CF810748 | GDV

EST Overview


Name	CF810748
Unique Name	CF810748
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	603

Libraries


Library Name	Type
LIBEST_014530 cDNA cold acclimated Bluecrop library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18
KEGG analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of CF810748 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0009s05180.2 ()

HSP 1 Score: 98.2117 bits (243), Expect = 4.051e-21
Identity = 43/57 (75.44%), Postives = 54/57 (94.74%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV LVRG+VKDV+PQK++L+DGT+VPYGLLVWSTGVGPS F+K+LEL K+PGGR
Sbjct:  284 TKSGVHLVRGIVKDVKPQKLILTDGTEVPYGLLVWSTGVGPSSFVKSLELSKSPGGR 340

[Close]

BLAST of CF810748 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0009s05180.1 ()

HSP 1 Score: 98.2117 bits (243), Expect = 4.051e-21
Identity = 43/57 (75.44%), Postives = 54/57 (94.74%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV LVRG+VKDV+PQK++L+DGT+VPYGLLVWSTGVGPS F+K+LEL K+PGGR
Sbjct:  284 TKSGVHLVRGIVKDVKPQKLILTDGTEVPYGLLVWSTGVGPSSFVKSLELSKSPGGR 340

[Close]

BLAST of CF810748 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0004s03640.1 ()

HSP 1 Score: 95.5153 bits (236), Expect = 2.626e-20
Identity = 44/57 (77.19%), Postives = 53/57 (92.98%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV L+RGVVK+V P+KIVLSD T+VPYGLLVWSTGVGPS F+K+L+LPKAPGGR
Sbjct:  341 TKSGVSLMRGVVKEVHPKKIVLSDETNVPYGLLVWSTGVGPSQFVKSLDLPKAPGGR 397

[Close]

BLAST of CF810748 vs. P. persica 1.0 peptide
Match: ppa004595m ()

HSP 1 Score: 98.5969 bits (244), Expect = 2.197e-21
Identity = 44/57 (77.19%), Postives = 53/57 (92.98%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGVRLVRG+VKDV+ QKI+L+DGT+VPYGLLVWSTGVGPSP + +L LPKAPGGR
Sbjct:  297 TKSGVRLVRGIVKDVKAQKIILNDGTEVPYGLLVWSTGVGPSPLVNSLPLPKAPGGR 353

[Close]

BLAST of CF810748 vs. P. persica 1.0 peptide
Match: ppa003771m ()

HSP 1 Score: 95.9005 bits (237), Expect = 1.424e-20
Identity = 43/57 (75.44%), Postives = 53/57 (92.98%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++ GVRL+RGVVK+V P+KIVL+DGTDVPYGLLVWSTGVGPS F+K+L+LPK+ GGR
Sbjct:  345 TKCGVRLMRGVVKEVHPEKIVLNDGTDVPYGLLVWSTGVGPSEFVKSLDLPKSAGGR 401

[Close]

BLAST of CF810748 vs. TrEMBL
Match: C3VXF8|C3VXF8_ACTDE (Putative mitochondrial type II NAD(P)H dehydrogenase (Fragment) OS=Actinidia deliciosa GN=NDA1 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 7.159e-23
Identity = 51/56 (91.07%), Postives = 55/56 (98.21%), Query Frame = 1

Query:  361 QSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            +SGVRLVRG+VKDVQPQKI+LSDGT VPYGLLVWSTGVGPSPF+KNLELPKAPGGR
Sbjct:  183 KSGVRLVRGIVKDVQPQKIILSDGTAVPYGLLVWSTGVGPSPFVKNLELPKAPGGR 238

[Close]

BLAST of CF810748 vs. TrEMBL
Match: B9HP27|B9HP27_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_203252 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 4.802e-19
Identity = 43/57 (75.44%), Postives = 54/57 (94.74%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV LVRG+VKDV+PQK++L+DGT+VPYGLLVWSTGVGPS F+K+LEL K+PGGR
Sbjct:  248 TKSGVHLVRGIVKDVKPQKLILTDGTEVPYGLLVWSTGVGPSSFVKSLELSKSPGGR 304

[Close]

BLAST of CF810748 vs. TrEMBL
Match: A9PHN0|A9PHN0_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 4.802e-19
Identity = 43/57 (75.44%), Postives = 54/57 (94.74%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV LVRG+VKDV+PQK++L+DGT+VPYGLLVWSTGVGPS F+K+LEL K+PGGR
Sbjct:  284 TKSGVHLVRGIVKDVKPQKLILTDGTEVPYGLLVWSTGVGPSSFVKSLELSKSPGGR 340

[Close]

BLAST of CF810748 vs. TrEMBL
Match: B9SI39|B9SI39_RICCO (Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial, putative OS=Ricinus communis GN=RCOM_0612620 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 6.272e-19
Identity = 43/57 (75.44%), Postives = 55/57 (96.49%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV+LVRG+VKDV+PQK+VLSDG++VPYGLLVWSTGVGPS  +K+L+LPK+PGGR
Sbjct:  294 TKSGVQLVRGIVKDVKPQKLVLSDGSEVPYGLLVWSTGVGPSHLVKSLDLPKSPGGR 350

[Close]

BLAST of CF810748 vs. TrEMBL
Match: B9S604|B9S604_RICCO (Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial, putative OS=Ricinus communis GN=RCOM_1062920 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 8.191e-19
Identity = 43/57 (75.44%), Postives = 53/57 (92.98%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGVRL RGVVK+V P+K+ LSDGT+VPYGLLVWSTGVGPS F+K+L+LPK+PGGR
Sbjct:  341 TKSGVRLARGVVKEVHPKKLALSDGTEVPYGLLVWSTGVGPSQFVKSLDLPKSPGGR 397

[Close]

BLAST of CF810748 vs. TrEMBL
Match: Q5JC60|Q5JC60_PEA (Putative NADH-dehydrogenase (Fragment) OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 3.113e-18
Identity = 45/57 (78.95%), Postives = 50/57 (87.72%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGVR VRGVVK+V PQKIVLSDGT VPYGLLVWSTGVGPS F+K L LP +PGGR
Sbjct:  186 TKSGVRFVRGVVKEVHPQKIVLSDGTKVPYGLLVWSTGVGPSEFVKKLYLPVSPGGR 242

[Close]

BLAST of CF810748 vs. TrEMBL
Match: B9H238|B9H238_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_831073 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 3.113e-18
Identity = 44/57 (77.19%), Postives = 53/57 (92.98%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV L+RGVVK+V P+KIVLSD T+VPYGLLVWSTGVGPS F+K+L+LPKAPGGR
Sbjct:  246 TKSGVSLMRGVVKEVHPKKIVLSDETNVPYGLLVWSTGVGPSQFVKSLDLPKAPGGR 302

[Close]

BLAST of CF810748 vs. TrEMBL
Match: Q8L5V3|Q8L5V3_ARATH (Putative NADH dehydrogenase (Ubiquinone oxidoreductase) OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 4.065e-18
Identity = 40/57 (70.18%), Postives = 53/57 (92.98%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV+LVRG+VK+V+PQK++L DGT+VPYGLLVWSTGVGPS F+++L+ PK PGGR
Sbjct:  306 NKSGVKLVRGIVKEVKPQKLILDDGTEVPYGLLVWSTGVGPSSFVRSLDFPKDPGGR 362

[Close]

BLAST of CF810748 vs. TrEMBL
Match: D7KGZ8|D7KGZ8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_887930 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 4.065e-18
Identity = 40/57 (70.18%), Postives = 53/57 (92.98%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV+LVRG+VK+V+PQK++L DGT+VPYGLLVWSTGVGPS F+++L+ PK PGGR
Sbjct:  305 NKSGVKLVRGIVKEVKPQKLILDDGTEVPYGLLVWSTGVGPSSFVRSLDFPKDPGGR 361

[Close]

BLAST of CF810748 vs. TrEMBL
Match: O80874|O80874_ARATH (Putative NADH dehydrogenase (Ubiquinone oxidoreductase) OS=Arabidopsis thaliana GN=At2g29990 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 5.310e-18
Identity = 42/57 (73.68%), Postives = 51/57 (89.47%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGVR VRG+VKDVQ QK++L DGT+VPYGLLVWSTGVGPSPF+++L LPK P GR
Sbjct:  304 NKSGVRFVRGIVKDVQSQKLILDDGTEVPYGLLVWSTGVGPSPFVRSLGLPKDPTGR 360

[Close]

BLAST of CF810748 vs. Vitis vinifera peptide
Match: GSVIVT01024722001 (assembled CDS)

HSP 1 Score: 94.7449 bits (234), Expect = 2.715e-20
Identity = 41/57 (71.93%), Postives = 54/57 (94.74%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGVRLVRG+VKDV+  KI+L++GT+VPYGLLVWSTGVGPS F+K++E+PK+PGGR
Sbjct:  295 TKSGVRLVRGIVKDVKVDKIILNNGTEVPYGLLVWSTGVGPSSFVKSMEVPKSPGGR 351

[Close]

BLAST of CF810748 vs. Vitis vinifera peptide
Match: GSVIVT01020362001 (assembled CDS)

HSP 1 Score: 91.6633 bits (226), Expect = 2.298e-19
Identity = 40/57 (70.18%), Postives = 53/57 (92.98%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGVRLVRG+VKDV+  KI+L++GT+VPYGLLVWST VGPS F+K++E+PK+PGGR
Sbjct:   74 TKSGVRLVRGIVKDVKVDKIILNNGTEVPYGLLVWSTRVGPSSFVKSIEVPKSPGGR 130

[Close]

BLAST of CF810748 vs. Vitis vinifera peptide
Match: GSVIVT01005840001 (assembled CDS)

HSP 1 Score: 88.9669 bits (219), Expect = 1.490e-18
Identity = 41/57 (71.93%), Postives = 49/57 (85.96%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV   RGVVK+V  +KIVLSDGTDVPYGLLVWSTGVGPS F+K+L + K+PGGR
Sbjct:  341 TKSGVHFKRGVVKEVHAKKIVLSDGTDVPYGLLVWSTGVGPSEFVKSLNVSKSPGGR 397

[Close]

BLAST of CF810748 vs. TAIR peptide
Match: AT2G29990.1 (| Symbols: NDA2 | alternative NAD(P)H dehydrogenase 2 | chr2:12793562-12795913 REVERSE LENGTH=508)

HSP 1 Score: 94.7449 bits (234), Expect = 3.946e-20
Identity = 42/57 (73.68%), Postives = 51/57 (89.47%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGVR VRG+VKDVQ QK++L DGT+VPYGLLVWSTGVGPSPF+++L LPK P GR
Sbjct:  304 NKSGVRFVRGIVKDVQSQKLILDDGTEVPYGLLVWSTGVGPSPFVRSLGLPKDPTGR 360

[Close]

BLAST of CF810748 vs. TAIR peptide
Match: AT1G07180.1 (| Symbols: ATNDI1, NDA1 | alternative NAD(P)H dehydrogenase 1 | chr1:2204414-2206773 FORWARD LENGTH=510)

HSP 1 Score: 92.4337 bits (228), Expect = 1.958e-19
Identity = 39/57 (68.42%), Postives = 52/57 (91.23%), Query Frame = 1

Query:  358 SQSGVRLVRGVVKDVQPQKIVLSDGTDVPYGLLVWSTGVGPSPFIKNLELPKAPGGR 528
            ++SGV+LVRG+VK+V+PQK++L DGT+VPYG LVWSTGVGPS F+++L+ PK PGGR
Sbjct:  306 NKSGVKLVRGIVKEVKPQKLILDDGTEVPYGPLVWSTGVGPSSFVRSLDFPKDPGGR 362

The following BLAST results are available for this feature:

BLAST of CF810748 vs. Populus trichocarpa peptide v2.0
BLAST of CF810748 vs. P. persica 1.0 peptide
BLAST of CF810748 vs. TrEMBL
BLAST of CF810748 vs. Vitis vinifera peptide
BLAST of CF810748 vs. TAIR peptide

BLAST of CF810748 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 3


Match Name	E-value	Identity
POPTR_0009s05180.2	4.051e-21	75.44	[more]
POPTR_0009s05180.1	4.051e-21	75.44	[more]
POPTR_0004s03640.1	2.626e-20	77.19	[more]

BLAST of CF810748 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
ppa004595m	2.197e-21	77.19		[more]
ppa003771m	1.424e-20	75.44		[more]

BLAST of CF810748 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
C3VXF8\|C3VXF8_ACTDE	7.159e-23	91.07	Putative mitochondrial type II NAD(P)H dehydrogena...	[more]
B9HP27\|B9HP27_POPTR	4.802e-19	75.44	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
A9PHN0\|A9PHN0_POPTR	4.802e-19	75.44	Putative uncharacterized protein OS=Populus tricho...	[more]
B9SI39\|B9SI39_RICCO	6.272e-19	75.44	Rotenone-insensitive NADH-ubiquinone oxidoreductas...	[more]
B9S604\|B9S604_RICCO	8.191e-19	75.44	Rotenone-insensitive NADH-ubiquinone oxidoreductas...	[more]
Q5JC60\|Q5JC60_PEA	3.113e-18	78.95	Putative NADH-dehydrogenase (Fragment) OS=Pisum sa...	[more]
B9H238\|B9H238_POPTR	3.113e-18	77.19	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q8L5V3\|Q8L5V3_ARATH	4.065e-18	70.18	Putative NADH dehydrogenase (Ubiquinone oxidoreduc...	[more]
D7KGZ8\|D7KGZ8_ARALY	4.065e-18	70.18	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
O80874\|O80874_ARATH	5.310e-18	73.68	Putative NADH dehydrogenase (Ubiquinone oxidoreduc...	[more]

BLAST of CF810748 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 3


Match Name	E-value	Identity	Description
GSVIVT01024722001	2.715e-20	71.93	assembled CDS	[more]
GSVIVT01020362001	2.298e-19	70.18	assembled CDS	[more]
GSVIVT01005840001	1.490e-18	71.93	assembled CDS	[more]

BLAST of CF810748 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G29990.1	3.946e-20	73.68	\| Symbols: NDA2 \| alternative NAD(P)H dehydrogenas...	[more]
AT1G07180.1	1.958e-19	68.42	\| Symbols: ATNDI1, NDA1 \| alternative NAD(P)H deh...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.50.50.60	no description	coord: 27..65 score: 0.0
None	No IPR available	PANTHER	PTHR22915	NADH DEHYDROGENASE-RELATED	coord: 28..84 score: 4.8
None	No IPR available	PANTHER	PTHR22915:SF4	NADH DEHYDROGENASE-RELATED	coord: 28..84 score: 4.8

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF810748 ID=CF810748; Name=CF810748; organism=Vaccinium corymbosum; type=EST; length=603bp
GAAAGTGTGTCTCTGCGGAATTCTGTGGGCATGAATAGAGATTTTTTTCA
TCTTTGGATTTCATTGACTAGAGGAGCAAATTTAGAAGCTATTGTTGAAA
TTAACGAGTGAACTATCATTTAAGCAATGTCCTAACTTGGAAGTGTCGCA
TCTGTCGCTCTTGTCTGTGGGCATCCTGGTAATTAGAGGTCAAGTCTATG
TTACCTGGAATGAGAGATATATGATACAAGGAATGGAAATGGAAACATGC
AGGAAACATAACATTCCTCTAGTTAGCTGTTCTTAGTCACAGATGTCATC
CCTTGTTGCATGTTGTTATGTTTCTTTATCCTGATTGGTTCATCTAACTC
CATTCATTCTCAGTCAGGAGTTCGTCTTGTTCGTGGGGTTGTTAAGGATG
TTCAACCTCAGAAGATAGTTCTCAGTGATGGTACTGATGTTCCTTACGGA
TTGCTAGTATGGTCTACTGGTGTTGGTCCCTCCCCTTTCATTAAAAATCT
TGAGCTTCCAAAAGCTCCGGGAGGAAGGTAAACTCTGAATTTTGTTTGGC
CACTCATATTTGCTGTATGCTTTCCCATTCAACTGTCAAACTGNNAACAT
GTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **KEGG ORTHOLOGY**
Term	Definition
K03885	ndh; NADH dehydrogenase [EC:1.6.99.3]

Vocabulary: **KEGG PATHWAY**
Term	Definition
ko00190	Oxidative phosphorylation

Vocabulary: **INTERPRO**
Term	Definition
noIPR	unintegrated