DW043271

EST Overview
NameDW043271
Unique NameDW043271
TypeEST
OrganismVaccinium corymbosum (northern highbush blueberry)
Sequence length519
Libraries
Library NameType
LIBEST_018810 Subtracted cDNA library of Vaccinium corymbosumcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Vaccinium corymbosum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Vaccinium corymbosum unigene v12011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL2011-01-18
Homology
[Close]
BLAST of DW043271 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0003s08360.1 ()

HSP 1 Score: 66.6254 bits (161), Expect = 1.624e-16
Identity = 52/133 (39.10%), Postives = 67/133 (50.38%), Query Frame = -2 
Query:   87 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQLLQE-------KNLEFWKTGGTISG*KTSRANGSSRVMNDSR*RCLCSVMHATETRYLWGGT*TQSVRADRDASLMALEECG 464
            M +TLRS TSFI+L+ TRS K  D  S  VCFAQ++PSCRL+AK+  Q  QL Q+       +  + W+    ISG   S +N    V           VM   +T  + G       RA  +ASLMAL   G
Sbjct:    1 MTITLRSSTSFISLRHTRSLKTPDGFSGTVCFAQIKPSCRLQAKNSKQEAQLSQDDILVTEGRKSKNWEKLHAISGPDHSSSNSRVPVF----------VMLPLDTVTIGGN--LNKPRA-MNASLMALRSAG 120

HSP 2 Score: 36.1946 bits (82), Expect = 1.624e-16
Identity = 16/26 (61.54%), Postives = 19/26 (73.08%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            +SAG+      DAWWGLVEK+GP KY
Sbjct:  117 RSAGVE-GVMVDAWWGLVEKDGPLKY 141
[Close]
BLAST of DW043271 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0003s03330.1 ()

HSP 1 Score: 64.6994 bits (156), Expect = 2.598e-16
Identity = 37/85 (43.53%), Postives = 51/85 (60.00%), Query Frame = -2 
Query:  222 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQLLQEKNL----EFWKTGGTISG*KTSRANGSSRV 464
            M   L+S TSFI+LKDTRS K  D+ S  +CFA ++PSCRL+AK+ MQ  QL  ++ L       K GG +      R++  S+V
Sbjct:    1 MTSALQSSTSFISLKDTRSPKTPDDFSGTICFAHIKPSCRLQAKNSMQEAQLSHDEILMTEGRKSKKGGELHAISGPRSSNDSKV 85

HSP 2 Score: 36.1946 bits (82), Expect = 2.598e-16
Identity = 16/26 (61.54%), Postives = 19/26 (73.08%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            +SAG+      DAWWGLVEK+GP KY
Sbjct:  116 RSAGVE-GVMVDAWWGLVEKDGPLKY 140

HSP 3 Score: 20.7866 bits (42), Expect = 2.598e-16
Identity = 7/13 (53.85%), Postives = 11/13 (84.62%), Query Frame = -1 
Query:  133 DTVSLGGHLNTVR 171
            DT+++GG+LN  R
Sbjct:   94 DTITIGGNLNKPR 106
[Close]
BLAST of DW043271 vs. P. persica 1.0 peptide
Match: ppa003812m ()

HSP 1 Score: 80.1073 bits (196), Expect = 2.681e-22
Identity = 41/65 (63.08%), Postives = 48/65 (73.85%), Query Frame = -2 
Query:  270 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQLLQEKNLEFWKTGG 464
            M LTLRS TSFINL D +S KA DE S  +C+AQ++P+CRL+AKS MQ TQLLQEK L    T G
Sbjct:    1 MTLTLRSSTSFINLNDHKSLKASDESSGTICYAQIKPTCRLRAKSSMQETQLLQEKTLMTAGTDG 65

HSP 2 Score: 38.5058 bits (88), Expect = 2.681e-22
Identity = 17/26 (65.38%), Postives = 20/26 (76.92%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            K+AG+      DAWWGLVEK+GPSKY
Sbjct:  116 KNAGVE-GVMVDAWWGLVEKDGPSKY 140

HSP 3 Score: 23.0978 bits (48), Expect = 2.681e-22
Identity = 10/13 (76.92%), Postives = 10/13 (76.92%), Query Frame = -1 
Query:  133 DTVSLGGHLNTVR 171
            DTVS GGHLN  R
Sbjct:   94 DTVSHGGHLNKPR 106
[Close]
BLAST of DW043271 vs. TrEMBL
Match: B9RUR8|B9RUR8_RICCO (Beta-amylase, putative OS=Ricinus communis GN=RCOM_0856140 PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.098e-17
Identity = 54/126 (42.86%), Postives = 70/126 (55.56%), Query Frame = -2 
Query:   87 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQLLQEKNLEFWKTGGTISG*KTSRANGSSRVMNDSR*RCLCSVMHATETRYLWGGT*TQSVRADRDASLMALEECG 464
            M+LTL S TSFIN+KDT+S K  D+ S  +CFAQ++PSCRL AK+ MQ  QL Q+    F   G      +   A  +S+  NDS  +    VM   +T  L G       RA  +ASLMAL+  G
Sbjct:    1 MSLTLHSSTSFINIKDTKSVKTPDDFSGTICFAQIKPSCRLGAKNSMQEAQLSQDN--IFTMEGRRSDNREKLHAMSNSQSSNDS--KVPVFVMLPLDTVTLGGN--LNKPRA-MNASLMALKSAG 119

HSP 2 Score: 37.7354 bits (86), Expect = 3.098e-17
Identity = 17/26 (65.38%), Postives = 19/26 (73.08%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            KSAG+      DAWWGLVEK+GP KY
Sbjct:  116 KSAGVE-GVMVDAWWGLVEKDGPRKY 140
[Close]
BLAST of DW043271 vs. TrEMBL
Match: D0VBH4|D0VBH4_EUPES (Beta-amylase 1 OS=Euphorbia esula GN=BAM1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 5.932e-15
Identity = 34/52 (65.38%), Postives = 40/52 (76.92%), Query Frame = -2 
Query:  309 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQL 464
            MALTLRS TSFINLKD++S KA D +SS + FAQM PSC L+ K+  QG QL
Sbjct:    1 MALTLRSSTSFINLKDSKSLKAPDGISSTISFAQMMPSCSLRVKNSTQGAQL 52

HSP 2 Score: 32.7278 bits (73), Expect = 5.932e-15
Identity = 15/26 (57.69%), Postives = 17/26 (65.38%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            KSAG+      D WWGLVEK+GP  Y
Sbjct:  116 KSAGVE-GVMVDVWWGLVEKDGPLIY 140

HSP 3 Score: 22.3274 bits (46), Expect = 5.932e-15
Identity = 18/51 (35.29%), Postives = 24/51 (47.06%), Query Frame = -1 
Query:  133 LEDRRNDKWVKDFTGERKLTGNERFQVTLPVFCDACH*DTVSLGGHLNTVR 285
            LE  +++KW K        T N      +PVF      DT++LGG LN  R
Sbjct:   60 LEGNKSNKWEKVSEISIPQTSNGP---KVPVFV-MLPLDTITLGGKLNRPR 106
[Close]
BLAST of DW043271 vs. TrEMBL
Match: A9PGR1|A9PGR1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_580143 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.676e-14
Identity = 52/133 (39.10%), Postives = 67/133 (50.38%), Query Frame = -2 
Query:   87 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQLLQE-------KNLEFWKTGGTISG*KTSRANGSSRVMNDSR*RCLCSVMHATETRYLWGGT*TQSVRADRDASLMALEECG 464
            M +TLRS TSFI+L+ TRS K  D  S  VCFAQ++PSCRL+AK+  Q  QL Q+       +  + W+    ISG   S +N    V           VM   +T  + G       RA  +ASLMAL   G
Sbjct:    1 MTITLRSSTSFISLRHTRSLKTPDGFSGTVCFAQIKPSCRLQAKNSKQEAQLSQDDILVTEGRKSKNWEKLHAISGPDHSSSNSRVPVF----------VMLPLDTVTIGGN--LNKPRA-MNASLMALRSAG 120

HSP 2 Score: 36.1946 bits (82), Expect = 1.676e-14
Identity = 16/26 (61.54%), Postives = 19/26 (73.08%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            +SAG+      DAWWGLVEK+GP KY
Sbjct:  117 RSAGVE-GVMVDAWWGLVEKDGPLKY 141
[Close]
BLAST of DW043271 vs. TrEMBL
Match: B9MTV8|B9MTV8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_589941 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.709e-14
Identity = 37/85 (43.53%), Postives = 51/85 (60.00%), Query Frame = -2 
Query:  222 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQLLQEKNL----EFWKTGGTISG*KTSRANGSSRV 464
            M   L+S TSFI+LKDTRS K  D+ S  +CFA ++PSCRL+AK+ MQ  QL  ++ L       K GG +      R++  S+V
Sbjct:    1 MTSALQSSTSFISLKDTRSPKTPDDFSGTICFAHIKPSCRLQAKNSMQEAQLSHDEILMTEGRKSKKGGELHAISGPRSSNDSKV 85

HSP 2 Score: 36.1946 bits (82), Expect = 2.709e-14
Identity = 16/26 (61.54%), Postives = 19/26 (73.08%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            +SAG+      DAWWGLVEK+GP KY
Sbjct:  116 RSAGVE-GVMVDAWWGLVEKDGPLKY 140

HSP 3 Score: 20.7866 bits (42), Expect = 2.709e-14
Identity = 7/13 (53.85%), Postives = 11/13 (84.62%), Query Frame = -1 
Query:  133 DTVSLGGHLNTVR 171
            DT+++GG+LN  R
Sbjct:   94 DTITIGGNLNKPR 106
[Close]
BLAST of DW043271 vs. TrEMBL
Match: D0VBH5|D0VBH5_EUPES (Beta-amylase 2 OS=Euphorbia esula GN=BAM2 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.209e-13
Identity = 33/52 (63.46%), Postives = 39/52 (75.00%), Query Frame = -2 
Query:  309 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQL 464
            MALTLRS TSFI LKD++S KA D +SS + FAQM PSC L+ K+  QG QL
Sbjct:    1 MALTLRSSTSFIILKDSKSLKAPDGISSTISFAQMMPSCSLRVKNSTQGAQL 52

HSP 2 Score: 31.5722 bits (70), Expect = 2.209e-13
Identity = 15/26 (57.69%), Postives = 17/26 (65.38%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            KSAG+      D WWGLVEK+GP  Y
Sbjct:  116 KSAGVV-GVMVDVWWGLVEKDGPLIY 140

HSP 3 Score: 21.557 bits (44), Expect = 2.209e-13
Identity = 18/51 (35.29%), Postives = 24/51 (47.06%), Query Frame = -1 
Query:  133 LEDRRNDKWVKDFTGERKLTGNERFQVTLPVFCDACH*DTVSLGGHLNTVR 285
            LE  +++KW K        T N      +PVF      DT++LGG LN  R
Sbjct:   60 LEGSKSNKWEKVNEISIPQTSNGP---KVPVFV-MLPLDTITLGGKLNRPR 106
[Close]
BLAST of DW043271 vs. TrEMBL
Match: Q5F305|Q5F305_SOYBN (Beta-amylase OS=Glycine max GN=bmy2 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 1.006e-10
Identity = 28/59 (47.46%), Postives = 38/59 (64.41%), Query Frame = -2 
Query:  297 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSC---RLKAKSLMQGTQLLQEK 464
            MALTLRS TSFIN K+T+  K  D   ++V F++ +PS    RL+AK+ MQ     +EK
Sbjct:    1 MALTLRSSTSFINQKETKVLKTSDNAPAIVSFSKFKPSSSLFRLRAKNSMQEAHHTREK 59

HSP 2 Score: 38.5058 bits (88), Expect = 1.006e-10
Identity = 18/26 (69.23%), Postives = 19/26 (73.08%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            KSAG+      DAWWGLVEKEGP KY
Sbjct:  109 KSAGVE-GVMVDAWWGLVEKEGPLKY 133

HSP 3 Score: 20.7866 bits (42), Expect = 1.006e-10
Identity = 14/45 (31.11%), Postives = 21/45 (46.67%), Query Frame = -1 
Query:  133 DKWVKDFTGERKLTGNERFQVTLPVFCDACH*DTVSLGGHLNTVR 267
            +KW K        + N+  +V + V       DTV++GG LN  R
Sbjct:   58 EKWEKVLAPSVAHSHNDSKRVPVYVMLPL---DTVTMGGSLNKPR 99
[Close]
BLAST of DW043271 vs. TrEMBL
Match: A5AGI9|A5AGI9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_032500 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.661e-10
Identity = 45/126 (35.71%), Postives = 57/126 (45.24%), Query Frame = -2 
Query:   87 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQLLQEKNLEFWKTGGTISG*KTSRANGSSRVMNDSR*RCLCSVMHATETRYLWGGT*TQSVRADRDASLMALEECG 464
            MALTLRS TSFIN KDT+  K  D +SS + F   +P C ++AK   Q   L  E  +          G K    +G     N +  R    VM   +T    G       RA  +ASLMAL+  G
Sbjct:    1 MALTLRSSTSFINPKDTKGLKTPD-ISSSIFFTPSKPPCSIRAKISTQEAHLCHENAM-------VSEGRKNQVLHGLPTPHNKNGSRVPVFVMLPLDTVSFGGN--LNKPRA-MNASLMALKSAG 115

HSP 2 Score: 37.7354 bits (86), Expect = 2.661e-10
Identity = 17/26 (65.38%), Postives = 19/26 (73.08%), Query Frame = -3 
Query:   20 KSAGIRRSDECDAWWGLVEKEGPSKY 97
            KSAG+      DAWWGLVEK+GP KY
Sbjct:  112 KSAGVE-GVMVDAWWGLVEKDGPMKY 136
[Close]
BLAST of DW043271 vs. TrEMBL
Match: Q94EU9|Q94EU9_SOLTU (Beta-amylase PCT-BMYI OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.700e-9
Identity = 35/62 (56.45%), Postives = 41/62 (66.13%), Query Frame = -2 
Query:  279 MALTLRSPTSFINLKDTRSFKAVDEVSSMVCFAQMRPSCRLKAKSLMQGTQLLQEKNLEFWK 464
            M LTL+S  SFIN K+T+  KA DE   MV FAQ +PSCRL AKS MQ  QL  E+ +E  K
Sbjct:    1 MTLTLQSSASFINFKETKGVKAPDEFLGMVSFAQAKPSCRLVAKSSMQEAQLSHERIMEVKK 62
The following BLAST results are available for this feature:
BLAST of DW043271 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
POPTR_0003s08360.11.624e-1639.10[more]
POPTR_0003s03330.12.598e-1643.53[more]
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BLAST of DW043271 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1
Match NameE-valueIdentityDescription
ppa003812m2.681e-2263.08[more]
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BLAST of DW043271 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 8
Match NameE-valueIdentityDescription
B9RUR8|B9RUR8_RICCO3.098e-1742.86Beta-amylase, putative OS=Ricinus communis GN=RCOM... [more]
D0VBH4|D0VBH4_EUPES5.932e-1565.38Beta-amylase 1 OS=Euphorbia esula GN=BAM1 PE=2 SV=... [more]
A9PGR1|A9PGR1_POPTR1.676e-1439.10Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9MTV8|B9MTV8_POPTR2.709e-1443.53Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D0VBH5|D0VBH5_EUPES2.209e-1363.46Beta-amylase 2 OS=Euphorbia esula GN=BAM2 PE=2 SV=... [more]
Q5F305|Q5F305_SOYBN1.006e-1047.46Beta-amylase OS=Glycine max GN=bmy2 PE=2 SV=1[more]
A5AGI9|A5AGI9_VITVI2.661e-1035.71Putative uncharacterized protein OS=Vitis vinifera... [more]
Q94EU9|Q94EU9_SOLTU1.700e-956.45Beta-amylase PCT-BMYI OS=Solanum tuberosum PE=2 SV... [more]
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Properties
Property NameValue
Singlet in analysisVaccinium corymbosum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>DW043271 ID=DW043271; Name=DW043271; organism=Vaccinium corymbosum; type=EST; length=519bp
AGCGTGGTCGCGTGCCGAGGTACTTGGAAGGCCCTTCTTTCTCCACCAAT
CCCCACCAAGCGTCACACTCATCACTCCTTCGTATTCCCGCACTCTTCTA
ATGCCATCAAACTGGCATCTCTATCTGCTCTTACGGACTGTGTTCAAGTG
CCCCCCCAAAGATACCGTGTCTCAGTGGCATGCATCACAGAACACAGGCA
GCGTTACCTGGAATCGTTCATTACCCGTGAGCTTCCGTTCGCCCGTGAAG
TCTTTTACCCACTTATCGTTCCTCCTGTCTTCCAAAATTCAAGGTTTTTT
TCTTGCAAGAGTTGAGTTCCTTGCATCAAGCTCTTGGCTTTAAGCCTGCA
TGATGGCCTCATTTGTGCAAAACAAACCATGCTGGAGACTTCATCAACTG
CTTTGAAACTTCTTGTATCTTTTAAATTGATAAAAGAAGTTGGAGAACGC
AGTGTCAAAGCCATATTTTGATCTTTGTTTTGAAGAGATACCCGCGTACC
TCGGCCGCGACCACGCTAA
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