DR067399

EST Overview
NameDR067399
Unique NameDR067399
TypeEST
OrganismVaccinium corymbosum (northern highbush blueberry)
Sequence length405
Libraries
Library NameType
LIBEST_016374 Vco01cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Vaccinium corymbosum unigene v1EST
Homology
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYIC_HUMAN (Isoleucyl-tRNA synthetase, cytoplasmic OS=Homo sapiens GN=IARS PE=1 SV=2)

HSP 1 Score: 157.147 bits (396), Expect = 2.534e-38
Identity = 74/105 (70.48%), Postives = 89/105 (84.76%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVE 325
            YTL+VL+TALFG+P ++N+I NGLVLA DG+KMSKR KNYP P+ +I  YGADALRLY+INSPVVRAE LRFK++GV  V+ DV LPWYNAYRFL+QN  RL+ E
Sbjct:  569 YTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKE 673
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYIC_DICDI (Probable isoleucyl-tRNA synthetase, cytoplasmic OS=Dictyostelium discoideum GN=ileS PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 9.631e-38
Identity = 74/103 (71.84%), Postives = 87/103 (84.47%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLE 319
            YTL+VLSTALF KP ++NLI NGLVLA DGKKMSKRLKNYP PMEV++  GAD+LRLY+INSPVVRAE L+F++ GV  ++ DVFLPW+NAYRF VQN  R E
Sbjct:  573 YTLLVLSTALFDKPPFQNLIVNGLVLAADGKKMSKRLKNYPDPMEVVSKVGADSLRLYLINSPVVRAETLKFQEKGVQDMIKDVFLPWFNAYRFFVQNCLRFE 675
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYIC_CAEEL (Isoleucyl-tRNA synthetase, cytoplasmic OS=Caenorhabditis elegans GN=irs-1 PE=2 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 9.631e-38
Identity = 73/105 (69.52%), Postives = 86/105 (81.90%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVE 325
            YTL+VLSTALF KP ++NLICNGLVLA DG KMSK  KNYP PM ++N YGADALRLY+INSPVVR E LRF+++GV  ++ DVFLPW+NAYRF VQN +  E E
Sbjct:  570 YTLLVLSTALFNKPPFKNLICNGLVLASDGAKMSKSKKNYPDPMLIVNKYGADALRLYLINSPVVRGENLRFREEGVRDLLKDVFLPWFNAYRFFVQNVQAYEHE 674
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYIC_MOUSE (Isoleucyl-tRNA synthetase, cytoplasmic OS=Mus musculus GN=Iars PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 1.643e-37
Identity = 72/105 (68.57%), Postives = 88/105 (83.81%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVE 325
            YTL+VL+TALFG+P ++N+I NGL+LA DG+KMSKR KNYP P+ +I+ YGADALRLY+INSPVVRAE LRFK++GV  V+ DV LPWYNAYRF +QN  RL  E
Sbjct:  569 YTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKE 673
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYIC_SCHPO (Isoleucyl-tRNA synthetase, cytoplasmic OS=Schizosaccharomyces pombe GN=SPBC8D2.06 PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 1.759e-31
Identity = 65/99 (65.66%), Postives = 78/99 (78.79%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNA 307
            YTL VL T LF K  Y+N+I +GLV+AEDGKKMSKRLKNYP P  +I  YG+DALRLY+INSPVVRAE L+FK+DGV  VVT V +PW+N+Y+F    A
Sbjct:  566 YTLTVLGTLLFDKAPYKNVIVSGLVMAEDGKKMSKRLKNYPEPNLIIEKYGSDALRLYLINSPVVRAEILKFKEDGVREVVTRVLIPWWNSYKFFEAQA 664
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYI_TETTH (Isoleucyl-tRNA synthetase OS=Tetrahymena thermophila GN=ILSA PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 8.731e-31
Identity = 62/104 (59.62%), Postives = 78/104 (75.00%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEV 322
            YTL V+STAL     Y+NLI NG+VLA DGKKMSK  KNY  P+ + + Y  DA+RLY+INSP+VRAE + FK +GVF V  D+FLPWYNAY+FL+Q+  R E+
Sbjct:  576 YTLNVISTALRNSNPYKNLIVNGIVLAADGKKMSKSKKNYDDPLLIASKYSVDAIRLYMINSPLVRAEEMSFKSEGVFAVKKDIFLPWYNAYKFLIQSITRWEL 679
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYI_BDEBA (Isoleucyl-tRNA synthetase OS=Bdellovibrio bacteriovorus GN=ileS PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 6.662e-30
Identity = 58/109 (53.21%), Postives = 81/109 (74.31%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTL ++ TALF +  ++N++ NGLVLAEDG+KMSK LKNYP PMEV+N +GADALRLY+I+SPVV+A+ L+F + GV+ +V  + L W+N+Y F    A    ++G  P
Sbjct:  572 YTLSIIGTALFNQAPFKNVVVNGLVLAEDGRKMSKSLKNYPDPMEVLNQHGADALRLYLIDSPVVKAQELKFSEKGVYDIVRKILLRWWNSYSFFANYA---NIDGFVP 677

HSP 2 Score: 21.557 bits (44), Expect = 6.662e-30
Identity = 9/22 (40.91%), Postives = 15/22 (68.18%), Query Frame = 3 
Query:  339 FIPLDKVTLEQTSNVLDQWINS 404
            F+P  K   +++ N+LDQW+ S
Sbjct:  675 FVP--KGDAKKSPNILDQWVLS 694
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYIC_ENTBH (Probable isoleucyl-tRNA synthetase, cytoplasmic OS=Enterocytozoon bieneusi (strain H348) GN=EBI_22873 PE=3 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 7.391e-30
Identity = 59/94 (62.77%), Postives = 74/94 (78.72%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRF 292
            YTL V+ST LF  PA++N+I NG+VLAEDGKKMSKRLKNYP PM + N YGADALR+Y+I+SPVV AE LRF ++GV  ++  + +PWYN   F
Sbjct:  603 YTLHVISTILFNSPAFKNVIVNGIVLAEDGKKMSKRLKNYPDPMNIFNKYGADALRMYLISSPVVEAENLRFNENGVKEILKTLLIPWYNTLVF 696
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYIC_YEAST (Isoleucyl-tRNA synthetase, cytoplasmic OS=Saccharomyces cerevisiae GN=ILS1 PE=1 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 1.261e-29
Identity = 60/95 (63.16%), Postives = 77/95 (81.05%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFL 295
            YTL VL T LFG   Y+N+I +G+VLA DG+KMSK LKNYP P  V+N YGADALRLY+INSPV++AE L+FK++GV  VV+ V LPW+N+++FL
Sbjct:  571 YTLAVLGTHLFGSVPYKNVIVSGIVLAADGRKMSKSLKNYPDPSIVLNKYGADALRLYLINSPVLKAESLKFKEEGVKEVVSKVLLPWWNSFKFL 665
[Close]
BLAST of DR067399 vs. DB:swissprot:display
Match: SYIC_ENCCU (Probable isoleucyl-tRNA synthetase, cytoplasmic OS=Encephalitozoon cuniculi GN=ECU11_1100 PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.394e-28
Identity = 55/98 (56.12%), Postives = 77/98 (78.57%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQN 304
            YTL V+S+ LFG+PA+ N++ NG+VLAEDG+KMSKRL+NYP P  + N YGAD+LR+Y+I+SPVV AE L+F + GV  V+  + +PWYN+  F ++N
Sbjct:  578 YTLHVISSLLFGQPAFLNVVVNGIVLAEDGRKMSKRLRNYPDPSHIFNTYGADSLRMYLISSPVVEAENLKFSEGGVKEVLKTLIIPWYNSLGFYLEN 675
[Close]
BLAST of DR067399 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0007s02170.1 ()

HSP 1 Score: 207.223 bits (526), Expect = 6.809e-60
Identity = 101/109 (92.66%), Postives = 105/109 (96.33%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSK LKNYPSPM+VINDYGADALRLY+INSPVVRAE LRFKK+GVF VV DVFLPWYNAYRFLVQNAKRLEVEGLAP
Sbjct:  600 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKSLKNYPSPMDVINDYGADALRLYLINSPVVRAETLRFKKEGVFNVVKDVFLPWYNAYRFLVQNAKRLEVEGLAP 708

HSP 2 Score: 40.4318 bits (93), Expect = 6.809e-60
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + GL  F P+D  TL+ +SNVLDQWINS
Sbjct:  703 VEGLAPFTPIDSATLQDSSNVLDQWINS 730
[Close]
BLAST of DR067399 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0017s06170.1 ()

HSP 1 Score: 208.379 bits (529), Expect = 8.878e-60
Identity = 102/109 (93.58%), Postives = 105/109 (96.33%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSK LKNYPSPMEVINDYGADALRLY+INSPVVRAE LRFKK+GVF VV DVFLPWYNAYRFLVQNAKRLEVEGLAP
Sbjct:  573 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKSLKNYPSPMEVINDYGADALRLYLINSPVVRAETLRFKKEGVFSVVKDVFLPWYNAYRFLVQNAKRLEVEGLAP 681

HSP 2 Score: 38.891 bits (89), Expect = 8.878e-60
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + GL  F P+D  TL+ +SNVLDQWINS
Sbjct:  676 VEGLAPFTPIDAGTLQNSSNVLDQWINS 703
[Close]
BLAST of DR067399 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0017s06170.2 ()

HSP 1 Score: 208.379 bits (529), Expect = 8.878e-60
Identity = 102/109 (93.58%), Postives = 105/109 (96.33%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSK LKNYPSPMEVINDYGADALRLY+INSPVVRAE LRFKK+GVF VV DVFLPWYNAYRFLVQNAKRLEVEGLAP
Sbjct:  573 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKSLKNYPSPMEVINDYGADALRLYLINSPVVRAETLRFKKEGVFSVVKDVFLPWYNAYRFLVQNAKRLEVEGLAP 681

HSP 2 Score: 38.891 bits (89), Expect = 8.878e-60
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + GL  F P+D  TL+ +SNVLDQWINS
Sbjct:  676 VEGLAPFTPIDAGTLQNSSNVLDQWINS 703
[Close]
BLAST of DR067399 vs. P. persica 1.0 peptide
Match: ppa000442m ()

HSP 1 Score: 210.305 bits (534), Expect = 1.094e-59
Identity = 102/109 (93.58%), Postives = 106/109 (97.25%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSK LKNYPSPMEVI+DYGADALRLY+INSPVVRAEPLRFKK+GVFGVV DVFLPWYNAYRFLVQNAKRLEVEG AP
Sbjct:  604 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKSLKNYPSPMEVIDDYGADALRLYLINSPVVRAEPLRFKKEGVFGVVKDVFLPWYNAYRFLVQNAKRLEVEGFAP 712

HSP 2 Score: 36.1946 bits (82), Expect = 1.094e-59
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + G   F P++  T+E++SNVLDQWINS
Sbjct:  707 VEGFAPFRPINHATVEKSSNVLDQWINS 734
[Close]
BLAST of DR067399 vs. P. persica 1.0 peptide
Match: ppa000540m ()

HSP 1 Score: 210.305 bits (534), Expect = 1.094e-59
Identity = 102/109 (93.58%), Postives = 106/109 (97.25%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSK LKNYPSPMEVI+DYGADALRLY+INSPVVRAEPLRFKK+GVFGVV DVFLPWYNAYRFLVQNAKRLEVEG AP
Sbjct:  604 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKSLKNYPSPMEVIDDYGADALRLYLINSPVVRAEPLRFKKEGVFGVVKDVFLPWYNAYRFLVQNAKRLEVEGFAP 712

HSP 2 Score: 36.1946 bits (82), Expect = 1.094e-59
Identity = 15/28 (53.57%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + G   F P++  T+E++SNVLDQWINS
Sbjct:  707 VEGFAPFRPINHATVEKSSNVLDQWINS 734
[Close]
BLAST of DR067399 vs. TrEMBL
Match: B9HH75|B9HH75_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_216568 PE=3 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 1.117e-57
Identity = 101/109 (92.66%), Postives = 105/109 (96.33%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSK LKNYPSPM+VINDYGADALRLY+INSPVVRAE LRFKK+GVF VV DVFLPWYNAYRFLVQNAKRLEVEGLAP
Sbjct:  600 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKSLKNYPSPMDVINDYGADALRLYLINSPVVRAETLRFKKEGVFNVVKDVFLPWYNAYRFLVQNAKRLEVEGLAP 708

HSP 2 Score: 40.4318 bits (93), Expect = 1.117e-57
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + GL  F P+D  TL+ +SNVLDQWINS
Sbjct:  703 VEGLAPFTPIDSATLQDSSNVLDQWINS 730
[Close]
BLAST of DR067399 vs. TrEMBL
Match: B9MY07|B9MY07_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_292610 PE=3 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 1.457e-57
Identity = 102/109 (93.58%), Postives = 105/109 (96.33%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSK LKNYPSPMEVINDYGADALRLY+INSPVVRAE LRFKK+GVF VV DVFLPWYNAYRFLVQNAKRLEVEGLAP
Sbjct:  576 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKSLKNYPSPMEVINDYGADALRLYLINSPVVRAETLRFKKEGVFSVVKDVFLPWYNAYRFLVQNAKRLEVEGLAP 684

HSP 2 Score: 38.891 bits (89), Expect = 1.457e-57
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + GL  F P+D  TL+ +SNVLDQWINS
Sbjct:  679 VEGLAPFTPIDAGTLQNSSNVLDQWINS 706
[Close]
BLAST of DR067399 vs. TrEMBL
Match: A5AU06|A5AU06_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_034773 PE=3 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 1.216e-56
Identity = 98/109 (89.91%), Postives = 104/109 (95.41%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSKRLKNYPSP EVI++YGADALRLYIINSPVVRAEPLRFKK+GV GVV  VFLPWYNAYRFLVQNA+RLEVEG+ P
Sbjct:  593 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPTEVIDEYGADALRLYIINSPVVRAEPLRFKKEGVHGVVKGVFLPWYNAYRFLVQNARRLEVEGVGP 701

HSP 2 Score: 42.3578 bits (98), Expect = 1.216e-56
Identity = 18/28 (64.29%), Postives = 24/28 (85.71%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + G+  FIP+D VTL+++SNVLDQWINS
Sbjct:  696 VEGVGPFIPIDGVTLQKSSNVLDQWINS 723
[Close]
BLAST of DR067399 vs. TrEMBL
Match: D7U1M5|D7U1M5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_102.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00010735001 PE=4 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 1.216e-56
Identity = 98/109 (89.91%), Postives = 104/109 (95.41%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSKRLKNYPSP EVI++YGADALRLYIINSPVVRAEPLRFKK+GV GVV  VFLPWYNAYRFLVQNA+RLEVEG+ P
Sbjct:  556 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPTEVIDEYGADALRLYIINSPVVRAEPLRFKKEGVHGVVKGVFLPWYNAYRFLVQNARRLEVEGVGP 664

HSP 2 Score: 42.3578 bits (98), Expect = 1.216e-56
Identity = 18/28 (64.29%), Postives = 24/28 (85.71%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + G+  FIP+D VTL+++SNVLDQWINS
Sbjct:  659 VEGVGPFIPIDGVTLQKSSNVLDQWINS 686
[Close]
BLAST of DR067399 vs. TrEMBL
Match: B9SUT5|B9SUT5_RICCO (Isoleucyl tRNA synthetase, putative OS=Ricinus communis GN=RCOM_0574620 PE=3 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 6.491e-55
Identity = 97/109 (88.99%), Postives = 106/109 (97.25%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA++NL+CNGLVLAEDGKKMSK+LKNYPSPMEVI+D+GADALRLY+INSPVVRAE LRFKK+GV+ VV DVFLPWYNAYRFLVQNAKRLEVEGLAP
Sbjct:  595 YTLMVLSTALFGKPAFKNLVCNGLVLAEDGKKMSKKLKNYPSPMEVIDDFGADALRLYLINSPVVRAETLRFKKEGVYSVVKDVFLPWYNAYRFLVQNAKRLEVEGLAP 703

HSP 2 Score: 35.039 bits (79), Expect = 6.491e-55
Identity = 16/28 (57.14%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + GL  F PLD   L+ +SNVLD+WINS
Sbjct:  698 VEGLAPFTPLDFGKLQNSSNVLDRWINS 725
[Close]
BLAST of DR067399 vs. TrEMBL
Match: B9FQ68|B9FQ68_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_22145 PE=3 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 2.889e-52
Identity = 93/108 (86.11%), Postives = 102/108 (94.44%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLA 334
            YTLMVLSTALFGKPA++NLICNGLVLAEDGKKMSK  KNYPSP+EVI++YGADALRLY+INSPVVRAE LRFK+ GVFGVV DVFLPWYNAYRFLVQNAKRLE+EG +
Sbjct:  595 YTLMVLSTALFGKPAFKNLICNGLVLAEDGKKMSKSKKNYPSPVEVIDEYGADALRLYLINSPVVRAESLRFKRSGVFGVVKDVFLPWYNAYRFLVQNAKRLEIEGFS 702

HSP 2 Score: 35.039 bits (79), Expect = 2.889e-52
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + G   F P+D+ +L ++SNVLD WINS
Sbjct:  698 IEGFSAFSPIDQASLLKSSNVLDHWINS 725
[Close]
BLAST of DR067399 vs. TrEMBL
Match: B8B0Q4|B8B0Q4_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_23887 PE=3 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 2.889e-52
Identity = 93/108 (86.11%), Postives = 102/108 (94.44%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLA 334
            YTLMVLSTALFGKPA++NLICNGLVLAEDGKKMSK  KNYPSP+EVI++YGADALRLY+INSPVVRAE LRFK+ GVFGVV DVFLPWYNAYRFLVQNAKRLE+EG +
Sbjct:  595 YTLMVLSTALFGKPAFKNLICNGLVLAEDGKKMSKSKKNYPSPVEVIDEYGADALRLYLINSPVVRAESLRFKRSGVFGVVKDVFLPWYNAYRFLVQNAKRLEIEGFS 702

HSP 2 Score: 35.039 bits (79), Expect = 2.889e-52
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + G   F P+D+ +L ++SNVLD WINS
Sbjct:  698 IEGFSAFSPIDQASLLKSSNVLDHWINS 725
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BLAST of DR067399 vs. TrEMBL
Match: Q67WM2|Q67WM2_ORYSJ (Putative isoleucine-tRNA ligase OS=Oryza sativa subsp. japonica GN=P0017B12.25-1 PE=3 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 2.896e-52
Identity = 93/108 (86.11%), Postives = 102/108 (94.44%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLA 334
            YTLMVLSTALFGKPA++NLICNGLVLAEDGKKMSK  KNYPSP+EVI++YGADALRLY+INSPVVRAE LRFK+ GVFGVV DVFLPWYNAYRFLVQNAKRLE+EG +
Sbjct:  595 YTLMVLSTALFGKPAFKNLICNGLVLAEDGKKMSKSKKNYPSPVEVIDEYGADALRLYLINSPVVRAESLRFKRSGVFGVVKDVFLPWYNAYRFLVQNAKRLEIEGFS 702

HSP 2 Score: 35.039 bits (79), Expect = 2.896e-52
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + G   F P+D+ +L ++SNVLD WINS
Sbjct:  698 IEGFSAFSPIDQASLLKSSNVLDHWINS 725
[Close]
BLAST of DR067399 vs. TrEMBL
Match: A9TB11|A9TB11_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_220535 PE=3 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.847e-51
Identity = 88/111 (79.28%), Postives = 100/111 (90.09%), Query Frame = 2 
Query:    5 RVYTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            R YTLMVLSTALF +PA++NLICNGLVLAEDGKKMSKRL NYP P ++IN+YGADALRLY+INSPVV+ EPLRFK++GV+ VV DVFLPWYNAYRFLVQN KRL+ EGL P
Sbjct:  592 RFYTLMVLSTALFDRPAFQNLICNGLVLAEDGKKMSKRLNNYPPPTDIINEYGADALRLYLINSPVVKGEPLRFKREGVYSVVKDVFLPWYNAYRFLVQNVKRLDAEGLEP 702

HSP 2 Score: 37.3502 bits (85), Expect = 1.847e-51
Identity = 16/26 (61.54%), Postives = 19/26 (73.08%), Query Frame = 3 
Query:  327 GLHRFIPLDKVTLEQTSNVLDQWINS 404
            GL  F PLD+  L Q+ NVLD+WINS
Sbjct:  699 GLEPFKPLDRAVLRQSPNVLDRWINS 724
[Close]
BLAST of DR067399 vs. TrEMBL
Match: B8AIX8|B8AIX8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_06123 PE=3 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 1.178e-50
Identity = 95/108 (87.96%), Postives = 101/108 (93.52%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLA 334
            YTLMVLSTALFGKPA++NLICNGLVLAEDGKKMSK   NYPSPMEVI++YGADALRLY+INSPVVRAE LRF K+GV GVV DVFLPWYNAYRFLVQNAKRLEVEGLA
Sbjct: 1149 YTLMVLSTALFGKPAFKNLICNGLVLAEDGKKMSKSKNNYPSPMEVIDNYGADALRLYVINSPVVRAESLRFNKNGVHGVVKDVFLPWYNAYRFLVQNAKRLEVEGLA 1256

HSP 2 Score: 30.0314 bits (66), Expect = 1.178e-50
Identity = 13/28 (46.43%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + GL  F P+++  L ++SNVLD WI S
Sbjct: 1252 VEGLAVFSPINQAILLRSSNVLDHWIQS 1279
[Close]
BLAST of DR067399 vs. Vitis vinifera peptide
Match: GSVIVT01010735001 (assembled CDS)

HSP 1 Score: 201.83 bits (512), Expect = 4.660e-59
Identity = 98/109 (89.91%), Postives = 104/109 (95.41%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALFGKPA+RNLICNGLVLAEDGKKMSKRLKNYPSP EVI++YGADALRLYIINSPVVRAEPLRFKK+GV GVV  VFLPWYNAYRFLVQNA+RLEVEG+ P
Sbjct:  556 YTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPTEVIDEYGADALRLYIINSPVVRAEPLRFKKEGVHGVVKGVFLPWYNAYRFLVQNARRLEVEGVGP 664

HSP 2 Score: 42.3578 bits (98), Expect = 4.660e-59
Identity = 18/28 (64.29%), Postives = 24/28 (85.71%), Query Frame = 3 
Query:  321 LRGLHRFIPLDKVTLEQTSNVLDQWINS 404
            + G+  FIP+D VTL+++SNVLDQWINS
Sbjct:  659 VEGVGPFIPIDGVTLQKSSNVLDQWINS 686
[Close]
BLAST of DR067399 vs. TAIR peptide
Match: AT4G10320.1 (| Symbols: | tRNA synthetase class I (I, L, M and V) family protein | chr4:6397526-6404509 REVERSE LENGTH=1190)

HSP 1 Score: 192.2 bits (487), Expect = 3.143e-52
Identity = 91/109 (83.49%), Postives = 102/109 (93.58%), Query Frame = 2 
Query:   11 YTLMVLSTALFGKPAYRNLICNGLVLAEDGKKMSKRLKNYPSPMEVINDYGADALRLYIINSPVVRAEPLRFKKDGVFGVVTDVFLPWYNAYRFLVQNAKRLEVEGLAP 337
            YTLMVLSTALF KPA++NLICNGLVLAEDGKKM+K+L+NYP P+EVI++YGADA+RLY+INSPVVRAEPLRFKK+GV GVV DVFLPWYNAYRFLVQNAKRLE EG  P
Sbjct:  601 YTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAVRLYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVP 709

HSP 2 Score: 29.6462 bits (65), Expect = 3.143e-52
Identity = 12/22 (54.55%), Postives = 18/22 (81.82%), Query Frame = 3 
Query:  339 FIPLDKVTLEQTSNVLDQWINS 404
            F+P D  T+ Q++N+LDQWI+S
Sbjct:  710 FVPTDLATI-QSANILDQWIHS 730
The following BLAST results are available for this feature:
BLAST of DR067399 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
SYIC_HUMAN2.534e-3870.48Isoleucyl-tRNA synthetase, cytoplasmic OS=Homo sap... [more]
SYIC_DICDI9.631e-3871.84Probable isoleucyl-tRNA synthetase, cytoplasmic OS... [more]
SYIC_CAEEL9.631e-3869.52Isoleucyl-tRNA synthetase, cytoplasmic OS=Caenorha... [more]
SYIC_MOUSE1.643e-3768.57Isoleucyl-tRNA synthetase, cytoplasmic OS=Mus musc... [more]
SYIC_SCHPO1.759e-3165.66Isoleucyl-tRNA synthetase, cytoplasmic OS=Schizosa... [more]
SYI_TETTH8.731e-3159.62Isoleucyl-tRNA synthetase OS=Tetrahymena thermophi... [more]
SYI_BDEBA6.662e-3053.21Isoleucyl-tRNA synthetase OS=Bdellovibrio bacterio... [more]
SYIC_ENTBH7.391e-3062.77Probable isoleucyl-tRNA synthetase, cytoplasmic OS... [more]
SYIC_YEAST1.261e-2963.16Isoleucyl-tRNA synthetase, cytoplasmic OS=Saccharo... [more]
SYIC_ENCCU1.394e-2856.12Probable isoleucyl-tRNA synthetase, cytoplasmic OS... [more]
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BLAST of DR067399 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
POPTR_0007s02170.16.809e-6092.66[more]
POPTR_0017s06170.18.878e-6093.58[more]
POPTR_0017s06170.28.878e-6093.58[more]
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BLAST of DR067399 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
ppa000442m1.094e-5993.58[more]
ppa000540m1.094e-5993.58[more]
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BLAST of DR067399 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10
Match NameE-valueIdentityDescription
B9HH75|B9HH75_POPTR1.117e-5792.66Predicted protein (Fragment) OS=Populus trichocarp... [more]
B9MY07|B9MY07_POPTR1.457e-5793.58Predicted protein (Fragment) OS=Populus trichocarp... [more]
A5AU06|A5AU06_VITVI1.216e-5689.91Putative uncharacterized protein OS=Vitis vinifera... [more]
D7U1M5|D7U1M5_VITVI1.216e-5689.91Whole genome shotgun sequence of line PN40024, sca... [more]
B9SUT5|B9SUT5_RICCO6.491e-5588.99Isoleucyl tRNA synthetase, putative OS=Ricinus com... [more]
B9FQ68|B9FQ68_ORYSJ2.889e-5286.11Putative uncharacterized protein OS=Oryza sativa s... [more]
B8B0Q4|B8B0Q4_ORYSI2.889e-5286.11Putative uncharacterized protein OS=Oryza sativa s... [more]
Q67WM2|Q67WM2_ORYSJ2.896e-5286.11Putative isoleucine-tRNA ligase OS=Oryza sativa su... [more]
A9TB11|A9TB11_PHYPA1.847e-5179.28Predicted protein OS=Physcomitrella patens subsp. ... [more]
B8AIX8|B8AIX8_ORYSI1.178e-5087.96Putative uncharacterized protein OS=Oryza sativa s... [more]
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BLAST of DR067399 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1
Match NameE-valueIdentityDescription
GSVIVT010107350014.660e-5989.91assembled CDS[more]
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BLAST of DR067399 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT4G10320.13.143e-5283.49| Symbols: | tRNA synthetase class I (I, L, M and... [more]
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InterPro
Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 3..71
score: 2.8
IPR002301Isoleucyl-tRNA synthetase, class IaPANTHERPTHR11946:SF9ISOLEUCYL TRNA SYNTHETASEcoord: 4..108
score: 4.5
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620no descriptioncoord: 4..104
score: 4
NoneNo IPR availablePANTHERPTHR11946ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASEScoord: 4..108
score: 4.5
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 3..86
score: 1.9

Properties
Property NameValue
Singlet in analysisVaccinium corymbosum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>DR067399 ID=DR067399; Name=DR067399; organism=Vaccinium corymbosum; type=EST; length=405bp
CACGAGGGTCTACACTCTCATGGTCTTGTCAACTGCACTTTTTGGAAAGC
CTGCGTATAGGAACCTCATTTGCAATGGACTTGTCCTGGCTGAGGATGGT
AAAAAAATGAGTAAAAGACTCAAGAACTATCCATCACCTATGGAAGTCAT
AAATGATTATGGGGCTGATGCTTTGCGATTATACATTATTAACTCTCCAG
TGGTGCGAGCTGAGCCTTTACGTTTTAAAAAGGATGGAGTATTTGGTGTT
GTGACAGATGTCTTTTTACCATGGTACAATGCATATCGATTCCTTGTTCA
GAATGCAAAGAGACTAGAGGTTGAGGGGCTTGCACCGGTTCATTCCTCTT
GATAAAGTCACACTTGAGCAGACATCTAATGTTCTTGACCAGTGGATCAA
CTCAG
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
GO:0004822isoleucine-tRNA ligase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Biological Process
TermDefinition
GO:0006412translation
GO:0006418tRNA aminoacylation for protein translation
GO:0006428isoleucyl-tRNA aminoacylation
Vocabulary: KEGG ORTHOLOGY
TermDefinition
K01870IARS, ileS; isoleucyl-tRNA synthetase [EC:6.1.1.5]
Vocabulary: KEGG PATHWAY
TermDefinition
ko00250Alanine, aspartate and glutamate metabolism
ko03010Ribosome
Vocabulary: INTERPRO
TermDefinition
IPR002300aa-tRNA-synth_Ia
IPR002301Ile-tRNA-ligase
IPR014729Rossmann-like_a/b/a_fold
noIPRunintegrated