CV090326 | GDV

EST Overview


Name	CV090326
Unique Name	CV090326
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	703

Libraries


Library Name	Type
LIBEST_014530 cDNA cold acclimated Bluecrop library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18
KEGG analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

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BLAST of CV090326 vs. DB:swissprot:display
Match: IF4G1_ARATH (Eukaryotic translation initiation factor 4G OS=Arabidopsis thaliana GN=EIF4G PE=1 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 2.013e-52
Identity = 119/242 (49.17%), Postives = 157/242 (64.88%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAP--GPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSL-HEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSP------EDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFIS 701
            TLGPQGGL +GMSIR     SN   +DA   G GDSRR A GLNG+ S                   R A  + H +SS Q+R    V+R+      +SD S PE  + R    G +  V+SP      E++L ++S++ IKE+YSA+DE E+ +C+K++NSP+++P+MIS+WV DSFERKD ERDLLAKLL+NL K+ D  LN+  L++GFESVL TLEDA+NDAPKA EFLGRIF K ++
Sbjct: 1425 TLGPQGGLGQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGS------------------HRPASPVTHGRSSPQERGTAYVHREFASLSRASDLS-PEVSSARQVLQGPSATVNSPRENALSEEQLENLSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLVNLVKSADNALNEVQLVKGFESVLKTLEDAVNDAPKAAEFLGRIFGKSVT 1647

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BLAST of CV090326 vs. DB:swissprot:display
Match: Y6969_DICDI (von Willebrand factor A domain-containing protein DDB_G0286969 OS=Dictyostelium discoideum GN=DDB_G0286969 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 6.132e-9
Identity = 40/142 (28.17%), Postives = 68/142 (47.89%), Query Frame = 3

Query:  258 NRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSPEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRI 683
            +RD+R      +R  P  P    +      N    ED   D+SM T+ E+   +D  E   CIKE+N P+      +I++  S ERK+ E+ L+ +L   +   +  + N + +  G + ++ T+ED   D P +G F+  I
Sbjct: 1727 DRDSRGPSKPDNRKQPAQPPAPVEPVKPKINFEKIED---DISM-TLDEYAETQDIDEAIECIKEINEPTVLGKQFNIFIMKSLERKEKEKQLIIELFSGILTAQ--LFNAEQIKEGLKEIIDTIEDIEIDLPFSGVFVATI 1862

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BLAST of CV090326 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0002s14110.1 ()

HSP 1 Score: 294.278 bits (752), Expect = 4.917e-80
Identity = 152/239 (63.60%), Postives = 189/239 (79.08%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLAD-APGPGDSRRIAAGLNGYNSVSERTVYG-REDLMPRYIPERFA-GSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSS----PEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRGQP+     +AD +P PGD RR+AAGLNG +++S R+ Y  RED++PRY P+RFA     +Q + Q+RNMN VNRD R  DH  DR L  SP  R QGP F+Q   +    PE+RLRDMS A IKEFYSA+DEKEV+LCIKELNSPSF+PSMISIWV DSFERKD+ERDLLAKLL++L+++++G+L+ + LI+GFES+L+TLEDA+NDAPKA EFLGRI  + +
Sbjct: 1579 TLGPQGGLARGMSIRGQPASMGTLVADISPSPGDPRRMAAGLNGSSAISGRSNYSPREDIIPRYTPDRFAVPPACDQMNGQERNMNYVNRDLRNLDHGFDRPLGSSPPTRAQGPPFSQTTPTGKLWPEERLRDMSTAAIKEFYSARDEKEVSLCIKELNSPSFHPSMISIWVTDSFERKDLERDLLAKLLVSLARSQNGILDSNQLIKGFESILTTLEDAVNDAPKAPEFLGRIIGRVV 1817

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BLAST of CV090326 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0014s05150.1 ()

HSP 1 Score: 292.352 bits (747), Expect = 1.868e-79
Identity = 150/239 (62.76%), Postives = 190/239 (79.50%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLAD-APGPGDSRRIAAGLNGYNSVSERTVYG-REDLMPRYIPERFA-GSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSS----PEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRG P+I+  P+++ +P P DSRR+AAGLNG +++ ER+ Y  REDL+PRY P+RFA    H+Q S Q+RNMN VNRD R  DH  DR L  S     QGP FAQ++ +    PE++LR+MSM TIKEFYSA+DEKEVALCIK+LNSPSF+PSMIS+WV DSFERKDM+RDLLAKLL +L++++D +L+ + L++GFESVL+TLEDA+ DAPKA EFLGRI  + +
Sbjct: 1605 TLGPQGGLARGMSIRGTPAITVAPVSEISPSPSDSRRMAAGLNGVSAILERSNYSPREDLIPRYSPDRFAVPPTHDQMSGQERNMNYVNRDLRNLDHGFDRPLGSSSLTNTQGPSFAQSIPTGKMWPEEQLREMSMVTIKEFYSARDEKEVALCIKDLNSPSFHPSMISLWVTDSFERKDMDRDLLAKLLASLTRSQDCILDSNQLVKGFESVLTTLEDAVTDAPKAPEFLGRILGRVV 1843

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BLAST of CV090326 vs. P. persica 1.0 peptide
Match: ppa000085m ()

HSP 1 Score: 298.13 bits (762), Expect = 2.403e-81
Identity = 157/239 (65.69%), Postives = 186/239 (77.82%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLAD-APGPGDSRRIAAGLNGYNSVSERTVYG-REDLMPRYIPERFAG-SLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSP----EDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRG PS+S  P A+ +P  GDSRR+ AGLNG++S+SER  Y  R++ MPR++P+RFAG + ++QS+  +RN+N   RD R  D S DRS P SP  R   P   QNV       EDRLRDMS+A IKEFYSA+DEKEV LCIKELNSPSF+PSMIS+WV DSFERKD ERDLLAKLL+NL+K+ DG L+Q  LI+GFE+VLSTLEDA+NDAPKA EFLG IFAK I
Sbjct: 1551 TLGPQGGLARGMSIRGPPSMSAAPHAELSPSVGDSRRMTAGLNGFSSLSERPTYNPRDEHMPRHLPDRFAGPAAYDQSNAPERNVNFGGRDPRNLDRSFDRSRPASPATRAHAPALTQNVPQEKVLTEDRLRDMSLAAIKEFYSARDEKEVVLCIKELNSPSFHPSMISLWVTDSFERKDTERDLLAKLLVNLTKSHDGTLSQSQLIKGFETVLSTLEDAVNDAPKAPEFLGLIFAKVI 1789

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BLAST of CV090326 vs. TrEMBL
Match: B9MV40|B9MV40_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_677642 PE=4 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 2.406e-77
Identity = 150/239 (62.76%), Postives = 190/239 (79.50%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLAD-APGPGDSRRIAAGLNGYNSVSERTVYG-REDLMPRYIPERFA-GSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSS----PEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRG P+I+  P+++ +P P DSRR+AAGLNG +++ ER+ Y  REDL+PRY P+RFA    H+Q S Q+RNMN VNRD R  DH  DR L  S     QGP FAQ++ +    PE++LR+MSM TIKEFYSA+DEKEVALCIK+LNSPSF+PSMIS+WV DSFERKDM+RDLLAKLL +L++++D +L+ + L++GFESVL+TLEDA+ DAPKA EFLGRI  + +
Sbjct:  849 TLGPQGGLARGMSIRGTPAITVAPVSEISPSPSDSRRMAAGLNGVSAILERSNYSPREDLIPRYSPDRFAVPPTHDQMSGQERNMNYVNRDLRNLDHGFDRPLGSSSLTNTQGPSFAQSIPTGKMWPEEQLREMSMVTIKEFYSARDEKEVALCIKDLNSPSFHPSMISLWVTDSFERKDMDRDLLAKLLASLTRSQDCILDSNQLVKGFESVLTTLEDAVTDAPKAPEFLGRILGRVV 1087

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BLAST of CV090326 vs. TrEMBL
Match: A9PHK2|A9PHK2_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 2.406e-77
Identity = 150/239 (62.76%), Postives = 190/239 (79.50%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLAD-APGPGDSRRIAAGLNGYNSVSERTVYG-REDLMPRYIPERFA-GSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSS----PEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRG P+I+  P+++ +P P DSRR+AAGLNG +++ ER+ Y  REDL+PRY P+RFA    H+Q S Q+RNMN VNRD R  DH  DR L  S     QGP FAQ++ +    PE++LR+MSM TIKEFYSA+DEKEVALCIK+LNSPSF+PSMIS+WV DSFERKDM+RDLLAKLL +L++++D +L+ + L++GFESVL+TLEDA+ DAPKA EFLGRI  + +
Sbjct:  353 TLGPQGGLARGMSIRGTPAITVAPVSEISPSPSDSRRMAAGLNGVSAILERSNYSPREDLIPRYSPDRFAVPPTHDQMSGQERNMNYVNRDLRNLDHGFDRPLGSSSLTNTQGPSFAQSIPTGKMWPEEQLREMSMVTIKEFYSARDEKEVALCIKDLNSPSFHPSMISLWVTDSFERKDMDRDLLAKLLASLTRSQDCILDSNQLVKGFESVLTTLEDAVTDAPKAPEFLGRILGRVV 591

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BLAST of CV090326 vs. TrEMBL
Match: B9SKF9|B9SKF9_RICCO (Eukaryotic translation initiation factor 4g, putative OS=Ricinus communis GN=RCOM_0658590 PE=4 SV=1)

HSP 1 Score: 281.952 bits (720), Expect = 3.251e-74
Identity = 155/240 (64.58%), Postives = 187/240 (77.92%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLAD-APGPGDSRRIAAGLNGYNSVSERTVYG-REDLMPRYIPERFA-GSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSS----PEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDG-MLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMS RG P+++  P+AD +P  GD RR+AAGLNG+++VSER  Y  RE+  PRY P+RFA  +  +QSS  +RNMN VNRD R  D + DRS   SP  R Q P F QN+ S    PE+RLRDMSMA IKEFYSA+DEKEVALCIKEL++ SF+PSMIS+WV DSFERKDMERDLLAKLLINL++++D  +L    LI+GFESVL+TLEDA+NDAPKA EFLGR+ AK +
Sbjct: 1437 TLGPQGGLARGMSFRGPPAMAGGPIADISPSSGD-RRMAAGLNGFSTVSERPAYSPREEFFPRY-PDRFALPAAFDQSSGHERNMNYVNRDPRNQDRNFDRSHATSPPGRAQLPAFTQNIPSEKVWPEERLRDMSMAAIKEFYSARDEKEVALCIKELSASSFHPSMISLWVTDSFERKDMERDLLAKLLINLARSQDDRILTSSQLIKGFESVLTTLEDAVNDAPKAAEFLGRMLAKAV 1674

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BLAST of CV090326 vs. TrEMBL
Match: C5XBH3|C5XBH3_SORBI (Putative uncharacterized protein Sb02g036110 OS=Sorghum bicolor GN=Sb02g036110 PE=4 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 7.792e-52
Identity = 116/233 (49.79%), Postives = 150/233 (64.38%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAPGPGDSRRIAAGLNGYNSVSERTVY-GREDLMPRYIPERFAGSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSPEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRGQP +SN  L   P   D RRI +G NGYNS ++ T   GRED   R IP+R +G     S     +  P +++ R    S                         ED LR+ S++ I+E+YSAKDEKEVALCI ELN+PSFYPS++S+WVNDSFERKDMER+LLAKLL++L   R  +L++  L  G  SVL++LED ++DAP+A E+LGR+ A+F+
Sbjct:  542 TLGPQGGLARGMSIRGQPPVSNTEL---PSVIDHRRIVSGSNGYNSAADWTSSSGREDSSSR-IPDRTSGRTPASSQSAGPSQRPASQEGRSGSKSYS-----------------------EDELREKSVSAIREYYSAKDEKEVALCIVELNAPSFYPSVVSLWVNDSFERKDMERELLAKLLVSLCSGRHNLLSKQQLSDGLSSVLASLEDTLSDAPRATEYLGRLLARFV 747

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BLAST of CV090326 vs. TrEMBL
Match: Q0WVG9|Q0WVG9_ARATH (Initiation factor eIF-4 gamma like OS=Arabidopsis thaliana GN=At3g60235 PE=2 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 2.961e-51
Identity = 119/242 (49.17%), Postives = 157/242 (64.88%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAP--GPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSL-HEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSP------EDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFIS 701
            TLGPQGGL +GMSIR     SN   +DA   G GDSRR A GLNG+ S                   R A  + H +SS Q+R    V+R+      +SD S PE  + R    G +  V+SP      E++L ++S++ IKE+YSA+DE E+ +C+K++NSP+++P+MIS+WV DSFERKD ERDLLAKLL+NL K+ D  LN+  L++GFESVL TLEDA+NDAPKA EFLGRIF K ++
Sbjct: 1427 TLGPQGGLGQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGS------------------HRPASPVTHGRSSPQERGTAYVHREFASLSRASDLS-PEVSSARQVLQGPSATVNSPRENALSEEQLENLSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLVNLVKSADNALNEVQLVKGFESVLKTLEDAVNDAPKAAEFLGRIFGKSVT 1649

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BLAST of CV090326 vs. TrEMBL
Match: A8MR97|A8MR97_ARATH (Uncharacterized protein At3g60240.2 OS=Arabidopsis thaliana GN=At3g60240 PE=4 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 2.961e-51
Identity = 119/242 (49.17%), Postives = 157/242 (64.88%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAP--GPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSL-HEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSP------EDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFIS 701
            TLGPQGGL +GMSIR     SN   +DA   G GDSRR A GLNG+ S                   R A  + H +SS Q+R    V+R+      +SD S PE  + R    G +  V+SP      E++L ++S++ IKE+YSA+DE E+ +C+K++NSP+++P+MIS+WV DSFERKD ERDLLAKLL+NL K+ D  LN+  L++GFESVL TLEDA+NDAPKA EFLGRIF K ++
Sbjct: 1423 TLGPQGGLGQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGS------------------HRPASPVTHGRSSPQERGTAYVHREFASLSRASDLS-PEVSSARQVLQGPSATVNSPRENALSEEQLENLSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLVNLVKSADNALNEVQLVKGFESVLKTLEDAVNDAPKAAEFLGRIFGKSVT 1645

[Close]

BLAST of CV090326 vs. TrEMBL
Match: D4N2J6|D4N2J6_ORYSI (Eukaryotic translation initiation factor 4g (Fragment) OS=Oryza sativa subsp. indica PE=4 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 8.615e-51
Identity = 118/232 (50.86%), Postives = 156/232 (67.24%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAPGPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSPEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRGQP ISN  L+ A    DSRR+ +G NGYNS S      RE+   R IP+R        S R       +  +T++A  S+  +  E         G + N    ED LR+ S++ I+E+YSAKDEKEVALCI+ELN+PSFYPS++S+WVNDSFERKDMER+LL KL ++L  TR+ +L++ HL  G  +VL +LEDA++DAP+A E+LGR+ A+F+
Sbjct: 1222 TLGPQGGLARGMSIRGQPLISNAELSSA----DSRRMVSGPNGYNSAST----AREEPGSR-IPDR--------SGR-------IAPNTQFAGPSNRPASQE---------GRSGNKLYSEDDLREKSISAIREYYSAKDEKEVALCIEELNAPSFYPSVVSLWVNDSFERKDMERELLTKLFVSLCNTRNNLLSKSHLTAGLATVLGSLEDALSDAPRAAEYLGRLLARFV 1420

[Close]

BLAST of CV090326 vs. TrEMBL
Match: D4N2J5|D4N2J5_ORYSI (Eukaryotic translation initiation factor 4g (Fragment) OS=Oryza sativa subsp. indica PE=4 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 8.615e-51
Identity = 118/232 (50.86%), Postives = 156/232 (67.24%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAPGPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSPEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRGQP ISN  L+ A    DSRR+ +G NGYNS S      RE+   R IP+R        S R       +  +T++A  S+  +  E         G + N    ED LR+ S++ I+E+YSAKDEKEVALCI+ELN+PSFYPS++S+WVNDSFERKDMER+LL KL ++L  TR+ +L++ HL  G  +VL +LEDA++DAP+A E+LGR+ A+F+
Sbjct: 1222 TLGPQGGLARGMSIRGQPLISNAELSSA----DSRRMVSGPNGYNSAST----AREEPGSR-IPDR--------SGR-------IAPNTQFAGPSNRPASQE---------GRSGNKLYSEDDLREKSISAIREYYSAKDEKEVALCIEELNAPSFYPSVVSLWVNDSFERKDMERELLTKLFVSLCNTRNNLLSKSHLTAGLATVLGSLEDALSDAPRAAEYLGRLLARFV 1420

[Close]

BLAST of CV090326 vs. TrEMBL
Match: D7LRN0|D7LRN0_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_907545 PE=4 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 1.470e-50
Identity = 119/242 (49.17%), Postives = 156/242 (64.46%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAP--GPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSL-HEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSP------EDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFIS 701
            TLGPQGGL +GMSIR    +SN   +DA   G GDSRR A GLN + S                   R A  + H +SS Q+R    V+RD      +SD S PE  + R    G +  V+SP      E+RL +MS++ IKE+YSA+DE E+ +C+K++NSP+++P+MIS+WV DSFERKD ERDLLAKLL+NL K+ D  L +  L++GFESVL+TLEDA+NDAPKA EFLGRI  K ++
Sbjct: 1433 TLGPQGGLGQGMSIRRPAVVSNTFQSDATQAGGGDSRRPAGGLNVFGS------------------HRPASPVTHGRSSPQERGTAYVHRDFASLSRTSDPS-PEVSSARQVLQGPSSTVNSPRENALSEERLHNMSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLVNLVKSADNALTEVQLVKGFESVLTTLEDAVNDAPKAAEFLGRIVGKSVT 1655

[Close]

BLAST of CV090326 vs. TrEMBL
Match: Q69S49|Q69S49_ORYSJ (Putative eukaryotic translation initiation factor 4G OS=Oryza sativa subsp. japonica GN=OSJNBa0058I18.6 PE=4 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 2.507e-50
Identity = 117/232 (50.43%), Postives = 156/232 (67.24%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAPGPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSLHEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSPEDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            TLGPQGGLARGMSIRGQP ISN  L+ A    DSRR+ +G NGYNS S      RE+   R IP+R        S R       +  +T++A  S+  +  E         G + N    ED LR+ S++ I+E+YSAKDEKEVALCI+ELN+PSFYPS++S+WVNDSFERKDMER+LL KL ++L  +R+ +L++ HL  G  +VL +LEDA++DAP+A E+LGR+ A+F+
Sbjct: 1223 TLGPQGGLARGMSIRGQPLISNAELSSA----DSRRMVSGPNGYNSAST----AREEPGSR-IPDR--------SGR-------IAPNTQFAGPSNRPASQE---------GRSGNKLYSEDDLREKSISAIREYYSAKDEKEVALCIEELNAPSFYPSVVSLWVNDSFERKDMERELLTKLFVSLCNSRNNLLSKSHLTAGLATVLGSLEDALSDAPRAAEYLGRLLARFV 1421

[Close]

BLAST of CV090326 vs. Vitis vinifera peptide
Match: GSVIVT01018330001 (assembled CDS)

HSP 1 Score: 167.548 bits (423), Expect = 4.227e-42
Identity = 81/106 (76.42%), Postives = 93/106 (87.74%), Query Frame = 3

Query:  381 MSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFI 698
            MS+A IKEFYSAKDE EVALCIK+LNSP FYPSM+SIWV DSFERKD E D+LAKLL+NL+K+RD ML+Q  LI+GFE+VL+ LEDA+NDAPKA EFLGRIFA  I
Sbjct:    1 MSIAAIKEFYSAKDENEVALCIKDLNSPGFYPSMVSIWVTDSFERKDKEMDMLAKLLVNLTKSRDAMLSQVQLIKGFEAVLTALEDAVNDAPKAAEFLGRIFAMVI 106

[Close]

BLAST of CV090326 vs. TAIR peptide
Match: AT3G60240.4 (| Symbols: EIF4G, CUM2 | eukaryotic translation initiation factor 4G | chr3:22261842-22268295 FORWARD LENGTH=1727)

HSP 1 Score: 205.682 bits (522), Expect = 2.023e-53
Identity = 119/242 (49.17%), Postives = 157/242 (64.88%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAP--GPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSL-HEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSP------EDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFIS 701
            TLGPQGGL +GMSIR     SN   +DA   G GDSRR A GLNG+ S                   R A  + H +SS Q+R    V+R+      +SD S PE  + R    G +  V+SP      E++L ++S++ IKE+YSA+DE E+ +C+K++NSP+++P+MIS+WV DSFERKD ERDLLAKLL+NL K+ D  LN+  L++GFESVL TLEDA+NDAPKA EFLGRIF K ++
Sbjct: 1427 TLGPQGGLGQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGS------------------HRPASPVTHGRSSPQERGTAYVHREFASLSRASDLS-PEVSSARQVLQGPSATVNSPRENALSEEQLENLSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLVNLVKSADNALNEVQLVKGFESVLKTLEDAVNDAPKAAEFLGRIFGKSVT 1649

[Close]

BLAST of CV090326 vs. TAIR peptide
Match: AT3G60240.3 (| Symbols: EIF4G, CUM2 | eukaryotic translation initiation factor 4G | chr3:22261842-22268295 FORWARD LENGTH=1725)

HSP 1 Score: 205.682 bits (522), Expect = 2.023e-53
Identity = 119/242 (49.17%), Postives = 157/242 (64.88%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAP--GPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSL-HEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSP------EDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFIS 701
            TLGPQGGL +GMSIR     SN   +DA   G GDSRR A GLNG+ S                   R A  + H +SS Q+R    V+R+      +SD S PE  + R    G +  V+SP      E++L ++S++ IKE+YSA+DE E+ +C+K++NSP+++P+MIS+WV DSFERKD ERDLLAKLL+NL K+ D  LN+  L++GFESVL TLEDA+NDAPKA EFLGRIF K ++
Sbjct: 1425 TLGPQGGLGQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGS------------------HRPASPVTHGRSSPQERGTAYVHREFASLSRASDLS-PEVSSARQVLQGPSATVNSPRENALSEEQLENLSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLVNLVKSADNALNEVQLVKGFESVLKTLEDAVNDAPKAAEFLGRIFGKSVT 1647

[Close]

BLAST of CV090326 vs. TAIR peptide
Match: AT3G60240.2 (| Symbols: EIF4G, CUM2 | eukaryotic translation initiation factor 4G | chr3:22261842-22268295 FORWARD LENGTH=1723)

HSP 1 Score: 205.682 bits (522), Expect = 2.023e-53
Identity = 119/242 (49.17%), Postives = 157/242 (64.88%), Query Frame = 3

Query:    3 TLGPQGGLARGMSIRGQPSISNIPLADAP--GPGDSRRIAAGLNGYNSVSERTVYGREDLMPRYIPERFAGSL-HEQSSRQDRNMNPVNRDTRYADHSSDRSLPESPTIRGQGPGFAQNVSSP------EDRLRDMSMATIKEFYSAKDEKEVALCIKELNSPSFYPSMISIWVNDSFERKDMERDLLAKLLINLSKTRDGMLNQDHLIRGFESVLSTLEDAINDAPKAGEFLGRIFAKFIS 701
            TLGPQGGL +GMSIR     SN   +DA   G GDSRR A GLNG+ S                   R A  + H +SS Q+R    V+R+      +SD S PE  + R    G +  V+SP      E++L ++S++ IKE+YSA+DE E+ +C+K++NSP+++P+MIS+WV DSFERKD ERDLLAKLL+NL K+ D  LN+  L++GFESVL TLEDA+NDAPKA EFLGRIF K ++
Sbjct: 1423 TLGPQGGLGQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGS------------------HRPASPVTHGRSSPQERGTAYVHREFASLSRASDLS-PEVSSARQVLQGPSATVNSPRENALSEEQLENLSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLVNLVKSADNALNEVQLVKGFESVLKTLEDAVNDAPKAAEFLGRIFGKSVT 1645

The following BLAST results are available for this feature:

BLAST of CV090326 vs. DB:swissprot:display
BLAST of CV090326 vs. Populus trichocarpa peptide v2.0
BLAST of CV090326 vs. P. persica 1.0 peptide
BLAST of CV090326 vs. TrEMBL
BLAST of CV090326 vs. Vitis vinifera peptide
BLAST of CV090326 vs. TAIR peptide

BLAST of CV090326 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
IF4G1_ARATH	2.013e-52	49.17	Eukaryotic translation initiation factor 4G OS=Ara...	[more]
Y6969_DICDI	6.132e-9	28.17	von Willebrand factor A domain-containing protein ...	[more]

BLAST of CV090326 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
POPTR_0002s14110.1	4.917e-80	63.60		[more]
POPTR_0014s05150.1	1.868e-79	62.76		[more]

BLAST of CV090326 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ppa000085m	2.403e-81	65.69		[more]

BLAST of CV090326 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
B9MV40\|B9MV40_POPTR	2.406e-77	62.76	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PHK2\|A9PHK2_POPTR	2.406e-77	62.76	Putative uncharacterized protein OS=Populus tricho...	[more]
B9SKF9\|B9SKF9_RICCO	3.251e-74	64.58	Eukaryotic translation initiation factor 4g, putat...	[more]
C5XBH3\|C5XBH3_SORBI	7.792e-52	49.79	Putative uncharacterized protein Sb02g036110 OS=So...	[more]
Q0WVG9\|Q0WVG9_ARATH	2.961e-51	49.17	Initiation factor eIF-4 gamma like OS=Arabidopsis ...	[more]
A8MR97\|A8MR97_ARATH	2.961e-51	49.17	Uncharacterized protein At3g60240.2 OS=Arabidopsis...	[more]
D4N2J6\|D4N2J6_ORYSI	8.615e-51	50.86	Eukaryotic translation initiation factor 4g (Fragm...	[more]
D4N2J5\|D4N2J5_ORYSI	8.615e-51	50.86	Eukaryotic translation initiation factor 4g (Fragm...	[more]
D7LRN0\|D7LRN0_ARALY	1.470e-50	49.17	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q69S49\|Q69S49_ORYSJ	2.507e-50	50.43	Putative eukaryotic translation initiation factor ...	[more]

BLAST of CV090326 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1


Match Name	E-value	Identity	Description
GSVIVT01018330001	4.227e-42	76.42	assembled CDS	[more]

BLAST of CV090326 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT3G60240.4	2.023e-53	49.17	\| Symbols: EIF4G, CUM2 \| eukaryotic translation in...	[more]
AT3G60240.3	2.023e-53	49.17	\| Symbols: EIF4G, CUM2 \| eukaryotic translation in...	[more]
AT3G60240.2	2.023e-53	49.17	\| Symbols: EIF4G, CUM2 \| eukaryotic translation in...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003891	Initiation factor eIF-4 gamma, MA3	PFAM	PF02847	MA3	coord: 128..232 score: 9.2
IPR003891	Initiation factor eIF-4 gamma, MA3	SMART	SM00544	no description	coord: 124..233 score: 0.
IPR003891	Initiation factor eIF-4 gamma, MA3	PROFILE	PS51366	MI	coord: 123..233 score: 18
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 116..233 score: 5.8
None	No IPR available	PANTHER	PTHR23253	EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA	coord: 1..232 score: 1.2
None	No IPR available	PANTHER	PTHR23253:SF7	EUKARYOTIC TRANSLATION INITIATION FACTOR 4G	coord: 1..232 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV090326 ID=CV090326; Name=CV090326; organism=Vaccinium corymbosum; type=EST; length=703bp
TTACACTTGGACCCCAAGGAGGTCTTGCAAGGGGAATGTCCATCAGGGGA
CAGCCATCAATTTCGAACATTCCTTTAGCTGATGCACCAGGTCCTGGTGA
TTCCAGAAGAATCGCAGCTGGTCTGAATGGTTACAATTCTGTATCAGAAC
GGACGGTTTATGGAAGAGAGGATCTCATGCCTAGATACATCCCGGAAAGG
TTTGCAGGTTCTCTTCATGAACAGTCAAGCAGACAAGATCGCAATATGAA
CCCTGTTAACAGAGACACAAGGTATGCAGATCACAGTTCCGATAGATCTT
TGCCTGAGTCACCAACCATTCGTGGTCAGGGACCTGGTTTTGCGCAAAAT
GTTTCATCGCCAGAAGACCGCTTGCGAGACATGTCCATGGCAACAATTAA
AGAATTCTACAGCGCCAAAGATGAGAAGGAAGTTGCACTGTGCATAAAGG
AATTGAATTCCCCGAGCTTTTATCCCTCGATGATATCTATCTGGGTCAAC
GATTCTTTTGAGAGGAAAGATATGGAGAGGGATCTTTTGGCCAAGTTACT
CATCAACCTTTCAAAAACTCGGGATGGCATGTTAAACCAAGATCATCTCA
TCAGAGGGTTTGAATCTGTTCTTTCTACATTGGAAGATGCAATAAATGAT
GCCCCAAAAGCAGGAGAATTCCTCGGCCGTATCTTTGCAAAATTCATTTC
AGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005488	binding

Vocabulary: **KEGG ORTHOLOGY**
Term	Definition
K03260	eIF-4F, EIF4G; translation initiation factor eIF-4F

Vocabulary: **KEGG PATHWAY**
Term	Definition
ko03010	Ribosome
ko05410	Hypertrophic cardiomyopathy (HCM)

Vocabulary: **INTERPRO**
Term	Definition
IPR003891	Initiation_fac_eIF4g_MI
IPR016024	ARM-type_fold
noIPR	unintegrated