CV091064 | GDV

EST Overview


Name	CV091064
Unique Name	CV091064
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	712

Libraries


Library Name	Type
LIBEST_014531 cDNA non acclimated Bluecrop library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18
KEGG analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC_ARATH (3-isopropylmalate dehydratase OS=Arabidopsis thaliana GN=IIL1 PE=2 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 1.899e-66
Identity = 121/165 (73.33%), Postives = 136/165 (82.42%), Query Frame = 3

Query:  219 QKDVGLSAFSSQPSFSI-RNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            + D+G+S+F      SI R  KK +SRKI  VMAPQ+ +R P T+GSVKT MTMTEKILARASEK  + PG+N+WVNVDVLMTHDVCGPG FGIFK+EFG+ AKVWD EK+V+IPDHYIFT D RANRNVDI RE C EQNIKYFYDI DLGNFKANPDYKGVCH
Sbjct:   17 KSDLGISSFPKSSQISIHRCQKKSISRKIVSVMAPQK-DRSPGTTGSVKTGMTMTEKILARASEKSLVVPGDNIWVNVDVLMTHDVCGPGAFGIFKREFGEKAKVWDPEKIVVIPDHYIFTADKRANRNVDIMREHCREQNIKYFYDITDLGNFKANPDYKGVCH 180

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC_METJA (Isopropylmalate/citramalate isomerase large subunit OS=Methanocaldococcus jannaschii GN=leuC PE=1 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.129e-18
Identity = 52/114 (45.61%), Postives = 70/114 (61.40%), Query Frame = 3

Query:  369 MTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            MT+ EKILA+AS K+++SPG+ V  N+DV M HD+ GP T     KE+G   KVW+ EK+VI+ DH +     +A  N  + R+F  EQ IKYFYDI++           GVCH
Sbjct:    3 MTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTL-KEYGIE-KVWNPEKIVILFDHQVPADSIKAAENHILMRKFVKEQGIKYFYDIRE-----------GVCH 103

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC1_METTH (3-isopropylmalate dehydratase large subunit 1 OS=Methanobacterium thermoautotrophicum GN=leuC1 PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 6.848e-16
Identity = 48/115 (41.74%), Postives = 70/115 (60.87%), Query Frame = 3

Query:  366 AMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            +MT++EKILARAS K ++  GE V  ++DV MTHD+ GP +   F +  G++ +VWD EK+V+I DH +      A +N  I R+F  EQ I+ FYD+++           GVCH
Sbjct:    2 SMTVSEKILARASGKDRVEAGEIVMADIDVAMTHDLTGPLSVESF-RAIGED-RVWDPEKIVVIFDHQVPADSIEAAQNHMIMRDFVEEQGIRNFYDVRE-----------GVCH 103

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC_SYNAS (3-isopropylmalate dehydratase large subunit OS=Syntrophus aciditrophicus (strain SB) GN=leuC PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.181e-13
Identity = 41/112 (36.61%), Postives = 65/112 (58.04%), Query Frame = 3

Query:  369 MTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGV 704
            MT+TEKIL R ++  ++ PG  +   VD+ + +DV  P     F+K  G+  KV+DR+KVV+IPDH+    D  +     I R+F  EQ I ++Y++ ++G   A    KG+
Sbjct:    3 MTITEKILCRHTDLDEVRPGMLINAKVDIALGNDVTAPLAIDEFRKAGGK--KVFDRDKVVLIPDHFTPNKDINSAAQCKIMRDFAREQEITHYYEVGEVGVEHALLPEKGI 112

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC1_METKA (3-isopropylmalate dehydratase large subunit 1 OS=Methanopyrus kandleri GN=leuC1 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.062e-12
Identity = 45/113 (39.82%), Postives = 61/113 (53.98%), Query Frame = 3

Query:  372 TMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            TM EKIL+RAS  +    G+ V  N+DV M HD+ GP T    + E G   +VWD  K+V++ DH +      A  N  I REF  EQ I++FYD+++           GVCH
Sbjct:    4 TMAEKILSRASG-EDAEAGDIVVANIDVAMVHDITGPITVQRLE-EMGVE-RVWDPSKIVVLFDHQVPADSVEAAENHKIMREFVEEQGIEHFYDVRE-----------GVCH 102

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC_DESAA (3-isopropylmalate dehydratase large subunit OS=Desulfatibacillum alkenivorans (strain AK-01) GN=leuC PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.999e-12
Identity = 37/112 (33.04%), Postives = 66/112 (58.93%), Query Frame = 3

Query:  369 MTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGV 704
            MT+ EKILA  + +++ SPG+ +   VD+ + +D+  P    +F++     AKV+D+++VV++PDH+  T D  +   V + R+F  EQ + ++Y+  D G   A    KG+
Sbjct:    3 MTIAEKILAAHAGQEEASPGDLINAKVDIALGNDITAPIAIKLFRQSGA--AKVFDKDRVVLVPDHFTPTKDINSAMQVKMVRDFAREQELSHWYEGGDSGVEHALLPEKGI 112

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC1_ARCFU (3-isopropylmalate dehydratase large subunit 1 OS=Archaeoglobus fulgidus GN=leuC1 PE=3 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 1.745e-11
Identity = 38/99 (38.38%), Postives = 56/99 (56.57%), Query Frame = 3

Query:  366 AMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIK 662
            A T+ EKI ++AS K+ +  GE V  N+D+ M HD+  P     F++  G +AKVWD+ KV++  DH +      A  N  + R+F  EQ I   YD+K
Sbjct:    2 AQTLVEKIFSKASGKE-VKAGEFVMANIDLAMIHDITAPLAIKAFREILGSDAKVWDKSKVIMAFDHQVPADSVHAAENHKMLRKFAEEQGI-LNYDVK 98

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC_THEYD (3-isopropylmalate dehydratase large subunit OS=Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) GN=leuC PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.662e-11
Identity = 33/101 (32.67%), Postives = 62/101 (61.39%), Query Frame = 3

Query:  369 MTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLG 671
            MT+ EKILA  + K++++PGE +   VD+++ +D+  P     F+K   ++  V+DRE++ ++PDH+    D +A     + REF  +  IK++++I  +G
Sbjct:    3 MTIAEKILAAHAGKKEVTPGEIINAKVDIVLANDITAPIAIKEFEKLGVKD--VFDRERIALVPDHFTPQKDIKAAEQCKMLREFAQKYKIKHYFEIGRVG 101

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC_CALS8 (3-isopropylmalate dehydratase large subunit OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=leuC PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.662e-11
Identity = 33/101 (32.67%), Postives = 65/101 (64.36%), Query Frame = 3

Query:  369 MTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLG 671
            MTM++KILA  + K+ + PG+ ++ NVD+++ +DV  P     F+K  G + KV+D++K+ I+PDH+    D ++ +   + REF  + +I  ++++ ++G
Sbjct:    5 MTMSQKILAYHAGKEYVEPGDLIFANVDLVLGNDVTTPVAIKEFEK-IGVD-KVFDKDKITIVPDHFTPNKDIKSAQQCKMVREFAKKYDITNYFEVGEMG 103

[Close]

BLAST of CV091064 vs. DB:swissprot:display
Match: LEUC_HELMI (3-isopropylmalate dehydratase large subunit OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) GN=leuC PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.131e-10
Identity = 34/101 (33.66%), Postives = 59/101 (58.42%), Query Frame = 3

Query:  369 MTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLG 671
            MT+TEKILA  + K  + PGE +   +D+++ +DV  P    I + E      V+D+E+VV++ DH++   D ++     I R+F  + +I + YD+ D+G
Sbjct:    3 MTITEKILAAHAGKASVEPGELIQAKLDLVLANDVTAP--VSIKELEKASLDAVFDKERVVLVQDHFVPAKDIKSAEQSKIVRDFARKHDITHHYDVGDMG 101

[Close]

BLAST of CV091064 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0010s07560.1 ()

HSP 1 Score: 280.411 bits (716), Expect = 7.511e-76
Identity = 137/166 (82.53%), Postives = 149/166 (89.76%), Query Frame = 3

Query:  219 QKDVGLSAFSSQ-PS-FSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            +KD GLSAFSS  PS FSI   KK +S+KI  VMAPQQ+ER+PAT+GSVKTA+TMTEKI ARASEK QL PGENVWVNVD+LMTHDVCGPG+ GIFKKEFGQ+AKVWDREKVVIIPDHYIFT D+RANRNVDI R+FC EQNIKYFYDIKDLGNFKANPDYKGVCH
Sbjct:   16 KKDPGLSAFSSSSPSPFSIPKCKKSISKKIVSVMAPQQSERRPATTGSVKTALTMTEKIFARASEKPQLIPGENVWVNVDILMTHDVCGPGSIGIFKKEFGQDAKVWDREKVVIIPDHYIFTKDERANRNVDILRDFCNEQNIKYFYDIKDLGNFKANPDYKGVCH 181

[Close]

BLAST of CV091064 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0008s17240.1 ()

HSP 1 Score: 158.303 bits (399), Expect = 4.305e-39
Identity = 84/138 (60.87%), Postives = 99/138 (71.74%), Query Frame = 3

Query:  219 QKDVGLSAFSSQ---PSFSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREF 623
            +KD+GLSAFSS    P FS    KK VS+KI  VMAPQQ+ER PAT+GSVKTAMTMTEKI ARASE+ Q+SPGENVWVNVDVLMTHD+ GPG+ GIFK+EFG +AK        +       T+ +   +NV  CREF
Sbjct:   16 KKDLGLSAFSSSSSSPPFSAPKCKKSVSKKIVSVMAPQQSERMPATTGSVKTAMTMTEKIFARASEEPQVSPGENVWVNVDVLMTHDISGPGSIGIFKREFGPDAKGTCDMDTNVANHQTWLTSPEYGYKNVGSCREF 153

[Close]

BLAST of CV091064 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0008s17230.1 ()

HSP 1 Score: 117.857 bits (294), Expect = 6.447e-27
Identity = 51/61 (83.61%), Postives = 56/61 (91.80%), Query Frame = 3

Query:  528 VWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            VWDREKVV+IPDHYIFT D  ANRNVDI R+FC +Q+IKYFYDIKDLGN+KANPDYKGVCH
Sbjct:    1 VWDREKVVVIPDHYIFTEDKLANRNVDILRDFCNDQDIKYFYDIKDLGNYKANPDYKGVCH 61

[Close]

BLAST of CV091064 vs. P. persica 1.0 peptide
Match: ppa004448m ()

HSP 1 Score: 276.174 bits (705), Expect = 9.994e-75
Identity = 133/164 (81.10%), Postives = 145/164 (88.41%), Query Frame = 3

Query:  219 QKDVGLSAFSSQPSFSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            +KD+GLSAFSS    S +  K R S+ I  V AP+Q+ERKPAT+GSVKT MTMTEKI ARASEK QLSPGENVWVNVDVLMTHDVCGPG+FGIFKKEFGQNAKVWDREK+VIIPDHYIFT+D+RANRNVDI R+FC EQNIKYFYDIKDL NFKANPDYKGVCH
Sbjct:   18 KKDLGLSAFSSASPLSSQKCK-RTSKTICSVTAPKQSERKPATTGSVKTGMTMTEKIFARASEKTQLSPGENVWVNVDVLMTHDVCGPGSFGIFKKEFGQNAKVWDREKIVIIPDHYIFTSDERANRNVDILRDFCTEQNIKYFYDIKDLSNFKANPDYKGVCH 180

[Close]

BLAST of CV091064 vs. TrEMBL
Match: D7TL65|D7TL65_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_56.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029978001 PE=4 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 2.739e-76
Identity = 137/166 (82.53%), Postives = 151/166 (90.96%), Query Frame = 3

Query:  216 VQKDVGLSAFSSQPS-FSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            ++KDVGLSAFSS PS FS+   K+R SRKI  VMAPQQ+ R P+T+GSVKTAMTMTEKILARAS+K QLSPGENVWV+VD+LMTHDVCGPG+FGIFKKEFGQNAKVWDREK+VIIPDHYIFT D+RANRNVDI R+FC EQNIKYFYDIKDLGNFK NPDYKGVCH
Sbjct:   14 IKKDVGLSAFSSSPSSFSVPKTKRRNSRKIVSVMAPQQSPRSPSTTGSVKTAMTMTEKILARASQKPQLSPGENVWVDVDILMTHDVCGPGSFGIFKKEFGQNAKVWDREKIVIIPDHYIFTNDERANRNVDILRDFCTEQNIKYFYDIKDLGNFKVNPDYKGVCH 179

[Close]

BLAST of CV091064 vs. TrEMBL
Match: D7SZP6|D7SZP6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_49.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027922001 PE=4 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.319e-75
Identity = 138/166 (83.13%), Postives = 150/166 (90.36%), Query Frame = 3

Query:  216 VQKDVGLSAFSSQPS-FSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            ++KDV LSAFSS  S FSI+   KR SRKI  VMAPQQ+ R P+TSGSVKTAMTMTEKILARASEK QLSPGENVWVNVD+LMTHDVCGPG+FGIFKKEFG+NAKVWDREK+VIIP+HYIFT D+RANRNVDI REFC EQNIKYFYDIKDLGNF+ANPDYKGVCH
Sbjct:   14 IKKDVDLSAFSSSASSFSIQKTNKRNSRKIVSVMAPQQSPRSPSTSGSVKTAMTMTEKILARASEKPQLSPGENVWVNVDLLMTHDVCGPGSFGIFKKEFGENAKVWDREKIVIIPEHYIFTKDERANRNVDILREFCTEQNIKYFYDIKDLGNFRANPDYKGVCH 179

[Close]

BLAST of CV091064 vs. TrEMBL
Match: B9SQS5|B9SQS5_RICCO (3-isopropylmalate dehydratase, putative OS=Ricinus communis GN=RCOM_1217310 PE=4 SV=1)

HSP 1 Score: 285.419 bits (729), Expect = 3.029e-75
Identity = 135/166 (81.33%), Postives = 149/166 (89.76%), Query Frame = 3

Query:  219 QKDVGLSAFSSQ--PSFSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            +KD+GLSAFSSQ  P +SI   KK +S+K+  VMAPQQ+ER PAT+GSVKTAMTMTEKILARASEK QLSPGENVWVNVDVLMTHDVCGPG+ GIFK+EFGQNA VWDREK+VIIPDHYIFTTD+RANRNVDI R+FC EQN+KYFYDIKDL NFK NPDYKGVCH
Sbjct:   16 KKDLGLSAFSSQSSPFYSIPKCKKSISKKVVSVMAPQQSERTPATTGSVKTAMTMTEKILARASEKSQLSPGENVWVNVDVLMTHDVCGPGSIGIFKREFGQNATVWDREKIVIIPDHYIFTTDERANRNVDILRDFCQEQNVKYFYDIKDLSNFKVNPDYKGVCH 181

[Close]

BLAST of CV091064 vs. TrEMBL
Match: B9SQS6|B9SQS6_RICCO (3-isopropylmalate dehydratase, putative OS=Ricinus communis GN=RCOM_1217320 PE=4 SV=1)

HSP 1 Score: 256.529 bits (654), Expect = 1.507e-66
Identity = 123/166 (74.10%), Postives = 140/166 (84.34%), Query Frame = 3

Query:  219 QKDVGLSAFSS-QPSFSIRNAKKRVSRKITCVMAPQQTERKPATSGSV-KTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            QKD+GLS  SS  PS  +   KK + ++I  V++PQQ+ERKPAT+GSV K AMT TEKILARASEK QL PGENVWVNVD+LMTHDVCGPG+F IFK+EFG+NAKVWD EKVV+IPDHYIFT D  ANRNVDI REFC+EQNIK+FYDIKD+ NFKAN DYKGVCH
Sbjct:  153 QKDLGLSRLSSPSPSVYVLKCKKSIFKRIVAVVSPQQSERKPATTGSVVKNAMTTTEKILARASEKPQLGPGENVWVNVDILMTHDVCGPGSFAIFKREFGENAKVWDHEKVVVIPDHYIFTADKLANRNVDILREFCLEQNIKHFYDIKDISNFKANSDYKGVCH 318

[Close]

BLAST of CV091064 vs. TrEMBL
Match: B9HSU8|B9HSU8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_821804 PE=4 SV=1)

HSP 1 Score: 253.062 bits (645), Expect = 1.666e-65
Identity = 117/133 (87.97%), Postives = 126/133 (94.74%), Query Frame = 3

Query:  312 MAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            MAPQQ+ER+PAT+GSVKTA+TMTEKI ARASEK QL PGENVWVNVD+LMTHDVCGPG+ GIFKKEFGQ+AKVWDREKVVIIPDHYIFT D+RANRNVDI R+FC EQNIKYFYDIKDLGNFKANPDYKGVCH
Sbjct:    1 MAPQQSERRPATTGSVKTALTMTEKIFARASEKPQLIPGENVWVNVDILMTHDVCGPGSIGIFKKEFGQDAKVWDREKVVIIPDHYIFTKDERANRNVDILRDFCNEQNIKYFYDIKDLGNFKANPDYKGVCH 133

[Close]

BLAST of CV091064 vs. TrEMBL
Match: D7MV68|D7MV68_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_682975 PE=4 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 7.738e-63
Identity = 116/164 (70.73%), Postives = 132/164 (80.49%), Query Frame = 3

Query:  219 QKDVGLSAFSSQPSFSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            +KD+G+S+F      SI   +++    I  VMAPQ+ +R P T+GSVKT MTMTEKILARASEK  + PG+N+WVNVDVLMTHDVCGPG FGIFK+EFG  AKVWD EK+V+IPDHYIFT D RANRNVDI RE C EQNIKYFYDI DLGNFKANPDYKGVCH
Sbjct:   20 KKDLGISSFPKSSQISIHRCQRK---SIVSVMAPQK-DRSPGTTGSVKTGMTMTEKILARASEKSLVVPGDNIWVNVDVLMTHDVCGPGAFGIFKREFGDKAKVWDPEKIVVIPDHYIFTADKRANRNVDIMREHCREQNIKYFYDITDLGNFKANPDYKGVCH 179

[Close]

BLAST of CV091064 vs. TrEMBL
Match: C5XT62|C5XT62_SORBI (Putative uncharacterized protein Sb04g002120 OS=Sorghum bicolor GN=Sb04g002120 PE=4 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 6.551e-62
Identity = 110/138 (79.71%), Postives = 125/138 (90.58%), Query Frame = 3

Query:  297 KITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            ++  V +P +  R P+++GSVK+AMTMTEKILARASE+  L PGENVWV+VDVLMTHDVCGPGT GIFKKEFG++AKVWDREKVVIIPDHYIFTTD+RANRNVDI R+FC+EQNIKYFYDIKDL NFKANPDYKGVCH
Sbjct:   41 RVRAVASPARAPRAPSSTGSVKSAMTMTEKILARASERAALEPGENVWVDVDVLMTHDVCGPGTIGIFKKEFGEDAKVWDREKVVIIPDHYIFTTDERANRNVDILRDFCLEQNIKYFYDIKDLSNFKANPDYKGVCH 178

[Close]

BLAST of CV091064 vs. TrEMBL
Match: Q6Z702|Q6Z702_ORYSJ (Os02g0125100 protein OS=Oryza sativa subsp. japonica GN=P0575F10.36 PE=2 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 8.555e-62
Identity = 110/146 (75.34%), Postives = 128/146 (87.67%), Query Frame = 3

Query:  273 NAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            + ++  S ++  V  P +  R P+++GSVK+AMTMTEKILARASE+  L PGENVWV+VDVLMTHDVCGPGT GIFK+EFG++AKVWDREKVVIIPDHYIFT+D+RANRNVDI R+FCMEQNIKYFYDIKDL NFKANPDYKGVCH
Sbjct:   40 SGRRARSGRVRAVATPARAPRAPSSTGSVKSAMTMTEKILARASERASLEPGENVWVDVDVLMTHDVCGPGTIGIFKREFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYFYDIKDLSNFKANPDYKGVCH 185

[Close]

BLAST of CV091064 vs. TrEMBL
Match: B8AGY8|B8AGY8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_05669 PE=4 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 8.555e-62
Identity = 110/146 (75.34%), Postives = 128/146 (87.67%), Query Frame = 3

Query:  273 NAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            + ++  S ++  V  P +  R P+++GSVK+AMTMTEKILARASE+  L PGENVWV+VDVLMTHDVCGPGT GIFK+EFG++AKVWDREKVVIIPDHYIFT+D+RANRNVDI R+FCMEQNIKYFYDIKDL NFKANPDYKGVCH
Sbjct:   40 SGRRARSGRVRAVATPARAPRAPSSTGSVKSAMTMTEKILARASERASLEPGENVWVDVDVLMTHDVCGPGTIGIFKREFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCMEQNIKYFYDIKDLSNFKANPDYKGVCH 185

[Close]

BLAST of CV091064 vs. TrEMBL
Match: C4JBG7|C4JBG7_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 1.906e-61
Identity = 108/138 (78.26%), Postives = 126/138 (91.30%), Query Frame = 3

Query:  297 KITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            ++  V +P +T R P+++GSVKTAMTMTEKILARASE+  L PGENVWV++DVLMTHDVCGPGT GIFKKEFG++AKVWDREKVVIIPDHYIFT+D+RANRNVDI R+FC+EQNIKYFYDIKDL +F+ANPDYKGVCH
Sbjct:   39 RVRAVASPARTPRAPSSTGSVKTAMTMTEKILARASERAALEPGENVWVDIDVLMTHDVCGPGTIGIFKKEFGEDAKVWDREKVVIIPDHYIFTSDERANRNVDILRDFCLEQNIKYFYDIKDLSDFRANPDYKGVCH 176

[Close]

BLAST of CV091064 vs. Vitis vinifera peptide
Match: GSVIVT01029978001 (assembled CDS)

HSP 1 Score: 288.886 bits (738), Expect = 1.272e-78
Identity = 137/166 (82.53%), Postives = 151/166 (90.96%), Query Frame = 3

Query:  216 VQKDVGLSAFSSQPS-FSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            ++KDVGLSAFSS PS FS+   K+R SRKI  VMAPQQ+ R P+T+GSVKTAMTMTEKILARAS+K QLSPGENVWV+VD+LMTHDVCGPG+FGIFKKEFGQNAKVWDREK+VIIPDHYIFT D+RANRNVDI R+FC EQNIKYFYDIKDLGNFK NPDYKGVCH
Sbjct:   14 IKKDVGLSAFSSSPSSFSVPKTKRRNSRKIVSVMAPQQSPRSPSTTGSVKTAMTMTEKILARASQKPQLSPGENVWVDVDILMTHDVCGPGSFGIFKKEFGQNAKVWDREKIVIIPDHYIFTNDERANRNVDILRDFCTEQNIKYFYDIKDLGNFKVNPDYKGVCH 179

[Close]

BLAST of CV091064 vs. Vitis vinifera peptide
Match: GSVIVT01027922001 (assembled CDS)

HSP 1 Score: 285.804 bits (730), Expect = 1.076e-77
Identity = 138/166 (83.13%), Postives = 150/166 (90.36%), Query Frame = 3

Query:  216 VQKDVGLSAFSSQPS-FSIRNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            ++KDV LSAFSS  S FSI+   KR SRKI  VMAPQQ+ R P+TSGSVKTAMTMTEKILARASEK QLSPGENVWVNVD+LMTHDVCGPG+FGIFKKEFG+NAKVWDREK+VIIP+HYIFT D+RANRNVDI REFC EQNIKYFYDIKDLGNF+ANPDYKGVCH
Sbjct:   14 IKKDVDLSAFSSSASSFSIQKTNKRNSRKIVSVMAPQQSPRSPSTSGSVKTAMTMTEKILARASEKPQLSPGENVWVNVDLLMTHDVCGPGSFGIFKKEFGENAKVWDREKIVIIPEHYIFTKDERANRNVDILREFCTEQNIKYFYDIKDLGNFRANPDYKGVCH 179

[Close]

BLAST of CV091064 vs. TAIR peptide
Match: AT4G13430.1 (| Symbols: IIL1, ATLEUC1 | isopropyl malate isomerase large subunit 1 | chr4:7804194-7807789 REVERSE LENGTH=509)

HSP 1 Score: 252.292 bits (643), Expect = 1.927e-67
Identity = 121/165 (73.33%), Postives = 136/165 (82.42%), Query Frame = 3

Query:  219 QKDVGLSAFSSQPSFSI-RNAKKRVSRKITCVMAPQQTERKPATSGSVKTAMTMTEKILARASEKQQLSPGENVWVNVDVLMTHDVCGPGTFGIFKKEFGQNAKVWDREKVVIIPDHYIFTTDDRANRNVDICREFCMEQNIKYFYDIKDLGNFKANPDYKGVCH 710
            + D+G+S+F      SI R  KK +SRKI  VMAPQ+ +R P T+GSVKT MTMTEKILARASEK  + PG+N+WVNVDVLMTHDVCGPG FGIFK+EFG+ AKVWD EK+V+IPDHYIFT D RANRNVDI RE C EQNIKYFYDI DLGNFKANPDYKGVCH
Sbjct:   17 KSDLGISSFPKSSQISIHRCQKKSISRKIVSVMAPQK-DRSPGTTGSVKTGMTMTEKILARASEKSLVVPGDNIWVNVDVLMTHDVCGPGAFGIFKREFGEKAKVWDPEKIVVIPDHYIFTADKRANRNVDIMREHCREQNIKYFYDITDLGNFKANPDYKGVCH 180

The following BLAST results are available for this feature:

BLAST of CV091064 vs. DB:swissprot:display
BLAST of CV091064 vs. Populus trichocarpa peptide v2.0
BLAST of CV091064 vs. P. persica 1.0 peptide
BLAST of CV091064 vs. TrEMBL
BLAST of CV091064 vs. Vitis vinifera peptide
BLAST of CV091064 vs. TAIR peptide

BLAST of CV091064 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LEUC_ARATH	1.899e-66	73.33	3-isopropylmalate dehydratase OS=Arabidopsis thali...	[more]
LEUC_METJA	1.129e-18	45.61	Isopropylmalate/citramalate isomerase large subuni...	[more]
LEUC1_METTH	6.848e-16	41.74	3-isopropylmalate dehydratase large subunit 1 OS=M...	[more]
LEUC_SYNAS	3.181e-13	36.61	3-isopropylmalate dehydratase large subunit OS=Syn...	[more]
LEUC1_METKA	2.062e-12	39.82	3-isopropylmalate dehydratase large subunit 1 OS=M...	[more]
LEUC_DESAA	5.999e-12	33.04	3-isopropylmalate dehydratase large subunit OS=Des...	[more]
LEUC1_ARCFU	1.745e-11	38.38	3-isopropylmalate dehydratase large subunit 1 OS=A...	[more]
LEUC_THEYD	8.662e-11	32.67	3-isopropylmalate dehydratase large subunit OS=The...	[more]
LEUC_CALS8	8.662e-11	32.67	3-isopropylmalate dehydratase large subunit OS=Cal...	[more]
LEUC_HELMI	1.131e-10	33.66	3-isopropylmalate dehydratase large subunit OS=Hel...	[more]

BLAST of CV091064 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 3


Match Name	E-value	Identity
POPTR_0010s07560.1	7.511e-76	82.53	[more]
POPTR_0008s17240.1	4.305e-39	60.87	[more]
POPTR_0008s17230.1	6.447e-27	83.61	[more]

BLAST of CV091064 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ppa004448m	9.994e-75	81.10		[more]

BLAST of CV091064 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
D7TL65\|D7TL65_VITVI	2.739e-76	82.53	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7SZP6\|D7SZP6_VITVI	2.319e-75	83.13	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9SQS5\|B9SQS5_RICCO	3.029e-75	81.33	3-isopropylmalate dehydratase, putative OS=Ricinus...	[more]
B9SQS6\|B9SQS6_RICCO	1.507e-66	74.10	3-isopropylmalate dehydratase, putative OS=Ricinus...	[more]
B9HSU8\|B9HSU8_POPTR	1.666e-65	87.97	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7MV68\|D7MV68_ARALY	7.738e-63	70.73	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
C5XT62\|C5XT62_SORBI	6.551e-62	79.71	Putative uncharacterized protein Sb04g002120 OS=So...	[more]
Q6Z702\|Q6Z702_ORYSJ	8.555e-62	75.34	Os02g0125100 protein OS=Oryza sativa subsp. japoni...	[more]
B8AGY8\|B8AGY8_ORYSI	8.555e-62	75.34	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C4JBG7\|C4JBG7_MAIZE	1.906e-61	78.26	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of CV091064 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 2


Match Name	E-value	Identity	Description
GSVIVT01029978001	1.272e-78	82.53	assembled CDS	[more]
GSVIVT01027922001	1.076e-77	83.13	assembled CDS	[more]

BLAST of CV091064 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G13430.1	1.927e-67	73.33	\| Symbols: IIL1, ATLEUC1 \| isopropyl malate isomer...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001030	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha	SUPERFAMILY	53732	Aconitase iron-sulfur domain	coord: 62..184 score: 2.3
IPR015931	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3	GENE3D	3.30.499.10	no description	coord: 67..166 score: 1.9
IPR015936	Homoaconitase/3-isopropylmalate dehydratase, small/large subunit	PANTHER	PTHR11670:SF6	3-ISOPROPYLMALATE DEHYDRATASE	coord: 37..184 score: 2.8
IPR015937	Aconitase/isopropylmalate dehydratase	PANTHER	PTHR11670	ACONITASE	coord: 37..184 score: 2.8
None	No IPR available	SEG	seg	seg	coord: 2..18 score

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV091064 ID=CV091064; Name=CV091064; organism=Vaccinium corymbosum; type=EST; length=712bp
GTTTTTCCTAGCACTGGCCCATAGTGCGAAGAAGACCGATAATTTCCTCA
GTCTCCAGTCTCCATTGCATTGATACTACGCTATAGCTTATTACTCTGCA
ACTCGCGTTCTCCTCTCACCAGGTCCACACAATACAGACGGCTAATTTCA
CAACCTGAAAACCCGCAATGGCTTCATCTGCTATTGCACCGAGCTCCTCA
CCTTTCATCAGCAAGGTTCAGAAAGATGTGGGCCTCTCTGCTTTCTCCTC
ACAACCCTCATTTTCCATACGCAATGCCAAGAAAAGAGTTTCCAGGAAGA
TCACGTGCGTCATGGCGCCACAACAAACTGAACGCAAGCCCGCCACCTCT
GGTTCAGTCAAGACTGCAATGACGATGACCGAGAAGATCCTGGCTAGGGC
TTCAGAGAAACAGCAGCTGAGCCCAGGCGAGAATGTATGGGTCAACGTGG
ATGTCTTGATGACTCATGATGTTTGTGGACCTGGCACTTTTGGAATCTTC
AAGAAAGAGTTTGGACAAAATGCTAAGGTGTGGGACCGTGAAAAGGTTGT
GATCATACCTGACCACTATATATTCACTACTGACGACCGAGCGAATCGCA
ACGTGGATATCTGCAGGGAATTTTGCATGGAACAAAATATAAAGTACTTT
TATGATATCAAAGATCTTGGGAATTTCAAGGCTAACCCTGATTATAAAGG
GGTATGCCATGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0008652	amino acid biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016836	hydro-lyase activity

Vocabulary: **KEGG ORTHOLOGY**
Term	Definition
K01703	leuC; 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC:4.2.1.33 4.2.1.35]

Vocabulary: **KEGG PATHWAY**
Term	Definition
ko00010	Glycolysis / Gluconeogenesis
ko00250	Alanine, aspartate and glutamate metabolism

Vocabulary: **INTERPRO**
Term	Definition
IPR001030	Acoase/IPM_deHydtase_lsu_aba
IPR015931	Acnase/IPM_dHydase_lsu_aba_1/3
IPR015936	Homoaconitase/3-isopropylmalate dehydratase, small/large subunit
IPR015937	Aconitase/isopropylmalate dehydratase
noIPR	unintegrated