CF810537 | GDV

EST Overview


Name	CF810537
Unique Name	CF810537
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	648

Libraries


Library Name	Type
LIBEST_014530 cDNA cold acclimated Bluecrop library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: QTRD1_XENTR (Queuine tRNA-ribosyltransferase subunit qtrtd1 OS=Xenopus tropicalis GN=qtrtd1 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 3.303e-19
Identity = 70/209 (33.49%), Postives = 102/209 (48.80%), Query Frame = 2

Query:   29 GRILIKPSEYMKFIDSMKPNSWTTLADE--VPAWVSQKRNKTSVDRTVRWLDDCIALSS-----KGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTF 634
            GRI +   +++     ++P+ +  L+D    P   S+KR K SVDRT+ +LD+C+ L S     K   + GA+ GG  +EER RSA+E AKR                                          P+  + + GF  G S +    L++AVT +LPE KPR I G+G P+EVL+ +  GVDLFD+ + Y +T  G AL F
Sbjct:  120 GRIEMTAQKFISAQRVLRPDWFQCLSDGEVTPGGNSRKRVKKSVDRTLAFLDECLQLLSGHEDLKPCVLIGAVEGGDLLEERLRSARETAKR------------------------------------------PVGGFLLDGFH-GGSAEKELSLISAVTAALPEDKPRFIHGMGRPDEVLECVQRGVDLFDSCFPYRVTERGCALIF 285

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: QTRD1_MOUSE (Queuine tRNA-ribosyltransferase subunit QTRTD1 OS=Mus musculus GN=Qtrtd1 PE=1 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 9.609e-19
Identity = 68/214 (31.78%), Postives = 102/214 (47.66%), Query Frame = 2

Query:   29 GRILIKPSEYMKFIDSMKPNSWTTLAD---EVPAWVSQKRNKTSVDRTVRWLDDCIALSSKG-----GNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGF-GLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            GR+ +  S++M   ++++P+ +  L+D         S KR + SVDR++ +LD C+ L  +        I G I GG  +EER RSA+E AKR                                          P+  + + GF G     + R  LL++VT  LPE KPR ICG+  P+EVL+ I+ GVDLF++ + Y +T  G ALTF  +
Sbjct:  120 GRVEMTVSKFMAIQEALQPDWFQCLSDGEASCAETTSIKRARKSVDRSLLFLDSCLRLQEESEVLQKSVIIGVIEGGDVMEERLRSARETAKR------------------------------------------PVGGFLLDGFQGDPAVTETRLHLLSSVTAELPEDKPRLICGVSRPDEVLECIERGVDLFESFFPYQVTERGCALTFTFD 291

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: QTRD1_CHICK (Queuine tRNA-ribosyltransferase subunit QTRTD1 OS=Gallus gallus GN=QTRTD1 PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 9.609e-19
Identity = 66/210 (31.43%), Postives = 105/210 (50.00%), Query Frame = 2

Query:   29 GRILIKPSEYMKFIDSMKPNSWTTLAD--EVPAWVSQKRNKTSVDRTVRWLDDCIALSSK-----GGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGF-GLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTF 634
            GR+ +  S++M    +++P+ +  +AD   +   V++KR K SVDR++ +LD C+ L  K     G  +FG I GG  +EER RSA+E AKR                                          P+  + + GF G   + + +  L+++VT  LPE KPR I G+G P+EVL+ I+ GVD+F++ + + +T  G AL F
Sbjct:  119 GRMEMTASKFMDIQRAIQPDWFQCIADGDTISGEVTRKRAKKSVDRSLSFLDACLQLLEKTPELQGSVMFGTIEGGDVLEERLRSARETAKR------------------------------------------PVGGFLLDGFQGRAMAKETKMNLISSVTAELPEDKPRIIHGVGKPDEVLECIERGVDIFESFFPFQVTERGCALVF 286

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: QTRD1_XENLA (Queuine tRNA-ribosyltransferase subunit qtrtd1 OS=Xenopus laevis GN=qtrtd1 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 4.769e-18
Identity = 67/210 (31.90%), Postives = 102/210 (48.57%), Query Frame = 2

Query:   29 GRILIKPSEYMKFIDSMKPNSWTTLADE--VPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGNI-----FGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGF-GLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTF 634
            GRI +   +++     ++P+ +  L+D    P   S+KR K SVDR++ +LD+C+ L S+   +      GA+ GG  ++ER RSA+E AKR                                          P+  + + GF G+    + +  L++AVT  LPE KPR I G+G P+EVL+ I  GVDLFD+ + Y +T  G AL F
Sbjct:  120 GRIEMTTQKFISAQRVLRPDWFQCLSDGEVTPGGNSRKRIKKSVDRSLVFLDECLQLLSEHEELKPCVLIGAVEGGDLLDERLRSARETAKR------------------------------------------PVGGFLLDGFHGISAGNEAKLSLVSAVTAELPEDKPRFIHGVGRPDEVLEFIQRGVDLFDSCFPYQVTERGCALIF 287

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: QTRD1_PONAB (Queuine tRNA-ribosyltransferase subunit QTRTD1 OS=Pongo abelii GN=QTRTD1 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 4.769e-18
Identity = 67/214 (31.31%), Postives = 103/214 (48.13%), Query Frame = 2

Query:   29 GRILIKPSEYMKFIDSMKPNSWTTLAD---EVPAWVSQKRNKTSVDRTVRWLDDCIALSSKG-----GNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGF-GLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            GR+ +  S++M    +++P+ +  L+D         S KR + SVDR++ +LD+C+ L  +        I G I GG  +EER RSA+E AKR                                          P+  + + GF G   +++ R  LL++VT  LPE KPR I G+  P+EVL+ I+ GVDLF++ + Y +T  G ALTF  +
Sbjct:  120 GRVEMTVSKFMAIQQALQPDWFQCLSDGEVSCKEATSIKRVRKSVDRSLLFLDNCLRLQEESEVLQKSVIIGVIEGGDVMEERLRSARETAKR------------------------------------------PVGGFLLDGFQGNPTTLETRLRLLSSVTAELPEDKPRLISGVSRPDEVLECIERGVDLFESFFPYQVTERGCALTFSFD 291

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: QTRD1_HUMAN (Queuine tRNA-ribosyltransferase subunit QTRTD1 OS=Homo sapiens GN=QTRTD1 PE=1 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 8.134e-18
Identity = 67/214 (31.31%), Postives = 103/214 (48.13%), Query Frame = 2

Query:   29 GRILIKPSEYMKFIDSMKPNSWTTLAD---EVPAWVSQKRNKTSVDRTVRWLDDCIALSSKG-----GNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGF-GLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            GR+ +  S++M    +++P+ +  L+D         S KR + SVDR++ +LD+C+ L  +        I G I GG  +EER RSA+E AKR                                          P+  + + GF G   +++ R  LL++VT  LPE KPR I G+  P+EVL+ I+ GVDLF++ + Y +T  G ALTF  +
Sbjct:  120 GRVEMTVSKFMAIQKALQPDWFQCLSDGEVSCKEATSIKRVRKSVDRSLLFLDNCLRLQEESEVLQKSVIIGVIEGGDVMEERLRSARETAKR------------------------------------------PVGGFLLDGFQGNPTTLEARLRLLSSVTAELPEDKPRLISGVSRPDEVLECIERGVDLFESFFPYQVTERGCALTFSFD 291

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: QTRD1_DANRE (Queuine tRNA-ribosyltransferase subunit qtrtd1 OS=Danio rerio GN=qtrtd1 PE=2 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 1.812e-17
Identity = 61/210 (29.05%), Postives = 100/210 (47.62%), Query Frame = 2

Query:   29 GRILIKPSEYMKFIDSMKPNSWTTLADEVP--AWVSQKRNKTSVDRTVRWLDDCIALSSKG-----GNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGF-GLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTF 634
            GRI +  + +M    +++P+ + ++AD     A  S+KR + +VDRT+  LD+C+ L  K        IFG + GG  +EER RSA+E AKR                                          P+  + + GF       D R  L+   +  LP++KPR + G+G P+EV+  +++GVDLF++ + + +T  G AL+F
Sbjct:  120 GRIELTAARFMSIQAAVQPDCYQSMADGETWQANTSRKRVRKAVDRTLAHLDECLVLHQKTQELKHAEIFGVVEGGDILEERLRSARETAKR------------------------------------------PVGGFVLDGFHSSAMDQDVRAQLIQETSAELPQEKPRLVLGVGRPDEVISCVEAGVDLFESFFPFQVTERGCALSF 287

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: QTRD1_TRIAD (Queuine tRNA-ribosyltransferase subunit QTRTD1 homolog OS=Trichoplax adhaerens GN=TRIADDRAFT_53275 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.708e-13
Identity = 53/180 (29.44%), Postives = 82/180 (45.56%), Query Frame = 2

Query:   20 TPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIAL-----SSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGE-SMDDRPPLLNAVTDSLPEQKPRQICGLGLP 541
            T  GR  + P +Y KF++  +P+ + +L D VPA  S KR + SVDRT+ +LD C+       S K   ++ A+ GG  V+ER+RSA+E+A R                                          P+  + + GFG  + +++    LL   T +LP +KPR I  +G P
Sbjct:  118 TKTGRTKVTPDDYSKFVEVCRPSWYQSLCDTVPANASIKRTRKSVDRTLEFLDQCLKYREKHDSLKTSELWAAVEGGGLVDERQRSAKESATR------------------------------------------PVFGFTLEGFGSDQMNVETIFELLPLTTQNLPVEKPRLIHAIGSP 255

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: TGT_DEIRA (Queuine tRNA-ribosyltransferase OS=Deinococcus radiodurans GN=tgt PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.292e-12
Identity = 50/176 (28.41%), Postives = 76/176 (43.18%), Query Frame = 2

Query:  107 DEVPAWVSQKRN-KTSVDRTVRWLDDCIALSSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALT 631
            DE P + +++   + S+DRTVRWL+ C A+ +K      AIV G   E+ R  + EA                    PF                           + +GG  +GES ++  P +      LPE KPR + G+G PE+++ G+  G+D+FD  Y       G+ALT
Sbjct:  153 DECPPYPAERPYIEASLDRTVRWLERCHAVKTKDDQALFAIVQGGVHEDLRLKSLEAT------------------LPFA-----------------------TPGFAVGGLAVGESKEEMYPAVAFTAGRLPENKPRYLMGVGHPEDLVAGVALGIDMFDCVYPTRTGRFGYALT 287

[Close]

BLAST of CF810537 vs. DB:swissprot:display
Match: TGT_XENTR (Queuine tRNA-ribosyltransferase OS=Xenopus tropicalis GN=qtrt1 PE=2 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 2.535e-11
Identity = 54/212 (25.47%), Postives = 87/212 (41.04%), Query Frame = 2

Query:    5 GASFETPFG--RILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGG--NIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFAL 628
            G  F +P+    IL+ P + ++  +++  +    L D V + ++  R + ++ R++RWLD CIA +S     N+F  I GG   E RR+  QE  KR+V                                            + IGG   GE  D    ++   TD LP  KPR + G+G   +++  +  G D+FD  +       G AL
Sbjct:  118 GVQFRSPYDGKEILLTPEKSIEIQNALGSDIMMQLDDVVSSTITGPRVEEAMHRSIRWLDRCIAANSNPDRQNLFAIIQGGLDAELRRKCLQEMTKRDV------------------------------------------PGFAIGGLSGGEEKDHFWRMVTLSTDHLPRDKPRYLMGVGYATDLVVCVALGCDMFDCVFPTRTARFGSAL 287

[Close]

BLAST of CF810537 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0019s10480.1 ()

HSP 1 Score: 248.054 bits (632), Expect = 3.545e-66
Identity = 128/214 (59.81%), Postives = 145/214 (67.76%), Query Frame = 2

Query:    2 LGASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            LGASFETP GR+LIKP+EYM  I S++PN W TLADEVPAWVS+KRNK SVDRTV+WLD+C+ALS   G  FGAIVGGS VEERRR AQE  KRNVS                                           YWIGGFG GES D+RP LL+AVTD LP+  PR I GLGLPEEVLQG+ +G+DLFD++YIYHLT GGFALTFPL+
Sbjct:  116 LGASFETPCGRLLIKPAEYMGIISSLRPNLWATLADEVPAWVSKKRNKNSVDRTVKWLDECVALSPASGVAFGAIVGGSDVEERRRCAQEVVKRNVS------------------------------------------GYWIGGFGFGESTDERPALLSAVTDILPKDGPRLISGLGLPEEVLQGVAAGIDLFDSSYIYHLTIGGFALTFPLD 287

[Close]

BLAST of CF810537 vs. P. persica 1.0 peptide
Match: ppa023702m ()

HSP 1 Score: 248.44 bits (633), Expect = 1.919e-66
Identity = 125/213 (58.69%), Postives = 148/213 (69.48%), Query Frame = 2

Query:    5 GASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            GASFETP GR LIKP EY++ I S++PN WTTLADEVP WVS+KRNKTSVDRT+RWLD+CIAL+  GG +FG+IVGG  V+ER R AQEAA+RN+S                                           YWIGGFGLGESMD+RP LLNAVTD L E+KPR I GLGLPEE+LQG+ +G+D+FD+TY+ HLT GGFALTFPL+
Sbjct:  114 GASFETPCGRRLIKPVEYVELISSLRPNIWTTLADEVPTWVSEKRNKTSVDRTIRWLDECIALNPAGGPVFGSIVGGCSVKERERCAQEAARRNLS------------------------------------------GYWIGGFGLGESMDERPALLNAVTDILQEEKPRLITGLGLPEEILQGVAAGIDVFDSTYLCHLTLGGFALTFPLD 284

[Close]

BLAST of CF810537 vs. TrEMBL
Match: D7TLI8|D7TLI8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_82.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036437001 PE=4 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 2.766e-66
Identity = 132/214 (61.68%), Postives = 147/214 (68.69%), Query Frame = 2

Query:    2 LGASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            +GASFETP GR LIKP EYM+ I SMKP+ W TLADEVPAWVS KRNK SVDRT+RWLD+CI LS  GG +FGAIVGG+ VEER+R AQE A RNVS                                           YWIGGFGLGESMD+RP LLNAV D LP ++PRQICGL LPEEVLQG+ +G+DLFD+TYIYHLT GGFALTFPLE
Sbjct:  113 IGASFETPSGRRLIKPVEYMEMISSMKPSLWATLADEVPAWVSAKRNKASVDRTLRWLDECITLSPAGGAVFGAIVGGANVEERQRCAQEVAGRNVS------------------------------------------GYWIGGFGLGESMDERPALLNAVADILPVERPRQICGLELPEEVLQGVAAGIDLFDSTYIYHLTLGGFALTFPLE 284

[Close]

BLAST of CF810537 vs. TrEMBL
Match: C6T7F2|C6T7F2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 2.192e-63
Identity = 122/215 (56.74%), Postives = 149/215 (69.30%), Query Frame = 2

Query:    2 LGASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLEK 646
            LGASFETP GR+LIKP +Y++ I  M+PN W TLADEVPAWV+ KRNKTSVDRTVRWLDDC+AL+   G++FGAIVGG+ ++ER+R A+E AKRNVS                                           YWIGGFGLGE +D+RP LL+A+ D LP++KPR I GLGLPEE+L+GID+G+DLFD+TYIY LT GGFALTF L+K
Sbjct:  118 LGASFETPCGRLLIKPKDYVEMISCMRPNIWATLADEVPAWVTDKRNKTSVDRTVRWLDDCLALNPVVGSVFGAIVGGTNLDERKRCAEEVAKRNVS------------------------------------------GYWIGGFGLGEGIDERPALLSAIIDLLPDEKPRMISGLGLPEEILEGIDAGIDLFDSTYIYSLTLGGFALTFSLDK 290

[Close]

BLAST of CF810537 vs. TrEMBL
Match: B9IQC4|B9IQC4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_574205 PE=4 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 3.747e-55
Identity = 114/198 (57.58%), Postives = 130/198 (65.66%), Query Frame = 2

Query:    2 LGASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTY 595
            LGASFETP GR+LIKP+EYM  I S++PN W TLADEVPAWVS+KRNK SVDRTV+WLD+C+ALS   G  FGAIVGGS VEERRR AQE  KRNVS                                           YWIGGFG GES D+RP LL+AVTD LP+  PR I GLGLPEEVLQG+ +G+DLFD++Y
Sbjct:  116 LGASFETPCGRLLIKPAEYMGIISSLRPNLWATLADEVPAWVSKKRNKNSVDRTVKWLDECVALSPASGVAFGAIVGGSDVEERRRCAQEVVKRNVS------------------------------------------GYWIGGFGFGESTDERPALLSAVTDILPKDGPRLISGLGLPEEVLQGVAAGIDLFDSSY 271

[Close]

BLAST of CF810537 vs. TrEMBL
Match: C5WP43|C5WP43_SORBI (Putative uncharacterized protein Sb01g026340 OS=Sorghum bicolor GN=Sb01g026340 PE=4 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 6.392e-55
Identity = 113/214 (52.80%), Postives = 139/214 (64.95%), Query Frame = 2

Query:    5 GASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALS-SKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            GASFETP GR L+KPS+YM+ I  MKPN W +LADEVPAWV++KRNK SV+RT+RWLD CIAL  + G N FG IVGGS +E+R+  A E +KRNVS                                           +WI GFGLGES+++R  LLNAVTD LP +KPR +  LGLPEEVL+G+ +G+DLFD+TYIY LT GGFAL FP++
Sbjct:  119 GASFETPSGRRLVKPSDYMELISCMKPNIWASLADEVPAWVNEKRNKVSVERTLRWLDACIALDVASGTNSFGVIVGGSSLEQRKLCATEVSKRNVS------------------------------------------GFWIAGFGLGESIEERCSLLNAVTDCLPPEKPRLVSRLGLPEEVLEGVAAGIDLFDSTYIYQLTMGGFALIFPVD 290

[Close]

BLAST of CF810537 vs. TrEMBL
Match: C0HHM1|C0HHM1_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 1.424e-54
Identity = 112/214 (52.34%), Postives = 137/214 (64.02%), Query Frame = 2

Query:    5 GASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALS-SKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            GASFETP GR L+KPS+YM+ I  MKPN W +LADEVPAWV++KRNK SVDRT+RWLD CIAL  + G N FG IVGGS +E+R+  A E +KRNVS                                           +WI GFG GES+++R  LLNA TD LP +KPR +  LGLPEEVL+G+ +G+DLFD+TYIY LT GGFAL FP++
Sbjct:  116 GASFETPSGRRLVKPSDYMELISCMKPNIWASLADEVPAWVTEKRNKVSVDRTLRWLDACIALDVASGTNSFGVIVGGSSLEQRKLCATEVSKRNVS------------------------------------------GFWIAGFGFGESIEERCTLLNATTDCLPREKPRLVSRLGLPEEVLEGVAAGIDLFDSTYIYQLTMGGFALIFPVD 287

[Close]

BLAST of CF810537 vs. TrEMBL
Match: Q948C4|Q948C4_ORYSA (Putative tRNA-guanine transglycosylase OS=Oryza sativa GN=OSJNBa0034A02.7 PE=4 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 2.779e-50
Identity = 104/214 (48.60%), Postives = 131/214 (61.21%), Query Frame = 2

Query:    5 GASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIAL-SSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            GASFETP GR L+KPS+YM+ I  MKPN W +LADEVPAWV++KRNK SV+RT+RWLD CIAL    G N  G +VGGS +E R+  A E +KRNVS                                           +WIGG G G+S ++R  +L+A    LP +KPR +  LGLPEEVL+G+ +G+DLFD+TYIY LT GGFAL FP++
Sbjct:  114 GASFETPAGRRLVKPSDYMELISCMKPNLWASLADEVPAWVTEKRNKVSVERTLRWLDACIALDEDSGANTLGVVVGGSSIELRKLCATEVSKRNVS------------------------------------------GFWIGGLGFGDSPEERCSILDAAVGCLPPEKPRVVSRLGLPEEVLEGVAAGIDLFDSTYIYQLTMGGFALIFPID 285

[Close]

BLAST of CF810537 vs. TrEMBL
Match: Q7XGX9|Q7XGX9_ORYSJ (Os10g0141900 protein OS=Oryza sativa subsp. japonica GN=Os10g0141900 PE=4 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 2.779e-50
Identity = 104/214 (48.60%), Postives = 131/214 (61.21%), Query Frame = 2

Query:    5 GASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIAL-SSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            GASFETP GR L+KPS+YM+ I  MKPN W +LADEVPAWV++KRNK SV+RT+RWLD CIAL    G N  G +VGGS +E R+  A E +KRNVS                                           +WIGG G G+S ++R  +L+A    LP +KPR +  LGLPEEVL+G+ +G+DLFD+TYIY LT GGFAL FP++
Sbjct:  114 GASFETPAGRRLVKPSDYMELISCMKPNLWASLADEVPAWVTEKRNKVSVERTLRWLDACIALDEDSGANTLGVVVGGSSIELRKLCATEVSKRNVS------------------------------------------GFWIGGLGFGDSPEERCSILDAAVGCLPPEKPRVVSRLGLPEEVLEGVAAGIDLFDSTYIYQLTMGGFALIFPID 285

[Close]

BLAST of CF810537 vs. TrEMBL
Match: A5AZL4|A5AZL4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_035707 PE=4 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 1.801e-49
Identity = 113/214 (52.80%), Postives = 124/214 (57.94%), Query Frame = 2

Query:    2 LGASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            +GASFETP GR LIKP EYM+ I SMKP+ W TLADEVPAWVS KRNK SVDRT+RWLD+CI LS  GG +FGAIVGG+ VEER+R AQE A RNVS                                           YWIGGFGLGESMD+RP LLNAV D LP +K       G                 + YIYHLT GGFALTFPLE
Sbjct:  113 IGASFETPSGRRLIKPVEYMEMISSMKPSLWATLADEVPAWVSAKRNKASVDRTLRWLDECITLSPAGGAVFGAIVGGANVEERQRCAQEVAGRNVS------------------------------------------GYWIGGFGLGESMDERPALLNAVADILPSRKTTPDLWAG----------------TSRYIYHLTLGGFALTFPLE 268

[Close]

BLAST of CF810537 vs. TrEMBL
Match: C0PRC2|C0PRC2_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.525e-48
Identity = 102/214 (47.66%), Postives = 132/214 (61.68%), Query Frame = 2

Query:    5 GASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGN--IFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPL 640
            GASFETP+G  ++KP +YM+ I ++KPN W++L DEVP+WVS KRN  SVDRT RWLDDC+++   G      GA+VGG+ +E R RSA E A RNV+                                           + + GFGLGES++DRP LL+A+  +LPE KPR I GLGLP EVLQG+ +G+DLFD+TY Y LT GG+A+TFPL
Sbjct:  139 GASFETPYGHRMVKPVQYMENICALKPNLWSSLPDEVPSWVSDKRNIISVDRTTRWLDDCLSMQPTGSESAALGAVVGGTSLELRERSASEVASRNVA------------------------------------------GFLLAGFGLGESIEDRPVLLDAIMRNLPEDKPRHISGLGLPVEVLQGVAAGIDLFDSTYPYLLTMGGYAMTFPL 310

[Close]

BLAST of CF810537 vs. TrEMBL
Match: A9SQW4|A9SQW4_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_232560 PE=4 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.883e-39
Identity = 85/215 (39.53%), Postives = 125/215 (58.14%), Query Frame = 2

Query:    2 LGASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIAL--SSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPL 640
            LGA+FET  GR ++ P++YM+ +++ KP+ W +L DEVP W ++KRN+ SVDRT++WLD C++L  + +G    G +VG + ++ER RSA++ A RNV                                            + +GGFGLGE    R   ++AV   LP++K R   GLGLPEEVLQ +++GVD+FD+ Y + LT GG+A+TFPL
Sbjct:   94 LGAAFETTSGRRVVGPAKYMEIVNACKPDLWVSLPDEVPTWAAEKRNRLSVDRTLQWLDHCLSLQPAERGNRCLGVVVGSASLDERIRSAEQTATRNV------------------------------------------VGFSLGGFGLGEDASQRALQMDAVIAKLPKEKLRHTSGLGLPEEVLQAVEAGVDVFDSIYPFMLTKGGYAMTFPL 266

[Close]

BLAST of CF810537 vs. Vitis vinifera peptide
Match: GSVIVT01036437001 (assembled CDS)

HSP 1 Score: 255.373 bits (651), Expect = 1.342e-68
Identity = 132/214 (61.68%), Postives = 147/214 (68.69%), Query Frame = 2

Query:    2 LGASFETPFGRILIKPSEYMKFIDSMKPNSWTTLADEVPAWVSQKRNKTSVDRTVRWLDDCIALSSKGGNIFGAIVGGSCVEERRRSAQEAAKRNVSDEASWSIALRRLVFPFIHTTNALSLFGMSPWSILLSHNSPMTSYWIGGFGLGESMDDRPPLLNAVTDSLPEQKPRQICGLGLPEEVLQGIDSGVDLFDTTYIYHLTTGGFALTFPLE 643
            +GASFETP GR LIKP EYM+ I SMKP+ W TLADEVPAWVS KRNK SVDRT+RWLD+CI LS  GG +FGAIVGG+ VEER+R AQE A RNVS                                           YWIGGFGLGESMD+RP LLNAV D LP ++PRQICGL LPEEVLQG+ +G+DLFD+TYIYHLT GGFALTFPLE
Sbjct:  113 IGASFETPSGRRLIKPVEYMEMISSMKPSLWATLADEVPAWVSAKRNKASVDRTLRWLDECITLSPAGGAVFGAIVGGANVEERQRCAQEVAGRNVS------------------------------------------GYWIGGFGLGESMDERPALLNAVADILPVERPRQICGLELPEEVLQGVAAGIDLFDSTYIYHLTLGGFALTFPLE 284

The following BLAST results are available for this feature:

BLAST of CF810537 vs. DB:swissprot:display
BLAST of CF810537 vs. Populus trichocarpa peptide v2.0
BLAST of CF810537 vs. P. persica 1.0 peptide
BLAST of CF810537 vs. TrEMBL
BLAST of CF810537 vs. Vitis vinifera peptide

BLAST of CF810537 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
QTRD1_XENTR	3.303e-19	33.49	Queuine tRNA-ribosyltransferase subunit qtrtd1 OS=...	[more]
QTRD1_MOUSE	9.609e-19	31.78	Queuine tRNA-ribosyltransferase subunit QTRTD1 OS=...	[more]
QTRD1_CHICK	9.609e-19	31.43	Queuine tRNA-ribosyltransferase subunit QTRTD1 OS=...	[more]
QTRD1_XENLA	4.769e-18	31.90	Queuine tRNA-ribosyltransferase subunit qtrtd1 OS=...	[more]
QTRD1_PONAB	4.769e-18	31.31	Queuine tRNA-ribosyltransferase subunit QTRTD1 OS=...	[more]
QTRD1_HUMAN	8.134e-18	31.31	Queuine tRNA-ribosyltransferase subunit QTRTD1 OS=...	[more]
QTRD1_DANRE	1.812e-17	29.05	Queuine tRNA-ribosyltransferase subunit qtrtd1 OS=...	[more]
QTRD1_TRIAD	2.708e-13	29.44	Queuine tRNA-ribosyltransferase subunit QTRTD1 hom...	[more]
TGT_DEIRA	2.292e-12	28.41	Queuine tRNA-ribosyltransferase OS=Deinococcus rad...	[more]
TGT_XENTR	2.535e-11	25.47	Queuine tRNA-ribosyltransferase OS=Xenopus tropica...	[more]

BLAST of CF810537 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
POPTR_0019s10480.1	3.545e-66	59.81		[more]

BLAST of CF810537 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ppa023702m	1.919e-66	58.69		[more]

BLAST of CF810537 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
D7TLI8\|D7TLI8_VITVI	2.766e-66	61.68	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6T7F2\|C6T7F2_SOYBN	2.192e-63	56.74	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9IQC4\|B9IQC4_POPTR	3.747e-55	57.58	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5WP43\|C5WP43_SORBI	6.392e-55	52.80	Putative uncharacterized protein Sb01g026340 OS=So...	[more]
C0HHM1\|C0HHM1_MAIZE	1.424e-54	52.34	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q948C4\|Q948C4_ORYSA	2.779e-50	48.60	Putative tRNA-guanine transglycosylase OS=Oryza sa...	[more]
Q7XGX9\|Q7XGX9_ORYSJ	2.779e-50	48.60	Os10g0141900 protein OS=Oryza sativa subsp. japoni...	[more]
A5AZL4\|A5AZL4_VITVI	1.801e-49	52.80	Putative uncharacterized protein OS=Vitis vinifera...	[more]
C0PRC2\|C0PRC2_PICSI	1.525e-48	47.66	Putative uncharacterized protein OS=Picea sitchens...	[more]
A9SQW4\|A9SQW4_PHYPA	2.883e-39	39.53	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of CF810537 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1


Match Name	E-value	Identity	Description
GSVIVT01036437001	1.342e-68	61.68	assembled CDS	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002616	Queuine/other tRNA-ribosyltransferase	GENE3D	3.20.20.105	no description	coord: 128..213 score: 2.9
IPR002616	Queuine/other tRNA-ribosyltransferase	PANTHER	PTHR11962	QUEUINE TRNA-RIBOSYLTRANSFERASE	coord: 2..211 score: 7
IPR002616	Queuine/other tRNA-ribosyltransferase	PFAM	PF01702	TGT	coord: 18..93 score: 1.7e-08coord: 137..211 score: 8.1
IPR002616	Queuine/other tRNA-ribosyltransferase	SUPERFAMILY	51713	tRNA-guanine transglycosylase	coord: 141..213 score: 1.7e-15coord: 2..97 score: 1.4
None	No IPR available	SEG	seg	seg	coord: 1..11 score

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF810537 ID=CF810537; Name=CF810537; organism=Vaccinium corymbosum; type=EST; length=648bp
GTTAGGAGCATCTTTTGAAACTCCCTTTGGTCGCATCTTGATAAAACCTT
CAGAGTACATGAAATTTATTGATTCTATGAAGCCAAATTCATGGACCACT
TTAGCTGATGAAGTACCTGCATGGGTTTCTCAGAAGAGGAACAAAACCTC
AGTAGACCGAACCGTGAGGTGGCTTGATGATTGCATTGCGTTAAGCTCGA
AAGGTGGGAATATTTTTGGTGCCATTGTTGGAGGCTCTTGTGTGGAAGAA
CGGCGGCGAAGTGCTCAGGAAGCAGCTAAGCGAAACGTATCAGACGAAGC
AAGTTGGTCAATTGCTCTTCGAAGGCTGGTGTTTCCTTTCATACACACCA
CAAATGCTCTATCTCTCTTTGGCATGTCTCCATGGTCAATCTTGTTGTCC
CACAATTCTCCGATGACTAGTTACTGGATTGGTGGGTTTGGGCTTGGTGA
GAGCATGGATGATCGCCCTCCTCTCCTTAATGCTGTTACGGATAGTTTAC
CGGAGCAAAAGCCACGTCAGATTTGTGGCCTTGGACTTCCAGAGGAGGTC
TTGCAGGGAATCGACTCAGGAGTTGATCTTTTTGACACCACGTATATTTA
CCATCTTACAACAGGAGGCTTTGCGCTTACTTTTCCGCTGGAAAAANT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006400	tRNA modification
GO:0008616	queuosine biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008479	tRNA-guanosine(34) queuine transglycosylase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR002616	tRNA_ribo_trans-like
noIPR	unintegrated