DW043492

EST Overview
NameDW043492
Unique NameDW043492
TypeEST
OrganismVaccinium corymbosum (northern highbush blueberry)
Sequence length494
Libraries
Library NameType
LIBEST_018810 Subtracted cDNA library of Vaccinium corymbosumcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Vaccinium corymbosum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Vaccinium corymbosum unigene v12011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL2011-01-18
InterProScan analysis for Vaccinium corymbosum unigene v12011-01-18
Homology
[Close]
BLAST of DW043492 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0013s05870.1 ()

HSP 1 Score: 55.4546 bits (132), Expect = 2.058e-8
Identity = 33/106 (31.13%), Postives = 52/106 (49.06%), Query Frame = -3 
Query:  133 GCRAEDKPDQLQSECNPEQKKGG-------GLMDKVKDKILGGHGGS-ADQQQSECKPDQRKEGG------GIMDKVKDKIPGVHGGGA---DQQRGECKPDQRRG 399
            G + +++  + Q  CN ++ +GG       G  D++KDKI GG GG    + Q  C   + + G       G + ++KDKIPG  GGG     + +G C   + RG
Sbjct:   15 GGKKDERKGETQGGCNQQEHRGGAQGERKEGFADQMKDKIPGGGGGGHKGETQGGCNQQEHRGGAQGERKEGFVGQMKDKIPGGGGGGVGGKGETQGGCNQQEHRG 120
[Close]
BLAST of DW043492 vs. TrEMBL
Match: Q68YT3|Q68YT3_VACCO (14 kDa dehydrin OS=Vaccinium corymbosum GN=bbdhn6 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.523e-44
Identity = 83/84 (98.81%), Postives = 83/84 (98.81%), Query Frame = -3 
Query:  130 KPDQLQSECNPEQKKGGGLMDKVKDKILGGHGGSADQQQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRGD 381
            KPDQLQSECNPEQKKGGGLMDKVKDKI GGHGGSADQQQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRGD
Sbjct:   18 KPDQLQSECNPEQKKGGGLMDKVKDKIPGGHGGSADQQQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRGD 101
[Close]
BLAST of DW043492 vs. TrEMBL
Match: O22623|O22623_VACCO (Dehydrin 1 OS=Vaccinium corymbosum GN=bbdhn1 PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 2.523e-20
Identity = 58/104 (55.77%), Postives = 65/104 (62.50%), Query Frame = -3 
Query:  130 KPDQLQSECNPEQKK-GGGLMDKVKDKILGGHGGSA-------------------DQQQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRGD 381
            + DQ   E   +Q+K GGGLMDKVKDKI GG+GGSA                   DQQ+ E K DQRKEGGG+MDKVKD I G  GGGAD+ RGE K DQ RGD
Sbjct:  211 RQDQQHGEYKQDQRKEGGGLMDKVKDKIPGGNGGSAADQHQGVYGQDQQLGGYRQDQQRGEYKQDQRKEGGGLMDKVKDTIHGGAGGGADKHRGEYKQDQYRGD 314

HSP 2 Score: 83.9593 bits (206), Expect = 5.445e-15
Identity = 55/125 (44.00%), Postives = 61/125 (48.80%), Query Frame = -3 
Query:  136 QLQSECNPEQKKGGGLMDKVKDKILGGHGGSADQQQS---------------------------ECKPDQRKEGGGIMDKVKDKIPGVHGGGA-------------------DQQRGECKPDQRR 372
            QL      + K+GGGL+ KVKDKI GG GGSADQ Q                            E K DQRKEGGG+MDKVKDKIPG +GG A                   DQQRGE K DQR+
Sbjct:  154 QLGGYRQDQHKEGGGLIYKVKDKIHGGDGGSADQHQGIYGQDQQLGGYRQDQQLGGYRQDQQHGEYKQDQRKEGGGLMDKVKDKIPGGNGGSAADQHQGVYGQDQQLGGYRQDQQRGEYKQDQRK 278

HSP 3 Score: 76.6406 bits (187), Expect = 8.693e-13
Identity = 58/146 (39.73%), Postives = 70/146 (47.95%), Query Frame = -3 
Query:  133 GKPGSIQSFKHGSNV*TS---IGRIN*RGIALGCRAEDKP------DQLQSECNPEQKK-GGGLMDKVKDKILGGHGGSADQQQSECKPDQ------------------RKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRG 486
            G  G ++   HG  V T    +G ++  G   G    D+       DQ       +Q+K GGGLMDKVKDKI GG  GSADQ Q   K DQ                   KEGGG++ KVKDKI G  GG ADQ +G    DQ+ G
Sbjct:   54 GLMGELKDKIHGGGVGTDGGGVGSVDPHGHQGGKYRHDQQHSEYVQDQQPGGYRQDQRKEGGGLMDKVKDKIHGGGVGSADQHQDGYKQDQLHGGYKQDQQLGGYRQDQHKEGGGLIYKVKDKIHGGDGGSADQHQGIYGQDQQLG 199

HSP 4 Score: 71.633 bits (174), Expect = 2.797e-11
Identity = 50/113 (44.25%), Postives = 58/113 (51.33%), Query Frame = -3 
Query:  133 DKPDQLQSECNPEQKK-GGGLMDKVKDKILGG--------------HGGSA-----DQQQSEC---------KPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRG 384
            +KP Q Q E   +Q+K GGGLM ++KDKI GG              HG        DQQ SE          + DQRKEGGG+MDKVKDKI G   G ADQ +   K DQ  G
Sbjct:   35 NKPGQHQGESRQDQRKEGGGLMGELKDKIHGGGVGTDGGGVGSVDPHGHQGGKYRHDQQHSEYVQDQQPGGYRQDQRKEGGGLMDKVKDKIHGGGVGSADQHQDGYKQDQLHG 147
[Close]
BLAST of DW043492 vs. TrEMBL
Match: Q9M5W7|Q9M5W7_VACCO (Dehydrin (Fragment) OS=Vaccinium corymbosum PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.136e-19
Identity = 57/104 (54.81%), Postives = 64/104 (61.54%), Query Frame = -3 
Query:  130 KPDQLQSECNPEQKKGGG-LMDKVKDKILGGHGGSA-------------------DQQQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRGD 381
            + DQ   E   +Q+K GG LMDKVKDKI GG+GGSA                   DQQ+ E K DQRKEGGG+MDKVKD I G  GGGAD+ RGE K DQ RGD
Sbjct:  105 RQDQQHGEYKQDQRKEGGWLMDKVKDKIPGGNGGSAADQHQGVYGQDQQLGGYRQDQQRGEYKQDQRKEGGGLMDKVKDTIHGGAGGGADKHRGEYKQDQYRGD 208

HSP 2 Score: 80.4925 bits (197), Expect = 6.020e-14
Identity = 52/117 (44.44%), Postives = 58/117 (49.57%), Query Frame = -3 
Query:  136 EQKKGGGLMDKVKDKILGGHGGSADQQQS---------------------------ECKPDQRKEGGGIMDKVKDKIPGVHGGGA-------------------DQQRGECKPDQRR 348
            + K+GGGL+ KVKDKI GG GGSADQ Q                            E K DQRKEGG +MDKVKDKIPG +GG A                   DQQRGE K DQR+
Sbjct:   56 QHKEGGGLIYKVKDKIHGGDGGSADQHQGIYGQDQQLGGYRQDQQLGGYRQDQQHGEYKQDQRKEGGWLMDKVKDKIPGGNGGSAADQHQGVYGQDQQLGGYRQDQQRGEYKQDQRK 172

HSP 3 Score: 62.003 bits (149), Expect = 2.216e-8
Identity = 36/77 (46.75%), Postives = 47/77 (61.04%), Query Frame = -3 
Query:  133 EQKKGGGLMDKV-----KDKILGGHGGSADQQQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRG 348
            +Q +GG   D++     +D+ LGGHG   DQQ    + DQ KEGGG++ KVKDKI G  GG ADQ +G    DQ+ G
Sbjct:   19 DQHQGGYKQDQLHGGYRQDQQLGGHG--QDQQPGGYRQDQHKEGGGLIYKVKDKIHGGDGGSADQHQGIYGQDQQLG 93
[Close]
BLAST of DW043492 vs. TrEMBL
Match: Q9M5W5|Q9M5W5_VACCO (Dehydrin (Fragment) OS=Vaccinium corymbosum PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.136e-19
Identity = 57/104 (54.81%), Postives = 64/104 (61.54%), Query Frame = -3 
Query:  130 KPDQLQSECNPEQKKGGG-LMDKVKDKILGGHGGSA-------------------DQQQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRGD 381
            + DQ   E   +Q+K GG LMDKVKDKI GG+GGSA                   DQQ+ E K DQRKEGGG+MDKVKD I G  GGGAD+ RGE K DQ RGD
Sbjct:  217 RQDQQHGEYKQDQRKEGGWLMDKVKDKIPGGNGGSAADQHQGVYGQDQQLGGYRQDQQRGEYKQDQRKEGGGLMDKVKDTIHGGAGGGADKHRGEYKQDQYRGD 320

HSP 2 Score: 80.8777 bits (198), Expect = 4.610e-14
Identity = 56/140 (40.00%), Postives = 65/140 (46.43%), Query Frame = -3 
Query:  136 GCRAEDKPDQLQSECNP------EQKKGGGLMDKVKDKILGGHGGSADQQQS---------------------------ECKPDQRKEGGGIMDKVKDKIPGVHGGGA-------------------DQQRGECKPDQRR 399
            G R + +P     +  P      + K+GGGL+ KVKDKI GG GGSADQ Q                            E K DQRKEGG +MDKVKDKIPG +GG A                   DQQRGE K DQR+
Sbjct:  145 GYRQDQQPGGYGQDQQPGGYRQDQHKEGGGLIYKVKDKIHGGDGGSADQHQGIYGQDQQLGGYRQDQQLGGYRQDQQHGEYKQDQRKEGGWLMDKVKDKIPGGNGGSAADQHQGVYGQDQQLGGYRQDQQRGEYKQDQRK 284

HSP 3 Score: 64.3142 bits (155), Expect = 4.465e-9
Identity = 46/118 (38.98%), Postives = 54/118 (45.76%), Query Frame = -3 
Query:  133 KPDQLQSECNPEQKKGG--------GLMDKVKDKILGGHGGSADQQQSECKPDQ---------------------------RKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRG 381
            + DQ       +Q++GG        GL DKVKDKI GG  GSADQ Q   K DQ                            KEGGG++ KVKDKI G  GG ADQ +G    DQ+ G
Sbjct:   88 RQDQQLGGHGQDQQRGGYRQDQHNEGLADKVKDKIHGGGVGSADQHQGGYKQDQLLGGYRQDQQPGGYGQDQQPGGYRQDQHKEGGGLIYKVKDKIHGGDGGSADQHQGIYGQDQQLG 205

HSP 4 Score: 56.9954 bits (136), Expect = 7.128e-7
Identity = 44/116 (37.93%), Postives = 52/116 (44.83%), Query Frame = -3 
Query:  133 KPDQLQSECNPEQKKGG--------GLMDKVKDKILGGHGGSADQQQSECKPDQ-----------------RKEGG--------GIMDKVKDKIPGVHGGGADQQRGECKPDQRRG 381
            + DQ       +Q++GG        GL DKVKDKI GG  GS DQ Q   K DQ                 ++ GG        G+ DKVKDKI G   G ADQ +G  K DQ  G
Sbjct:   29 RQDQQLGGHEQDQQRGGYRQDQHNEGLADKVKDKIHGGGVGSVDQHQGGYKQDQLHGGYRQDQQLGGHGQDQQRGGYRQDQHNEGLADKVKDKIHGGGVGSADQHQGGYKQDQLLG 144
[Close]
BLAST of DW043492 vs. TrEMBL
Match: Q9M5W8|Q9M5W8_VACCO (Dehydrin (Fragment) OS=Vaccinium corymbosum PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.029e-18
Identity = 57/111 (51.35%), Postives = 63/111 (56.76%), Query Frame = -3 
Query:  130 KPDQLQSECNPEQKKGG--------GLMDKVKDKILGGHGGSA-------------------DQQQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRGD 381
            K DQL      +Q+ GG        GL DKVKDKI GG+GGSA                   DQQ+ E K DQRKEGGG+MDKVKD I G  GGGAD+ RGE K DQ RGD
Sbjct:   21 KQDQLHGGYKQDQQLGGHRQDQHNEGLADKVKDKIPGGNGGSAADQHQGVYGQDQQLGGYRQDQQRGEYKQDQRKEGGGLMDKVKDTIHGGAGGGADKHRGEYKQDQYRGD 131
[Close]
BLAST of DW043492 vs. TrEMBL
Match: Q9M5W6|Q9M5W6_VACCO (Dehydrin (Fragment) OS=Vaccinium corymbosum PE=2 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 5.262e-18
Identity = 52/91 (57.14%), Postives = 58/91 (63.74%), Query Frame = -3 
Query:  130 EQKKGGGLMDKVKDKILGGHGGSADQ------------------QQSECKPDQRKEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRGD 348
            + K+GGGL+ KVKDKI GG GGSADQ                  Q+ E K DQRKEGGG+MDKVKD I G  GGGAD+ RGE K DQ RGD
Sbjct:  128 QHKEGGGLIYKVKDKIHGGDGGSADQHQGIYGQDQQLGGYRQDQQRGEYKQDQRKEGGGLMDKVKDTIHGGAGGGADKHRGEYKQDQYRGD 218

HSP 2 Score: 68.1662 bits (165), Expect = 3.092e-10
Identity = 46/109 (42.20%), Postives = 55/109 (50.46%), Query Frame = -3 
Query:  133 KPDQLQSECNPEQKKGG--------GLMDKVKDKILGGHGGSADQQQSECKPDQR------------------KEGGGIMDKVKDKIPGVHGGGADQQRGECKPDQRRG 381
            + DQ       +Q++GG        GL DKVKDKI GG  GSADQ Q   K DQ+                  KEGGG++ KVKDKI G  GG ADQ +G    DQ+ G
Sbjct:   57 RQDQQLGGHGQDQQRGGYRQDQHNEGLADKVKDKIHGGGVGSADQHQGGYKQDQQPGGYGQDQQPGGYRQDQHKEGGGLIYKVKDKIHGGDGGSADQHQGIYGQDQQLG 165

HSP 3 Score: 57.7658 bits (138), Expect = 4.179e-7
Identity = 40/91 (43.96%), Postives = 45/91 (49.45%), Query Frame = -3 
Query:  133 GLMDKVKDKILGGHGGSADQQQSECKPDQ-----------------RKEGG--------GIMDKVKDKIPGVHGGGADQQRGECKPDQRRG 330
            GL DKVKDKI GG  GSADQ Q   K DQ                 ++ GG        G+ DKVKDKI G   G ADQ +G  K DQ+ G
Sbjct:   23 GLADKVKDKIHGGGVGSADQHQGGYKQDQLHGGYRQDQQLGGHGQDQQRGGYRQDQHNEGLADKVKDKIHGGGVGSADQHQGGYKQDQQPG 113
[Close]
BLAST of DW043492 vs. TrEMBL
Match: Q9AR75|Q9AR75_PISVE (Dehydrin OS=Pistacia vera GN=COR PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.447e-8
Identity = 31/83 (37.35%), Postives = 47/83 (56.63%), Query Frame = -3 
Query:  145 KPDQLQSECNPEQ-KKGGGLMDKVKDKILGGHGGSADQQQSECKPDQRKEGGGIMDKVKDKIPG---VHGGGADQQRGECKPD 381
            K +Q    C PE  +   G++ K+K+K+  G G    QQ   CKP+  +   G+++K+KDK+PG    HG G  Q RGE +P+
Sbjct:   33 KVEQHHGVCKPEHGEHKEGMVQKIKNKV-PGQGDGVKQQHGVCKPEHGEHKEGLVEKIKDKVPGQGDQHGHGMQQHRGEYRPE 114

HSP 2 Score: 56.9954 bits (136), Expect = 7.128e-7
Identity = 31/87 (35.63%), Postives = 47/87 (54.02%), Query Frame = -3 
Query:  145 DKPDQLQSECNPEQ-KKGGGLMDKVKDKILGG---HGGSADQQQSECKPDQRKEGGGIMDKVKDKIPG---VHGGGADQQRGECKPD 384
            D   Q    C PE  +   GL++K+KDK+ G    HG    Q + E +P+  +   G+++K+KDK+PG    HG G  Q  GE +P+
Sbjct:   65 DGVKQQHGVCKPEHGEHKEGLVEKIKDKVPGQGDQHGHGMQQHRGEYRPEYGEHKEGLVEKIKDKLPGQGDQHGQGVQQHHGEYRPE 151
The following BLAST results are available for this feature:
BLAST of DW043492 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
POPTR_0013s05870.12.058e-831.13[more]
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BLAST of DW043492 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 7
Match NameE-valueIdentityDescription
Q68YT3|Q68YT3_VACCO9.523e-4498.8114 kDa dehydrin OS=Vaccinium corymbosum GN=bbdhn6 ... [more]
O22623|O22623_VACCO2.523e-2055.77Dehydrin 1 OS=Vaccinium corymbosum GN=bbdhn1 PE=2 ... [more]
Q9M5W7|Q9M5W7_VACCO2.136e-1954.81Dehydrin (Fragment) OS=Vaccinium corymbosum PE=2 S... [more]
Q9M5W5|Q9M5W5_VACCO2.136e-1954.81Dehydrin (Fragment) OS=Vaccinium corymbosum PE=2 S... [more]
Q9M5W8|Q9M5W8_VACCO4.029e-1851.35Dehydrin (Fragment) OS=Vaccinium corymbosum PE=2 S... [more]
Q9M5W6|Q9M5W6_VACCO5.262e-1857.14Dehydrin (Fragment) OS=Vaccinium corymbosum PE=2 S... [more]
Q9AR75|Q9AR75_PISVE6.447e-837.35Dehydrin OS=Pistacia vera GN=COR PE=2 SV=1[more]
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InterPro
Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSEGsegsegcoord: 3..18
score: NAcoord: 40..52
score: NAcoord: 74..84
score
IPR000167DehydrinPFAMPF00257Dehydrincoord: 23..59
score: 1.3e-05coord: 58..93
score: 1.8

Properties
Property NameValue
Singlet in analysisVaccinium corymbosum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>DW043492 ID=DW043492; Name=DW043492; organism=Vaccinium corymbosum; type=EST; length=494bp
GTTACAGAATAAGAAGAAAAACAGATACCTCGGTTGTGTGCATGTCTATA
TATACTACAGGTCTGTGTAGTTACCTAAAGAGTAGCTAGCGTAGAGGCGG
AAAAGAAGACGCGATGATGATTCGATCTAATCGCCGCGGCGCTGATCCGG
CTTGCACTCGCCGCGCTGTTGATCAGCACCACCACCATGGACGCCAGGGA
TCTTATCCTTCACCTTGTCCATAATCCCTCCACCTTCCTTGCGCTGATCC
GGCTTGCACTCGCTTTGCTGCTGATCAGCACTACCACCATGGCCGCCAAG
GATCTTATCCTTCACCTTGTCCATAAGCCCTCCACCTTTCTTCTGCTCCG
GGTTGCACTCGCTTTGCAGCTGATCTGGTTTGTCCTCCGCGCGACACCCT
AAGGCAATTCCGCGCTAATTAATTCGCCCTATACTAGTTTATACATTACT
GCCGTGTTTAAAACTTTGAATGGAACCCGGCTTCCCCTATTCCT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Biological Process
TermDefinition
GO:0006950response to stress
GO:0009415response to water
Vocabulary: INTERPRO
TermDefinition
noIPRunintegrated
IPR000167Dehydrin