DW043525 | GDV

EST Overview


Name	DW043525
Unique Name	DW043525
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	792

Libraries


Library Name	Type
LIBEST_018810 Subtracted cDNA library of Vaccinium corymbosum	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of DW043525 vs. DB:swissprot:display
Match: PSAB_PINTH (Photosystem I P700 chlorophyll a apoprotein A2 OS=Pinus thunbergii GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 4.894e-8
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. DB:swissprot:display
Match: PSAB_PINKO (Photosystem I P700 chlorophyll a apoprotein A2 OS=Pinus koraiensis GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 4.894e-8
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. DB:swissprot:display
Match: PSAB_MESVI (Photosystem I P700 chlorophyll a apoprotein A2 OS=Mesostigma viride GN=psaB PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 6.391e-8
Identity = 43/116 (37.07%), Postives = 54/116 (46.55%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IYH***WDG*EI--------ILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KH+TLWQGNV           +D             LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHLTLWQGNVAQ---------FDESSTYLMGWLRDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLIWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. DB:swissprot:display
Match: PSAB_ANGEV (Photosystem I P700 chlorophyll a apoprotein A2 OS=Angiopteris evecta GN=psaB PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.860e-7
Identity = 43/116 (37.07%), Postives = 53/116 (45.69%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IYH***WDG*EI--------ILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV           +D             LWLNS+QLINGYNP        FG     +W          WA G+M+  ISW
Sbjct:  571 SAWDAFYLSVFWMLNTIGWVTFYW-HWKHITLWQGNVAQ---------FDESSTYLMGWLRDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWAIGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. DB:swissprot:display
Match: PSAB_EUGGR (Photosystem I P700 chlorophyll a apoprotein A2 OS=Euglena gracilis GN=psaB PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 2.429e-7
Identity = 44/110 (40.00%), Postives = 53/110 (48.18%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNV--IAV**IYH***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LW------TSR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        Y           LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWTTFYW-HWKHITLWQGNVGQFNESSTYL---MGWLRDYLWLNSSQLINGYNP--------FGMNSLAVWGWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: B8Y2X6|B8Y2X6_FESAR (Photosystem I P700 chlorophyll a apoprotein OS=Festuca arundinacea GN=psaB PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.776e-9
Identity = 46/112 (41.07%), Postives = 55/112 (49.11%), Query Frame = 1

Query:   43 DAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IYH***WDG*EII-------LWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            DAFY+   LDVKYH M   ++ H KHITLWQGNV            +    +       LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  577 DAFYLAVLLDVKYHWMRLLFHWHWKHITLWQGNVSQF--------NESSTYLMGWLRDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 671

[Close]

BLAST of DW043525 vs. TrEMBL
Match: Q4FFK1|Q4FFK1_GINBI (Photosystem I P700 chlorophyll a apoprotein (Fragment) OS=Ginkgo biloba GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: C3W2M3|C3W2M3_PINPS (Photosystem I P700 chlorophyll a apoprotein OS=Pinus pinaster GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: C3W2F8|C3W2F8_PINPE (Photosystem I P700 chlorophyll a apoprotein OS=Pinus peuce GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: C3W294|C3W294_PINPR (Photosystem I P700 chlorophyll a apoprotein OS=Pinus parviflora var. pentaphylla GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: C3W234|C3W234_PINMO (Photosystem I P700 chlorophyll a apoprotein OS=Pinus monticola GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: C3W1X1|C3W1X1_9CONI (Photosystem I P700 chlorophyll a apoprotein OS=Pinus merkusii GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: C3W1J3|C3W1J3_PINLA (Photosystem I P700 chlorophyll a apoprotein OS=Pinus lambertiana GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: C3W1C9|C3W1C9_9CONI (Photosystem I P700 chlorophyll a apoprotein OS=Pinus flexilis GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TrEMBL
Match: C3W164|C3W164_PINLE (Photosystem I P700 chlorophyll a apoprotein OS=Pinus leiophylla var. chihuahuana GN=psaB PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 7.560e-7
Identity = 44/109 (40.37%), Postives = 54/109 (49.54%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IY-H***WDG*EIILWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV        +   W      LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVAQFNESSTYLMGWS--RDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

[Close]

BLAST of DW043525 vs. TAIR peptide
Match: ATCG00340.1 (| Symbols: PSAB | Photosystem I, PsaA/PsaB protein | chrC:37375-39579 REVERSE LENGTH=734)

HSP 1 Score: 56.9954 bits (136), Expect = 1.414e-8
Identity = 43/115 (37.39%), Postives = 54/115 (46.96%), Query Frame = 1

Query:   34 TAWDAFYIGGFLDVKYHRMGDYYYRHRKHITLWQGNVIAV**IYH***WDG*EII-------LWLNSTQLINGYNPIRYE*YYRFGRGCY*LWT------SR*WATGYMYTKISW 339
            +AWDAFY+  F  +       +Y+ H KHITLWQGNV            +    +       LWLNS+QLINGYNP        FG     +W          WATG+M+  ISW
Sbjct:  571 SAWDAFYLAVFWMLNTIGWVTFYW-HWKHITLWQGNVSQF--------NESSTYLMGWLRDYLWLNSSQLINGYNP--------FGMNSLSVWAWMFLFGHLVWATGFMFL-ISW 667

The following BLAST results are available for this feature:

BLAST of DW043525 vs. DB:swissprot:display
BLAST of DW043525 vs. TrEMBL
BLAST of DW043525 vs. TAIR peptide

BLAST of DW043525 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 5


Match Name	E-value	Identity	Description
PSAB_PINTH	4.894e-8	40.37	Photosystem I P700 chlorophyll a apoprotein A2 OS=...	[more]
PSAB_PINKO	4.894e-8	40.37	Photosystem I P700 chlorophyll a apoprotein A2 OS=...	[more]
PSAB_MESVI	6.391e-8	37.07	Photosystem I P700 chlorophyll a apoprotein A2 OS=...	[more]
PSAB_ANGEV	1.860e-7	37.07	Photosystem I P700 chlorophyll a apoprotein A2 OS=...	[more]
PSAB_EUGGR	2.429e-7	40.00	Photosystem I P700 chlorophyll a apoprotein A2 OS=...	[more]

BLAST of DW043525 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
B8Y2X6\|B8Y2X6_FESAR	2.776e-9	41.07	Photosystem I P700 chlorophyll a apoprotein OS=Fes...	[more]
Q4FFK1\|Q4FFK1_GINBI	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein (Fragm...	[more]
C3W2M3\|C3W2M3_PINPS	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein OS=Pin...	[more]
C3W2F8\|C3W2F8_PINPE	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein OS=Pin...	[more]
C3W294\|C3W294_PINPR	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein OS=Pin...	[more]
C3W234\|C3W234_PINMO	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein OS=Pin...	[more]
C3W1X1\|C3W1X1_9CONI	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein OS=Pin...	[more]
C3W1J3\|C3W1J3_PINLA	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein OS=Pin...	[more]
C3W1C9\|C3W1C9_9CONI	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein OS=Pin...	[more]
C3W164\|C3W164_PINLE	7.560e-7	40.37	Photosystem I P700 chlorophyll a apoprotein OS=Pin...	[more]

BLAST of DW043525 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ATCG00340.1	1.414e-8	37.39	\| Symbols: PSAB \| Photosystem I, PsaA/PsaB protein...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 1..12 score: NAcoord: 13..28 score
None	No IPR available	SEG	seg	seg	coord: 70..80 score
None	No IPR available	SEG	seg	seg	coord: 10..31 score
None	No IPR available	SEG	seg	seg	coord: 4..14 score
None	No IPR available	SEG	seg	seg	coord: 30..48 score
None	No IPR available	SEG	seg	seg	coord: 18..31 score
IPR001280	Photosystem I PsaA/PsaB	PFAM	PF00223	PsaA_PsaB	coord: 7..53 score: 0.0

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DW043525 ID=DW043525; Name=DW043525; organism=Vaccinium corymbosum; type=EST; length=792bp
AGCGTGGTCGCGGCCGAGGTACTTGTGATATTTACGGCTTGGGATGCATT
TTATATTGGCGGTTTTCTGGATGTTAAATACCATAGGATGGGTGACTATT
ATTATAGGCATAGGAAGCACATCACGTTATGGCAGGGTAACGTTATCGCA
GTTTAATGAATCTACCACTAATAATGATGGGATGGTTAAGAGATTATATT
ATGGTTAAACTCTACCCAACTAATCAATGGATATAACCCTATTAGGTATG
AATAGTATTATCGGTTTGGGCGTGGATGTTACTAATTATGGACATCTAGA
TAATGGGCTACTGGATATATGTATACTAAAATTTCCTGGCCGAGGAATAT
AGGGCAGGGAAATAGAATAGGAAAACTTATAGACATTGGGGCCCTCAACC
GAAACGTTTACAAACCCTACCCCCAGGTTATTATATCTACCCCCATCACT
TAGGAAATTAAAAATTTTCCCCCTAAAATTTTTTTCACATTTANCCANGA
AAGTTTGGGAAACCCCTAACCCANCAAAAGGGGGGCCCCCCCACCCCATT
TTTTCCGCCAAAAAACCTTCCGGGNTTGAACCCGGGGCCCCAAATTTGCC
CAAAAATTTAAAAAACCCCCCCACCCCCATTTAAGGCCCCAAAAATTTCT
TTTCCCCCCAATTTTTCCCCCCCCCCTTTTTCTTTAACAAGTCCCATTTT
CCCCGGCCCGCCCCCTAAAAAAAGANNANCCTCCTTTTTTGAAACCTTTT
GGAAACCCCCCCCCCTTCNCTTTCCCACCTGGAATTTGGAGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0009522	photosystem I
GO:0009579	thylakoid
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0015979	photosynthesis

Vocabulary: **INTERPRO**
Term	Definition
IPR001280	PSI_PsaA/B
noIPR	unintegrated