CF810814 | GDV

EST Overview


Name	CF810814
Unique Name	CF810814
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	639

Libraries


Library Name	Type
LIBEST_014530 cDNA cold acclimated Bluecrop library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18
KEGG analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB2_PEA (Aspartate carbamoyltransferase 2, chloroplastic OS=Pisum sativum GN=PYRB2 PE=2 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 6.588e-40
Identity = 63/72 (87.50%), Postives = 70/72 (97.22%), Query Frame = 1

Query:  202 DGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            +G KFQLDDV+EAQQFDRDIL+AIFEVAR+MEKIE+NSP SQILKG+LMATLFYEPSTRTRLSFESAM+RLG
Sbjct:   73 EGQKFQLDDVVEAQQFDRDILNAIFEVARDMEKIERNSPESQILKGYLMATLFYEPSTRTRLSFESAMRRLG 144

HSP 2 Score: 55.8398 bits (133), Expect = 6.588e-40
Identity = 27/49 (55.10%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSD+IV+RHFESG          IP +NAG
Sbjct:  156 FSSAAKGETLEDTIRTVEGYSDLIVLRHFESGAARRAATIAGIPIVNAG 204

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB3_PEA (Aspartate carbamoyltransferase 3, chloroplastic OS=Pisum sativum GN=PYRB3 PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 2.472e-39
Identity = 62/72 (86.11%), Postives = 69/72 (95.83%), Query Frame = 1

Query:  202 DGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            +G KFQLDDVIEAQQFDRDIL+AIFE+AR+ME IE+NSP SQILKG+LMATLFYEPSTRTRLSFESAM+RLG
Sbjct:   79 EGQKFQLDDVIEAQQFDRDILNAIFEIARDMENIERNSPESQILKGYLMATLFYEPSTRTRLSFESAMRRLG 150

HSP 2 Score: 55.8398 bits (133), Expect = 2.472e-39
Identity = 27/49 (55.10%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSD+IV+RHFESG          IP +NAG
Sbjct:  162 FSSAAKGETLEDTIRTVEGYSDLIVLRHFESGAARRAAAIAGIPIVNAG 210

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB1_PEA (Aspartate carbamoyltransferase 1, chloroplastic OS=Pisum sativum GN=PYRB1 PE=2 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 7.124e-39
Identity = 72/113 (63.72%), Postives = 83/113 (73.45%), Query Frame = 1

Query:   85 PSTVSFNVKSINWEQSGRLLPRNRFQCQA--LDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            PS+ S ++ S    Q G +  +++  C A  L V+S      G KFQLDDVIEAQQFDR+ LSAIFEVAR ME I  NS GSQ+LKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   36 PSSCSMSISSCG--QFG-VSEKSKLLCGAGALQVESAPLFSVGQKFQLDDVIEAQQFDRETLSAIFEVARSMENIRGNSSGSQMLKGYLMATLFYEPSTRTRLSFESAMKRLG 145

HSP 2 Score: 57.3806 bits (137), Expect = 7.124e-39
Identity = 29/49 (59.18%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIV+RHFESG         +IP INAG
Sbjct:  157 FSSAAKGETLEDTIRTVEGYSDIIVLRHFESGAARRAAATANIPVINAG 205

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB_ARATH (Aspartate carbamoyltransferase, chloroplastic OS=Arabidopsis thaliana GN=PYRB PE=2 SV=2)

HSP 1 Score: 119.013 bits (297), Expect = 1.307e-37
Identity = 72/139 (51.80%), Postives = 90/139 (64.75%), Query Frame = 1

Query:    7 PKTIKCYGEFLPYFSSHFAQSPASTPPSTVSFNVKSINWEQSGRLLPRN--RFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            PK + C  EF     S F  S         S ++K    + +   L RN    +C A+   ++    +  KF+L DVIE +QFDR++LSAIF+VAREMEKIEK+S  S+ILKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   19 PKALACSSEFPINLPSPFESSKICLTSFPASRDLK----KNATLNLTRNVGPVRCHAMQAGTR----ELKKFELSDVIEGKQFDREMLSAIFDVAREMEKIEKSSSQSEILKGYLMATLFYEPSTRTRLSFESAMKRLG 149

HSP 2 Score: 58.5362 bits (140), Expect = 1.307e-37
Identity = 30/49 (61.22%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG         +IP INAG
Sbjct:  161 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARKAAATANIPVINAG 209

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB_PYRHO (Aspartate carbamoyltransferase OS=Pyrococcus horikoshii GN=pyrB PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.508e-16
Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 1

Query:  226 DVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            DVI  + F ++ +  +   A  +EK  K     +  KG ++ATLF+EPSTRTRLSFESAM RLG
Sbjct:    7 DVISIRDFSKEDIEVVLSTAERLEKEMKEKGQLEYAKGKILATLFFEPSTRTRLSFESAMHRLG 70

HSP 2 Score: 43.5134 bits (101), Expect = 1.508e-16
Identity = 22/50 (44.00%), Postives = 29/50 (58.00%), Query Frame = 3

Query:  450 SFRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            S   +++   L DTI+TVE YSD+IV+RH + G          IP INAG
Sbjct:   80 STSSVKKGESLRDTIKTVEQYSDVIVIRHPKEGAARLAAEVADIPVINAG 129

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB_PYRIL (Aspartate carbamoyltransferase OS=Pyrobaculum islandicum (strain DSM 4184 / JCM 9189) GN=pyrB PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.312e-16
Identity = 39/73 (53.42%), Postives = 49/73 (67.12%), Query Frame = 1

Query:  226 DVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG------WGS 426
            DVI A+ F+R  L  +FE+A+ MEK  K+    + LKG +MA  F+EPSTRTRLSFE AMKRLG      WG+
Sbjct:    6 DVISARDFNRVDLEELFEMAKYMEKYAKSRV--EFLKGKIMAVAFFEPSTRTRLSFEVAMKRLGGDVIGFWGA 76

HSP 2 Score: 37.3502 bits (85), Expect = 3.312e-16
Identity = 19/46 (41.30%), Postives = 25/46 (54.35%), Query Frame = 3

Query:  462 LQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            +++   L DTIR ++ YSDIIV+RH   G           P INAG
Sbjct:   81 VEKGETLADTIRMLDAYSDIIVIRHKYEGAAKLAAEVAESPVINAG 126

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB_PYRFU (Aspartate carbamoyltransferase OS=Pyrococcus furiosus GN=pyrB PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 7.267e-16
Identity = 30/64 (46.88%), Postives = 41/64 (64.06%), Query Frame = 1

Query:  226 DVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            DVI  + F ++ +  +   A  +EK  K     +  +G ++ATLF+EPSTRTRLSFESAM RLG
Sbjct:    7 DVISIRDFSKEDIEIVLSTAERLEKELKEKGQLEYARGKILATLFFEPSTRTRLSFESAMHRLG 70

HSP 2 Score: 43.1282 bits (100), Expect = 7.267e-16
Identity = 22/50 (44.00%), Postives = 29/50 (58.00%), Query Frame = 3

Query:  450 SFRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            S   +++   L DTI+TVE YSD+IV+RH + G          IP INAG
Sbjct:   80 STSSVKKGESLADTIKTVEQYSDVIVIRHPKEGAARLAAEVAEIPVINAG 129

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB_METVS (Aspartate carbamoyltransferase OS=Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) GN=pyrB PE=3 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 7.272e-16
Identity = 33/63 (52.38%), Postives = 42/63 (66.67%), Query Frame = 1

Query:  229 VIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            +I  +   RD +  I E + +ME +      S IL G ++ATLFYEPSTRTRLSFE+AMKRLG
Sbjct:    4 LISMRDIGRDDILRILEESEKMEAVLNEKGHSDILNGKILATLFYEPSTRTRLSFETAMKRLG 66

HSP 2 Score: 40.0466 bits (92), Expect = 7.272e-16
Identity = 18/40 (45.00%), Postives = 23/40 (57.50%), Query Frame = 3

Query:  480 LEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            L DTI+ + GYSD+IV+RH   G          +P INAG
Sbjct:   86 LTDTIKVISGYSDLIVIRHPSEGAARLSSEVSGVPVINAG 125

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB_CLOD6 (Aspartate carbamoyltransferase OS=Clostridium difficile (strain 630) GN=pyrB PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.194e-15
Identity = 33/64 (51.56%), Postives = 47/64 (73.44%), Query Frame = 1

Query:  226 DVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            ++I+ + F  + +  I E+A+++  I+  S  S+I +G L+ATLFYEPSTRTRLSFESAM RLG
Sbjct:    6 NLIQPEDFSIEEIDEILELAQKI--IDNPSKYSRICEGKLLATLFYEPSTRTRLSFESAMNRLG 67

HSP 2 Score: 40.4318 bits (93), Expect = 1.194e-15
Identity = 20/40 (50.00%), Postives = 23/40 (57.50%), Query Frame = 3

Query:  480 LEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            L DT+R V GY DII MRH +SG          +P INAG
Sbjct:   87 LGDTMRIVSGYVDIIAMRHPQSGAASEAARYTEVPFINAG 126

HSP 3 Score: 21.557 bits (44), Expect = 1.194e-15
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 2

Query:  455 SSAAKGETLG 484
            SSA+KGETLG
Sbjct:   79 SSASKGETLG 88

[Close]

BLAST of CF810814 vs. DB:swissprot:display
Match: PYRB_META3 (Aspartate carbamoyltransferase OS=Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) GN=pyrB PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.594e-15
Identity = 32/55 (58.18%), Postives = 43/55 (78.18%), Query Frame = 1

Query:  253 RDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            ++IL+ I E A +ME++  +   S I+ G ++ATLFYEPSTRTRLSFE+AMKRLG
Sbjct:   13 KEILN-ILEEAEKMEELLNSEKHSNIMNGKILATLFYEPSTRTRLSFETAMKRLG 66

HSP 2 Score: 41.2022 bits (95), Expect = 1.594e-15
Identity = 19/40 (47.50%), Postives = 24/40 (60.00%), Query Frame = 3

Query:  480 LEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            L DTI+ + GY+D+IVMRH   G         +IP INAG
Sbjct:   86 LPDTIKVINGYADLIVMRHPSDGAPRLACEYSNIPIINAG 125

[Close]

BLAST of CF810814 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0001s36730.1 ()

HSP 1 Score: 83.1889 bits (204), Expect = 1.200e-27
Identity = 39/42 (92.86%), Postives = 42/42 (100.00%), Query Frame = 1

Query:  292 MEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            MEK+EKNSPGSQ+LKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:    1 MEKVEKNSPGSQVLKGYLMATLFYEPSTRTRLSFESAMKRLG 42

HSP 2 Score: 57.7658 bits (138), Expect = 1.200e-27
Identity = 29/49 (59.18%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG          +P INAG
Sbjct:   54 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAAKRAAATAEVPVINAG 102

[Close]

BLAST of CF810814 vs. P. persica 1.0 peptide
Match: ppa006647m ()

HSP 1 Score: 147.902 bits (372), Expect = 3.357e-47
Identity = 80/144 (55.56%), Postives = 101/144 (70.14%), Query Frame = 1

Query:    1 SAPKTIKCYGEFLPYFSSHFAQSPASTPPSTVSF-----NVKSINWEQSGRLLPRNRFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            S   T  CY EF   +S+   +  + +    ++      N +S  + Q+ +   +N F C+ L +++  S   GNKFQL DVIE+QQFDRD+LSAIFEVAR+MEKIEKNSPGSQILKG+LMATLFYEPSTRTRLSFESAM+RLG
Sbjct:   17 SFQNTNLCYKEFNCRYSNFIRKQSSHSNSKYLTHSRLFPNERSSEFGQNDKFSQKNGFLCRTLKIENLPSFSVGNKFQLTDVIESQQFDRDLLSAIFEVARDMEKIEKNSPGSQILKGYLMATLFYEPSTRTRLSFESAMRRLG 160

HSP 2 Score: 58.151 bits (139), Expect = 3.357e-47
Identity = 30/49 (61.22%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG          IP INAG
Sbjct:  172 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAAKRAAATAGIPVINAG 220

[Close]

BLAST of CF810814 vs. TrEMBL
Match: C6TIY9|C6TIY9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 7.085e-46
Identity = 83/144 (57.64%), Postives = 99/144 (68.75%), Query Frame = 1

Query:    4 APKTIKCYGEF------LPYFSSHFAQSPASTPPSTVSFNVKSINWEQSGRLLPRNRFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            APKT KC   F      + Y   ++ +        ++S N+ S  WEQ+ R   R+   C+AL ++   S   G KFQ DD+IEAQQFDRDILSAIFEVAR ME +EK+SPGSQILKGFLMATLFYEPSTRTRLSFESAM+RLG
Sbjct:   18 APKTSKCPQNFVCCHSKISYIEPNYLKPVCCPNSRSLSMNMSS-KWEQTDR---RDALHCRALKIEPAPSFSVGQKFQFDDIIEAQQFDRDILSAIFEVARNMENVEKDSPGSQILKGFLMATLFYEPSTRTRLSFESAMRRLG 157

HSP 2 Score: 57.7658 bits (138), Expect = 7.085e-46
Identity = 29/49 (59.18%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRT+EGYSDIIVMRHFESG          IP INAG
Sbjct:  169 FSSAAKGETLEDTIRTIEGYSDIIVMRHFESGAARRAAAIADIPIINAG 217

[Close]

BLAST of CF810814 vs. TrEMBL
Match: B9STZ7|B9STZ7_RICCO (Aspartate carbamoyltransferase 2, chloroplast, putative OS=Ricinus communis GN=RCOM_0753060 PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.178e-42
Identity = 74/104 (71.15%), Postives = 87/104 (83.65%), Query Frame = 1

Query:  109 KSINWEQSGRLLPRNRFQCQALDVDSKTSILD-GNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            K I++    R L R+  +C AL++ +   +   GN+FQL+DVIEAQQFDR+IL+AIFEVAREMEKIEKNSPGSQILKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   16 KPISFSPVLRPLIRSGVRCSALEIGNADLLFSTGNRFQLEDVIEAQQFDREILNAIFEVAREMEKIEKNSPGSQILKGYLMATLFYEPSTRTRLSFESAMKRLG 119

HSP 2 Score: 57.7658 bits (138), Expect = 1.178e-42
Identity = 30/49 (61.22%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG          IP INAG
Sbjct:  131 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARRAATTACIPVINAG 179

[Close]

BLAST of CF810814 vs. TrEMBL
Match: Q8L6B8|Q8L6B8_SOLTU (Aspartate carbamoyltransferase OS=Solanum tuberosum GN=pyrB PE=2 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 3.677e-41
Identity = 75/110 (68.18%), Postives = 89/110 (80.91%), Query Frame = 1

Query:  109 KSINWEQSGRLLPR-----NRFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPG--SQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            KS+   ++GR   R     N+FQC+AL++++K+S     KF LDDVIE+QQFDR+ LSAIFEVAREMEKIEKNS G  S+ILKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   31 KSVELFKNGRNQYRMSTNSNKFQCRALEIENKSS----TKFHLDDVIESQQFDRETLSAIFEVAREMEKIEKNSIGGRSEILKGYLMATLFYEPSTRTRLSFESAMKRLG 136

HSP 2 Score: 59.6918 bits (143), Expect = 3.677e-41
Identity = 31/49 (63.27%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG         SIP INAG
Sbjct:  148 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARRAATTASIPIINAG 196

[Close]

BLAST of CF810814 vs. TrEMBL
Match: B9GJE5|B9GJE5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_550113 PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 4.800e-41
Identity = 67/90 (74.44%), Postives = 76/90 (84.44%), Query Frame = 1

Query:  148 RNRFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            R+R   Q L + +  S   G KFQLDD+IEAQQFDR+IL+ IFEVA EMEK+EKNSPGSQ+LKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:    2 RSRIHSQLLGIQNTPSFSAGKKFQLDDLIEAQQFDREILADIFEVAHEMEKVEKNSPGSQVLKGYLMATLFYEPSTRTRLSFESAMKRLG 91

HSP 2 Score: 57.7658 bits (138), Expect = 4.800e-41
Identity = 29/49 (59.18%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG          +P INAG
Sbjct:  103 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAAKRAAATAEVPVINAG 151

[Close]

BLAST of CF810814 vs. TrEMBL
Match: D7LAY5|D7LAY5_ARALY (Chloroplast aspartate carabmoyltransferase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_898446 PE=4 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 5.035e-37
Identity = 66/116 (56.90%), Postives = 82/116 (70.69%), Query Frame = 1

Query:   70 PASTPPSTVSFNVKSINWEQSGRLLPRNRFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            PAS  P       K+  W  +  + P    +C A+   ++    +  KF+L DVIE +QFDR++LSAIF+VAREMEKIEK+S  S+ILKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   47 PASRDPK------KNATWNLTRNVGPIQGIRCHAMQAGTR----ELKKFELSDVIEGKQFDREMLSAIFDVAREMEKIEKSSSQSEILKGYLMATLFYEPSTRTRLSFESAMKRLG 152

HSP 2 Score: 58.5362 bits (140), Expect = 5.035e-37
Identity = 30/49 (61.22%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG         +IP INAG
Sbjct:  164 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARKAAATANIPVINAG 212

[Close]

BLAST of CF810814 vs. TrEMBL
Match: C6TLH8|C6TLH8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 1.889e-36
Identity = 62/83 (74.70%), Postives = 67/83 (80.72%), Query Frame = 1

Query:  169 ALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            AL V+S  S   G KF L DVIEAQQFDR+ L+AIFEVAR ME IE  S  SQ+LKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   68 ALQVESAPSFSMGQKFHLHDVIEAQQFDRETLNAIFEVARSMESIESKSSRSQMLKGYLMATLFYEPSTRTRLSFESAMKRLG 150

HSP 2 Score: 59.6918 bits (143), Expect = 1.889e-36
Identity = 31/49 (63.27%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG         SIP INAG
Sbjct:  162 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAAKRAATTASIPVINAG 210

[Close]

BLAST of CF810814 vs. TrEMBL
Match: Q94A47|Q94A47_ARATH (AT3g20330/MQC12_8 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.890e-36
Identity = 72/139 (51.80%), Postives = 90/139 (64.75%), Query Frame = 1

Query:    7 PKTIKCYGEFLPYFSSHFAQSPASTPPSTVSFNVKSINWEQSGRLLPRN--RFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            PK + C  EF     S F  S         S ++K    + +   L RN    +C A+   ++    +  KF+L DVIE +QFDR++LSAIF+VAREMEKIEK+S  S+ILKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   19 PKALACSSEFPINLPSPFESSKICLTSFPASRDLK----KNATLNLTRNVGPVRCHAMQAGTR----ELKKFELSDVIEGKQFDREMLSAIFDVAREMEKIEKSSSQSEILKGYLMATLFYEPSTRTRLSFESAMKRLG 149

HSP 2 Score: 58.5362 bits (140), Expect = 1.890e-36
Identity = 30/49 (61.22%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG         +IP INAG
Sbjct:  161 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARKAAATANIPVINAG 209

[Close]

BLAST of CF810814 vs. TrEMBL
Match: Q683J9|Q683J9_ARATH (Aspartate carbamoyltransferase OS=Arabidopsis thaliana GN=At3g20330 PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.890e-36
Identity = 72/139 (51.80%), Postives = 90/139 (64.75%), Query Frame = 1

Query:    7 PKTIKCYGEFLPYFSSHFAQSPASTPPSTVSFNVKSINWEQSGRLLPRN--RFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            PK + C  EF     S F  S         S ++K    + +   L RN    +C A+   ++    +  KF+L DVIE +QFDR++LSAIF+VAREMEKIEK+S  S+ILKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   19 PKALACSSEFPINLPSPFESSKICLTSFPASRDLK----KNATLNLTRNVGPVRCHAMQAGTR----ELKKFELSDVIEGKQFDREMLSAIFDVAREMEKIEKSSSQSEILKGYLMATLFYEPSTRTRLSFESAMKRLG 149

HSP 2 Score: 58.5362 bits (140), Expect = 1.890e-36
Identity = 30/49 (61.22%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG         +IP INAG
Sbjct:  161 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARKAAATANIPVINAG 209

[Close]

BLAST of CF810814 vs. TrEMBL
Match: B9V281|B9V281_MEDTR (Plastid aspartate carbamoyltransferase OS=Medicago truncatula GN=PyrB PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 3.206e-36
Identity = 59/73 (80.82%), Postives = 68/73 (93.15%), Query Frame = 1

Query:  199 LDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            L+G KFQLDDVIEAQQFD D L+AIF+VAR+ME IE+NSP SQIL+G+LM+TLFYEPSTRTRLSFESAM+RLG
Sbjct:   78 LEGQKFQLDDVIEAQQFDTDTLNAIFKVARDMENIERNSPESQILEGYLMSTLFYEPSTRTRLSFESAMRRLG 150

HSP 2 Score: 55.8398 bits (133), Expect = 3.206e-36
Identity = 27/49 (55.10%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSD+IV+RHFESG          IP +NAG
Sbjct:  162 FSSAAKGETLEDTIRTVEGYSDLIVLRHFESGAARRAAAIAGIPIVNAG 210

[Close]

BLAST of CF810814 vs. TrEMBL
Match: A9RZX3|A9RZX3_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_121970 PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 9.257e-36
Identity = 57/70 (81.43%), Postives = 67/70 (95.71%), Query Frame = 1

Query:  208 NKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            N+F L DV+EAQQFDRD+L++IFEVAREME++E++S GSQ+LKGFLMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   22 NRFPLQDVLEAQQFDRDLLTSIFEVAREMEEVERDSAGSQLLKGFLMATLFYEPSTRTRLSFESAMKRLG 91

HSP 2 Score: 55.8398 bits (133), Expect = 9.257e-36
Identity = 28/49 (57.14%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LED+IRTVEGYSDIIV+RHFESG         +IP INAG
Sbjct:  103 FSSAAKGETLEDSIRTVEGYSDIIVLRHFESGAAKKAADTANIPVINAG 151

[Close]

BLAST of CF810814 vs. Vitis vinifera peptide
Match: GSVIVT01037706001 (assembled CDS)

HSP 1 Score: 58.151 bits (139), Expect = 2.872e-18
Identity = 30/49 (61.22%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG          IP INAG
Sbjct:   36 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARRAAVTAEIPIINAG 84

HSP 2 Score: 50.447 bits (119), Expect = 2.872e-18
Identity = 24/24 (100.00%), Postives = 24/24 (100.00%), Query Frame = 1

Query:  346 MATLFYEPSTRTRLSFESAMKRLG 417
            MATLFYEPSTRTRLSFESAMKRLG
Sbjct:    1 MATLFYEPSTRTRLSFESAMKRLG 24

[Close]

BLAST of CF810814 vs. TAIR peptide
Match: AT3G20330.1 (| Symbols: PYRB | PYRIMIDINE B | chr3:7090354-7091904 REVERSE LENGTH=390)

HSP 1 Score: 119.013 bits (297), Expect = 1.339e-38
Identity = 72/139 (51.80%), Postives = 90/139 (64.75%), Query Frame = 1

Query:    7 PKTIKCYGEFLPYFSSHFAQSPASTPPSTVSFNVKSINWEQSGRLLPRN--RFQCQALDVDSKTSILDGNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQILKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            PK + C  EF     S F  S         S ++K    + +   L RN    +C A+   ++    +  KF+L DVIE +QFDR++LSAIF+VAREMEKIEK+S  S+ILKG+LMATLFYEPSTRTRLSFESAMKRLG
Sbjct:   19 PKALACSSEFPINLPSPFESSKICLTSFPASRDLK----KNATLNLTRNVGPVRCHAMQAGTR----ELKKFELSDVIEGKQFDREMLSAIFDVAREMEKIEKSSSQSEILKGYLMATLFYEPSTRTRLSFESAMKRLG 149

HSP 2 Score: 58.5362 bits (140), Expect = 1.339e-38
Identity = 30/49 (61.22%), Postives = 32/49 (65.31%), Query Frame = 3

Query:  453 FRQLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXINAG 599
            F    +   LEDTIRTVEGYSDIIVMRHFESG         +IP INAG
Sbjct:  161 FSSAAKGETLEDTIRTVEGYSDIIVMRHFESGAARKAAATANIPVINAG 209

[Close]

BLAST of CF810814 vs. TAIR peptide
Match: AT1G75330.1 (| Symbols: OTC | ornithine carbamoyltransferase | chr1:28266457-28268383 REVERSE LENGTH=375)

HSP 1 Score: 52.7582 bits (125), Expect = 3.745e-9
Identity = 39/122 (31.97%), Postives = 59/122 (48.36%), Query Frame = 1

Query:   58 FAQSPASTPPSTVSFNVKSINWEQSGRLLPRNRFQCQALDVDSKTSILD-GNKFQLDDVIEAQQFDRDILSAIFEVAREMEKIEKNSPGSQI-LKGFLMATLFYEPSTRTRLSFESAMKRLG 417
            F+ S +S  P T      +++       LPR R  CQA  V S +S  D   K  L D +    FD   +  I + A E++ + K+   + +  KG  M+ +F +PS RTR+SFE+    LG
Sbjct:   19 FSSSSSSFFPGTTLRRFSAVSLPSPA--LPRLRVSCQASSVTSPSSPSDVKGKSDLKDFLAIDDFDTATIKTILDKASEVKALLKSGERNYLPFKGKSMSMIFAKPSMRTRVSFETGFFLLG 138

HSP 2 Score: 25.409 bits (54), Expect = 3.745e-9
Identity = 12/45 (26.67%), Postives = 19/45 (42.22%), Query Frame = 3

Query:  459 QLQRERHLEDTIRTVEGYSDIIVMRHFESGXXXXXXXXXSIPXIN 593
            Q+ +     D  R +  Y+DII+ R F            S+P +N
Sbjct:  150 QMGKREETRDVARVLSRYNDIIMARVFAHQDILDLANYSSVPVVN 194

The following BLAST results are available for this feature:

BLAST of CF810814 vs. DB:swissprot:display
BLAST of CF810814 vs. Populus trichocarpa peptide v2.0
BLAST of CF810814 vs. P. persica 1.0 peptide
BLAST of CF810814 vs. TrEMBL
BLAST of CF810814 vs. Vitis vinifera peptide
BLAST of CF810814 vs. TAIR peptide

BLAST of CF810814 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
PYRB2_PEA	6.588e-40	87.50	Aspartate carbamoyltransferase 2, chloroplastic OS...	[more]
PYRB3_PEA	2.472e-39	86.11	Aspartate carbamoyltransferase 3, chloroplastic OS...	[more]
PYRB1_PEA	7.124e-39	63.72	Aspartate carbamoyltransferase 1, chloroplastic OS...	[more]
PYRB_ARATH	1.307e-37	51.80	Aspartate carbamoyltransferase, chloroplastic OS=A...	[more]
PYRB_PYRHO	1.508e-16	48.44	Aspartate carbamoyltransferase OS=Pyrococcus horik...	[more]
PYRB_PYRIL	3.312e-16	53.42	Aspartate carbamoyltransferase OS=Pyrobaculum isla...	[more]
PYRB_PYRFU	7.267e-16	46.88	Aspartate carbamoyltransferase OS=Pyrococcus furio...	[more]
PYRB_METVS	7.272e-16	52.38	Aspartate carbamoyltransferase OS=Methanococcus va...	[more]
PYRB_CLOD6	1.194e-15	51.56	Aspartate carbamoyltransferase OS=Clostridium diff...	[more]
PYRB_META3	1.594e-15	58.18	Aspartate carbamoyltransferase OS=Methanococcus ae...	[more]

BLAST of CF810814 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
POPTR_0001s36730.1	1.200e-27	92.86		[more]

BLAST of CF810814 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
ppa006647m	3.357e-47	55.56		[more]

BLAST of CF810814 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
C6TIY9\|C6TIY9_SOYBN	7.085e-46	57.64	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9STZ7\|B9STZ7_RICCO	1.178e-42	71.15	Aspartate carbamoyltransferase 2, chloroplast, put...	[more]
Q8L6B8\|Q8L6B8_SOLTU	3.677e-41	68.18	Aspartate carbamoyltransferase OS=Solanum tuberosu...	[more]
B9GJE5\|B9GJE5_POPTR	4.800e-41	74.44	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7LAY5\|D7LAY5_ARALY	5.035e-37	56.90	Chloroplast aspartate carabmoyltransferase OS=Arab...	[more]
C6TLH8\|C6TLH8_SOYBN	1.889e-36	74.70	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q94A47\|Q94A47_ARATH	1.890e-36	51.80	AT3g20330/MQC12_8 OS=Arabidopsis thaliana PE=2 SV=...	[more]
Q683J9\|Q683J9_ARATH	1.890e-36	51.80	Aspartate carbamoyltransferase OS=Arabidopsis thal...	[more]
B9V281\|B9V281_MEDTR	3.206e-36	80.82	Plastid aspartate carbamoyltransferase OS=Medicago...	[more]
A9RZX3\|A9RZX3_PHYPA	9.257e-36	81.43	Predicted protein OS=Physcomitrella patens subsp. ...	[more]

BLAST of CF810814 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1


Match Name	E-value	Identity	Description
GSVIVT01037706001	2.872e-18	61.22	assembled CDS	[more]

BLAST of CF810814 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT3G20330.1	1.339e-38	51.80	\| Symbols: PYRB \| PYRIMIDINE B \| chr3:7090354-7091...	[more]
AT1G75330.1	3.745e-9	31.97	\| Symbols: OTC \| ornithine carbamoyltransferase \| ...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006130	Aspartate/ornithine carbamoyltransferase	PROSITE	PS00097	CARBAMOYLTRANSFERASE	coord: 120..127 score
IPR006130	Aspartate/ornithine carbamoyltransferase	SUPERFAMILY	53671	Aspartate/ornithine carbamoyltransferase	coord: 69..140 score: 8.1
IPR006130	Aspartate/ornithine carbamoyltransferase	SUPERFAMILY	53671	Aspartate/ornithine carbamoyltransferase	coord: 10..56 score: 1.9
IPR006132	Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding	PFAM	PF02729	OTCace_N	coord: 76..140 score: 2.5
IPR006132	Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding	PFAM	PF02729	OTCace_N	coord: 9..57 score: 6
None	No IPR available	GENE3D	3.40.50.1370	no description	coord: 71..140 score: 1.1
None	No IPR available	PANTHER	PTHR11405	CARBAMOYLTRANSFERASE RELATED	coord: 71..139 score: 5.6
None	No IPR available	GENE3D	3.40.50.1370	no description	coord: 8..57 score: 1.4
None	No IPR available	PANTHER	PTHR11405	CARBAMOYLTRANSFERASE RELATED	coord: 17..56 score: 2.7
None	No IPR available	SEG	seg	seg	coord: 40..48 score: NAcoord: 57..68 score
None	No IPR available	SEG	seg	seg	coord: 22..30 score
None	No IPR available	SEG	seg	seg	coord: 16..27 score

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF810814 ID=CF810814; Name=CF810814; organism=Vaccinium corymbosum; type=EST; length=639bp
TCTGCTCCTAAAACAATAAAGTGTTACGGAGAGTTCCTTCCCTATTTTTC
GAGTCATTTTGCGCAATCACCTGCCAGCACACCACCCTCAACGGTGTCGT
TCAATGTGAAATCAATAAACTGGGAACAAAGTGGGAGGCTCTTACCAAGG
AATAGGTTCCAATGTCAAGCGCTGGATGTTGACTCTAAAACTTCAATTTT
GGATGGGAACAAGTTTCAACTTGATGACGTGATCGAGGCCCAGCAATTCG
ATAGAGATATTCTTAGTGCTATATTTGAAGTGGCACGTGAGATGGAGAAG
ATCGAGAAAAACTCACCTGGCAGCCAAATTCTTAAGGGGTTTCTAATGGC
TACCCTATTTTACGAGCCATCAACTAGAACTAGGCTCTCTTTTGAATCTG
CTATGAAACGGCTGGGGTGGGGAAGTTTTGACAACAGAAAATGCACGGGA
GTTTTCGTCAGCTGCAAAGGGAGAGACACTTGGAAGATACTATTAGAACT
GTTGAAGGGTACTCGGATATCATAGTGATGAGGCACTTTGAAAGTGGNGC
TGCCCGAANAGCAGCAGCTACTGCCAGTATTCCTGNTATTAATGCTGGGG
NATGGTCCTGGACAACATCCAACTCAAGCTCTTCTAGAB

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006520	amino acid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016597	amino acid binding
GO:0016743	carboxyl- or carbamoyltransferase activity

Vocabulary: **KEGG ORTHOLOGY**
Term	Definition
K00609	pyrB, PYR2; aspartate carbamoyltransferase catalytic subunit [EC:2.1.3.2]

Vocabulary: **KEGG PATHWAY**
Term	Definition
ko00230	Purine metabolism
ko00250	Alanine, aspartate and glutamate metabolism

Vocabulary: **INTERPRO**
Term	Definition
IPR006130	Asp/Orn_carbamoylTrfase
IPR006132	Asp/Orn_carbamoyltranf_P-bd
noIPR	unintegrated