CF810638

EST Overview
NameCF810638
Unique NameCF810638
TypeEST
OrganismVaccinium corymbosum (northern highbush blueberry)
Sequence length426
Libraries
Library NameType
LIBEST_014530 cDNA cold acclimated Bluecrop librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Vaccinium corymbosum unigene v1EST
Homology
[Close]
BLAST of CF810638 vs. DB:swissprot:display
Match: PME31_ARATH (Pectinesterase 31 OS=Arabidopsis thaliana GN=PME31 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 4.823e-21
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSC S+R  W+REL+D+EA  F+ HSF+DP+  RPWL  RM V+ PYS
Sbjct:  255 ERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQDRPWLCLRMGVKTPYS 316
[Close]
BLAST of CF810638 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0023s00200.1 ()

HSP 1 Score: 120.553 bits (301), Expect = 3.695e-28
Identity = 51/62 (82.26%), Postives = 57/62 (91.94%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSCPSKR TW+REL+DEEA+QFLMH FIDPD +RPWL QRMA+ IPYS
Sbjct:  254 ERSACFYEYRCFGPGSCPSKRVTWSRELIDEEADQFLMHCFIDPDPERPWLCQRMALSIPYS 315
[Close]
BLAST of CF810638 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0004s13310.1 ()

HSP 1 Score: 120.553 bits (301), Expect = 3.695e-28
Identity = 51/62 (82.26%), Postives = 57/62 (91.94%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSCPSKR TW+REL+DEEA+QFLMH FIDPD +RPWL QRMA+ IPYS
Sbjct:  254 ERSACFYEYRCFGPGSCPSKRVTWSRELIDEEADQFLMHCFIDPDPERPWLCQRMALSIPYS 315
[Close]
BLAST of CF810638 vs. P. persica 1.0 peptide
Match: ppa008873m ()

HSP 1 Score: 121.709 bits (304), Expect = 1.197e-28
Identity = 53/62 (85.48%), Postives = 57/62 (91.94%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSCPSKR TWAREL+DEEA QFL H FIDPD +RPWLAQRMA+RIPYS
Sbjct:  254 ERSACFYEYRCFGPGSCPSKRVTWARELLDEEAVQFLQHGFIDPDPERPWLAQRMALRIPYS 315
[Close]
BLAST of CF810638 vs. TrEMBL
Match: D7SXF6|D7SXF6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_108.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011459001 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 8.813e-29
Identity = 57/62 (91.94%), Postives = 60/62 (96.77%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSCPSKR TWARELVDEEAEQFLMHSFIDPD +RPWLAQRMA+RIPYS
Sbjct:  254 ERSACFYEYRCFGPGSCPSKRVTWARELVDEEAEQFLMHSFIDPDVERPWLAQRMALRIPYS 315
[Close]
BLAST of CF810638 vs. TrEMBL
Match: B9SVI9|B9SVI9_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_0537800 PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 7.460e-28
Identity = 54/62 (87.10%), Postives = 59/62 (95.16%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSCPSKR TWAREL+DEEAEQFL+H FIDPD QRPWLAQRMA+RIPY+
Sbjct:  254 ERSACFYEYRCFGPGSCPSKRVTWARELIDEEAEQFLVHGFIDPDAQRPWLAQRMALRIPYT 315
[Close]
BLAST of CF810638 vs. TrEMBL
Match: B9H3W4|B9H3W4_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTRDRAFT_818003 PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.346e-26
Identity = 51/62 (82.26%), Postives = 57/62 (91.94%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSCPSKR TW+REL+DEEA+QFLMH FIDPD +RPWL QRMA+ IPYS
Sbjct:  254 ERSACFYEYRCFGPGSCPSKRVTWSRELIDEEADQFLMHCFIDPDPERPWLCQRMALSIPYS 315
[Close]
BLAST of CF810638 vs. TrEMBL
Match: A2Q3R5|A2Q3R5_MEDTR (Pectinesterase OS=Medicago truncatula GN=MtrDRAFT_AC155888g12v2 PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.536e-24
Identity = 49/62 (79.03%), Postives = 53/62 (85.48%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPG CPS RA W RELVDEEAEQF+MH FIDP+  R WLAQRM +RIPYS
Sbjct:  254 ERSACFYEYRCFGPGCCPSNRANWCRELVDEEAEQFIMHPFIDPEPDRSWLAQRMGLRIPYS 315
[Close]
BLAST of CF810638 vs. TrEMBL
Match: C5WM71|C5WM71_SORBI (Putative uncharacterized protein Sb01g022290 OS=Sorghum bicolor GN=Sb01g022290 PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.763e-21
Identity = 45/62 (72.58%), Postives = 50/62 (80.65%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ERTACFYEYRC GPGS PS R TW R+L+D EAEQFL H+FIDPD  RPWL Q MA+R+P S
Sbjct:  319 ERTACFYEYRCSGPGSQPSNRVTWCRQLLDVEAEQFLAHTFIDPDVDRPWLLQMMAIRVPAS 380
[Close]
BLAST of CF810638 vs. TrEMBL
Match: B6TZE6|B6TZE6_MAIZE (Pectinesterase-1 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.763e-21
Identity = 45/62 (72.58%), Postives = 50/62 (80.65%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ERTACFYEYRC GPG+ PS R TW R+L+D EAEQFL H+FIDPD  RPWL Q MA+RIP S
Sbjct:  262 ERTACFYEYRCSGPGALPSNRVTWCRQLLDVEAEQFLAHTFIDPDVDRPWLLQMMAIRIPAS 323
[Close]
BLAST of CF810638 vs. TrEMBL
Match: D7LNT6|D7LNT6_ARALY (Pectinesterase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_905199 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.384e-20
Identity = 42/62 (67.74%), Postives = 50/62 (80.65%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSC S+R  W+REL+DEEA  F+ HSF+DP+  RPWL  RM V+ PYS
Sbjct:  255 ERSACFYEYRCFGPGSCSSERVPWSRELMDEEAGHFVHHSFVDPEQDRPWLCLRMGVKTPYS 316
[Close]
BLAST of CF810638 vs. TrEMBL
Match: Q948H9|Q948H9_ORYSA (Putative pectin methylesterase OS=Oryza sativa GN=OSJNBa0060A14.17 PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.142e-18
Identity = 40/62 (64.52%), Postives = 46/62 (74.19%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ERTACF+EYRC GPG  PS R  W R+L+D E E FL HSFIDPD  RPWL Q MA+++P S
Sbjct:  274 ERTACFFEYRCSGPGFRPSNRVAWCRQLLDVEVENFLSHSFIDPDLDRPWLIQMMAIKVPVS 335
[Close]
BLAST of CF810638 vs. TrEMBL
Match: Q7XEU2|Q7XEU2_ORYSJ (Os10g0407000 protein OS=Oryza sativa subsp. japonica GN=Os10g0407000 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.142e-18
Identity = 40/62 (64.52%), Postives = 46/62 (74.19%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ERTACF+EYRC GPG  PS R  W R+L+D E E FL HSFIDPD  RPWL Q MA+++P S
Sbjct:  274 ERTACFFEYRCSGPGFRPSNRVAWCRQLLDVEVENFLSHSFIDPDLDRPWLIQMMAIKVPVS 335
[Close]
BLAST of CF810638 vs. TrEMBL
Match: A6N0X1|A6N0X1_ORYSI (Pectinesterase-1 (Fragment) OS=Oryza sativa subsp. indica PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.142e-18
Identity = 40/62 (64.52%), Postives = 46/62 (74.19%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ERTACF+EYRC GPG  PS R  W R+L+D E E FL HSFIDPD  RPWL Q MA+++P S
Sbjct:  134 ERTACFFEYRCSGPGFRPSNRVAWCRQLLDVEVENFLSHSFIDPDLDRPWLIQMMAIKVPVS 195
[Close]
BLAST of CF810638 vs. Vitis vinifera peptide
Match: GSVIVT01011459001 (assembled CDS)

HSP 1 Score: 129.798 bits (325), Expect = 3.803e-31
Identity = 57/62 (91.94%), Postives = 60/62 (96.77%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSCPSKR TWARELVDEEAEQFLMHSFIDPD +RPWLAQRMA+RIPYS
Sbjct:  254 ERSACFYEYRCFGPGSCPSKRVTWARELVDEEAEQFLMHSFIDPDVERPWLAQRMALRIPYS 315
[Close]
BLAST of CF810638 vs. TAIR peptide
Match: AT3G29090.1 (| Symbols: PME31, ATPME31 | pectin methylesterase 31 | chr3:11073804-11075335 FORWARD LENGTH=317)

HSP 1 Score: 99.7525 bits (247), Expect = 5.996e-22
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 2 
Query:    2 ERTACFYEYRCFGPGSCPSKRATWARELVDEEAEQFLMHSFIDPDCQRPWLAQRMAVRIPYS 187
            ER+ACFYEYRCFGPGSC S+R  W+REL+D+EA  F+ HSF+DP+  RPWL  RM V+ PYS
Sbjct:  255 ERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQDRPWLCLRMGVKTPYS 316
The following BLAST results are available for this feature:
BLAST of CF810638 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 1
Match NameE-valueIdentityDescription
PME31_ARATH4.823e-2166.13Pectinesterase 31 OS=Arabidopsis thaliana GN=PME31... [more]
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BLAST of CF810638 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
POPTR_0023s00200.13.695e-2882.26[more]
POPTR_0004s13310.13.695e-2882.26[more]
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BLAST of CF810638 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
ppa008873m1.197e-2885.48[more]
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BLAST of CF810638 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10
Match NameE-valueIdentityDescription
D7SXF6|D7SXF6_VITVI8.813e-2991.94Whole genome shotgun sequence of line PN40024, sca... [more]
B9SVI9|B9SVI9_RICCO7.460e-2887.10Pectinesterase OS=Ricinus communis GN=RCOM_0537800... [more]
B9H3W4|B9H3W4_POPTR5.346e-2682.26Pectinesterase OS=Populus trichocarpa GN=POPTRDRAF... [more]
A2Q3R5|A2Q3R5_MEDTR6.536e-2479.03Pectinesterase OS=Medicago truncatula GN=MtrDRAFT_... [more]
C5WM71|C5WM71_SORBI6.763e-2172.58Putative uncharacterized protein Sb01g022290 OS=So... [more]
B6TZE6|B6TZE6_MAIZE6.763e-2172.58Pectinesterase-1 OS=Zea mays PE=2 SV=1[more]
D7LNT6|D7LNT6_ARALY4.384e-2067.74Pectinesterase family protein OS=Arabidopsis lyrat... [more]
Q948H9|Q948H9_ORYSA3.142e-1864.52Putative pectin methylesterase OS=Oryza sativa GN=... [more]
Q7XEU2|Q7XEU2_ORYSJ3.142e-1864.52Os10g0407000 protein OS=Oryza sativa subsp. japoni... [more]
A6N0X1|A6N0X1_ORYSI3.142e-1864.52Pectinesterase-1 (Fragment) OS=Oryza sativa subsp.... [more]
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BLAST of CF810638 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1
Match NameE-valueIdentityDescription
GSVIVT010114590013.803e-3191.94assembled CDS[more]
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BLAST of CF810638 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
AT3G29090.15.996e-2266.13| Symbols: PME31, ATPME31 | pectin methylesterase ... [more]
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InterPro
Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10no descriptioncoord: 2..52
score: 7.8

Properties
Property NameValue
Singlet in analysisVaccinium corymbosum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>CF810638 ID=CF810638; Name=CF810638; organism=Vaccinium corymbosum; type=EST; length=426bp
AGAGAGAACTGCTTGCTTTTACGAGTACAGGTGTTTTGGGCCTGGTAGCT
GTCCATCAAAACGGGCGACCTGGGCAAGGGAACTAGTAGATGAAGAAGCA
GAACAGTTCCTCATGCACTCTTTCATCGATCCAGATTGCCAAAGGCCTTG
GCTAGCTCAGAGAATGGCCGTGAGGATTCCATATTCGGTCTAGGAATTAG
TCGAAGGCATAAGGTCAGACGCAGGAGATTCCCCACTTATAATAATCGAA
ACGAGGTATCNTATTAGGCTTTCATGTTAGATTCAATAATGCTTCCTGGA
TGGGTGGCAAATAAGTGACTCCTGCCGATGCAGATACTGTGTATTTAATC
TACAAAAAGCTATGATATCTGCTTAGCAGTGTATTTTATGTATTTCGAAG
CAGCTAAATTGTTCTCTGGAGAAAAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold