HO054861 | GDV

EST Overview


Name	HO054861
Unique Name	HO054861
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	501

Libraries


Library Name	Type
LIBEST_026368 Short-term aluminum-stress in root cv. Brigitta cDNA-AFLP	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of HO054861 vs. DB:swissprot:display
Match: DRL9_ARATH (Probable disease resistance protein At1g58602 OS=Arabidopsis thaliana GN=At1g58602 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.187e-13
Identity = 38/62 (61.29%), Postives = 43/62 (69.35%), Query Frame = -2

Query:  315 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEFDD 500
            MPLLHTLYIGVCPNLKEL DG      +K     K+ ++ +     EDYYKVQHIPSVEFDD
Sbjct: 1078 MPLLHTLYIGVCPNLKELPDGLRFIYSLKNLIVSKRWKKRL-SEGGEDYYKVQHIPSVEFDD 1138

[Close]

BLAST of HO054861 vs. DB:swissprot:display
Match: DRL11_ARATH (Probable disease resistance protein RDL6/RF9 OS=Arabidopsis thaliana GN=RDL6 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 8.802e-9
Identity = 31/60 (51.67%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CP LK+L DG      +K   ++ K  ++ + +  EDYYKVQHIPSVEF
Sbjct:  990 MPLLHTLRIWNCPKLKQLPDGLRFIYSLKNL-TVPKRWKKRLSKGGEDYYKVQHIPSVEF 1048

[Close]

BLAST of HO054861 vs. DB:swissprot:display
Match: DRL8_ARATH (Putative disease resistance protein At1g58400 OS=Arabidopsis thaliana GN=At1g58400 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.961e-8
Identity = 32/61 (52.46%), Postives = 38/61 (62.30%), Query Frame = -2

Query:  318 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEFD 500
            MP LHTL I  C  LK+L DG      IK  D  KK +E I+    E+YYKVQHIPSV+F+
Sbjct:  847 MPRLHTLTIWNCQKLKQLPDGLRFIYSIKDLDMDKKWKE-ILSEGGEEYYKVQHIPSVKFE 906

[Close]

BLAST of HO054861 vs. TrEMBL
Match: Q0WV58|Q0WV58_ARATH (Putative uncharacterized protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.512e-12
Identity = 38/62 (61.29%), Postives = 43/62 (69.35%), Query Frame = -2

Query:  315 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEFDD 500
            MPLLHTLYIGVCPNLKEL DG      +K     K+ ++ +     EDYYKVQHIPSVEFDD
Sbjct: 1078 MPLLHTLYIGVCPNLKELPDGLRFIYSLKNLIVSKRWKKRL-SEGGEDYYKVQHIPSVEFDD 1138

[Close]

BLAST of HO054861 vs. TrEMBL
Match: B5BRD4|B5BRD4_ARATH (Putative disease resistance protein OS=Arabidopsis thaliana GN=At1g58848 homologue-1 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.855e-8
Identity = 33/62 (53.23%), Postives = 38/62 (61.29%), Query Frame = -2

Query:  315 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEFDD 500
            MP LHTL I  C  LK+L DG      +KK    K+ +E +     EDYYKVQHIPSVEFDD
Sbjct:  992 MPRLHTLTIWNCQKLKQLPDGLRFIYSLKKLIISKRWKERL-SEGGEDYYKVQHIPSVEFDD 1052

[Close]

BLAST of HO054861 vs. TrEMBL
Match: Q570X7|Q570X7_ARATH (Viral resistance protein OS=Arabidopsis thaliana GN=At1g58842 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.121e-7
Identity = 31/60 (51.67%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CP LK+L DG      +K   ++ K  ++ + +  EDYYKVQHIPSVEF
Sbjct:  142 MPLLHTLRIWNCPKLKQLPDGLRFIYSLKNL-TVPKRWKKRLSKGGEDYYKVQHIPSVEF 200

[Close]

BLAST of HO054861 vs. TrEMBL
Match: Q3ECM3|Q3ECM3_ARATH (Putative uncharacterized protein At1g58807.1 OS=Arabidopsis thaliana GN=At1g58807 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.121e-7
Identity = 31/60 (51.67%), Postives = 37/60 (61.67%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MP LHTLYI  CP LK+L DG      +K    I +  +E +    E+YYKVQHIPSVEF
Sbjct:  937 MPFLHTLYIDDCPKLKKLPDGLQFIYSLKNL-KISERWKERLSEGGEEYYKVQHIPSVEF 995

[Close]

BLAST of HO054861 vs. TrEMBL
Match: Q0WNF7|Q0WNF7_ARATH (PRM1 homolog OS=Arabidopsis thaliana GN=At1g59124 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.121e-7
Identity = 31/60 (51.67%), Postives = 37/60 (61.67%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MP LHTLYI  CP LK+L DG      +K    I +  +E +    E+YYKVQHIPSVEF
Sbjct:  631 MPFLHTLYIDDCPKLKKLPDGLQFIYSLKNL-KISERWKERLSEGGEEYYKVQHIPSVEF 689

[Close]

BLAST of HO054861 vs. TrEMBL
Match: Q0WLD0|Q0WLD0_ARATH (Putative viral resistance protein (Fragment) OS=Arabidopsis thaliana GN=At1g58842 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.121e-7
Identity = 31/60 (51.67%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CP LK+L DG      +K   ++ K  ++ + +  EDYYKVQHIPSVEF
Sbjct:  168 MPLLHTLRIWNCPKLKQLPDGLRFIYSLKNL-TVPKRWKKRLSKGGEDYYKVQHIPSVEF 226

[Close]

BLAST of HO054861 vs. TrEMBL
Match: B5BRD6|B5BRD6_ARATH (Putative disease resistance gene (Fragment) OS=Arabidopsis thaliana GN=At1g58848 homologue PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.913e-7
Identity = 31/60 (51.67%), Postives = 37/60 (61.67%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CPNLKEL DG      +K     K+ ++ +     EDYYKVQHIP+V F
Sbjct:  689 MPLLHTLNIDDCPNLKELPDGLRFIYSLKNLKVSKRWKKRL-SEGGEDYYKVQHIPTVIF 747

[Close]

BLAST of HO054861 vs. TrEMBL
Match: B5BRD5|B5BRD5_ARATH (Utative disease resistance protein OS=Arabidopsis thaliana GN=At1g58848 homologue-2 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.913e-7
Identity = 31/60 (51.67%), Postives = 37/60 (61.67%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CPNLKEL DG      +K     K+ ++ +     EDYYKVQHIP+V F
Sbjct:  753 MPLLHTLNIDDCPNLKELPDGLRFIYSLKNLKVSKRWKKRL-SEGGEDYYKVQHIPTVIF 811

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G58602.2 (| Symbols: | LRR and NB-ARC domains-containing disease resistance protein | chr1:21760167-21763765 FORWARD LENGTH=1138)

HSP 1 Score: 75.8702 bits (185), Expect = 1.315e-14
Identity = 38/62 (61.29%), Postives = 43/62 (69.35%), Query Frame = -2

Query:  315 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEFDD 500
            MPLLHTLYIGVCPNLKEL DG      +K     K+ ++ +     EDYYKVQHIPSVEFDD
Sbjct: 1078 MPLLHTLYIGVCPNLKELPDGLRFIYSLKNLIVSKRWKKRL-SEGGEDYYKVQHIPSVEFDD 1138

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G58602.1 (| Symbols: | LRR and NB-ARC domains-containing disease resistance protein | chr1:21760167-21763765 FORWARD LENGTH=1138)

HSP 1 Score: 75.8702 bits (185), Expect = 1.315e-14
Identity = 38/62 (61.29%), Postives = 43/62 (69.35%), Query Frame = -2

Query:  315 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEFDD 500
            MPLLHTLYIGVCPNLKEL DG      +K     K+ ++ +     EDYYKVQHIPSVEFDD
Sbjct: 1078 MPLLHTLYIGVCPNLKELPDGLRFIYSLKNLIVSKRWKKRL-SEGGEDYYKVQHIPSVEFDD 1138

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G59218.2 (| Symbols: | Disease resistance protein (CC-NBS-LRR class) family | chr1:21828398-21831713 FORWARD LENGTH=1049)

HSP 1 Score: 59.6918 bits (143), Expect = 9.749e-10
Identity = 31/60 (51.67%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CP LK+L DG      +K   ++ K  ++ + +  EDYYKVQHIPSVEF
Sbjct:  990 MPLLHTLRIWNCPKLKQLPDGLRFIYSLKNL-TVPKRWKKRLSKGGEDYYKVQHIPSVEF 1048

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G58848.2 (| Symbols: | Disease resistance protein (CC-NBS-LRR class) family | chr1:21792140-21795455 FORWARD LENGTH=1049)

HSP 1 Score: 59.6918 bits (143), Expect = 9.749e-10
Identity = 31/60 (51.67%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CP LK+L DG      +K   ++ K  ++ + +  EDYYKVQHIPSVEF
Sbjct:  990 MPLLHTLRIWNCPKLKQLPDGLRFIYSLKNL-TVPKRWKKRLSKGGEDYYKVQHIPSVEF 1048

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G59218.1 (| Symbols: | Disease resistance protein (CC-NBS-LRR class) family | chr1:21828398-21831713 FORWARD LENGTH=1049)

HSP 1 Score: 59.6918 bits (143), Expect = 9.749e-10
Identity = 31/60 (51.67%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CP LK+L DG      +K   ++ K  ++ + +  EDYYKVQHIPSVEF
Sbjct:  990 MPLLHTLRIWNCPKLKQLPDGLRFIYSLKNL-TVPKRWKKRLSKGGEDYYKVQHIPSVEF 1048

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G58807.1 (| Symbols: | Disease resistance protein (CC-NBS-LRR class) family | chr1:21780574-21783793 FORWARD LENGTH=1017)

HSP 1 Score: 59.6918 bits (143), Expect = 9.749e-10
Identity = 31/60 (51.67%), Postives = 37/60 (61.67%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MP LHTLYI  CP LK+L DG      +K    I +  +E +    E+YYKVQHIPSVEF
Sbjct:  937 MPFLHTLYIDDCPKLKKLPDGLQFIYSLKNL-KISERWKERLSEGGEEYYKVQHIPSVEF 995

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G58848.1 (| Symbols: | Disease resistance protein (CC-NBS-LRR class) family | chr1:21792140-21795455 FORWARD LENGTH=1049)

HSP 1 Score: 59.6918 bits (143), Expect = 9.749e-10
Identity = 31/60 (51.67%), Postives = 39/60 (65.00%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MPLLHTL I  CP LK+L DG      +K   ++ K  ++ + +  EDYYKVQHIPSVEF
Sbjct:  990 MPLLHTLRIWNCPKLKQLPDGLRFIYSLKNL-TVPKRWKKRLSKGGEDYYKVQHIPSVEF 1048

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G58400.1 (| Symbols: | Disease resistance protein (CC-NBS-LRR class) family | chr1:21696165-21699118 REVERSE LENGTH=900)

HSP 1 Score: 58.5362 bits (140), Expect = 2.172e-9
Identity = 32/61 (52.46%), Postives = 38/61 (62.30%), Query Frame = -2

Query:  318 MPLLHTLYIGVCPNLKELRDGAAIYLFIKKFDSIKKMEEEIVGREEEDYYKVQHIPSVEFD 500
            MP LHTL I  C  LK+L DG      IK  D  KK +E I+    E+YYKVQHIPSV+F+
Sbjct:  837 MPRLHTLTIWNCQKLKQLPDGLRFIYSIKDLDMDKKWKE-ILSEGGEEYYKVQHIPSVKFE 896

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT1G59780.1 (| Symbols: | NB-ARC domain-containing disease resistance protein | chr1:21993581-21997691 REVERSE LENGTH=906)

HSP 1 Score: 51.2174 bits (121), Expect = 3.468e-7
Identity = 26/77 (33.77%), Postives = 47/77 (61.04%), Query Frame = -2

Query:  276 MPLLHTLYIGVCPNLKELRDGAAIYLFIKK--FDSIKKMEEEIVGREEEDYYKVQHIPSVEFDD*ECSQQSQSMYIY 500
            MPLLHTL+I  C  LKE+ DG      +K+    + +K+ ++ V +  EDYYK+QH+P + ++  +  + ++ +Y +
Sbjct:  825 MPLLHTLHIVDCKKLKEIPDGLRFISSLKELAIRTNEKVFQKKVSKGGEDYYKMQHVPLIRYNWPQEPENNEVIYSF 901

[Close]

BLAST of HO054861 vs. TAIR peptide
Match: AT5G43470.2 (| Symbols: RPP8, HRT, RCY1 | Disease resistance protein (CC-NBS-LRR class) family | chr5:17463130-17466658 REVERSE LENGTH=908)

HSP 1 Score: 50.8322 bits (120), Expect = 4.529e-7
Identity = 29/62 (46.77%), Postives = 36/62 (58.06%), Query Frame = -2

Query:  321 MPLLHTLYIGVCPNLKELRDGAAIYLFIK--KFDSIKKMEEEIVGREEEDYYKVQHIPSVEF 500
            MP L TL I  C  LKEL DG      +K  K + +K+  +E +    EDYYKVQHIP V+F
Sbjct:  842 MPCLRTLTIDDCKKLKELPDGLKYITSLKELKIEGMKREWKEKLVPGGEDYYKVQHIPDVQF 903

The following BLAST results are available for this feature:

BLAST of HO054861 vs. DB:swissprot:display
BLAST of HO054861 vs. TrEMBL
BLAST of HO054861 vs. TAIR peptide

BLAST of HO054861 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
DRL9_ARATH	1.187e-13	61.29	Probable disease resistance protein At1g58602 OS=A...	[more]
DRL11_ARATH	8.802e-9	51.67	Probable disease resistance protein RDL6/RF9 OS=Ar...	[more]
DRL8_ARATH	1.961e-8	52.46	Putative disease resistance protein At1g58400 OS=A...	[more]

BLAST of HO054861 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 8


Match Name	E-value	Identity	Description
Q0WV58\|Q0WV58_ARATH	1.512e-12	61.29	Putative uncharacterized protein OS=Arabidopsis th...	[more]
B5BRD4\|B5BRD4_ARATH	3.855e-8	53.23	Putative disease resistance protein OS=Arabidopsis...	[more]
Q570X7\|Q570X7_ARATH	1.121e-7	51.67	Viral resistance protein OS=Arabidopsis thaliana G...	[more]
Q3ECM3\|Q3ECM3_ARATH	1.121e-7	51.67	Putative uncharacterized protein At1g58807.1 OS=Ar...	[more]
Q0WNF7\|Q0WNF7_ARATH	1.121e-7	51.67	PRM1 homolog OS=Arabidopsis thaliana GN=At1g59124 ...	[more]
Q0WLD0\|Q0WLD0_ARATH	1.121e-7	51.67	Putative viral resistance protein (Fragment) OS=Ar...	[more]
B5BRD6\|B5BRD6_ARATH	1.913e-7	51.67	Putative disease resistance gene (Fragment) OS=Ara...	[more]
B5BRD5\|B5BRD5_ARATH	1.913e-7	51.67	Utative disease resistance protein OS=Arabidopsis ...	[more]

BLAST of HO054861 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G58602.2	1.315e-14	61.29	\| Symbols: \| LRR and NB-ARC domains-containing di...	[more]
AT1G58602.1	1.315e-14	61.29	\| Symbols: \| LRR and NB-ARC domains-containing di...	[more]
AT1G59218.2	9.749e-10	51.67	\| Symbols: \| Disease resistance protein (CC-NBS-L...	[more]
AT1G58848.2	9.749e-10	51.67	\| Symbols: \| Disease resistance protein (CC-NBS-L...	[more]
AT1G59218.1	9.749e-10	51.67	\| Symbols: \| Disease resistance protein (CC-NBS-L...	[more]
AT1G58807.1	9.749e-10	51.67	\| Symbols: \| Disease resistance protein (CC-NBS-L...	[more]
AT1G58848.1	9.749e-10	51.67	\| Symbols: \| Disease resistance protein (CC-NBS-L...	[more]
AT1G58400.1	2.172e-9	52.46	\| Symbols: \| Disease resistance protein (CC-NBS-L...	[more]
AT1G59780.1	3.468e-7	33.77	\| Symbols: \| NB-ARC domain-containing disease res...	[more]
AT5G43470.2	4.529e-7	46.77	\| Symbols: RPP8, HRT, RCY1 \| Disease resistance pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 22..31 score

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO054861 ID=HO054861; Name=HO054861; organism=Vaccinium corymbosum; type=EST; length=501bp
GTGTCTTGTTTGATTAATTTCCTACCTGCAACTATATTCCGACCCTCCCC
CGCTCTTGTTCGGTGATTCTAAACTTATAGAATAAGATACTCCTAGTCCT
CTTGTTTGATAAGAGCTGTCCGGGAGAATTTTAAAGGCAATGATCATGAG
ATTACCTGATTTTGGGTGGAAACAGAGGCTTATGAGACTCCTGGCTCTGC
TGTTACATATCTCGTCNAGCATAGTCACAGTTAGACAATTCATCGAACAC
CATCAATGCAGCTCTTTATGTGTACATATATATACATACTCTGTGATTGC
TGAGAGCATTCCTAGTCGTCGAATTCCACAGAAGGAATGTGTTGGACTTT
GTAATAATCTTCTTCCTCCCTTCCGACAATCTCTTCTTCCATCTTTTTGA
TACTATCAAATTTTTTAATGAATAGATAAATCGCAGCTCCATCACGAAGC
TCTTTTAAGTTTGGACAAACTCCGATATAGAGAGTATGAAGAAGGGGCAT
G

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
noIPR	unintegrated