCCTCCCCGAGACGCA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Biological Process
TermDefinition
GO:0006537glutamate biosynthetic process
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0015930glutamate synthase activity
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
noIPRunintegrated
IPR002932Glu_synthdom
IPR013785Aldolase_TIM