CV191123 | GDV

EST Overview


Name	CV191123
Unique Name	CV191123
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	471

Libraries


Library Name	Type
LIBEST_016374 Vco01	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18
KEGG analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_ARATH (NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana GN=ECR1 PE=1 SV=2)

HSP 1 Score: 176.407 bits (446), Expect = 5.474e-44
Identity = 87/143 (60.84%), Postives = 105/143 (73.43%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                CALETLKIVS CSKTL NYLTYNG EGL+T+VT+F +D +CL+CGPG+LIEL+TSVTL KFI +LE+HPKLLLS+AS+      LYMQAPPVLEE  R  L  PL++LM ++ KD +HV
Sbjct:  275 SLTQGVVKNIIPAIASTNAIISAACALETLKIVSACSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKLLLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHV 417

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_DROME (NEDD8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster GN=CG13343 PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 5.525e-12
Identity = 47/139 (33.81%), Postives = 67/139 (48.20%), Query Frame = 2

Query:    5 LTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICG--PGVL-IELETSVTLRKFIGLLEEHPKLLLSRASIT-----HRGKNLYMQAPPVLEEMTRSNLDLPLFEL 397
            L QGVVK                CALE  K+ + C  +++NYL +N ++G++T   E  K ++CL C   P  L IE   + TL   I LL + P+  L   ++T      + + LYM     +EE TR NL   L EL
Sbjct:  279 LVQGVVKHIIPAVASTNAAIAAACALEVFKLATSCYDSMANYLNFNDLDGIYTYTYEAEKSENCLACSNTPQPLPIEDPNTTTLEDVIKLLCDSPRFQLKSPALTTVMKDGKRRTLYMSGVKSIEEATRKNLTQSLGEL 417

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_DICDI (NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum GN=uba3 PE=1 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.099e-11
Identity = 42/132 (31.82%), Postives = 62/132 (46.97%), Query Frame = 2

Query:    5 LTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPG-VLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFEL 397
            LTQGV K                C  E  K  +  S  L+NY+ YNG+ G++T   E+   + C +CG   V  E++ S TL  F+  +    +    + S+   G+NLYMQ   +L + T  NL+  L EL
Sbjct:  284 LTQGVAKNIIPAIASTNAIIAAACCNEVFKFCTDSSGYLNNYMMYNGLNGVYTFTFEYEIKEGCAVCGTNLVTFEIDKSNTLSTFLEKITTDSRFQFKKPSLRSNGRNLYMQG--LLHQSTVPNLEKTLSEL 413

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_DANRE (NEDD8-activating enzyme E1 catalytic subunit OS=Danio rerio GN=uba3 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.321e-10
Identity = 44/136 (32.35%), Postives = 63/136 (46.32%), Query Frame = 2

Query:    5 LTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVL-IELETSVTLRKFIGLLEEHPKLLLSRASIT----HRGKNLYMQAPPVLEEMTRSNLDLPLFEL 397
            LTQGVVK                CA E  KI +     L+NYL +N V+GL+T   E  + ++C  C      ++   S  L++ +  L E+  L +   +IT     + K LY+Q    +EE TR NL   L EL
Sbjct:  300 LTQGVVKRIIPAVASTNAVIAAACATEVFKIATSAYVPLNNYLVFNDVDGLYTYTFEAERKENCSACSQVPQDMQFTPSAKLQEVLDYLTENASLQMKSPAITTTLDGKNKTLYLQTVASIEERTRPNLSKTLKEL 435

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_PONAB (NEDD8-activating enzyme E1 catalytic subunit OS=Pongo abelii GN=UBA3 PE=2 SV=2)

HSP 1 Score: 63.1586 bits (152), Expect = 6.754e-10
Identity = 46/136 (33.82%), Postives = 63/136 (46.32%), Query Frame = 2

Query:    5 LTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGP-GVLIELETSVTLRKFIGLLEEHPKLLLSRASITH--RGKN--LYMQAPPVLEEMTRSNLDLPLFEL 397
            LTQGVVK                CA E  KI +     L+NYL +N V+GL+T   E  + ++C  C      I+   S  L++ +  L     L +   +IT    GKN  LY+Q+   +EE TR NL   L EL
Sbjct:  301 LTQGVVKRIIPAVASTNAVVAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDFLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_RAT (NEDD8-activating enzyme E1 catalytic subunit OS=Rattus norvegicus GN=Uba3 PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 8.820e-10
Identity = 46/136 (33.82%), Postives = 63/136 (46.32%), Query Frame = 2

Query:    5 LTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGP-GVLIELETSVTLRKFIGLLEEHPKLLLSRASITH--RGKN--LYMQAPPVLEEMTRSNLDLPLFEL 397
            LTQGVVK                CA E  KI +     L+NYL +N V+GL+T   E  + ++C  C      I+   S  L++ +  L     L +   +IT    GKN  LY+Q+   +EE TR NL   L EL
Sbjct:  301 LTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_MOUSE (NEDD8-activating enzyme E1 catalytic subunit OS=Mus musculus GN=Uba3 PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 8.820e-10
Identity = 46/136 (33.82%), Postives = 63/136 (46.32%), Query Frame = 2

Query:    5 LTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGP-GVLIELETSVTLRKFIGLLEEHPKLLLSRASITH--RGKN--LYMQAPPVLEEMTRSNLDLPLFEL 397
            LTQGVVK                CA E  KI +     L+NYL +N V+GL+T   E  + ++C  C      I+   S  L++ +  L     L +   +IT    GKN  LY+Q+   +EE TR NL   L EL
Sbjct:  301 LTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQIKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_HUMAN (NEDD8-activating enzyme E1 catalytic subunit OS=Homo sapiens GN=UBA3 PE=1 SV=2)

HSP 1 Score: 62.7734 bits (151), Expect = 8.820e-10
Identity = 46/136 (33.82%), Postives = 63/136 (46.32%), Query Frame = 2

Query:    5 LTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGP-GVLIELETSVTLRKFIGLLEEHPKLLLSRASITH--RGKN--LYMQAPPVLEEMTRSNLDLPLFEL 397
            LTQGVVK                CA E  KI +     L+NYL +N V+GL+T   E  + ++C  C      I+   S  L++ +  L     L +   +IT    GKN  LY+Q+   +EE TR NL   L EL
Sbjct:  301 LTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_CAEEL (NEDD8-activating enzyme E1 catalytic subunit OS=Caenorhabditis elegans GN=uba-3 PE=2 SV=2)

HSP 1 Score: 60.077 bits (144), Expect = 5.717e-9
Identity = 40/135 (29.63%), Postives = 62/135 (45.93%), Query Frame = 2

Query:    5 LTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVL-IELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAP--PVLEEMTRSNLDLPLFELM 400
            LT GV+K                CALE LK+ +  +K + NYL +  + G +T V   +KD +CL C  G L  E+  S TL   I  L E  +  L   ++    + LY  +   P  E+ ++ NL   + +L+
Sbjct:  274 LTSGVLKRIIPAVASTNAVIAASCALEALKLATNIAKPIDNYLNFTQIHGAYTSVVSMMKDDNCLTCSGGRLPFEVSPSSTLESLIIRLSE--RFHLKHPTLATSTRKLYCISSFMPQFEQESKENLHTSMKDLV 406

[Close]

BLAST of CV191123 vs. DB:swissprot:display
Match: UBA3_SCHPO (NEDD8-activating enzyme E1 catalytic subunit OS=Schizosaccharomyces pombe GN=uba3 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.840e-8
Identity = 40/134 (29.85%), Postives = 59/134 (44.03%), Query Frame = 2

Query:   11 QGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIEL-----ETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFEL 397
            QG+VK                C  E LKI++  +  L NY+ Y G +G +T      K  DC +C  GVL E+      ++VTL+  +    +   L     S T  G  LY+ +PP L+  T  NL  P+  +
Sbjct:  289 QGIVKRIIPAVASTNAIIAASCCNEALKILTESNPFLDNYMMYVGEDGAYTYTFNLEKRSDCPVC--GVLSEVYDISASSTVTLKDILNHYSKSYNLQNPSVS-TAAGTPLYLASPPALQVATSKNLSQPILSI 419

[Close]

BLAST of CV191123 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0008s20850.1 ()

HSP 1 Score: 219.935 bits (559), Expect = 5.599e-58
Identity = 108/143 (75.52%), Postives = 118/143 (82.52%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                C LETLK+ SGCSKTLSNYLTYNGVEGLH KVTEFVKDKDCL+CGPGVL+EL+TSVTL+KFI +LEEHPKLLLS+AS+ HR  NLYMQAPPVLEEMTRSNL+LPLFELM K PKDIVHV
Sbjct:  271 SLTQGVVKNIIPAIASTNAIISAACTLETLKLASGCSKTLSNYLTYNGVEGLHIKVTEFVKDKDCLVCGPGVLLELDTSVTLQKFIDMLEEHPKLLLSKASVRHRATNLYMQAPPVLEEMTRSNLNLPLFELMGKFPKDIVHV 413

[Close]

BLAST of CV191123 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0010s03520.1 ()

HSP 1 Score: 210.305 bits (534), Expect = 4.436e-55
Identity = 105/143 (73.43%), Postives = 116/143 (81.12%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                C LETLK+ SGCSKTLSNYLTYNGVEGLH KVTEFV+DKDCL+CGPGVLIEL+TSVTL+KFI +LEEHPKLLLS+AS+ H+  NLYMQAPPVLEEMTRSNL LPLFELM K+ KDIV V
Sbjct:  271 SLTQGVVKNIIPAIASTNAIISAACTLETLKLASGCSKTLSNYLTYNGVEGLHIKVTEFVRDKDCLVCGPGVLIELDTSVTLQKFIDMLEEHPKLLLSKASVRHQTMNLYMQAPPVLEEMTRSNLGLPLFELMGKVSKDIVLV 413

[Close]

BLAST of CV191123 vs. P. persica 1.0 peptide
Match: ppa005634m ()

HSP 1 Score: 219.164 bits (557), Expect = 6.969e-58
Identity = 106/142 (74.65%), Postives = 119/142 (83.80%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVH 427
            +LTQGVVK                C LETLKI SGCSKTLSNYLTYNG EGLHT+VTEFVKDKDCL+CGPGVLI+L+TS+TL KFI LL+EHPKLLLS+ASITHRGK+LYMQAPPVLEEMTRSNL +PLF+L+ KIPKD+VH
Sbjct:  272 SLTQGVVKNIIPAIASTNAIISAACVLETLKIASGCSKTLSNYLTYNGAEGLHTEVTEFVKDKDCLVCGPGVLIQLDTSITLEKFIDLLKEHPKLLLSKASITHRGKSLYMQAPPVLEEMTRSNLSIPLFDLIGKIPKDVVH 413

[Close]

BLAST of CV191123 vs. TrEMBL
Match: B9RTW5|B9RTW5_RICCO (Ubiquitin-activating enzyme E1c, putative OS=Ricinus communis GN=RCOM_0913520 PE=4 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 3.078e-58
Identity = 114/143 (79.72%), Postives = 119/143 (83.22%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                CALETLKI SGCSKTLSNYLTYNGVEGLH KVTEFVKDKDCL CGPGVLIEL+TSV L+KFI LLEEHPKL LSRAS+THRGKNLYMQAPPVLEEMTRSNL LPLFELM K+PKDIVHV
Sbjct:  271 SLTQGVVKNIIPAIASTNAIISAACALETLKIASGCSKTLSNYLTYNGVEGLHIKVTEFVKDKDCLACGPGVLIELDTSVALQKFIDLLEEHPKLFLSRASVTHRGKNLYMQAPPVLEEMTRSNLSLPLFELMGKVPKDIVHV 413

[Close]

BLAST of CV191123 vs. TrEMBL
Match: B9HMN4|B9HMN4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_803920 PE=4 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 6.418e-56
Identity = 108/143 (75.52%), Postives = 118/143 (82.52%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                C LETLK+ SGCSKTLSNYLTYNGVEGLH KVTEFVKDKDCL+CGPGVL+EL+TSVTL+KFI +LEEHPKLLLS+AS+ HR  NLYMQAPPVLEEMTRSNL+LPLFELM K PKDIVHV
Sbjct:  271 SLTQGVVKNIIPAIASTNAIISAACTLETLKLASGCSKTLSNYLTYNGVEGLHIKVTEFVKDKDCLVCGPGVLLELDTSVTLQKFIDMLEEHPKLLLSKASVRHRATNLYMQAPPVLEEMTRSNLNLPLFELMGKFPKDIVHV 413

[Close]

BLAST of CV191123 vs. TrEMBL
Match: D7T6S7|D7T6S7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_20.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00017905001 PE=4 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 1.581e-54
Identity = 108/143 (75.52%), Postives = 119/143 (83.22%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                CALETLKIVSGCSKTLSNYLTYNG EGLHTKVTEFVKD DCLICGPGVLIEL+TSVTL+KF+  L+EHPKLLLS+AS+ HR KNLY+QAPPVLEE+TRSNL+LPLF+LM KI KDIVHV
Sbjct:  271 SLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDMDCLICGPGVLIELDTSVTLQKFMDQLQEHPKLLLSKASVKHREKNLYIQAPPVLEELTRSNLNLPLFDLMGKIRKDIVHV 413

[Close]

BLAST of CV191123 vs. TrEMBL
Match: B9HY52|B9HY52_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_768711 PE=4 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 5.085e-53
Identity = 105/143 (73.43%), Postives = 116/143 (81.12%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                C LETLK+ SGCSKTLSNYLTYNGVEGLH KVTEFV+DKDCL+CGPGVLIEL+TSVTL+KFI +LEEHPKLLLS+AS+ H+  NLYMQAPPVLEEMTRSNL LPLFELM K+ KDIV V
Sbjct:  271 SLTQGVVKNIIPAIASTNAIISAACTLETLKLASGCSKTLSNYLTYNGVEGLHIKVTEFVRDKDCLVCGPGVLIELDTSVTLQKFIDMLEEHPKLLLSKASVRHQTMNLYMQAPPVLEEMTRSNLGLPLFELMGKVSKDIVLV 413

[Close]

BLAST of CV191123 vs. TrEMBL
Match: B8LQD0|B8LQD0_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 5.446e-47
Identity = 92/143 (64.34%), Postives = 109/143 (76.22%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            + TQGVVK                CALE LKIV+G SK LSNY TYN + GLHT V+EFVK+ DCL+CGPGVLIEL+T+VTL+KF+ LL EHP L +SR S+ ++G NLYMQAPPVLEEMTRSNL +PLFELM K+PKD+VHV
Sbjct:  269 SFTQGVVKNIVPAIAATNAIISSTCALEALKIVTGFSKILSNYSTYNSIAGLHTSVSEFVKENDCLVCGPGVLIELDTTVTLQKFMDLLAEHPMLKMSRTSVMYQGNNLYMQAPPVLEEMTRSNLQIPLFELMGKVPKDVVHV 411

[Close]

BLAST of CV191123 vs. TrEMBL
Match: B4FVB9|B4FVB9_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 4.610e-46
Identity = 87/142 (61.27%), Postives = 108/142 (76.06%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVH 427
            +LTQGVVK                CALE LK++SGCSK++SNYLTYNG+EG H KVTEFV+DKDCL+CGPG L+EL+TS TL  FI +LEEHPKL +S+AS+TH G NLYMQ+P VLE+MTR NL +P+FEL+ ++P   VH
Sbjct:  277 SLTQGVVKNIIPAIASTNAIISAACALEALKLISGCSKSVSNYLTYNGLEGTHIKVTEFVRDKDCLVCGPGTLVELDTSSTLSDFIKMLEEHPKLRMSKASVTHEGNNLYMQSPEVLEQMTRPNLSIPMFELLKEVPYTTVH 418

[Close]

BLAST of CV191123 vs. TrEMBL
Match: B6TJH0|B6TJH0_MAIZE (NEDD8-activating enzyme E1 catalytic subunit OS=Zea mays PE=2 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 6.021e-46
Identity = 87/142 (61.27%), Postives = 108/142 (76.06%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVH 427
            +LTQGVVK                CALE LK++SGCSK++SNYLTYNG+EG H KVTEFV+DKDCL+CGPG L+EL+TS TL  FI +LEEHPKL +S+AS+TH G NLYMQ+P VLE+MTR NL +P+FEL+ ++P   VH
Sbjct:  277 SLTQGVVKNIIPAIASTNAIISAACALEALKLISGCSKSVSNYLTYNGLEGTHIKVTEFVRDKDCLVCGPGTLVELDTSSTLSDFIKMLEEHPKLRMSKASVTHEGNNLYMQSPEVLEQMTRPNLSVPMFELLKEVPYTTVH 418

[Close]

BLAST of CV191123 vs. TrEMBL
Match: C5XH65|C5XH65_SORBI (Putative uncharacterized protein Sb03g010860 OS=Sorghum bicolor GN=Sb03g010860 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.937e-44
Identity = 84/142 (59.15%), Postives = 106/142 (74.65%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVH 427
            +LTQGVVK                CALE  K++SGCSK++SNYLTYNG+EG H KVTEF++DKDCL+CGPG LIEL+T+ TL  FI +LEEHP L +S+AS+TH G NLYMQ+P VLE+MTR NL +P+FEL+ ++P   VH
Sbjct:  277 SLTQGVVKNIIPAIASTNAIISAACALEAFKLISGCSKSVSNYLTYNGLEGTHIKVTEFIRDKDCLVCGPGTLIELDTTSTLSDFIKMLEEHPTLRMSKASVTHEGNNLYMQSPEVLEQMTRPNLSVPMFELLKEVPYTTVH 418

[Close]

BLAST of CV191123 vs. TrEMBL
Match: D7LYM6|D7LYM6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_909950 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 3.304e-44
Identity = 89/143 (62.24%), Postives = 106/143 (74.13%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                CALETLKIVS CSKTL NYLTYNG EGL+T+VT+F +D DCL+CGPG+LIEL+TSVTL KFI +LE+HPKLLLS+AS+ H    LYMQAPPVLEE  R  L  PL++LM ++ KD +HV
Sbjct:  275 SLTQGVVKNIIPAIASTNAIISAACALETLKIVSACSKTLVNYLTYNGGEGLYTEVTKFERDTDCLVCGPGILIELDTSVTLSKFIEMLEDHPKLLLSKASVKHGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHV 417

[Close]

BLAST of CV191123 vs. TrEMBL
Match: Q5NBF4|Q5NBF4_ORYSJ (Os01g0271500 protein OS=Oryza sativa subsp. japonica GN=P0693B08.15 PE=4 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 4.315e-44
Identity = 86/143 (60.14%), Postives = 105/143 (73.43%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            + TQGVVK                CALE LK++SGCSKT+SNYLTYNG++G H  V+EF ++KDCL+CGPG LIEL TS TL +FI +LEEHPKLL+SRAS+TH G NLYMQAP VLE+MTR NL +P+FEL+    +  VHV
Sbjct:  275 SFTQGVVKNIIPAIASTNAIVSAACALEALKLISGCSKTVSNYLTYNGLDGTHINVSEFAREKDCLVCGPGTLIELGTSTTLSEFIKMLEEHPKLLMSRASVTHEGDNLYMQAPEVLEQMTRPNLGVPMFELLKGAARTTVHV 417

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BLAST of CV191123 vs. Vitis vinifera peptide
Match: GSVIVT01017905001 (assembled CDS)

HSP 1 Score: 215.312 bits (547), Expect = 8.670e-57
Identity = 108/143 (75.52%), Postives = 119/143 (83.22%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                CALETLKIVSGCSKTLSNYLTYNG EGLHTKVTEFVKD DCLICGPGVLIEL+TSVTL+KF+  L+EHPKLLLS+AS+ HR KNLY+QAPPVLEE+TRSNL+LPLF+LM KI KDIVHV
Sbjct:  271 SLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDMDCLICGPGVLIELDTSVTLQKFMDQLQEHPKLLLSKASVKHREKNLYIQAPPVLEELTRSNLNLPLFDLMGKIRKDIVHV 413

[Close]

BLAST of CV191123 vs. TAIR peptide
Match: AT5G19180.1 (| Symbols: ECR1 | E1 C-terminal related 1 | chr5:6453375-6455750 FORWARD LENGTH=454)

HSP 1 Score: 176.407 bits (446), Expect = 6.286e-45
Identity = 87/143 (60.84%), Postives = 105/143 (73.43%), Query Frame = 2

Query:    2 ALTQGVVKXXXXXXXXXXXXXXXXCALETLKIVSGCSKTLSNYLTYNGVEGLHTKVTEFVKDKDCLICGPGVLIELETSVTLRKFIGLLEEHPKLLLSRASITHRGKNLYMQAPPVLEEMTRSNLDLPLFELMDKIPKDIVHV 430
            +LTQGVVK                CALETLKIVS CSKTL NYLTYNG EGL+T+VT+F +D +CL+CGPG+LIEL+TSVTL KFI +LE+HPKLLLS+AS+      LYMQAPPVLEE  R  L  PL++LM ++ KD +HV
Sbjct:  275 SLTQGVVKNIIPAIASTNAIISAACALETLKIVSACSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKLLLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHV 417

The following BLAST results are available for this feature:

BLAST of CV191123 vs. DB:swissprot:display
BLAST of CV191123 vs. Populus trichocarpa peptide v2.0
BLAST of CV191123 vs. P. persica 1.0 peptide
BLAST of CV191123 vs. TrEMBL
BLAST of CV191123 vs. Vitis vinifera peptide
BLAST of CV191123 vs. TAIR peptide

BLAST of CV191123 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
UBA3_ARATH	5.474e-44	60.84	NEDD8-activating enzyme E1 catalytic subunit OS=Ar...	[more]
UBA3_DROME	5.525e-12	33.81	NEDD8-activating enzyme E1 catalytic subunit OS=Dr...	[more]
UBA3_DICDI	2.099e-11	31.82	NEDD8-activating enzyme E1 catalytic subunit OS=Di...	[more]
UBA3_DANRE	2.321e-10	32.35	NEDD8-activating enzyme E1 catalytic subunit OS=Da...	[more]
UBA3_PONAB	6.754e-10	33.82	NEDD8-activating enzyme E1 catalytic subunit OS=Po...	[more]
UBA3_RAT	8.820e-10	33.82	NEDD8-activating enzyme E1 catalytic subunit OS=Ra...	[more]
UBA3_MOUSE	8.820e-10	33.82	NEDD8-activating enzyme E1 catalytic subunit OS=Mu...	[more]
UBA3_HUMAN	8.820e-10	33.82	NEDD8-activating enzyme E1 catalytic subunit OS=Ho...	[more]
UBA3_CAEEL	5.717e-9	29.63	NEDD8-activating enzyme E1 catalytic subunit OS=Ca...	[more]
UBA3_SCHPO	4.840e-8	29.85	NEDD8-activating enzyme E1 catalytic subunit OS=Sc...	[more]

BLAST of CV191123 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
POPTR_0008s20850.1	5.599e-58	75.52		[more]
POPTR_0010s03520.1	4.436e-55	73.43		[more]

BLAST of CV191123 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ppa005634m	6.969e-58	74.65		[more]

BLAST of CV191123 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
B9RTW5\|B9RTW5_RICCO	3.078e-58	79.72	Ubiquitin-activating enzyme E1c, putative OS=Ricin...	[more]
B9HMN4\|B9HMN4_POPTR	6.418e-56	75.52	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7T6S7\|D7T6S7_VITVI	1.581e-54	75.52	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HY52\|B9HY52_POPTR	5.085e-53	73.43	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B8LQD0\|B8LQD0_PICSI	5.446e-47	64.34	Putative uncharacterized protein OS=Picea sitchens...	[more]
B4FVB9\|B4FVB9_MAIZE	4.610e-46	61.27	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6TJH0\|B6TJH0_MAIZE	6.021e-46	61.27	NEDD8-activating enzyme E1 catalytic subunit OS=Ze...	[more]
C5XH65\|C5XH65_SORBI	1.937e-44	59.15	Putative uncharacterized protein Sb03g010860 OS=So...	[more]
D7LYM6\|D7LYM6_ARALY	3.304e-44	62.24	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q5NBF4\|Q5NBF4_ORYSJ	4.315e-44	60.14	Os01g0271500 protein OS=Oryza sativa subsp. japoni...	[more]

BLAST of CV191123 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1


Match Name	E-value	Identity	Description
GSVIVT01017905001	8.670e-57	75.52	assembled CDS	[more]

BLAST of CV191123 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G19180.1	6.286e-45	60.84	\| Symbols: ECR1 \| E1 C-terminal related 1 \| chr5:6...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000127	Ubiquitin-activating enzyme repeat	PFAM	PF02134	UBACT	coord: 3..36 score: 5.3
IPR009036	Molybdenum cofactor biosynthesis, MoeB	SUPERFAMILY	69572	Activating enzymes of the ubiquitin-like proteins	coord: 2..154 score: 5.5
IPR014929	E2 binding	PFAM	PF08825	E2_bind	coord: 74..154 score: 3.2
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 2..52 score: 9
None	No IPR available	GENE3D	3.10.20.260	no description	coord: 63..116 score: 1.1
None	No IPR available	PANTHER	PTHR10953	UBIQUITIN-ACTIVATING ENZYME E1	coord: 2..33 score: 1
None	No IPR available	PANTHER	PTHR10953:SF6	UBIQUITIN-ACTIVATING ENZYME E1C	coord: 2..33 score: 1
None	No IPR available	SEG	seg	seg	coord: 9..24 score: NAcoord: 144..156 score

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV191123 ID=CV191123; Name=CV191123; organism=Vaccinium corymbosum; type=EST; length=471bp
GGCTCTGACCCAGGGTGTTGTAAAAAATATTATACCTGCCATAGCTTCCA
CCAATGCAATTATATCAGCTGCGTGCGCCCTTGAAACCTTGAAGATTGTC
TCTGGATGCAGCAAAACATTGTCCAATTATTTGACATATAATGGTGTTGA
AGGTCTCCACACTAAAGTGACTGAGTTTGTGAAAGACAAAGACTGTCTTA
TATGTGGTCCTGGTGTTCTCATTGAGCTAGAAACTTCAGTTACTTTACGA
AAGTTTATTGGTTTACTTGAAGAGCACCCTAAGCTACTCCTATCCAGAGC
AAGTATTACACATCGTGGGAAGAATCTGTACATGCAGGCACCTCCTGTTC
TAGAAGAGATGACAAGATCCAACCTTGATCTTCCTCTTTTTGAGCTGATG
GATAAGATTCCAAAAGACATTGTCCATGTGACAGGTACCACGAGCAAGAG
TGACAAGAAGACATCATCGTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0008641	ubiquitin-like modifier activating enzyme activity
GO:0016881	acid-amino acid ligase activity
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006464	cellular protein modification process
GO:0045116	protein neddylation
GO:0008152	metabolic process

Vocabulary: **KEGG ORTHOLOGY**
Term	Definition
K10686	UBE1C, UBA3; ubiquitin-activating enzyme E1 C [EC:6.3.2.19]

Vocabulary: **INTERPRO**
Term	Definition
IPR000127	Ubiquitin-activating enzyme repeat
IPR009036	Molybdenum cofactor biosynthesis, MoeB
IPR014929	E2-binding
IPR016040	NAD(P)-bd_dom
noIPR	unintegrated