DW043361 | GDV

EST Overview


Name	DW043361
Unique Name	DW043361
Type	EST
Organism	Vaccinium corymbosum (northern highbush blueberry)
Sequence length	451

Libraries


Library Name	Type
LIBEST_018810 Subtracted cDNA library of Vaccinium corymbosum	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
	Vaccinium corymbosum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Vaccinium corymbosum unigene v1	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Swissprot	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL	2011-01-18
BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide	2011-01-25
BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide	2011-02-10
InterProScan analysis for Vaccinium corymbosum unigene v1	2011-01-18
KEGG analysis for Vaccinium corymbosum unigene v1	2011-01-18

Homology

[Close]

BLAST of DW043361 vs. DB:swissprot:display
Match: SYEC_SCHPO (Probable glutamyl-tRNA synthetase, cytoplasmic OS=Schizosaccharomyces pombe GN=SPAC17A5.15c PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.912e-26
Identity = 39/70 (55.71%), Postives = 50/70 (71.43%), Query Frame = -2

Query:   25 EEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 234
            EE+TLMDWGNA V+EI +D +GKV  L   LHL G  K T+ K+TWL +T +   + LV+FDYLITK K+
Sbjct:  575 EEVTLMDWGNAYVREINRDASGKVTSLKLELHLDGDFKKTEKKVTWLADTEDKTPVDLVDFDYLITKDKL 644

HSP 2 Score: 54.6842 bits (130), Expect = 4.912e-26
Identity = 27/66 (40.91%), Postives = 36/66 (54.55%), Query Frame = -1

Query:  248 DKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAES 445
            +KK IDP  PRHTAV     V  T+ +GP   +    PRHKK    G K + +   I I++ DA+S
Sbjct:  505 NKKIIDPVAPRHTAVESGDVVKATIVNGPAAPYAEDRPRHKKNPELGNKKSIFANEILIEQADAQS 570

[Close]

BLAST of DW043361 vs. DB:swissprot:display
Match: SYEC_YEAST (Glutamyl-tRNA synthetase, cytoplasmic OS=Saccharomyces cerevisiae GN=GUS1 PE=1 SV=3)

HSP 1 Score: 68.9366 bits (167), Expect = 4.623e-23
Identity = 34/72 (47.22%), Postives = 52/72 (72.22%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            + EE+TLMDWGN I+ +  K+++G +V     L+L+G  K TK KLTWL +T ++V + LV+FD+LITK ++
Sbjct:  569 VDEEVTLMDWGNVIITK--KNDDGSMV---AKLNLEGDFKKTKHKLTWLADTKDVVPVDLVDFDHLITKDRL 635

HSP 2 Score: 58.5362 bits (140), Expect = 4.623e-23
Identity = 26/70 (37.14%), Postives = 44/70 (62.86%), Query Frame = -1

Query:  236 DKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISVE 445
            +KK IDP  PRHTA+V   ++ L  ++ P++  + + P+HKK    G+K   Y + I +D+ DA+ I+V+
Sbjct:  501 NKKVIDPIAPRHTAIVNPVKIHLEGSEAPQEPKIEMKPKHKKNPAVGEKKVIYYKDIVVDKDDADVINVD 570

[Close]

BLAST of DW043361 vs. DB:swissprot:display
Match: SYEC_DICDI (Probable glutamyl-tRNA synthetase, cytoplasmic OS=Dictyostelium discoideum GN=gluS PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 6.412e-22
Identity = 38/77 (49.35%), Postives = 56/77 (72.73%), Query Frame = -2

Query:   25 NPSQLSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWL-PETSELVNLSLVEFDYLITKKKV 252
            N  +  EE+TLM+WGNAIV+ + ++ENG VV +TG LHL+G VK T  KL+WL  + ++ V + L ++DY+ITK K+
Sbjct:  588 NQIKEGEEVTLMNWGNAIVETLQRNENGDVVSMTGKLHLEGDVKKTDKKLSWLSSDCADTVTVVLQDYDYIITKPKL 664

HSP 2 Score: 43.8986 bits (102), Expect = 6.412e-22
Identity = 20/67 (29.85%), Postives = 37/67 (55.22%), Query Frame = -1

Query:  245 DKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESI 445
            +K  ++PTCPR+TA+ +   V  TL++GP    V+   ++ K    G K  T++ ++ ++  D   I
Sbjct:  524 NKAVLEPTCPRYTAIAKATAVKFTLSNGPTLPEVKDCLKYAKDPSMGTKKVTFSNNLLLEGDDCNQI 590

[Close]

BLAST of DW043361 vs. DB:swissprot:display
Match: SYEP_MOUSE (Bifunctional aminoacyl-tRNA synthetase OS=Mus musculus GN=Eprs PE=2 SV=3)

HSP 1 Score: 46.2098 bits (108), Expect = 1.430e-11
Identity = 22/69 (31.88%), Postives = 41/69 (59.42%), Query Frame = -2

Query:   34 EEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLS--LVEFDYLITK 234
            E +T ++WGN  + +I K+ +GK+  L   L+L+        K+TWL E++  +++    V +++LITK
Sbjct:  564 EMVTFINWGNINITKIHKNADGKITSLDAKLNLENKDYKKTTKITWLAESTHALSIPAVCVTYEHLITK 632

HSP 2 Score: 42.3578 bits (98), Expect = 1.430e-11
Identity = 22/68 (32.35%), Postives = 39/68 (57.35%), Query Frame = -1

Query:  242 DKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESIS 445
            +KK IDP  PR+ A++++  V + + D  E+  ++ + RH K    G K   Y+  ++I+  DAE+ S
Sbjct:  496 NKKVIDPVAPRYVALLKKEVVPVNVLDAQEE--MKEVARHPKNPDVGLKPVWYSPKVFIEGADAETFS 561

[Close]

BLAST of DW043361 vs. DB:swissprot:display
Match: SYEP_HUMAN (Bifunctional aminoacyl-tRNA synthetase OS=Homo sapiens GN=EPRS PE=1 SV=5)

HSP 1 Score: 48.1358 bits (113), Expect = 4.053e-11
Identity = 23/69 (33.33%), Postives = 42/69 (60.87%), Query Frame = -2

Query:   34 EEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSL--VEFDYLITK 234
            E +T ++WGN  + +I K+ +GK++ L   L+L+        K+TWL ET+  + + +  V +++LITK
Sbjct:  564 EMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITK 632

HSP 2 Score: 38.891 bits (89), Expect = 4.053e-11
Identity = 19/68 (27.94%), Postives = 39/68 (57.35%), Query Frame = -1

Query:  242 DKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESIS 445
            +KK IDP  PR+ A++++  + + + +  E+  ++ + +H K    G K   Y+  ++I+  DAE+ S
Sbjct:  496 NKKVIDPVAPRYVALLKKEVIPVNVPEAQEE--MKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFS 561

[Close]

BLAST of DW043361 vs. DB:swissprot:display
Match: SYEP_DROME (Bifunctional aminoacyl-tRNA synthetase OS=Drosophila melanogaster GN=Aats-glupro PE=1 SV=2)

HSP 1 Score: 43.1282 bits (100), Expect = 6.801e-11
Identity = 26/68 (38.24%), Postives = 39/68 (57.35%), Query Frame = -1

Query:  245 DKKFIDPTCPRHTAVVEEGRVMLTLTDGP-EKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESI 445
            +KK IDP  PR+TA+ +E RV++ +     E+  V V   H K E  G+K       I+ID +DAE++
Sbjct:  502 NKKVIDPIAPRYTALEKEKRVIVNVAGAKVERIQVSV---HPKDESLGKKTVLLGPRIYIDYVDAEAL 566

HSP 2 Score: 43.1282 bits (100), Expect = 6.801e-11
Identity = 22/70 (31.43%), Postives = 37/70 (52.86%), Query Frame = -2

Query:   34 EEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWL---PETSELVNLSLVEFDYLITK 234
            E  T ++WGN +++++ KD +G +  +   L+L+       LKLTWL    + S       V FD +I+K
Sbjct:  570 ENATFINWGNILIRKVNKDASGNITSVDAALNLENKDFKKTLKLTWLAVEDDPSAYPPTFCVYFDNIISK 639

[Close]

BLAST of DW043361 vs. DB:swissprot:display
Match: SYEC_ENCCU (Probable glutamyl-tRNA synthetase, cytoplasmic OS=Encephalitozoon cuniculi GN=ECU02_1210 PE=3 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 7.545e-9
Identity = 29/68 (42.65%), Postives = 40/68 (58.82%), Query Frame = -2

Query:   40 QLSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLI 243
            Q +EE TLM+WGNAIVK     ENG V K+   L+  G  K TK K++W+ +    V + L E+  L+
Sbjct:  511 QDNEEFTLMNWGNAIVKSKTV-ENGTVTKMEVSLNPDGDFKLTKNKMSWVSKRGS-VTVELAEYGNLM 576

HSP 2 Score: 30.4166 bits (67), Expect = 7.545e-9
Identity = 17/64 (26.56%), Postives = 32/64 (50.00%), Query Frame = -1

Query:  257 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRID 448
            +++K IDP   R+  V +   V +++ +  E  +   +P+HKK    G K   Y+  I + + D
Sbjct:  445 INRKKIDPVSARYFCVQQRDAVEVSIDNTSE--YTMDVPKHKKNGDLGTKEVFYSSQILLSQED 506

[Close]

BLAST of DW043361 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0005s00300.1 ()

HSP 1 Score: 125.561 bits (314), Expect = 5.189e-57
Identity = 59/72 (81.94%), Postives = 69/72 (95.83%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            ++EEITLMDWGNAIVKEI KD+NG V +L+GVLHL+GSVKTTKLKLTWLP+TSELVNL+LV+FDYLITKKK+
Sbjct:  586 VNEEITLMDWGNAIVKEIEKDQNGNVTRLSGVLHLEGSVKTTKLKLTWLPDTSELVNLTLVDFDYLITKKKL 657

HSP 2 Score: 112.849 bits (281), Expect = 5.189e-57
Identity = 52/70 (74.29%), Postives = 59/70 (84.29%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++KK IDP CPRHTAV+EE RV LTLTDGP++ FVR+IPRHKK EGAG+KATTYT  IWID  DAE ISV
Sbjct:  517 INKKIIDPVCPRHTAVIEEHRVPLTLTDGPKQPFVRIIPRHKKHEGAGEKATTYTNRIWIDHADAELISV 586

[Close]

BLAST of DW043361 vs. Populus trichocarpa peptide v2.0
Match: POPTR_0013s00230.1 ()

HSP 1 Score: 122.865 bits (307), Expect = 4.706e-55
Identity = 58/72 (80.56%), Postives = 68/72 (94.44%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            ++EEITLMDWGNAIVKEI KD++GKV  L+GVLHL+GSVKTTKLK TWLP+TSELVNL+LV+FDYLITKKK+
Sbjct:  577 VNEEITLMDWGNAIVKEIEKDQDGKVTHLSGVLHLEGSVKTTKLKFTWLPDTSELVNLTLVDFDYLITKKKL 648

HSP 2 Score: 108.997 bits (271), Expect = 4.706e-55
Identity = 51/70 (72.86%), Postives = 58/70 (82.86%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++KK IDP CPRHTAV+EE RV LTLT+GPE+ FVR+I RHKK EGAG+K TTYT  IWID  DAESISV
Sbjct:  508 INKKIIDPMCPRHTAVIEEQRVPLTLTNGPEQPFVRIIQRHKKHEGAGEKDTTYTNRIWIDHADAESISV 577

[Close]

BLAST of DW043361 vs. P. persica 1.0 peptide
Match: ppa001925m ()

HSP 1 Score: 114.775 bits (286), Expect = 1.683e-26
Identity = 51/71 (71.83%), Postives = 62/71 (87.32%), Query Frame = -1

Query:  236 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISVE 448
            ++KK IDP CPRHTA+VEE RV+LTL +GPEK FVR+IPRHKK+EGAG+KATTYTR IW++  DAESI V+
Sbjct:  530 INKKIIDPVCPRHTAIVEERRVLLTLINGPEKPFVRIIPRHKKYEGAGEKATTYTRTIWLESADAESIKVD 600

HSP 2 Score: 112.464 bits (280), Expect = 8.351e-26
Identity = 62/113 (54.87%), Postives = 76/113 (67.26%), Query Frame = -2

Query:   25 GQRKHLSASFPGIRNSKVLGKKQQHTRGTYGLTALMLNPSQLSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 363
            G  K      P  +  +  G+K      T  L +      ++ EE+TLMDWGNAIVK I KD++G + KLTGVL+L+GSVKTTKLKLTWLP+T ELV L L+EFDYLITKKKV
Sbjct:  558 GPEKPFVRIIPRHKKYEGAGEKATTYTRTIWLESADAESIKVDEEVTLMDWGNAIVKGIEKDQDGNL-KLTGVLNLEGSVKTTKLKLTWLPQTDELVKLCLMEFDYLITKKKV 669

[Close]

BLAST of DW043361 vs. P. persica 1.0 peptide
Match: ppa003109m ()

HSP 1 Score: 102.449 bits (254), Expect = 8.642e-23
Identity = 51/73 (69.86%), Postives = 62/73 (84.93%), Query Frame = -2

Query:   25 QLSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 243
            ++ EE+TLMDWGNAIVK I KDE+G + KL GVL+L+GS KTTKLKLTWLP+  EL  LSL+EF+YLITKKK+
Sbjct:  470 KVDEEVTLMDWGNAIVKGIEKDEDGNL-KLIGVLNLEGSFKTTKLKLTWLPQIDELPKLSLMEFNYLITKKKL 541

HSP 2 Score: 90.1225 bits (222), Expect = 4.438e-19
Identity = 43/74 (58.11%), Postives = 56/74 (75.68%), Query Frame = -1

Query:  227 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISVE*GN 448
            ++KK IDP CPRHTAV+EEGRV+LTLT+GPEK+FVR+IPRHKK+E AG KA+         ++D E   ++ GN
Sbjct:  417 INKKIIDPVCPRHTAVIEEGRVLLTLTNGPEKSFVRIIPRHKKYERAGAKASI--------KVDEEVTLMDWGN 482

[Close]

BLAST of DW043361 vs. TrEMBL
Match: B9N401|B9N401_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1115489 PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 6.445e-55
Identity = 59/72 (81.94%), Postives = 69/72 (95.83%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            ++EEITLMDWGNAIVKEI KD+NG V +L+GVLHL+GSVKTTKLKLTWLP+TSELVNL+LV+FDYLITKKK+
Sbjct:  586 VNEEITLMDWGNAIVKEIEKDQNGNVTRLSGVLHLEGSVKTTKLKLTWLPDTSELVNLTLVDFDYLITKKKL 657

HSP 2 Score: 112.849 bits (281), Expect = 6.445e-55
Identity = 52/70 (74.29%), Postives = 59/70 (84.29%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++KK IDP CPRHTAV+EE RV LTLTDGP++ FVR+IPRHKK EGAG+KATTYT  IWID  DAE ISV
Sbjct:  517 INKKIIDPVCPRHTAVIEEHRVPLTLTDGPKQPFVRIIPRHKKHEGAGEKATTYTNRIWIDHADAELISV 586

[Close]

BLAST of DW043361 vs. TrEMBL
Match: B9RFS6|B9RFS6_RICCO (Glutamyl-tRNA synthetase, cytoplasmic, putative OS=Ricinus communis GN=RCOM_1437120 PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 4.124e-54
Identity = 60/71 (84.51%), Postives = 68/71 (95.77%), Query Frame = -2

Query:   25 SEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 237
            ++E+TLMDWGNAIVKEIVKDE+GKV +L G LHL+GSVKTTKLKLTWLPETSELVN+SLVEFDYLITKKK+
Sbjct:  587 NKEVTLMDWGNAIVKEIVKDESGKVTELIGALHLEGSVKTTKLKLTWLPETSELVNVSLVEFDYLITKKKL 657

HSP 2 Score: 109.383 bits (272), Expect = 4.124e-54
Identity = 50/71 (70.42%), Postives = 58/71 (81.69%), Query Frame = -1

Query:  236 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISVE 448
            ++KK IDP CPRHTAV+EE RV+LTLTDGPE  FVR+IPRHKK EGAG+KATTYT+ IW+D  DA  I  E
Sbjct:  515 INKKIIDPVCPRHTAVIEERRVLLTLTDGPETPFVRIIPRHKKHEGAGEKATTYTKRIWLDYDDAVCIEKE 585

[Close]

BLAST of DW043361 vs. TrEMBL
Match: A5B9C3|A5B9C3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_020288 PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 1.191e-53
Identity = 57/71 (80.28%), Postives = 67/71 (94.37%), Query Frame = -2

Query:   25 SEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 237
            +EEITLMDWGNAI+KEI KD++G +++L GVLHL+GSVK TKLKLTWLPETSELVNLSL+EFDYLITKKK+
Sbjct:  598 NEEITLMDWGNAIIKEIRKDQDGNIMQLIGVLHLEGSVKATKLKLTWLPETSELVNLSLMEFDYLITKKKL 668

HSP 2 Score: 112.464 bits (280), Expect = 1.191e-53
Identity = 50/69 (72.46%), Postives = 59/69 (85.51%), Query Frame = -1

Query:  242 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESIS 448
            ++KK IDP CPRHTAV++E RV++TL DGPEK FVR+IPRHKKFEGAG+K TTYT+ IWID  DA SIS
Sbjct:  528 INKKIIDPVCPRHTAVIDEKRVLVTLADGPEKPFVRIIPRHKKFEGAGEKCTTYTKRIWIDYADAVSIS 596

[Close]

BLAST of DW043361 vs. TrEMBL
Match: B9I6B1|B9I6B1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_806702 PE=4 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.197e-53
Identity = 58/72 (80.56%), Postives = 68/72 (94.44%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            ++EEITLMDWGNAIVKEI KD++GKV  L+GVLHL+GSVKTTKLK TWLP+TSELVNL+LV+FDYLITKKK+
Sbjct:  279 VNEEITLMDWGNAIVKEIEKDQDGKVTHLSGVLHLEGSVKTTKLKFTWLPDTSELVNLTLVDFDYLITKKKL 350

HSP 2 Score: 111.309 bits (277), Expect = 1.197e-53
Identity = 52/70 (74.29%), Postives = 59/70 (84.29%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++KK IDP CPRHTAV+EE RV LTLT+GPE+ FVR+I RHKK EGAG+KATTYT  IWID  DAESISV
Sbjct:  210 INKKIIDPMCPRHTAVIEEQRVPLTLTNGPEQPFVRIIQRHKKHEGAGEKATTYTNRIWIDHADAESISV 279

[Close]

BLAST of DW043361 vs. TrEMBL
Match: D7UAC3|D7UAC3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_60.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031352001 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 1.567e-53
Identity = 57/71 (80.28%), Postives = 66/71 (92.96%), Query Frame = -2

Query:   25 SEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 237
            +EEITLMDWGNAI+KEI KD++G + +L GVLHL+GSVK TKLKLTWLPETSELVNLSL+EFDYLITKKK+
Sbjct:  138 NEEITLMDWGNAIIKEIRKDQDGNITQLIGVLHLEGSVKATKLKLTWLPETSELVNLSLMEFDYLITKKKL 208

HSP 2 Score: 112.464 bits (280), Expect = 1.567e-53
Identity = 50/69 (72.46%), Postives = 59/69 (85.51%), Query Frame = -1

Query:  242 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESIS 448
            ++KK IDP CPRHTAV++E RV++TL DGPEK FVR+IPRHKKFEGAG+K TTYT+ IWID  DA SIS
Sbjct:   68 INKKIIDPVCPRHTAVIDEKRVLVTLADGPEKPFVRIIPRHKKFEGAGEKCTTYTKRIWIDYADAVSIS 136

[Close]

BLAST of DW043361 vs. TrEMBL
Match: O65253|O65253_ARATH (F21E10.12 protein OS=Arabidopsis thaliana GN=F21E10.12 PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.080e-51
Identity = 57/72 (79.17%), Postives = 66/72 (91.67%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            + EE+TLMDWGNAIVKEI KDE G+V  L+GVL+LQGSVKTTKLKLTWLP+T+ELVNL+L EFDYLITKKK+
Sbjct:  589 VGEEVTLMDWGNAIVKEITKDEEGRVTALSGVLNLQGSVKTTKLKLTWLPDTNELVNLTLTEFDYLITKKKL 660

HSP 2 Score: 106.686 bits (265), Expect = 1.080e-51
Identity = 46/70 (65.71%), Postives = 60/70 (85.71%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++K+ IDP CPRHTAVV E RV+ TLTDGP++ FVR+IP+HKKFEGAG+KATT+T+ IW++  DA +ISV
Sbjct:  520 INKRIIDPVCPRHTAVVAERRVLFTLTDGPDEPFVRMIPKHKKFEGAGEKATTFTKSIWLEEADASAISV 589

[Close]

BLAST of DW043361 vs. TrEMBL
Match: O82462|O82462_ARATH (Glutamyl-tRNA synthetase OS=Arabidopsis thaliana GN=At5g26707 PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.080e-51
Identity = 57/72 (79.17%), Postives = 66/72 (91.67%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            + EE+TLMDWGNAIVKEI KDE G+V  L+GVL+LQGSVKTTKLKLTWLP+T+ELVNL+L EFDYLITKKK+
Sbjct:  580 VGEEVTLMDWGNAIVKEITKDEEGRVTALSGVLNLQGSVKTTKLKLTWLPDTNELVNLTLTEFDYLITKKKL 651

HSP 2 Score: 106.686 bits (265), Expect = 1.080e-51
Identity = 46/70 (65.71%), Postives = 60/70 (85.71%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++K+ IDP CPRHTAVV E RV+ TLTDGP++ FVR+IP+HKKFEGAG+KATT+T+ IW++  DA +ISV
Sbjct:  511 INKRIIDPVCPRHTAVVAERRVLFTLTDGPDEPFVRMIPKHKKFEGAGEKATTFTKSIWLEEADASAISV 580

[Close]

BLAST of DW043361 vs. TrEMBL
Match: D7KBZ2|D7KBZ2_ARALY (Putative uncharacterized protein (Fragment) OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_473931 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 1.082e-51
Identity = 56/72 (77.78%), Postives = 66/72 (91.67%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            + EE+TLMDWGNAIVK+I KDE G+V  L+GVL+LQGSVKTTKLKLTWLP+T+ELVNL+L EFDYLITKKK+
Sbjct:  449 VDEEVTLMDWGNAIVKQITKDEEGRVTALSGVLNLQGSVKTTKLKLTWLPDTNELVNLTLTEFDYLITKKKL 520

HSP 2 Score: 107.842 bits (268), Expect = 1.082e-51
Identity = 46/71 (64.79%), Postives = 61/71 (85.92%), Query Frame = -1

Query:  236 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISVE 448
            ++K+ IDP CPRHTAV+ E RV+ TLTDGP++ FVR+IP+HKKFEGAG+KATT+T+ IWI+  DA +ISV+
Sbjct:  380 INKRIIDPVCPRHTAVIAERRVLFTLTDGPDEPFVRLIPKHKKFEGAGEKATTFTKSIWIEEADASAISVD 450

[Close]

BLAST of DW043361 vs. TrEMBL
Match: Q0WMB4|Q0WMB4_ARATH (Glutamyl-tRNA synthetase (Fragment) OS=Arabidopsis thaliana GN=At5g26710 PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.089e-51
Identity = 57/72 (79.17%), Postives = 66/72 (91.67%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            + EE+TLMDWGNAIVKEI KDE G+V  L+GVL+LQGSVKTTKLKLTWLP+T+ELVNL+L EFDYLITKKK+
Sbjct:  166 VGEEVTLMDWGNAIVKEITKDEEGRVTALSGVLNLQGSVKTTKLKLTWLPDTNELVNLTLTEFDYLITKKKL 237

HSP 2 Score: 106.686 bits (265), Expect = 1.089e-51
Identity = 46/70 (65.71%), Postives = 60/70 (85.71%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++K+ IDP CPRHTAVV E RV+ TLTDGP++ FVR+IP+HKKFEGAG+KATT+T+ IW++  DA +ISV
Sbjct:   97 INKRIIDPVCPRHTAVVAERRVLFTLTDGPDEPFVRMIPKHKKFEGAGEKATTFTKSIWLEEADASAISV 166

[Close]

BLAST of DW043361 vs. TrEMBL
Match: D7M5B1|D7M5B1_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489491 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 2.392e-51
Identity = 56/72 (77.78%), Postives = 66/72 (91.67%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            + EE+TLMDWGNAIVKEI KD+ G+V  L+GVL+LQGSVKTTKLKLTWLP+T+ELVNL+L EFDYLITKKK+
Sbjct:  580 VGEEVTLMDWGNAIVKEITKDKEGRVTALSGVLNLQGSVKTTKLKLTWLPDTNELVNLTLTEFDYLITKKKL 651

HSP 2 Score: 107.071 bits (266), Expect = 2.392e-51
Identity = 46/70 (65.71%), Postives = 60/70 (85.71%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++K+ IDP CPRHTAV+ E RV+ TLTDGP++ FVR+IP+HKKFEGAG+KATT+T+ IWI+  DA +ISV
Sbjct:  511 INKRIIDPVCPRHTAVIAERRVLFTLTDGPDEPFVRLIPKHKKFEGAGEKATTFTKSIWIEEADASAISV 580

[Close]

BLAST of DW043361 vs. Vitis vinifera peptide
Match: GSVIVT01031352001 (assembled CDS)

HSP 1 Score: 121.324 bits (303), Expect = 7.807e-56
Identity = 57/71 (80.28%), Postives = 66/71 (92.96%), Query Frame = -2

Query:   25 SEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 237
            +EEITLMDWGNAI+KEI KD++G + +L GVLHL+GSVK TKLKLTWLPETSELVNLSL+EFDYLITKKK+
Sbjct:  138 NEEITLMDWGNAIIKEIRKDQDGNITQLIGVLHLEGSVKATKLKLTWLPETSELVNLSLMEFDYLITKKKL 208

HSP 2 Score: 112.464 bits (280), Expect = 7.807e-56
Identity = 50/69 (72.46%), Postives = 59/69 (85.51%), Query Frame = -1

Query:  242 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESIS 448
            ++KK IDP CPRHTAV++E RV++TL DGPEK FVR+IPRHKKFEGAG+K TTYT+ IWID  DA SIS
Sbjct:   68 INKKIIDPVCPRHTAVIDEKRVLVTLADGPEKPFVRIIPRHKKFEGAGEKCTTYTKRIWIDYADAVSIS 136

[Close]

BLAST of DW043361 vs. TAIR peptide
Match: AT5G26710.1 (| Symbols: | Glutamyl/glutaminyl-tRNA synthetase, class Ic | chr5:9305673-9308247 FORWARD LENGTH=719)

HSP 1 Score: 120.939 bits (302), Expect = 7.701e-54
Identity = 57/72 (79.17%), Postives = 66/72 (91.67%), Query Frame = -2

Query:   25 LSEEITLMDWGNAIVKEIVKDENGKVVKLTGVLHLQGSVKTTKLKLTWLPETSELVNLSLVEFDYLITKKKV 240
            + EE+TLMDWGNAIVKEI KDE G+V  L+GVL+LQGSVKTTKLKLTWLP+T+ELVNL+L EFDYLITKKK+
Sbjct:  580 VGEEVTLMDWGNAIVKEITKDEEGRVTALSGVLNLQGSVKTTKLKLTWLPDTNELVNLTLTEFDYLITKKKL 651

HSP 2 Score: 106.686 bits (265), Expect = 7.701e-54
Identity = 46/70 (65.71%), Postives = 60/70 (85.71%), Query Frame = -1

Query:  239 MDKKFIDPTCPRHTAVVEEGRVMLTLTDGPEKAFVRVIPRHKKFEGAGQKATTYTRHIWIDRIDAESISV 448
            ++K+ IDP CPRHTAVV E RV+ TLTDGP++ FVR+IP+HKKFEGAG+KATT+T+ IW++  DA +ISV
Sbjct:  511 INKRIIDPVCPRHTAVVAERRVLFTLTDGPDEPFVRMIPKHKKFEGAGEKATTFTKSIWLEEADASAISV 580

The following BLAST results are available for this feature:

BLAST of DW043361 vs. DB:swissprot:display
BLAST of DW043361 vs. Populus trichocarpa peptide v2.0
BLAST of DW043361 vs. P. persica 1.0 peptide
BLAST of DW043361 vs. TrEMBL
BLAST of DW043361 vs. Vitis vinifera peptide
BLAST of DW043361 vs. TAIR peptide

BLAST of DW043361 vs. DB:swissprot:display
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
SYEC_SCHPO	4.912e-26	55.71	Probable glutamyl-tRNA synthetase, cytoplasmic OS=...	[more]
SYEC_YEAST	4.623e-23	47.22	Glutamyl-tRNA synthetase, cytoplasmic OS=Saccharom...	[more]
SYEC_DICDI	6.412e-22	49.35	Probable glutamyl-tRNA synthetase, cytoplasmic OS=...	[more]
SYEP_MOUSE	1.430e-11	31.88	Bifunctional aminoacyl-tRNA synthetase OS=Mus musc...	[more]
SYEP_HUMAN	4.053e-11	33.33	Bifunctional aminoacyl-tRNA synthetase OS=Homo sap...	[more]
SYEP_DROME	6.801e-11	38.24	Bifunctional aminoacyl-tRNA synthetase OS=Drosophi...	[more]
SYEC_ENCCU	7.545e-9	42.65	Probable glutamyl-tRNA synthetase, cytoplasmic OS=...	[more]

BLAST of DW043361 vs. Populus trichocarpa peptide v2.0
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Populus trichocarpa v2.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
POPTR_0005s00300.1	5.189e-57	81.94		[more]
POPTR_0013s00230.1	4.706e-55	80.56		[more]

BLAST of DW043361 vs. P. persica 1.0 peptide
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs Ppersica v1.0 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
ppa001925m	1.683e-26	71.83		[more]
ppa003109m	8.642e-23	69.86		[more]

BLAST of DW043361 vs. TrEMBL
Analysis Date: 2011-01-18 (BLAST: Vaccinium corymbosum unigene v1 vs TrEMBL)
Total hits: 10


Match Name	E-value	Identity	Description
B9N401\|B9N401_POPTR	6.445e-55	81.94	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RFS6\|B9RFS6_RICCO	4.124e-54	84.51	Glutamyl-tRNA synthetase, cytoplasmic, putative OS...	[more]
A5B9C3\|A5B9C3_VITVI	1.191e-53	80.28	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9I6B1\|B9I6B1_POPTR	1.197e-53	80.56	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7UAC3\|D7UAC3_VITVI	1.567e-53	80.28	Whole genome shotgun sequence of line PN40024, sca...	[more]
O65253\|O65253_ARATH	1.080e-51	79.17	F21E10.12 protein OS=Arabidopsis thaliana GN=F21E1...	[more]
O82462\|O82462_ARATH	1.080e-51	79.17	Glutamyl-tRNA synthetase OS=Arabidopsis thaliana G...	[more]
D7KBZ2\|D7KBZ2_ARALY	1.082e-51	77.78	Putative uncharacterized protein (Fragment) OS=Ara...	[more]
Q0WMB4\|Q0WMB4_ARATH	1.089e-51	79.17	Glutamyl-tRNA synthetase (Fragment) OS=Arabidopsis...	[more]
D7M5B1\|D7M5B1_ARALY	2.392e-51	77.78	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of DW043361 vs. Vitis vinifera peptide
Analysis Date: 2011-01-25 (BLAST: Vaccinium corymbosum unigene v1 vs Vitis vinifera peptide)
Total hits: 1


Match Name	E-value	Identity	Description
GSVIVT01031352001	7.807e-56	80.28	assembled CDS	[more]

BLAST of DW043361 vs. TAIR peptide
Analysis Date: 2011-02-10 (BLAST: Vaccinium corymbosum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G26710.1	7.701e-54	79.17	\| Symbols: \| Glutamyl/glutaminyl-tRNA synthetase,...	[more]

InterPro

Analysis Name: InterProScan analysis for Vaccinium corymbosum unigene v1
Date Performed: 2011-01-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000924	Glutamyl/glutaminyl-tRNA synthetase, class Ic	PANTHER	PTHR10119	GLUTAMYL/GLUTAMINYL-TRNA SYNTHETASE	coord: 46..114 score: 1.4
IPR000924	Glutamyl/glutaminyl-tRNA synthetase, class Ic	PANTHER	PTHR10119	GLUTAMYL/GLUTAMINYL-TRNA SYNTHETASE	coord: 2..72 score: 1.2
IPR011035	Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain	SUPERFAMILY	50715	Ribosomal protein L25-like	coord: 46..114 score: 3.5
IPR011035	Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain	SUPERFAMILY	50715	Ribosomal protein L25-like	coord: 11..67 score: 2
IPR020056	Ribosomal protein L25/Gln-tRNA synthetase, beta-barrel domain	GENE3D	2.40.240.10	no description	coord: 45..95 score: 8.9
IPR020059	Glutamyl/glutaminyl-tRNA synthetase, class Ic, anti-codon binding domain	PFAM	PF03950	tRNA-synt_1c_C	coord: 45..113 score: 1.1
IPR020059	Glutamyl/glutaminyl-tRNA synthetase, class Ic, anti-codon binding domain	PFAM	PF03950	tRNA-synt_1c_C	coord: 11..69 score: 1.1
None	No IPR available	PANTHER	PTHR10119:SF6	GLUTAMYL-TRNA SYNTHETASE (GLUTAMATE--TRNA LIGASE)	coord: 46..114 score: 1.4
None	No IPR available	PANTHER	PTHR10119:SF6	GLUTAMYL-TRNA SYNTHETASE (GLUTAMATE--TRNA LIGASE)	coord: 2..72 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Vaccinium corymbosum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DW043361 ID=DW043361; Name=DW043361; organism=Vaccinium corymbosum; type=EST; length=451bp
TCGAGCGGCCGCCCGGGCAGGTCATACCTTCTTTTTGGTAATTAGATAGT
CAAACTCCACCAATGAGAGATTCACCAGCTCGCTTGTTTCAGGTAACCAT
GTAAGCTTCAACTTTGTGGTCTTGACAGATCCTTGAAGATGCAAAACTCC
AGTTAACTTCACAACCTTTCCATTCTCGTCTTTCACAATTTCTTTCACTA
TTGCATTGCCCCAATCCATCAAAGTTATTTCCTCACTCAACTGAGATGGA
TTCAGCATCAATGCGGTCAATCCATATGTGCCTCGTGTATGTTGTTGCTT
TTTGCCCAGCACCTTCGAATTTCTTATGCCTGGGAATGACGCGGACAAAT
GCTTTCTCTGGCCCATCGGTTAGGGTTAACATGACACGTCCTTCTTCAAC
AACAGCAGTATGTCTAGGACATGTAGGATCGATAAACTTTTTATCCATCG
G

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0005524	ATP binding
GO:0004812	aminoacyl-tRNA ligase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation
GO:0043039	tRNA aminoacylation
GO:0006418	tRNA aminoacylation for protein translation

Vocabulary: **KEGG ORTHOLOGY**
Term	Definition
K01885	EARS, gltX; glutamyl-tRNA synthetase [EC:6.1.1.17]

Vocabulary: **KEGG PATHWAY**
Term	Definition
ko00730	Thiamine metabolism
ko03010	Ribosome

Vocabulary: **INTERPRO**
Term	Definition
IPR000924	Glu/Gln-tRNA-synth
IPR011035	Ribosomal_bL25/Gln-tRNA_synth
IPR020056	Rbsml_bL25/Gln-tRNA_synth_N
IPR020059	Glu/Gln-tRNA-synth_Ib_codon-bd
noIPR	unintegrated