CV191070 | GDV

EST Overview


Name	CV191070
Unique Name	CV191070
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	650

Libraries


Library Name	Type
LIBEST_016374 Vco01	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18
KEGG analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

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BLAST of CV191070 vs. DB:swissprot:display
Match: RFA1_SCHPO (Replication factor A protein 1 OS=Schizosaccharomyces pombe GN=ssb1 PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 7.688e-16
Identity = 50/146 (34.25%), Postives = 80/146 (54.79%), Query Frame = 3

Query:    9 GAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVI--LDKCDVIGEPKHFDRPGSNTNSAPAARSSSQVQSNVDQPGTMTSNA 440
            GA+ ++++ DA      P++QV+ ++ +N+   +   +RYRV+LSD +N  Q +L+TQ N LV    LQKG+ VQL++F  N++  R  +IV+ LNV+  L   D IG P       +   +  A R     Q+N   P T  S +
Sbjct:    8 GALRIINTSDASSFPPNPILQVLTVKELNSNPTSGAPKRYRVVLSDSINYAQSMLSTQLNHLVAENKLQKGAFVQLTQFTVNVMKERKILIVLGLNVLTELGVMDKIGNP-------AGLETVDALRQQQNEQNNASAPRTGISTS 146

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BLAST of CV191070 vs. DB:swissprot:display
Match: RFA1_XENLA (Replication protein A 70 kDa DNA-binding subunit OS=Xenopus laevis GN=rpa1 PE=1 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.315e-12
Identity = 56/152 (36.84%), Postives = 87/152 (57.24%), Query Frame = 3

Query:    3 AEGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVN-LQQGILATQKNDLVHTQALQKGSIVQLSEFVC-NLILGRTSIIVIDLNVILDKCDV----IGEPKHFD----RPGSNTNSAPAARSSSQVQSNVDQPGTM 428
            +EGAI+ +  GD+     KP +QV+++R +NT N      RYR+L+SDG+N L   +LATQ N LV    L    I Q+S F+  NL  GR  IIV++L+V L   D+    IG P+ ++    +P +   ++  A + S++Q+N   P +M
Sbjct:    7 SEGAISAMLGGDSS---CKPTLQVINIRPINTGN---GPPRYRLLMSDGLNTLSSFMLATQLNSLVDNNLLATNCICQVSRFIVNNLKDGRRVIIVMELDV-LKSADLVMGKIGNPQPYNDGQPQPAAPAPASAPAPAPSKLQNNSAPPPSM 151

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BLAST of CV191070 vs. DB:swissprot:display
Match: RFA1_PONAB (Replication protein A 70 kDa DNA-binding subunit OS=Pongo abelii GN=RPA1 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.735e-12
Identity = 53/153 (34.64%), Postives = 84/153 (54.90%), Query Frame = 3

Query:    3 AEGAIA-MLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVN-LQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLIL-GRTSIIVIDLNVILDKCDV----IGEPKHFDRPGSNTNSAPAARSSSQVQSNVDQPGTMTSNA 440
            +EGAIA ++  GD    ++KP++QV+++R + T N      RYR+L+SDG+N L   + ATQ N LV  + L    + Q++ F+ N +  GR  +I+++L V L   +V    IG P  ++        AP A ++S   S+  QP   TS A
Sbjct:    6 SEGAIAAIMQKGDT---NIKPILQVINIRPITTGN---SPPRYRLLMSDGLNTLSSFMSATQLNPLVEQEQLSSNCVCQINRFIVNTLKDGRRVVILMELEV-LKSAEVVGVKIGNPVPYNEGLGQPQVAPPAPAASPAASSRPQPQNGTSGA 151

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BLAST of CV191070 vs. DB:swissprot:display
Match: RFA1_HUMAN (Replication protein A 70 kDa DNA-binding subunit OS=Homo sapiens GN=RPA1 PE=1 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 1.500e-11
Identity = 50/145 (34.48%), Postives = 80/145 (55.17%), Query Frame = 3

Query:    3 AEGAIA-MLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVN-LQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLIL-GRTSIIVIDLNVILDKCDV---IGEPKHFDRPGSNTNSAPAARSSSQVQSNVDQP 419
            +EGAIA ++  GD    ++KP++QV+++R + T N      RYR+L+SDG+N L   +LATQ N LV  + L    + Q+  F+ N +  GR  +I+++L V+     V   IG P  ++        AP A ++S   S+  QP
Sbjct:    6 SEGAIAAIMQKGDT---NIKPILQVINIRPITTGN---SPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELEVLKSAEAVGVKIGNPVPYNEGLGQPQVAPPAPAASPAASSRPQP 144

[Close]

BLAST of CV191070 vs. DB:swissprot:display
Match: RFA1_CHICK (Replication protein A 70 kDa DNA-binding subunit OS=Gallus gallus GN=RPA1 PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 4.365e-11
Identity = 53/136 (38.97%), Postives = 77/136 (56.62%), Query Frame = 3

Query:    3 AEGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVN-LQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLIL-GRTSIIVIDLNVILDKCDV----IGEPKHFDR-PGSNTNSAPAARSS 389
            +EGAIA +  G   E   KPV+QV++ R + T N      RYRVL+SDGVN L   +LATQ N LV  + L    I Q++ F+ N +  GR  +I++DL+V L   D+    +G P  ++   G   +SAP A ++
Sbjct:    6 SEGAIAAIMQG---ENVYKPVLQVINTRAIATGNGPP---RYRVLMSDGVNTLSSFMLATQLNPLVEEERLSAHCICQVNRFIVNSLKDGRRVVILMDLDV-LKTADMVGGTVGNPVPYNEGQGQQRSSAPTANAA 134

[Close]

BLAST of CV191070 vs. DB:swissprot:display
Match: RFA1_XENTR (Replication protein A 70 kDa DNA-binding subunit OS=Xenopus tropicalis GN=rpa1 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 9.725e-11
Identity = 54/136 (39.71%), Postives = 76/136 (55.88%), Query Frame = 3

Query:    3 AEGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVN-LQQGILATQKNDLVHTQALQKGSIVQLSEFVC-NLILGRTSIIVIDLNVILDKCDV----IGEPKHF-DRPGSNTNSAPAARSS 389
            +EGAI+ +  GD+     KP +QV+++R +NT N      RYR+L+SDG+N L   +LATQ N LV    L    I Q+S F+  NL  GR  IIV+++ V L   D+    IG P  + D  G    +APA  S+
Sbjct:    7 SEGAISAILGGDSS---CKPTLQVINIRSINTGN---GPPRYRLLMSDGLNTLSSFMLATQLNFLVDNNLLATNCICQVSRFIVNNLKDGRRVIIVMEMEV-LKSADLVKGKIGNPHPYNDGQGPPQPAAPAPASA 135

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BLAST of CV191070 vs. DB:swissprot:display
Match: RFA1_MOUSE (Replication protein A 70 kDa DNA-binding subunit OS=Mus musculus GN=Rpa1 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.404e-9
Identity = 41/115 (35.65%), Postives = 69/115 (60.00%), Query Frame = 3

Query:    3 AEGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVN-LQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLIL-GRTSIIVIDLNVILDKCDV---IGEP 332
            +EGAI ++     +   +KP++QV+++R ++T N   ++ RYR+L+SDG+N L   +LATQ N LV    L    + Q+ +F+ N +  GR  ++++DL V+    DV   IG P
Sbjct:    6 SEGAIEVMIQ--QENTSIKPILQVINIRPISTGN---RSPRYRLLMSDGLNTLSSFMLATQLNTLVEGGQLASNCVCQVHKFIVNTLKDGRKVVVLMDLEVMKSAEDVGLKIGNP 115

[Close]

BLAST of CV191070 vs. DB:swissprot:display
Match: RFA1_DROME (Replication protein A 70 kDa DNA-binding subunit OS=Drosophila melanogaster GN=RpA-70 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 6.969e-9
Identity = 44/150 (29.33%), Postives = 79/150 (52.67%), Query Frame = 3

Query:    9 GAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQ-GILATQKNDLVHTQALQKGSIVQLSEFVCNLI----LGRTSIIVIDLNVILDKCDV---IGEPKHFD---------RPGSNTNSAPAARSSSQVQSN 407
            G IA +  G+  +A   PV+Q++ ++ +N+      +ERYR+L+SDG       +LA+Q N + H   L++ +IVQL ++V +L+     G+  +I+ +L V+    +V   IGEP  ++         +P   +NS P A+      +N
Sbjct:    9 GVIARIMHGEVVDA---PVLQILAIKKINSA---ADSERYRILISDGKYFNSYAMLASQLNVMQHNGELEEFTIVQLDKYVTSLVGKDGAGKRVLIISELTVVNPGAEVKSKIGEPVTYENAAKQDLAPKPAVTSNSKPIAKKEPSHNNN 152

[Close]

BLAST of CV191070 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0003s10170.1 ()

HSP 1 Score: 109.383 bits (272), Expect = 1.985e-24
Identity = 61/144 (42.36%), Postives = 91/144 (63.19%), Query Frame = 3

Query:    6 EGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPGSNTNSAPAARSSSQVQSNVDQPGTMTSN 437
            EGAI+ ++SG A  A+LKP +QV DL+ V T+ Q QQ++R+R++LSDG +LQQ +L TQ N LV    L+ GSIVQL+++ C  + GR  II+++L ++ + C++IG P              A RSS    S V QPG + ++
Sbjct:    5 EGAISKITSGGATSAELKPTLQVTDLKQVQTK-QPQQSDRFRLVLSDGSHLQQAMLGTQINHLVKDGNLRIGSIVQLNQYTCTTVQGRMIIIILELALVAEDCELIGNP------------VSAQRSSGPSPSPVAQPGNVNAS 135

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BLAST of CV191070 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0018s07150.1 ()

HSP 1 Score: 87.8113 bits (216), Expect = 6.183e-18
Identity = 50/130 (38.46%), Postives = 81/130 (62.31%), Query Frame = 3

Query:   12 AIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHF-DRPGS----NTNSAPAARS 386
            AIAM++ GD    +LKP+VQV+D++ + +       ER+R+L+SD V  Q  +L TQ ND V T  ++KGS++QL +++C+ +  R  I+V++L  I+  C++IG PK   D P      NT+S  ++ S
Sbjct:    9 AIAMINGGDV---NLKPLVQVLDIKQMGSAQ-----ERFRLLVSDSVLTQHAMLGTQLNDRVKTGLVKKGSVIQLIDYICSQVQKRKIIVVLNLETIIPDCEIIGNPKMVSDAPAQKALPNTDSMQSSMS 130

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BLAST of CV191070 vs. P. persica 1.0 peptide
Match: ppa002611m ()

HSP 1 Score: 90.1225 bits (222), Expect = 8.808e-19
Identity = 46/120 (38.33%), Postives = 75/120 (62.50%), Query Frame = 3

Query:   12 AIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPGSNTNSA 371
            AIA +S+GD      KP+VQV+D++L+ TQ      ERYR ++SD V+ Q  ++ATQ ND + T  ++KGSI+QL++++ N       I+V+++  I+   ++IG+PK +   GS    A
Sbjct:    9 AIAAISAGDLNS---KPLVQVIDIKLLGTQ------ERYRFIVSDAVSCQHAMVATQLNDRIKTSQVKKGSIIQLTDYISNYFQNHKIIVVLNMETIILDSEIIGDPKPYVESGSTAQKA 119

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BLAST of CV191070 vs. P. persica 1.0 peptide
Match: ppa001396m ()

HSP 1 Score: 85.8853 bits (211), Expect = 1.661e-17
Identity = 45/86 (52.33%), Postives = 58/86 (67.44%), Query Frame = 3

Query:    6 EGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQ----NERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVC 251
            EGAI  + +G+  +   KP++QV+D+R+VNT     Q    NERYRVL+SDG + QQG+L TQKN LV    LQKGSIV L +F C
Sbjct:    7 EGAIMKICTGEYHDETWKPILQVLDVRMVNTARSGAQPGPDNERYRVLISDGSHHQQGMLGTQKNTLVQQGLLQKGSIVCLKQFTC 92

[Close]

BLAST of CV191070 vs. TrEMBL
Match: A5AQN6|A5AQN6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038295 PE=4 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 1.766e-44
Identity = 98/182 (53.85%), Postives = 128/182 (70.33%), Query Frame = 3

Query:    6 EGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPGSNTNSAPAARSSSQVQSNVDQPGTMTSNAXXXXXXXXXXXXXXAKPNVIGGAFPIQP-KVDRSPDS 548
            EGAI+ML SGDAQ +D+KPV+QV D+RLVNTQ Q+  NER+R+LLSDG +LQQG+LATQ+N+LV +  LQKGS+VQL++FVCN+I  R  II+IDL+VIL+KCD IGEPK + + G+    AP AR+S+ + S+VD PGT+  N               AKPN+ G A P        +PDS
Sbjct:    7 EGAISMLCSGDAQGSDVKPVLQVADIRLVNTQQQSNSNERFRILLSDGCHLQQGMLATQRNELVKSGRLQKGSVVQLTQFVCNVIKDRMIIIIIDLDVILEKCDPIGEPKQYVQGGTAGGVAPIARTSAPMPSSVDHPGTVLGN-PQSFGNSSLTGGSAAKPNMAGVASPYHANSFSSNPDS 187

[Close]

BLAST of CV191070 vs. TrEMBL
Match: D7U3Y7|D7U3Y7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_33.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00022784001 PE=4 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 1.037e-36
Identity = 76/117 (64.96%), Postives = 99/117 (84.62%), Query Frame = 3

Query:    6 EGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPGS 356
            EGAI+ML SGDAQ +D+KPV+QV D+RLVNTQ Q+  NER+R+LLSDG +LQQG+LATQ+N+LV +  LQKGS+VQL++FVCN+I  R  II+IDL+VIL+KCD IGEPK + + G+
Sbjct:    7 EGAISMLCSGDAQGSDVKPVLQVADIRLVNTQQQSNSNERFRILLSDGCHLQQGMLATQRNELVKSGRLQKGSVVQLTQFVCNVIKDRMIIIIIDLDVILEKCDPIGEPKQYVQGGT 123

[Close]

BLAST of CV191070 vs. TrEMBL
Match: A9TFZ6|A9TFZ6_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_144964 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.582e-21
Identity = 57/149 (38.26%), Postives = 86/149 (57.72%), Query Frame = 3

Query:   12 AIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHF------DRPGSNTNSAPAARSSSQVQSNVDQPGTMTSNA 440
            AI  L++GD +   L+PV+Q+VD+R +   N     ER+R++LSDGV+LQQ +LATQ N+ V      KGSIVQL E++CN +  R  IIV+++ ++  K ++IG+PKH         PG      P +    Q    +  P + T +A
Sbjct:    9 AIVALNNGDVE---LRPVLQIVDVRQIG--NSQTTTERFRLVLSDGVHLQQAMLATQLNEKVKNNLAVKGSIVQLLEYICNTVQNRKIIIVLNMEIVETKAEIIGDPKHLIAGGEHQAPGPGPGPGPGSLQPQQPPPRISTPSSATVSA 152

[Close]

BLAST of CV191070 vs. TrEMBL
Match: A9TA55|A9TA55_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_11886 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.582e-21
Identity = 59/145 (40.69%), Postives = 86/145 (59.31%), Query Frame = 3

Query:   12 AIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPG---SNTNSAPAARSSSQVQSNVDQPGTMTSN 437
            AI  L++GD +   L+PV+Q+VD+R +   N     ER+R++LSDGV+LQQ +LATQ N+ V      KGSIVQL E++CN +  R  IIV+++ ++  K D+IG+PKH    G   +     P   SS   Q +   P   T+N
Sbjct:    9 AIVALNNGDVE---LRPVLQIVDIRQIG--NAQTTTERFRLVLSDGVHLQQAMLATQLNEKVKNNLAVKGSIVQLLEYICNTVQNRKIIIVLNMEIVESKADIIGDPKHLVAGGEHQAGPGPGPGPGSSQLQQPSPSLPKNTTAN 148

[Close]

BLAST of CV191070 vs. TrEMBL
Match: Q65XV7|Q65XV7_ORYSJ (Os05g0111000 protein OS=Oryza sativa subsp. japonica GN=P0016H04.11 PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.961e-20
Identity = 57/136 (41.91%), Postives = 85/136 (62.50%), Query Frame = 3

Query:    9 GAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQN---QQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPGSNTNSAPAARSSSQVQSN 407
            GA+  ++        ++PV+QVVD+R V T+N     +  ER+R++LSDGVN QQ +LAT  N LV    L+ G++VQL++F+CN I G+  IIV+ L+V+ + C VIG PKH++ P S T        +S  Q+N
Sbjct:    8 GAVQAIAEHPDGTGTIQPVLQVVDVRPVTTKNAPPTPKPAERFRMMLSDGVNTQQSMLATALNPLVKDATLRPGTVVQLTDFMCNTIQGKRIIIVVKLDVLQNDCIVIGNPKHYE-PKSLTKEQDPNLQASVAQTN 142

[Close]

BLAST of CV191070 vs. TrEMBL
Match: B8AWZ3|B8AWZ3_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_18166 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.961e-20
Identity = 57/136 (41.91%), Postives = 85/136 (62.50%), Query Frame = 3

Query:    9 GAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQN---QQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPGSNTNSAPAARSSSQVQSN 407
            GA+  ++        ++PV+QVVD+R V T+N     +  ER+R++LSDGVN QQ +LAT  N LV    L+ G++VQL++F+CN I G+  IIV+ L+V+ + C VIG PKH++ P S T        +S  Q+N
Sbjct:  129 GAVQAIAEHPDGTGTIQPVLQVVDVRPVTTKNAPPTPKPAERFRMMLSDGVNTQQSMLATALNPLVKDATLRPGTVVQLTDFMCNTIQGKRIIIVVKLDVLQNDCIVIGNPKHYE-PKSLTKEQDPNLQASVAQTN 263

[Close]

BLAST of CV191070 vs. TrEMBL
Match: Q9FHJ6|Q9FHJ6_ARATH (Replication protein A1-like OS=Arabidopsis thaliana GN=At5g45400 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 5.719e-19
Identity = 53/146 (36.30%), Postives = 91/146 (62.33%), Query Frame = 3

Query:    6 EGAIAMLSSGDA-QEADLKPVVQVVDLRLVNTQ-NQNQQNE-RYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPK-------HFDRPGSNTNSAPAARSSSQVQSNVD 413
            EG +  + +G+   E D+ PV+QV +L+L+ ++ +QNQ++  RY+ LLSDG +L  G+L T  N LV+   +Q GS+++L+ ++CNLI  R  ++++ L VI++KC++IG PK       +  R G NT S   +      +S+V+
Sbjct:    7 EGVVMKMLNGEVTSETDMMPVLQVTELKLIQSKLHQNQESSNRYKFLLSDGTDLAAGMLNTSLNSLVNQGTIQLGSVIRLTHYICNLIQTRRIVVIMQLEVIVEKCNIIGNPKEPGHSSINPQRGGVNTQSNGGSEQQQARRSDVN 152

[Close]

BLAST of CV191070 vs. TrEMBL
Match: D7KGL1|D7KGL1_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_313748 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 9.756e-19
Identity = 54/129 (41.86%), Postives = 82/129 (63.57%), Query Frame = 3

Query:    9 GAIAMLSSGD-AQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPGSNTNSAPAARSSS 392
            GAI  + +G+ + E DL PV+QV +L+++       Q+ RY+ LLSDG++LQ G+L T  N LV   ++Q GSIV+L+ +VC+LI GR  +I++ L VI+ KCD+IG P   + PG    +A    + S
Sbjct:    8 GAITKILNGEVSSEIDLMPVLQVTELKMI-------QSNRYKFLLSDGIHLQAGMLNTTLNSLVIQGSIQLGSIVRLTHYVCSLIQGRRIVIIMQLEVIVAKCDIIGTP---NEPGQRGGAAVPVNTQS 126

[Close]

BLAST of CV191070 vs. TrEMBL
Match: B6U4N8|B6U4N8_MAIZE (DNA binding protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 2.838e-18
Identity = 56/147 (38.10%), Postives = 86/147 (58.50%), Query Frame = 3

Query:    9 GAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFD---RPGSNTNSAPAARSSSQVQSNVDQPGTMTSNA 440
            GA+A +S+       L+PV+QVVD+R+VN   +N   ER+R++LSDG +  Q +LAT +N  V   ++QKGSIV L E  C+ I  R  IIVI L+V+  +C +IG P+ ++   +P     + PA  + +   +    PG + S A
Sbjct:    8 GAVAAVSAHADGNGTLQPVLQVVDVRMVNNA-KNPSAERFRMVLSDGEHTMQSMLATAENARVRDGSIQKGSIVHLLECTCSTIQSRRIIIVIKLDVLQSECAIIGNPRPYEMRNQPNEQGTNIPANAAQANTGTYSSGPGLLGSPA 153

[Close]

BLAST of CV191070 vs. TrEMBL
Match: B3VTC8|B3VTC8_SORBI (Putative uncharacterized protein Sb09g001260 OS=Sorghum bicolor GN=Sb09g001260 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 4.842e-18
Identity = 54/148 (36.49%), Postives = 85/148 (57.43%), Query Frame = 3

Query:    9 GAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFD---RPGSNT-NSAPAARSSSQVQSNVDQPGTMTSNA 440
            GA+A +S        L+PV+QVVD+R+V         ER+R+LLSDG +  Q +L+T +N  +    ++KGSIV L EF C+ I  R  II+I L+V+  +C +IG+PK +D   +P +    + PA  + +   +    PG + S+A
Sbjct:    8 GAVAAVSEHADGNGTLQPVLQVVDVRIVPNVKSATNQERFRMLLSDGTHTMQSMLSTSENARIRDGIIKKGSIVHLHEFTCSTIQHRRIIIIIKLDVLQTECAIIGDPKPYDVRIQPNNEQGTNIPANSAQANTGTYSSGPGMLGSSA 155

[Close]

BLAST of CV191070 vs. Vitis vinifera peptide
Match: GSVIVT01022784001 (assembled CDS)

HSP 1 Score: 157.147 bits (396), Expect = 5.014e-39
Identity = 76/117 (64.96%), Postives = 99/117 (84.62%), Query Frame = 3

Query:    6 EGAIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHFDRPGS 356
            EGAI+ML SGDAQ +D+KPV+QV D+RLVNTQ Q+  NER+R+LLSDG +LQQG+LATQ+N+LV +  LQKGS+VQL++FVCN+I  R  II+IDL+VIL+KCD IGEPK + + G+
Sbjct:    7 EGAISMLCSGDAQGSDVKPVLQVADIRLVNTQQQSNSNERFRILLSDGCHLQQGMLATQRNELVKSGRLQKGSVVQLTQFVCNVIKDRMIIIIIDLDVILEKCDPIGEPKQYVQGGT 123

[Close]

BLAST of CV191070 vs. Vitis vinifera peptide
Match: GSVIVT01015111001 (assembled CDS)

HSP 1 Score: 92.4337 bits (228), Expect = 1.517e-19
Identity = 45/108 (41.67%), Postives = 72/108 (66.67%), Query Frame = 3

Query:   12 AIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPK 335
            AIA ++ GD      KP+VQV+D++L+         ERYR LLSD V  QQ +LATQ ND V +  ++KGS++QL +++C+++  R  I+V+++  I+ +C++IG PK
Sbjct:    9 AIAAINGGDVNS---KPLVQVLDIKLIGNSQ-----ERYRFLLSDAVATQQAMLATQLNDRVKSGQVRKGSVIQLIDYICSVVQNRKIIVVLNMETIIPECEIIGNPK 108

[Close]

BLAST of CV191070 vs. TAIR peptide
Match: AT5G45400.1 (| Symbols: RPA70C, ATRPA70C | Replication factor-A protein 1-related | chr5:18398990-18401644 FORWARD LENGTH=853)

HSP 1 Score: 98.2117 bits (243), Expect = 4.027e-21
Identity = 53/146 (36.30%), Postives = 91/146 (62.33%), Query Frame = 3

Query:    6 EGAIAMLSSGDA-QEADLKPVVQVVDLRLVNTQ-NQNQQNE-RYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPK-------HFDRPGSNTNSAPAARSSSQVQSNVD 413
            EG +  + +G+   E D+ PV+QV +L+L+ ++ +QNQ++  RY+ LLSDG +L  G+L T  N LV+   +Q GS+++L+ ++CNLI  R  ++++ L VI++KC++IG PK       +  R G NT S   +      +S+V+
Sbjct:    7 EGVVMKMLNGEVTSETDMMPVLQVTELKLIQSKLHQNQESSNRYKFLLSDGTDLAAGMLNTSLNSLVNQGTIQLGSVIRLTHYICNLIQTRRIVVIMQLEVIVEKCNIIGNPKEPGHSSINPQRGGVNTQSNGGSEQQQARRSDVN 152

[Close]

BLAST of CV191070 vs. TAIR peptide
Match: AT4G19130.1 (| Symbols: | Replication factor-A protein 1-related | chr4:10466618-10469092 REVERSE LENGTH=784)

HSP 1 Score: 90.8929 bits (224), Expect = 6.430e-19
Identity = 59/146 (40.41%), Postives = 86/146 (58.90%), Query Frame = 3

Query:    9 GAIAMLSSGDAQ-EADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEP----KHFDRPGSNTN---SAPAARSSSQVQSNVDQPG 422
            GAI  + +G+   EAD+ PV+QV DL+ +  Q Q+   ER+R++LSDG  L QG+L T  N+LV    LQ GSIV+L+ FV ++I GR  +IV  L V+    D+IG P    KH D+ G+++    +    + S   Q N  +PG
Sbjct:    8 GAIGKIMNGEVTTEADMIPVLQVTDLKQIMAQ-QDPTRERFRMVLSDGTYLHQGMLGTDLNNLVKEGTLQPGSIVRLTRFVGDVIKGRRIVIVPQLEVLKQISDIIGHPVPGGKHNDQRGADSGIKFNTTEQQGSGIRQVNNIEPG 152

[Close]

BLAST of CV191070 vs. TAIR peptide
Match: AT2G06510.1 (| Symbols: ATRPA1A, RPA1A, RPA70A, ATRPA70A | replication protein A 1A | chr2:2585215-2587601 FORWARD LENGTH=640)

HSP 1 Score: 85.8853 bits (211), Expect = 2.068e-17
Identity = 42/110 (38.18%), Postives = 73/110 (66.36%), Query Frame = 3

Query:   12 AIAMLSSGDAQEADLKPVVQVVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHF 341
            AI  +  GD    +LKP++QV++++++  ++Q +  ERYR L+SDGV+ Q  ++A Q ND V +   +KGSIVQL +++C+ + GR  I+V+++  I+ + + IG P  F
Sbjct:    9 AITAIHDGDV---NLKPLLQVLEIKMIG-RSQERSQERYRFLISDGVSAQHAMVAVQLNDRVKSGQFEKGSIVQLIDYICSDVKGRKLIVVLNMETIVQQSETIGNPTIF 114

[Close]

BLAST of CV191070 vs. TAIR peptide
Match: AT2G06510.2 (| Symbols: ATRPA1A, RPA1A, RPA70A, ATRPA70A | replication protein A 1A | chr2:2585215-2587601 FORWARD LENGTH=617)

HSP 1 Score: 74.3294 bits (181), Expect = 6.228e-14
Identity = 34/90 (37.78%), Postives = 61/90 (67.78%), Query Frame = 3

Query:   72 VVDLRLVNTQNQNQQNERYRVLLSDGVNLQQGILATQKNDLVHTQALQKGSIVQLSEFVCNLILGRTSIIVIDLNVILDKCDVIGEPKHF 341
            V++++++  ++Q +  ERYR L+SDGV+ Q  ++A Q ND V +   +KGSIVQL +++C+ + GR  I+V+++  I+ + + IG P  F
Sbjct:    3 VLEIKMIG-RSQERSQERYRFLISDGVSAQHAMVAVQLNDRVKSGQFEKGSIVQLIDYICSDVKGRKLIVVLNMETIVQQSETIGNPTIF 91

The following BLAST results are available for this feature:

BLAST of CV191070 vs. DB:swissprot:display
BLAST of CV191070 vs. Populus trichocarpa peptide v2.0
BLAST of CV191070 vs. P. persica 1.0 peptide
BLAST of CV191070 vs. TrEMBL
BLAST of CV191070 vs. Vitis vinifera peptide
BLAST of CV191070 vs. TAIR peptide

BLAST of CV191070 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
RFA1_SCHPO	7.688e-16	34.25	Replication factor A protein 1 OS=Schizosaccharomy...	[more]
RFA1_XENLA	2.315e-12	36.84	Replication protein A 70 kDa DNA-binding subunit O...	[more]
RFA1_PONAB	6.735e-12	34.64	Replication protein A 70 kDa DNA-binding subunit O...	[more]
RFA1_HUMAN	1.500e-11	34.48	Replication protein A 70 kDa DNA-binding subunit O...	[more]
RFA1_CHICK	4.365e-11	38.97	Replication protein A 70 kDa DNA-binding subunit O...	[more]
RFA1_XENTR	9.725e-11	39.71	Replication protein A 70 kDa DNA-binding subunit O...	[more]
RFA1_MOUSE	1.404e-9	35.65	Replication protein A 70 kDa DNA-binding subunit O...	[more]
RFA1_DROME	6.969e-9	29.33	Replication protein A 70 kDa DNA-binding subunit O...	[more]

BLAST of CV191070 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
POPTR_0003s10170.1	1.985e-24	42.36		[more]
POPTR_0018s07150.1	6.183e-18	38.46		[more]

BLAST of CV191070 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
ppa002611m	8.808e-19	38.33		[more]
ppa001396m	1.661e-17	52.33		[more]

BLAST of CV191070 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
A5AQN6\|A5AQN6_VITVI	1.766e-44	53.85	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7U3Y7\|D7U3Y7_VITVI	1.037e-36	64.96	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9TFZ6\|A9TFZ6_PHYPA	3.582e-21	38.26	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]
A9TA55\|A9TA55_PHYPA	3.582e-21	40.69	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]
Q65XV7\|Q65XV7_ORYSJ	3.961e-20	41.91	Os05g0111000 protein OS=Oryza sativa subsp. japoni...	[more]
B8AWZ3\|B8AWZ3_ORYSI	3.961e-20	41.91	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q9FHJ6\|Q9FHJ6_ARATH	5.719e-19	36.30	Replication protein A1-like OS=Arabidopsis thalian...	[more]
D7KGL1\|D7KGL1_ARALY	9.756e-19	41.86	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B6U4N8\|B6U4N8_MAIZE	2.838e-18	38.10	DNA binding protein OS=Zea mays PE=2 SV=1	[more]
B3VTC8\|B3VTC8_SORBI	4.842e-18	36.49	Putative uncharacterized protein Sb09g001260 OS=So...	[more]

BLAST of CV191070 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 2


Match Name	E-value	Identity	Description
GSVIVT01022784001	5.014e-39	64.96	assembled CDS	[more]
GSVIVT01015111001	1.517e-19	41.67	assembled CDS	[more]

BLAST of CV191070 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G45400.1	4.027e-21	36.30	\| Symbols: RPA70C, ATRPA70C \| Replication factor-A...	[more]
AT4G19130.1	6.430e-19	40.41	\| Symbols: \| Replication factor-A protein 1-relat...	[more]
AT2G06510.1	2.068e-17	38.18	\| Symbols: ATRPA1A, RPA1A, RPA70A, ATRPA70A \| repl...	[more]
AT2G06510.2	6.228e-14	37.78	\| Symbols: ATRPA1A, RPA1A, RPA70A, ATRPA70A \| repl...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 48..59 score
None	No IPR available	SEG	seg	seg	coord: 14..28 score
None	No IPR available	SEG	seg	seg	coord: 147..160 score
None	No IPR available	SEG	seg	seg	coord: 25..34 score
None	No IPR available	SEG	seg	seg	coord: 20..30 score
None	No IPR available	SEG	seg	seg	coord: 45..56 score
IPR007199	Replication factor-A protein 1, N-terminal	PFAM	PF04057	Rep-A_N	coord: 2..100 score: 1.4
IPR012340	Nucleic acid-binding, OB-fold	GENE3D	2.40.50.140	no description	coord: 2..112 score: 1
IPR016027	Nucleic acid-binding, OB-fold-like	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 2..109 score: 1.5

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV191070 ID=CV191070; Name=CV191070; organism=Vaccinium corymbosum; type=EST; length=650bp
AGGCGGAAGGGGCGATAGCAATGCTGTCGAGCGGCGACGCTCAGGAAGCG
GACTTGAAGCCGGTGGTCCAAGTGGTGGACTTGAGACTGGTCAACACCCA
GAACCAGAACCAGCAAAACGAGAGGTACCGTGTTCTTCTCTCCGACGGTG
TTAATCTCCAGCAGGGCATACTCGCCACTCAGAAGAACGATTTGGTCCAT
ACCCAGGCCCTTCAGAAGGGATCCATCGTTCAGTTGTCTGAGTTCGTCTG
TAATCTCATCCTAGGCCGCACGAGTATTATTGTCATCGACCTAAATGTGA
TACTTGACAAGTGTGATGTAATAGGTGAACCAAAACATTTTGATCGACCT
GGCAGTAATACTAATTCTGCTCCAGCAGCAAGATCCTCTTCGCAGGTGCA
GTCTAATGTGGATCAACCTGGGACAATGACCAGTAATGCGCCATCTTCTG
TTGGTAGTCCGGTGGGTGTGGATGGCTCAGTTGCTAAACCAAATGTTATT
GGGGGAGCTTTTCCAATCCAACCTAAAGTGGATCGCAGCCCAGATTCAAC
TTTGGCCTTTGTTGCGACTTTCTTCGTAGGTTTCATCGCTTGTATTATGG
TGGCACCATTTATTAGGTTCGGAATGAAGCAGGAGATGACATAATAATAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0006260	DNA replication

Vocabulary: **KEGG ORTHOLOGY**
Term	Definition
K07466	RFA1, RPA1, rpa; replication factor A1

Vocabulary: **KEGG PATHWAY**
Term	Definition
ko03030	DNA replication

Vocabulary: **INTERPRO**
Term	Definition
noIPR	unintegrated
IPR007199	Rep_factor-A_N
IPR012340	NA-bd_OB-fold
IPR016027	Nucleic acid-binding, OB-fold-like