CF810543 | GDV

EST Overview


Name	CF810543
Unique Name	CF810543
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	576

Libraries


Library Name	Type
LIBEST_014530 cDNA cold acclimated Bluecrop library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of CF810543 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0016s07520.1 ()

HSP 1 Score: 91.6633 bits (226), Expect = 3.431e-19
Identity = 45/58 (77.59%), Postives = 51/58 (87.93%), Query Frame = 3

Query:  393 VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            +ETRSR QTPS S VG  PRLKD+SNALTTSKELLKIINR+W  +D+PSSS+SLISAL
Sbjct:  185 IETRSRAQTPSGSTVGVRPRLKDVSNALTTSKELLKIINRVWGNEDRPSSSMSLISAL 242

[Close]

BLAST of CF810543 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0006s22430.1 ()

HSP 1 Score: 90.8929 bits (224), Expect = 5.852e-19
Identity = 45/58 (77.59%), Postives = 51/58 (87.93%), Query Frame = 3

Query:  393 VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            +ETRS+ QTPS S VG  PRLKD+SNALTTSKELLKIINR+W  +D+PSSSLSLISAL
Sbjct:  194 IETRSQAQTPSGSTVGVKPRLKDVSNALTTSKELLKIINRVWGNEDRPSSSLSLISAL 251

[Close]

BLAST of CF810543 vs. P. persica 1.0 peptide
Match: ppa002441m ()

HSP 1 Score: 85.5001 bits (210), Expect = 9.628e-19
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 3

Query:  393 VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            VETRSR QTP+ S VG    LKD+SNALTTSKELLKIINRIW  +D+PSSS++LISAL
Sbjct:  198 VETRSRAQTPTASTVGVKTHLKDVSNALTTSKELLKIINRIWGNEDRPSSSMALISAL 255

HSP 2 Score: 24.6386 bits (52), Expect = 9.628e-19
Identity = 10/19 (52.63%), Postives = 14/19 (73.68%), Query Frame = 1

Query:  226 LAEHNVGLLDSLSSASLME 282
            L +H+ G+LDS  + SLME
Sbjct:  179 LTDHHAGMLDSHRNGSLME 197

[Close]

BLAST of CF810543 vs. TrEMBL
Match: B9HBW4|B9HBW4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_561459 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 6.730e-17
Identity = 45/58 (77.59%), Postives = 51/58 (87.93%), Query Frame = 3

Query:  393 VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            +ETRS+ QTPS S VG  PRLKD+SNALTTSKELLKIINR+W  +D+PSSSLSLISAL
Sbjct:  194 IETRSQAQTPSGSTVGVKPRLKDVSNALTTSKELLKIINRVWGNEDRPSSSLSLISAL 251

[Close]

BLAST of CF810543 vs. TrEMBL
Match: B9SX00|B9SX00_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1258630 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 7.441e-16
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 3

Query:  393 VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            +ET SR QTPS S VG   RLKD+SNALTTSKELLKIINR+W  +D+PSSS+SLISAL
Sbjct:  210 IETSSRAQTPSGSTVGAKTRLKDVSNALTTSKELLKIINRVWGNEDRPSSSMSLISAL 267

[Close]

BLAST of CF810543 vs. TrEMBL
Match: Q9CAW9|Q9CAW9_ARATH (At3g11590/F24K9_26 OS=Arabidopsis thaliana GN=At3g11590 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.828e-15
Identity = 43/61 (70.49%), Postives = 51/61 (83.61%), Query Frame = 3

Query:  384 FA*VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            F  +ETRSR +TP+ S VG   RLKD SNALTTSKELLKIINR+W +DD+PSSS+SL+SAL
Sbjct:  200 FMDIETRSRVETPTGSTVGVKTRLKDCSNALTTSKELLKIINRMWGQDDRPSSSMSLVSAL 260

[Close]

BLAST of CF810543 vs. TrEMBL
Match: Q945L6|Q945L6_ARATH (AT3g11590/F24K9_26 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.828e-15
Identity = 43/61 (70.49%), Postives = 51/61 (83.61%), Query Frame = 3

Query:  384 FA*VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            F  +ETRSR +TP+ S VG   RLKD SNALTTSKELLKIINR+W +DD+PSSS+SL+SAL
Sbjct:  200 FMDIETRSRVETPTGSTVGVKTRLKDCSNALTTSKELLKIINRMWGQDDRPSSSMSLVSAL 260

[Close]

BLAST of CF810543 vs. TrEMBL
Match: D7LAP5|D7LAP5_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_478504 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.828e-15
Identity = 43/61 (70.49%), Postives = 51/61 (83.61%), Query Frame = 3

Query:  384 FA*VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            F  +ETRSR +TP+ S VG   RLKD SNALTTSKELLKIINR+W +DD+PSSS+SL+SAL
Sbjct:  199 FMDIETRSRVETPTGSTVGVKTRLKDCSNALTTSKELLKIINRMWGQDDRPSSSMSLVSAL 259

[Close]

BLAST of CF810543 vs. TrEMBL
Match: D7TH30|D7TH30_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00033905001 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.693e-15
Identity = 44/58 (75.86%), Postives = 49/58 (84.48%), Query Frame = 3

Query:  393 VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            +E RSR QTPS S VG   RLKD+SNALTTSKELLKII+RIW  +DQPSSS+SLISAL
Sbjct:  187 IEIRSRGQTPSGSTVGVRTRLKDVSNALTTSKELLKIISRIWGHEDQPSSSMSLISAL 244

[Close]

BLAST of CF810543 vs. TrEMBL
Match: A5BVN9|A5BVN9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_017038 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.693e-15
Identity = 44/58 (75.86%), Postives = 49/58 (84.48%), Query Frame = 3

Query:  393 VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            +E RSR QTPS S VG   RLKD+SNALTTSKELLKII+RIW  +DQPSSS+SLISAL
Sbjct:  187 IEIRSRGQTPSGSTVGVRTRLKDVSNALTTSKELLKIISRIWGHEDQPSSSMSLISAL 244

[Close]

BLAST of CF810543 vs. TrEMBL
Match: D7TCQ8|D7TCQ8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_80.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036076001 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.814e-11
Identity = 33/57 (57.89%), Postives = 44/57 (77.19%), Query Frame = 3

Query:  396 ETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            ET+SR +T S  IVG   RLKD+SN L TSKELLK++NRIW  +++ SSS+ ++SAL
Sbjct:  178 ETQSRGRTHSGCIVGVKTRLKDVSNGLATSKELLKVLNRIWGLEEKNSSSIPVVSAL 234

HSP 2 Score: 25.0238 bits (53), Expect = 5.814e-11
Identity = 16/42 (38.10%), Postives = 18/42 (42.86%), Query Frame = 1

Query:  157 ERMDXXXXXXXXXXXXXXXXXXXLAEHNVGLLDSLSSASLME 282
            ERMD                     E N+G LDSLS+AS ME
Sbjct:  131 ERMDRSRGGSHRRRRSAVSQKLQTTECNLGGLDSLSTASFME 172

[Close]

BLAST of CF810543 vs. TrEMBL
Match: A5BS09|A5BS09_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_019055 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.129e-10
Identity = 33/57 (57.89%), Postives = 44/57 (77.19%), Query Frame = 3

Query:  396 ETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            ET+SR +T S  IVG   RLKD+SN L TSKELLK++NRIW  +++ SSS+ ++SAL
Sbjct:  178 ETQSRGRTHSGCIVGVKTRLKDVSNGLATSKELLKVLNRIWGLEEKNSSSIPVVSAL 234

HSP 2 Score: 23.0978 bits (48), Expect = 2.129e-10
Identity = 16/42 (38.10%), Postives = 22/42 (52.38%), Query Frame = 2

Query:  158 RGWIDRVQEVATGGHHQFLIRSGLQ-STMLAFWIPLAVPV*W 280
            R WID +   ATGG  +  +RS  + S +   W   A+PV W
Sbjct:  106 REWID-LGVAATGGEGRRFLRSFRRLSVIWEGWTLSALPVSW 146

[Close]

BLAST of CF810543 vs. TrEMBL
Match: B9SCY4|B9SCY4_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1282280 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.963e-8
Identity = 31/61 (50.82%), Postives = 44/61 (72.13%), Query Frame = 3

Query:  384 FA*VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            F  +ET ++ +   E  +G   RLKD+SN LT SKELLK++NRIW  D+Q SS+++L+SAL
Sbjct:  170 FMEMETHTKGKNRRECNLGIRTRLKDVSNGLTASKELLKVLNRIWGLDEQHSSTMALVSAL 230

[Close]

BLAST of CF810543 vs. Vitis vinifera peptide
Match: GSVIVT01033905001 (assembled CDS)

HSP 1 Score: 85.1149 bits (209), Expect = 1.979e-17
Identity = 44/58 (75.86%), Postives = 49/58 (84.48%), Query Frame = 3

Query:  393 VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            +E RSR QTPS S VG   RLKD+SNALTTSKELLKII+RIW  +DQPSSS+SLISAL
Sbjct:  187 IEIRSRGQTPSGSTVGVRTRLKDVSNALTTSKELLKIISRIWGHEDQPSSSMSLISAL 244

[Close]

BLAST of CF810543 vs. Vitis vinifera peptide
Match: GSVIVT01036076001 (assembled CDS)

HSP 1 Score: 66.2402 bits (160), Expect = 3.076e-13
Identity = 33/57 (57.89%), Postives = 44/57 (77.19%), Query Frame = 3

Query:  396 ETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            ET+SR +T S  IVG   RLKD+SN L TSKELLK++NRIW  +++ SSS+ ++SAL
Sbjct:  178 ETQSRGRTHSGCIVGVKTRLKDVSNGLATSKELLKVLNRIWGLEEKNSSSIPVVSAL 234

HSP 2 Score: 25.0238 bits (53), Expect = 3.076e-13
Identity = 16/42 (38.10%), Postives = 18/42 (42.86%), Query Frame = 1

Query:  157 ERMDXXXXXXXXXXXXXXXXXXXLAEHNVGLLDSLSSASLME 282
            ERMD                     E N+G LDSLS+AS ME
Sbjct:  131 ERMDRSRGGSHRRRRSAVSQKLQTTECNLGGLDSLSTASFME 172

[Close]

BLAST of CF810543 vs. TAIR peptide
Match: AT3G11590.1 (| Symbols: | unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G22310.1); Has 22320 Blast hits to 15179 proteins in 1213 species: Archae - 372; Bacteria - 2307; Metazoa - 10906; Fungi - 1700; Plants - 1146; Viruses - 65; Other Eukaryotes - 5824 (source: NCBI BLink). | chr3:3660628-3663537 FORWARD LENGTH=622)

HSP 1 Score: 85.5001 bits (210), Expect = 2.168e-17
Identity = 43/61 (70.49%), Postives = 51/61 (83.61%), Query Frame = 3

Query:  384 FA*VETRSRCQTPSESIVGGGPRLKDISNALTTSKELLKIINRIWSRDDQPSSSLSLISAL 566
            F  +ETRSR +TP+ S VG   RLKD SNALTTSKELLKIINR+W +DD+PSSS+SL+SAL
Sbjct:  200 FMDIETRSRVETPTGSTVGVKTRLKDCSNALTTSKELLKIINRMWGQDDRPSSSMSLVSAL 260

The following BLAST results are available for this feature:

BLAST of CF810543 vs. Populus trichocarpa peptide v2.0
BLAST of CF810543 vs. P. persica 1.0 peptide
BLAST of CF810543 vs. TrEMBL
BLAST of CF810543 vs. Vitis vinifera peptide
BLAST of CF810543 vs. TAIR peptide

BLAST of CF810543 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
POPTR_0016s07520.1	3.431e-19	77.59		[more]
POPTR_0006s22430.1	5.852e-19	77.59		[more]

BLAST of CF810543 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ppa002441m	9.628e-19	74.14		[more]

BLAST of CF810543 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
B9HBW4\|B9HBW4_POPTR	6.730e-17	77.59	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SX00\|B9SX00_RICCO	7.441e-16	74.14	Putative uncharacterized protein OS=Ricinus commun...	[more]
Q9CAW9\|Q9CAW9_ARATH	2.828e-15	70.49	At3g11590/F24K9_26 OS=Arabidopsis thaliana GN=At3g...	[more]
Q945L6\|Q945L6_ARATH	2.828e-15	70.49	AT3g11590/F24K9_26 OS=Arabidopsis thaliana PE=2 SV...	[more]
D7LAP5\|D7LAP5_ARALY	2.828e-15	70.49	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D7TH30\|D7TH30_VITVI	3.693e-15	75.86	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BVN9\|A5BVN9_VITVI	3.693e-15	75.86	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7TCQ8\|D7TCQ8_VITVI	5.814e-11	57.89	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5BS09\|A5BS09_VITVI	2.129e-10	57.89	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9SCY4\|B9SCY4_RICCO	1.963e-8	50.82	Putative uncharacterized protein OS=Ricinus commun...	[more]

BLAST of CF810543 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 2


Match Name	E-value	Identity	Description
GSVIVT01033905001	1.979e-17	75.86	assembled CDS	[more]
GSVIVT01036076001	3.076e-13	57.89	assembled CDS	[more]

BLAST of CF810543 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G11590.1	2.168e-17	70.49	\| Symbols: \| unknown protein; LOCATED IN: plasma ...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 2..30 score: NAcoord: 35..52 score: NAcoord: 57..75 score: NAcoord: 123..135 score
None	No IPR available	SEG	seg	seg	coord: 39..66 score
None	No IPR available	SEG	seg	seg	coord: 44..68 score

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF810543 ID=CF810543; Name=CF810543; organism=Vaccinium corymbosum; type=EST; length=576bp
GTTAGAGAGGGTTTCGAGGAGGAGGAGAAGAAGAAGAAGAAGAAAAAGAA
GAGAGAGATGATGATGATGAGGGTTGGAGAGAGAATGGGGGCCAGGTCGG
TGCATTCGGTTTCCCTTCCTCCGCATTTGTCTGATCCGTCTCACAGTCCC
GTTTCTGAGAGGATGGATCGATCGGGTACAGGAAGTCGCCACCGGAGGAC
ATCATCAATTTCTCATAAGATCAGGCTTGCAGAGCACAATGTTGGCCTTT
TGGATTCCCTTAGCAGTGCCAGTTTAATGGAGCATCAGGATTCCAATAAG
CAGATTGGGGCAGGCCATAGAGTTAGTGATCTTTATATTTTGGAGATTTT
GCAAATCCGTCCGAGTCTCCTAAAATATGGAGATTTGCTTAAGTTGAGAC
TAGATCCCGATGTCAGACTCCTAGCGAGTCCATAGTTGGAGGTGGGCCAC
GTTTGAAGGATATTAGTAATGCTTTGACAACATCTAAAGAGCTACTGAAA
ATAATCAACCGTATCTGGTCTCGTGATGATCAGCCTTCATCAAGCTTGTC
CCTAATCTCTGCCCTAACATGCGGAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
noIPR	unintegrated